YPR055W / SEC8

Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YPR055W / SEC8

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:3.40|P-value:4.55E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:3.23|P-value:8.08E-4 ID:SGTC_57|Compound:miconazole|FD-Score:14.80|P-value:1.44E-47 ID:SGTC_65|Compound:0798-0905|FD-Score:3.28|P-value:6.86E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:3.23|P-value:8.13E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:3.38|P-value:4.84E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-3.58|P-value:2.42E-4 ID:SGTC_252|Compound:miconazole|FD-Score:4.01|P-value:4.61E-5 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.27|P-value:1.58E-5 ID:SGTC_286|Compound:k060-0067|FD-Score:4.38|P-value:9.80E-6 ID:SGTC_287|Compound:k060-0039|FD-Score:3.81|P-value:1.01E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:8.97|P-value:1.13E-18 ID:SGTC_312|Compound:4092-0653|FD-Score:12.40|P-value:1.04E-33 ID:SGTC_320|Compound:4092-0846|FD-Score:10.60|P-value:3.77E-25 ID:SGTC_500|Compound:h-89|FD-Score:3.72|P-value:1.46E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.27|P-value:7.06E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:5.49|P-value:4.42E-8 ID:SGTC_536|Compound:3852-0578|FD-Score:-5.50|P-value:4.13E-8 ID:SGTC_542|Compound:3381-0244|FD-Score:3.67|P-value:1.76E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.19|P-value:9.47E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:3.85|P-value:8.89E-5 ID:SGTC_807|Compound:1315-0151|FD-Score:5.05|P-value:4.21E-7 ID:SGTC_1072|Compound:oxiconazole|FD-Score:5.34|P-value:9.72E-8 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.24|P-value:7.96E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.58|P-value:2.44E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.17|P-value:9.91E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:-3.28|P-value:6.86E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.83|P-value:9.41E-5 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.35|P-value:5.46E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.32|P-value:6.15E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-3.19|P-value:9.35E-4 ID:SGTC_1567|Compound:st069303|FD-Score:6.73|P-value:2.65E-11 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.11|P-value:3.06E-5 ID:SGTC_1613|Compound:st002381|FD-Score:5.98|P-value:2.74E-9 ID:SGTC_1624|Compound:st003710|FD-Score:-3.50|P-value:3.17E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.59|P-value:2.29E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-5.51|P-value:3.92E-8 ID:SGTC_2017|Compound:5132927|FD-Score:3.47|P-value:3.58E-4 ID:SGTC_2026|Compound:5148700|FD-Score:5.48|P-value:4.68E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.81|P-value:7.49E-9 ID:SGTC_2313|Compound:7726885|FD-Score:3.36|P-value:5.34E-4 ID:SGTC_2317|Compound:5986382|FD-Score:-4.63|P-value:3.22E-6 ID:SGTC_2323|Compound:6148992|FD-Score:3.64|P-value:1.95E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-3.70|P-value:1.57E-4 ID:SGTC_2427|Compound:5545055|FD-Score:-3.26|P-value:7.34E-4 ID:SGTC_2432|Compound:5630864|FD-Score:-3.18|P-value:9.58E-4 ID:SGTC_2442|Compound:5158906|FD-Score:-3.31|P-value:6.34E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.29|P-value:6.68E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:11.60|P-value:5.95E-30 ID:SGTC_2916|Compound:7875993|FD-Score:3.39|P-value:4.70E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.26|P-value:7.49E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-3.70|P-value:1.54E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.41|P-value:8.52E-6 ID:SGTC_3165|Compound:9101544|FD-Score:-4.03|P-value:4.26E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:3.40|P-value:4.55E-4 ID:SGTC_56|Compound:0957-0365|FD-Score:3.23|P-value:8.08E-4 ID:SGTC_57|Compound:miconazole|FD-Score:14.80|P-value:1.44E-47 ID:SGTC_65|Compound:0798-0905|FD-Score:3.28|P-value:6.86E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:3.23|P-value:8.13E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:3.38|P-value:4.84E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-3.58|P-value:2.42E-4 ID:SGTC_252|Compound:miconazole|FD-Score:4.01|P-value:4.61E-5 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.27|P-value:1.58E-5 ID:SGTC_286|Compound:k060-0067|FD-Score:4.38|P-value:9.80E-6 ID:SGTC_287|Compound:k060-0039|FD-Score:3.81|P-value:1.01E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:8.97|P-value:1.13E-18 ID:SGTC_312|Compound:4092-0653|FD-Score:12.40|P-value:1.04E-33 ID:SGTC_320|Compound:4092-0846|FD-Score:10.60|P-value:3.77E-25 ID:SGTC_500|Compound:h-89|FD-Score:3.72|P-value:1.46E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.27|P-value:7.06E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:5.49|P-value:4.42E-8 ID:SGTC_536|Compound:3852-0578|FD-Score:-5.50|P-value:4.13E-8 ID:SGTC_542|Compound:3381-0244|FD-Score:3.67|P-value:1.76E-4 ID:SGTC_587|Compound:k089-0003|FD-Score:-3.19|P-value:9.47E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:3.85|P-value:8.89E-5 ID:SGTC_807|Compound:1315-0151|FD-Score:5.05|P-value:4.21E-7 ID:SGTC_1072|Compound:oxiconazole|FD-Score:5.34|P-value:9.72E-8 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.24|P-value:7.96E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.58|P-value:2.44E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.17|P-value:9.91E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:-3.28|P-value:6.86E-4 ID:SGTC_1280|Compound:0880-0253|FD-Score:-3.83|P-value:9.41E-5 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.35|P-value:5.46E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.32|P-value:6.15E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:-3.19|P-value:9.35E-4 ID:SGTC_1567|Compound:st069303|FD-Score:6.73|P-value:2.65E-11 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.11|P-value:3.06E-5 ID:SGTC_1613|Compound:st002381|FD-Score:5.98|P-value:2.74E-9 ID:SGTC_1624|Compound:st003710|FD-Score:-3.50|P-value:3.17E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.59|P-value:2.29E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-5.51|P-value:3.92E-8 ID:SGTC_2017|Compound:5132927|FD-Score:3.47|P-value:3.58E-4 ID:SGTC_2026|Compound:5148700|FD-Score:5.48|P-value:4.68E-8 ID:SGTC_2249|Compound:haloperidol|FD-Score:5.81|P-value:7.49E-9 ID:SGTC_2313|Compound:7726885|FD-Score:3.36|P-value:5.34E-4 ID:SGTC_2317|Compound:5986382|FD-Score:-4.63|P-value:3.22E-6 ID:SGTC_2323|Compound:6148992|FD-Score:3.64|P-value:1.95E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-3.70|P-value:1.57E-4 ID:SGTC_2427|Compound:5545055|FD-Score:-3.26|P-value:7.34E-4 ID:SGTC_2432|Compound:5630864|FD-Score:-3.18|P-value:9.58E-4 ID:SGTC_2442|Compound:5158906|FD-Score:-3.31|P-value:6.34E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:3.29|P-value:6.68E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:11.60|P-value:5.95E-30 ID:SGTC_2916|Compound:7875993|FD-Score:3.39|P-value:4.70E-4 ID:SGTC_3012|Compound:9080433|FD-Score:3.26|P-value:7.49E-4 ID:SGTC_3052|Compound:9092717|FD-Score:-3.70|P-value:1.54E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.41|P-value:8.52E-6 ID:SGTC_3165|Compound:9101544|FD-Score:-4.03|P-value:4.26E-5

Top fitness defect scores for YPR055W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_57 miconazole 14.80 162.5 nM 1.44E-47 ergosterol depletion effects on membrane 2 6.09E-50 3.47
2 SGTC_312 4092-0653 12.40 6.8 µM 1.04E-33 ergosterol depletion effects on membrane 5 2.29E-35 1.98
3 SGTC_2778 tioconazole 11.60 65.1 nM 5.95E-30 ergosterol depletion effects on membrane 2 2.06E-31 1.04
4 SGTC_320 4092-0846 10.60 5.3 µM 3.77E-25 ergosterol depletion effects on membrane 4 2.32E-26 0.66
5 SGTC_291 4130-1278 8.97 35.5 µM 1.13E-18 Golgi 2 1.50E-19 3.81
6 SGTC_1567 st069303 6.73 68.4 µM 2.65E-11 mitochondrial processes 2 8.39E-12 0.19
7 SGTC_1613 st002381 5.98 52.1 µM 2.74E-9 1 1.10E-9 1.36
8 SGTC_2249 haloperidol 5.81 116.4 µM 7.49E-9 ergosterol depletion effects on membrane 16 3.17E-9 0.01
9 SGTC_533 1222-0222 5.49 6.7 µM 4.42E-8 PDR1 2 2.05E-8 0.04
10 SGTC_2026 5148700 5.48 152.0 µM 4.68E-8 1 2.17E-8 0.62
11 SGTC_1072 oxiconazole 5.34 14.9 nM 9.72E-8 ergosterol depletion effects on membrane 13 4.68E-8 0.23
12 SGTC_807 1315-0151 5.05 48.0 µM 4.21E-7 1 2.18E-7 0.59
13 SGTC_3152 9099253 4.41 49.5 µM 8.52E-6 NEO1-PIK1 22 5.13E-6 0.13
14 SGTC_286 k060-0067 4.38 22.9 µM 9.80E-6 tubulin folding & SWR complex 3 5.95E-6 0.22
15 SGTC_256 clotrimazole 4.27 625.0 nM 1.58E-5 ergosterol depletion effects on membrane 16 9.80E-6 0.10
16 SGTC_1597 7-benzyloxygramine 4.11 47.5 µM 3.06E-5 calcium & mitochondrial duress 52 1.97E-5 0.20
17 SGTC_252 miconazole 4.01 1.3 nM 4.61E-5 ergosterol depletion effects on membrane 14 3.02E-5 0.08
18 SGTC_655 k060-0012 3.85 42.0 µM 8.89E-5 7 6.02E-5 0.02
19 SGTC_287 k060-0039 3.81 29.6 µM 1.01E-4 11 6.87E-5 0.22
20 SGTC_500 h-89 3.72 112.0 µM 1.46E-4 cell wall signaling 10 1.01E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.478 7.64E-191 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.437 3.54E-156 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.428 6.86E-150 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.352 2.40E-98 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.311 3.44E-76 YER008C SEC3 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.304 1.29E-72 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.294 6.25E-68 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.277 3.08E-60 YFL005W SEC4 het Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
0.276 1.05E-59 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.271 1.75E-57 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.271 1.73E-57 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.253 6.03E-50 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.248 3.62E-48 YKL136W_d YKL136W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C
0.243 2.42E-46 YGL116W CDC20 het Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
0.234 4.82E-43 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex