HIPHOP chemogenomics database

YPR066W / UBA3

Protein that acts together with Ula1p to activate Rub1p before its conjugation to proteins (neddylation), which may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern

Zygosity: Homozygous strain

fixedexpanded
Profile for YPR066W / UBA3

Click on Significant Values for Screen Details

Top fitness defect scores for YPR066W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1083	didemethylchlorpromazine sulfoxide	9.74	707.4 nM	8.10E-21	redox potentiating	6	9.82E-23
2	SGTC_1082	camptothecin	7.84	424.9 nM	3.84E-14	DNA damage response	12	2.17E-15
3	SGTC_359	0081-0058	6.56	15.9 µM	2.04E-10	60S ribosome export	9	2.71E-11
4	SGTC_268	camptothecin	5.87	39.6 µM	1.11E-8	DNA damage response	32	2.18E-9
5	SGTC_3036	9090181	5.38	49.5 µM	1.48E-7		1	3.76E-8
6	SGTC_1775	st081588	5.36	35.2 µM	1.63E-7	60S ribosome export	8	4.16E-8
7	SGTC_3299	9121982	4.87	43.1 µM	1.70E-6		11	5.48E-7
8	SGTC_816	0325-0417	4.82	81.3 µM	2.20E-6		4	7.26E-7
9	SGTC_2359	9039622	4.75	200.0 µM	2.94E-6	endomembrane recycling	9	9.98E-7
10	SGTC_772	k072-0172	4.49	35.3 µM	9.38E-6		11	3.57E-6
11	SGTC_3023	9089636	4.32	49.5 µM	1.90E-5		3	7.73E-6
12	SGTC_2589	lobaric acid	4.23	100.0 µM	2.81E-5		21	1.19E-5
13	SGTC_3082	9117008	4.21	49.5 µM	3.05E-5		13	1.30E-5
14	SGTC_87	0100-0052	4.13	35.5 µM	4.09E-5		98	1.79E-5
15	SGTC_1707	st032323	4.09	6.3 µM	4.89E-5	DNA damage response	52	2.18E-5
16	SGTC_59	0330-0160	4.05	1.3 µM	5.66E-5		104	2.56E-5
17	SGTC_824	1304-0008	4.01	100.0 µM	6.67E-5	DNA damage response	40	3.07E-5
18	SGTC_667	1498-1044	3.98	59.0 µM	7.34E-5	RPP1 & pyrimidine depletion	17	3.40E-5
19	SGTC_760	0568-0725	3.92	82.9 µM	9.25E-5		10	4.39E-5
20	SGTC_36	0335-0881	3.88	14.2 µM	1.08E-4	ubiquinone biosynthesis & proteasome	123	5.20E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.260	6.84E-53	YPL251W_d	YPL251W_d	het	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.249	1.05E-48	YBL088C	TEL1	hom	Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.225	9.70E-40	YPL252C	YAH1	het	Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.217	5.18E-37	YBR099C_d	YBR099C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.215	1.46E-36	YDR386W	MUS81	hom	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.211	5.79E-35	YDL059C	RAD59	hom	Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.199	2.54E-31	YDR431W_d	YDR431W_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.168	1.27E-22	YBR098W	MMS4	hom	Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.151	1.42E-18	YGL175C	SAE2	hom	Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.149	3.81E-18	YDR076W	RAD55	hom	Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.143	9.50E-17	YGL165C_d	YGL165C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W
0.139	6.22E-16	YDR376W	ARH1	het	Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.137	1.79E-15	YLL012W	YEH1	hom	Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.124	4.83E-13	YDL049C	KNH1	hom	Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.119	4.70E-12	YDR430C	CYM1	hom	Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology