HIPHOP chemogenomics database

YPR051W / MAK3

Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus

Zygosity: Homozygous strain

fixedexpanded
Profile for YPR051W / MAK3

Click on Significant Values for Screen Details

Top fitness defect scores for YPR051W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_124	0136-0217	8.34	7.1 µM	1.82E-13	mitochondrial stress	9	3.58E-17
2	SGTC_3344	9148862	7.19	3.9 µM	1.92E-10		1	3.21E-13
3	SGTC_1124	fisetin	7.19	1.2 µM	1.95E-10		3	3.29E-13
4	SGTC_2845	9003024	6.83	43.0 µM	1.39E-9	amide catabolism	11	4.27E-12
5	SGTC_274	sirtinol	6.58	40.1 µM	5.25E-9		4	2.43E-11
6	SGTC_1687	st020778	6.36	55.4 µM	1.57E-8		102	1.01E-10
7	SGTC_2040	5100729	6.27	123.0 µM	2.49E-8		8	1.85E-10
8	SGTC_146	3448-8650	6.06	53.1 µM	6.66E-8	NEO1-PIK1	54	6.69E-10
9	SGTC_872	0588-0139	5.89	234.0 µM	1.48E-7		8	1.90E-9
10	SGTC_1868	st057644	5.76	42.7 µM	2.76E-7		10	4.27E-9
11	SGTC_1812	st052057	5.62	4.3 µM	5.20E-7	calcium & mitochondrial duress	7	9.77E-9
12	SGTC_967	1306-0166	5.33	352.0 µM	1.81E-6		8	4.99E-8
13	SGTC_425	3825-7017	5.29	13.1 µM	2.15E-6		10	6.22E-8
14	SGTC_269	exalamide	5.24	29.0 µM	2.65E-6	ergosterol biosynthesis	6	8.16E-8
15	SGTC_1803	st050068	5.20	52.4 µM	3.05E-6	NEO1-PIK1	108	9.82E-8
16	SGTC_528	3492-0019	5.07	172.0 µM	5.21E-6	calcium & mitochondrial duress	153	1.97E-7
17	SGTC_2941	9056145	4.98	11.2 µM	7.61E-6		8	3.23E-7
18	SGTC_536	3852-0578	4.95	10.6 µM	8.43E-6		10	3.69E-7
19	SGTC_3338	9144300	4.59	39.6 µM	3.29E-5	Golgi	16	2.17E-6
20	SGTC_2349	9023730	4.52	187.1 µM	4.35E-5		12	3.12E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.182	2.67E-26	YDR093W	DNF2	hom	Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
0.165	5.40E-22	YLL040C	VPS13	hom	Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
0.156	1.22E-19	YOR109W	INP53	hom	Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
0.154	2.56E-19	YAL002W	VPS8	hom	Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.153	3.94E-19	YPR050C_d	YPR050C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
0.143	6.72E-17	YDL041W_d	YDL041W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
0.122	1.56E-12	YDR475C	JIP4	hom	Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.118	7.23E-12	YNL246W	VPS75	hom	NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress
0.116	1.62E-11	YJL004C	SYS1	hom	Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.116	1.73E-11	YDL192W	ARF1	hom	ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.114	3.11E-11	YOR089C	VPS21	hom	Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.113	4.46E-11	YEL053C	MAK10	hom	Non-catalytic subunit of N-terminal acetyltransferase of the NatC type, required for replication of dsRNA virus; expression is glucose-repressible
0.113	5.05E-11	YOR070C	GYP1	hom	Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
0.106	7.35E-10	YML062C	MFT1	hom	Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.106	8.50E-10	YDL077C	VAM6	hom	Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p