HIPHOP chemogenomics database
YPR074C / TKL1 
Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YPR074C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.459 | 2.33E-174 | YJL120W_d | YJL120W_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO |
| 0.448 | 1.03E-165 | YJL121C | RPE1 | hom | D-ribulose-5-phosphate 3-epimerase, catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress |
| 0.285 | 8.85E-64 | YML007W | YAP1 | hom | Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; YAP1 has a paralog, CAD1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress |
| 0.269 | 8.16E-57 | YHR206W | SKN7 | hom | Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; SKN7 has a paralog, HMS2, that arose from the whole genome duplication |
| 0.255 | 4.82E-51 | YNL241C | ZWF1 | hom | Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adapting to oxidatve stress; homolog of the human G6PD which is deficient in patients with hemolytic anemia; protein abundance increases in response to DNA replication stress |
| 0.246 | 1.57E-47 | YJR049C | UTR1 | hom | ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication |
| 0.234 | 3.74E-43 | YML130C | ERO1 | het | Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds |
| 0.212 | 1.56E-35 | YFL032W_d | YFL032W_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene |
| 0.208 | 4.76E-34 | YML081W | TDA9 | hom | DNA-binding protein, putative transcription factor; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication |
| 0.200 | 1.70E-31 | YMR038C | CCS1 | hom | Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within the N-terminal portion is involved in insertion of copper into Sod1p under conditions of copper deprivation; protein abundance increases in response to DNA replication stress |
| 0.181 | 4.39E-26 | YJR104C | SOD1 | hom | Cytosolic copper-zinc superoxide dismutase; some mutations are analogous to those that cause ALS (amyotrophic lateral sclerosis) in humans; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid |
| 0.161 | 7.95E-21 | YMR161W | HLJ1 | hom | Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ |
| 0.157 | 6.83E-20 | YGL167C | PMR1 | hom | High affinity Ca2+/Mn2+ P-type ATPase required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting and processing; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease |
| 0.151 | 1.29E-18 | YLR292C | SEC72 | hom | Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER |
| 0.150 | 2.78E-18 | YMR021C | MAC1 | hom | Copper-sensing transcription factor involved in regulation of genes required for high affinity copper transport |