YPR006C / ICL2

2-methylisocitrate lyase of the mitochondrial matrix, functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR006C / ICL2

Click on Significant Values for Screen Details ID:SGTC_130|Compound:0136-0219|FD-Score:-3.13|P-value:9.62E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.41|P-value:3.73E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.23|P-value:7.16E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:3.38|P-value:4.34E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.66|P-value:1.53E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:3.28|P-value:6.05E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:3.77|P-value:1.02E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.67|P-value:1.40E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.26|P-value:6.50E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.37|P-value:4.49E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.01|P-value:3.82E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:4.14|P-value:2.25E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:5.62|P-value:1.45E-8 ID:SGTC_1503|Compound:4585-0012|FD-Score:3.98|P-value:4.35E-5 ID:SGTC_1526|Compound:st051518|FD-Score:3.54|P-value:2.44E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.47|P-value:3.09E-4 ID:SGTC_1616|Compound:st002734|FD-Score:3.18|P-value:8.68E-4 ID:SGTC_1653|Compound:st012112|FD-Score:5.91|P-value:2.85E-9 ID:SGTC_1719|Compound:st032227|FD-Score:4.22|P-value:1.57E-5 ID:SGTC_1724|Compound:st037281|FD-Score:4.59|P-value:3.06E-6 ID:SGTC_1759|Compound:st043059|FD-Score:5.55|P-value:2.25E-8 ID:SGTC_1763|Compound:st044482|FD-Score:3.22|P-value:7.47E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.46|P-value:3.29E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.48|P-value:3.03E-4 ID:SGTC_1837|Compound:st055472|FD-Score:-4.07|P-value:2.80E-5 ID:SGTC_1843|Compound:st055809|FD-Score:4.20|P-value:1.70E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.51|P-value:2.71E-4 ID:SGTC_1857|Compound:st056007|FD-Score:3.83|P-value:7.89E-5 ID:SGTC_1916|Compound:st067606|FD-Score:4.29|P-value:1.19E-5 ID:SGTC_1930|Compound:st074634|FD-Score:3.20|P-value:8.06E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.59|P-value:1.98E-4 ID:SGTC_1980|Compound:st071811|FD-Score:3.59|P-value:2.04E-4 ID:SGTC_2038|Compound:4100038|FD-Score:4.65|P-value:2.25E-6 ID:SGTC_2040|Compound:5100729|FD-Score:6.18|P-value:5.49E-10 ID:SGTC_2171|Compound:5739187|FD-Score:4.91|P-value:6.36E-7 ID:SGTC_2191|Compound:5911152|FD-Score:3.95|P-value:4.89E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.68|P-value:1.36E-4 ID:SGTC_2534|Compound:helenine|FD-Score:5.37|P-value:5.82E-8 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:3.59|P-value:2.05E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.51|P-value:2.74E-4 ID:SGTC_3021|Compound:9087658|FD-Score:3.54|P-value:2.44E-4 ID:SGTC_3028|Compound:9087778|FD-Score:4.65|P-value:2.23E-6 ID:SGTC_3056|Compound:9094900|FD-Score:3.75|P-value:1.09E-4 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.14|P-value:9.79E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.67|P-value:1.46E-4 ID:SGTC_3201|Compound:9113999|FD-Score:3.30|P-value:5.79E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-3.23|P-value:7.07E-4 ID:SGTC_3209|Compound:9113616|FD-Score:5.02|P-value:3.66E-7 ID:SGTC_3228|Compound:9131799|FD-Score:-3.95|P-value:4.76E-5 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.13|P-value:9.62E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.41|P-value:3.73E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.23|P-value:7.16E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:3.38|P-value:4.34E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.66|P-value:1.53E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:3.28|P-value:6.05E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:3.77|P-value:1.02E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.67|P-value:1.40E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.26|P-value:6.50E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.37|P-value:4.49E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.01|P-value:3.82E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:4.14|P-value:2.25E-5 ID:SGTC_1264|Compound:0783-0040|FD-Score:5.62|P-value:1.45E-8 ID:SGTC_1503|Compound:4585-0012|FD-Score:3.98|P-value:4.35E-5 ID:SGTC_1526|Compound:st051518|FD-Score:3.54|P-value:2.44E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:3.47|P-value:3.09E-4 ID:SGTC_1616|Compound:st002734|FD-Score:3.18|P-value:8.68E-4 ID:SGTC_1653|Compound:st012112|FD-Score:5.91|P-value:2.85E-9 ID:SGTC_1719|Compound:st032227|FD-Score:4.22|P-value:1.57E-5 ID:SGTC_1724|Compound:st037281|FD-Score:4.59|P-value:3.06E-6 ID:SGTC_1759|Compound:st043059|FD-Score:5.55|P-value:2.25E-8 ID:SGTC_1763|Compound:st044482|FD-Score:3.22|P-value:7.47E-4 ID:SGTC_1786|Compound:berberine|FD-Score:3.46|P-value:3.29E-4 ID:SGTC_1807|Compound:st050092|FD-Score:3.48|P-value:3.03E-4 ID:SGTC_1837|Compound:st055472|FD-Score:-4.07|P-value:2.80E-5 ID:SGTC_1843|Compound:st055809|FD-Score:4.20|P-value:1.70E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.51|P-value:2.71E-4 ID:SGTC_1857|Compound:st056007|FD-Score:3.83|P-value:7.89E-5 ID:SGTC_1916|Compound:st067606|FD-Score:4.29|P-value:1.19E-5 ID:SGTC_1930|Compound:st074634|FD-Score:3.20|P-value:8.06E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.59|P-value:1.98E-4 ID:SGTC_1980|Compound:st071811|FD-Score:3.59|P-value:2.04E-4 ID:SGTC_2038|Compound:4100038|FD-Score:4.65|P-value:2.25E-6 ID:SGTC_2040|Compound:5100729|FD-Score:6.18|P-value:5.49E-10 ID:SGTC_2171|Compound:5739187|FD-Score:4.91|P-value:6.36E-7 ID:SGTC_2191|Compound:5911152|FD-Score:3.95|P-value:4.89E-5 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.68|P-value:1.36E-4 ID:SGTC_2534|Compound:helenine|FD-Score:5.37|P-value:5.82E-8 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:3.59|P-value:2.05E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:3.51|P-value:2.74E-4 ID:SGTC_3021|Compound:9087658|FD-Score:3.54|P-value:2.44E-4 ID:SGTC_3028|Compound:9087778|FD-Score:4.65|P-value:2.23E-6 ID:SGTC_3056|Compound:9094900|FD-Score:3.75|P-value:1.09E-4 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.14|P-value:9.79E-4 ID:SGTC_3080|Compound:9117754|FD-Score:3.67|P-value:1.46E-4 ID:SGTC_3201|Compound:9113999|FD-Score:3.30|P-value:5.79E-4 ID:SGTC_3204|Compound:9114183|FD-Score:-3.23|P-value:7.07E-4 ID:SGTC_3209|Compound:9113616|FD-Score:5.02|P-value:3.66E-7 ID:SGTC_3228|Compound:9131799|FD-Score:-3.95|P-value:4.76E-5

Top fitness defect scores for YPR006C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2040 5100729 6.18 123.0 µM 5.49E-10 11 3.24E-10
2 SGTC_1653 st012112 5.91 65.0 µM 2.85E-9 1 1.75E-9
3 SGTC_1264 0783-0040 5.62 158.0 µM 1.45E-8 5 9.34E-9
4 SGTC_1759 st043059 5.55 70.0 µM 2.25E-8 amide catabolism 8 1.47E-8
5 SGTC_2534 helenine 5.37 20.1 µM 5.82E-8 RSC complex & mRNA processing 6 3.88E-8
6 SGTC_3209 9113616 5.02 49.5 µM 3.66E-7 7 2.56E-7
7 SGTC_2171 5739187 4.91 198.1 µM 6.36E-7 copper-dependent oxidative stress 8 4.51E-7
8 SGTC_3028 9087778 4.65 49.5 µM 2.23E-6 6 1.63E-6
9 SGTC_2038 4100038 4.65 31.0 µM 2.25E-6 plasma membrane duress 31 1.65E-6
10 SGTC_1724 st037281 4.59 8.3 µM 3.06E-6 28 2.26E-6
11 SGTC_1916 st067606 4.29 49.2 µM 1.19E-5 27 9.12E-6
12 SGTC_1719 st032227 4.22 49.2 µM 1.57E-5 17 1.21E-5
13 SGTC_1843 st055809 4.20 45.5 µM 1.70E-5 10 1.31E-5
14 SGTC_1177 0983-0204 4.14 23.6 µM 2.25E-5 13 1.75E-5
15 SGTC_1170 0310-0127 4.01 64.8 µM 3.82E-5 26 3.01E-5
16 SGTC_1503 4585-0012 3.98 61.3 µM 4.35E-5 12 3.44E-5
17 SGTC_2191 5911152 3.95 200.0 µM 4.89E-5 16 3.88E-5
18 SGTC_1857 st056007 3.83 79.3 µM 7.89E-5 9 6.34E-5
19 SGTC_854 0422-0053 3.77 192.0 µM 1.02E-4 PDR1 16 8.27E-5
20 SGTC_3056 9094900 3.75 49.5 µM 1.09E-4 29 8.84E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 1.52E-17 YBR028C YPK3 hom An AGC kinase phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner
0.119 5.50E-12 YPR120C CLB5 hom B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase
0.105 1.08E-9 YBR010W HHT1 hom Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation
0.102 3.02E-9 YGL003C CDH1 hom Cell-cycle regulated activator of the anaphase-promoting complex/cyclosome (APC/C), which directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including Cdc20p, Ase1p, Cin8p and Fin1p
0.102 3.53E-9 YGR021W_p YGR021W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.098 1.43E-8 YDR169C-A_p YDR169C-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.089 2.59E-7 YPL123C RNY1 hom Vacuolar RNase of the T(2) family, relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; promotes apoptosis under stress conditions and this function is independent of its catalytic activity
0.085 7.67E-7 YDL009C_p YDL009C_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene
0.080 3.92E-6 YOR027W STI1 hom Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
0.077 8.14E-6 YGL258W VEL1 hom Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has paralog YOR387C
0.077 9.21E-6 YDR318W MCM21 hom Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
0.076 1.13E-5 YKL015W PUT3 hom Transcriptional activator of proline utilization genes, constitutively binds PUT1 and PUT2 promoter sequences as a dimer and undergoes a conformational change to form the active state; differentially phosphorylated in the presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
0.076 1.13E-5 YGL053W PRM8 hom Pheromone-regulated protein with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family
0.075 1.54E-5 YIL057C RGI2 hom Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose
0.072 2.75E-5 YBR114W RAD16 hom Protein that recognizes and binds damaged DNA in an ATP-dependent manner (with Rad7p) during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex