YPR016C / TIF6

Constituent of 66S pre-ribosomal particles, has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR016C / TIF6

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:4.83|P-value:5.65E-5 ID:SGTC_38|Compound:0929-0051|FD-Score:-4.56|P-value:1.41E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-5.03|P-value:3.04E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:-4.50|P-value:1.66E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:4.60|P-value:1.19E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.96|P-value:7.83E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:4.33|P-value:2.67E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:4.08|P-value:5.61E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.04|P-value:6.48E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:-4.17|P-value:4.49E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:-4.48|P-value:1.79E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-4.61|P-value:1.18E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:6.97|P-value:1.27E-8 ID:SGTC_582|Compound:0987-0078|FD-Score:4.54|P-value:1.42E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:3.90|P-value:9.05E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:-4.09|P-value:5.62E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:6.02|P-value:7.59E-7 ID:SGTC_788|Compound:1182-0090|FD-Score:4.50|P-value:1.63E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:5.37|P-value:8.96E-6 ID:SGTC_1136|Compound:pergolide|FD-Score:6.16|P-value:4.35E-7 ID:SGTC_1331|Compound:1472-0049|FD-Score:-3.97|P-value:7.87E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:-3.92|P-value:8.81E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:4.11|P-value:5.08E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.95|P-value:7.95E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-4.05|P-value:6.28E-4 ID:SGTC_1689|Compound:st020265|FD-Score:13.60|P-value:9.55E-28 ID:SGTC_1767|Compound:st044992|FD-Score:4.46|P-value:1.83E-4 ID:SGTC_1958|Compound:st076513|FD-Score:5.23|P-value:1.48E-5 ID:SGTC_1999|Compound:st074831|FD-Score:4.29|P-value:3.03E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.93|P-value:8.39E-4 ID:SGTC_2105|Compound:5350118|FD-Score:4.39|P-value:2.26E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.09|P-value:5.35E-4 ID:SGTC_2294|Compound:7971431|FD-Score:4.86|P-value:5.23E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.62|P-value:1.16E-4 ID:SGTC_2479|Compound:5617353|FD-Score:5.17|P-value:1.81E-5 ID:SGTC_2645|Compound:abamectin|FD-Score:-4.46|P-value:1.86E-4 ID:SGTC_2649|Compound:anisomycin|FD-Score:4.13|P-value:4.76E-4 ID:SGTC_2694|Compound:st077685|FD-Score:4.39|P-value:2.24E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-5.07|P-value:2.61E-5 ID:SGTC_2812|Compound:7970281|FD-Score:7.35|P-value:2.12E-9 ID:SGTC_2849|Compound:9019321|FD-Score:4.00|P-value:6.96E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.66|P-value:9.83E-5 ID:SGTC_3126|Compound:9127922|FD-Score:8.64|P-value:2.59E-12 ID:SGTC_3145|Compound:9085516|FD-Score:4.40|P-value:2.22E-4 ID:SGTC_3247|Compound:9135149|FD-Score:-4.73|P-value:8.03E-5 ID:SGTC_3285|Compound:9112818|FD-Score:-3.95|P-value:8.33E-4 ID:SGTC_3354|Compound:9151612|FD-Score:-3.97|P-value:7.76E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:4.83|P-value:5.65E-5 ID:SGTC_38|Compound:0929-0051|FD-Score:-4.56|P-value:1.41E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-5.03|P-value:3.04E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:-4.50|P-value:1.66E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:4.60|P-value:1.19E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:3.96|P-value:7.83E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:4.33|P-value:2.67E-4 ID:SGTC_136|Compound:0097-0015|FD-Score:4.08|P-value:5.61E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-4.04|P-value:6.48E-4 ID:SGTC_342|Compound:k048-0088|FD-Score:-4.17|P-value:4.49E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:-4.48|P-value:1.79E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-4.61|P-value:1.18E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:6.97|P-value:1.27E-8 ID:SGTC_582|Compound:0987-0078|FD-Score:4.54|P-value:1.42E-4 ID:SGTC_640|Compound:3381-0960|FD-Score:3.90|P-value:9.05E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:-4.09|P-value:5.62E-4 ID:SGTC_782|Compound:0083-0095|FD-Score:6.02|P-value:7.59E-7 ID:SGTC_788|Compound:1182-0090|FD-Score:4.50|P-value:1.63E-4 ID:SGTC_806|Compound:3966-4455|FD-Score:5.37|P-value:8.96E-6 ID:SGTC_1136|Compound:pergolide|FD-Score:6.16|P-value:4.35E-7 ID:SGTC_1331|Compound:1472-0049|FD-Score:-3.97|P-value:7.87E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:-3.92|P-value:8.81E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:4.11|P-value:5.08E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.95|P-value:7.95E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:-4.05|P-value:6.28E-4 ID:SGTC_1689|Compound:st020265|FD-Score:13.60|P-value:9.55E-28 ID:SGTC_1767|Compound:st044992|FD-Score:4.46|P-value:1.83E-4 ID:SGTC_1958|Compound:st076513|FD-Score:5.23|P-value:1.48E-5 ID:SGTC_1999|Compound:st074831|FD-Score:4.29|P-value:3.03E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.93|P-value:8.39E-4 ID:SGTC_2105|Compound:5350118|FD-Score:4.39|P-value:2.26E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.09|P-value:5.35E-4 ID:SGTC_2294|Compound:7971431|FD-Score:4.86|P-value:5.23E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.62|P-value:1.16E-4 ID:SGTC_2479|Compound:5617353|FD-Score:5.17|P-value:1.81E-5 ID:SGTC_2645|Compound:abamectin|FD-Score:-4.46|P-value:1.86E-4 ID:SGTC_2649|Compound:anisomycin|FD-Score:4.13|P-value:4.76E-4 ID:SGTC_2694|Compound:st077685|FD-Score:4.39|P-value:2.24E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-5.07|P-value:2.61E-5 ID:SGTC_2812|Compound:7970281|FD-Score:7.35|P-value:2.12E-9 ID:SGTC_2849|Compound:9019321|FD-Score:4.00|P-value:6.96E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.66|P-value:9.83E-5 ID:SGTC_3126|Compound:9127922|FD-Score:8.64|P-value:2.59E-12 ID:SGTC_3145|Compound:9085516|FD-Score:4.40|P-value:2.22E-4 ID:SGTC_3247|Compound:9135149|FD-Score:-4.73|P-value:8.03E-5 ID:SGTC_3285|Compound:9112818|FD-Score:-3.95|P-value:8.33E-4 ID:SGTC_3354|Compound:9151612|FD-Score:-3.97|P-value:7.76E-4

Top fitness defect scores for YPR016C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1689 st020265 13.60 53.5 µM 9.55E-28 1 2.18E-42 8.96
2 SGTC_3126 9127922 8.64 49.5 µM 2.59E-12 fatty acid desaturase (OLE1) 6 2.80E-18 0.62
3 SGTC_2812 7970281 7.35 58.4 µM 2.12E-9 1 9.57E-14 0.91
4 SGTC_570 0987-0065 6.97 62.6 µM 1.27E-8 60S ribosome export 3 1.53E-12 2.30
5 SGTC_1136 pergolide 6.16 1.1 µM 4.35E-7 6 3.69E-10 0.70
6 SGTC_782 0083-0095 6.02 30.5 µM 7.59E-7 1 8.74E-10 1.10
7 SGTC_806 3966-4455 5.37 5.9 µM 8.96E-6 1 3.96E-8 0.45
8 SGTC_1958 st076513 5.23 41.9 µM 1.48E-5 60S ribosome export 3 8.56E-8 1.07
9 SGTC_2479 5617353 5.17 43.9 µM 1.81E-5 2 1.18E-7 0.84
10 SGTC_2294 7971431 4.86 200.0 µM 5.23E-5 2 6.01E-7 0.67
11 SGTC_37 0566-0070 4.83 1.6 µM 5.65E-5 1 6.76E-7 0.53
12 SGTC_2993 9054257 4.66 71.4 µM 9.83E-5 fatty acid desaturase (OLE1) 23 1.58E-6 0.07
13 SGTC_87 0100-0052 4.60 35.5 µM 1.19E-4 12 2.13E-6 0.06
14 SGTC_582 0987-0078 4.54 156.0 µM 1.42E-4 calcium & mitochondrial duress 7 2.79E-6 0.31
15 SGTC_788 1182-0090 4.50 188.0 µM 1.63E-4 endomembrane recycling 9 3.45E-6 0.04
16 SGTC_1767 st044992 4.46 70.3 µM 1.83E-4 1 4.09E-6 0.43
17 SGTC_3145 9085516 4.40 49.5 µM 2.22E-4 fatty acid desaturase (OLE1) 9 5.52E-6 0.09
18 SGTC_2694 st077685 4.39 81.9 µM 2.24E-4 endomembrane recycling 5 5.61E-6 0.53
19 SGTC_2105 5350118 4.39 200.0 µM 2.26E-4 4 5.68E-6 0.24
20 SGTC_134 0269-0018 4.33 6.9 µM 2.67E-4 14 7.33E-6 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.180 1.15E-25 YHR170W NMD3 het Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
0.158 3.59E-20 YDR188W CCT6 het Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, essential protein that is required for the assembly of actin and tubulins in vivo; contains an ATP-binding motif
0.153 4.33E-19 YPL131W RPL5 het Ribosomal 60S subunit protein L5; homologous to mammalian ribosomal protein L5 and bacterial L18; binds 5S rRNA and is required for 60S subunit assembly
0.128 1.12E-13 YGL055W OLE1 het Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.125 3.14E-13 YPL093W NOG1 het Putative GTPase that associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
0.118 7.87E-12 YKL180W RPL17A het Ribosomal 60S subunit protein L17A; copurifies with the Dam1 complex (aka DASH complex); homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17A has a paralog, RPL17B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.113 6.00E-11 YDR266C HEL2 hom RING finger ubiquitin ligase (E3); involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; computational analysis suggests a role as a transcription factor
0.109 2.34E-10 YOL120C RPL18A het Ribosomal 60S subunit protein L18A; intron of RPL18A pre-mRNA forms stem-loop structures that are a target for Rnt1p cleavage leading to degradation; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18A has a paralog, RPL18B, that arose from the whole genome duplication
0.108 3.66E-10 YPL238C_d YPL238C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
0.100 6.25E-9 YGL099W LSG1 het Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
0.099 8.52E-9 YBR190W_d YBR190W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
0.096 2.30E-8 YMR132C_p JLP2_p hom Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
0.096 2.72E-8 YLR197W NOP56 het Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
0.094 5.35E-8 YDR187C_d YDR187C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
0.091 1.44E-7 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress