YPR091C / NVJ2

Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR091C / NVJ2

Click on Significant Values for Screen Details ID:SGTC_33|Compound:0986-0033|FD-Score:3.18|P-value:1.95E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:2.87|P-value:6.70E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.16|P-value:4.14E-9 ID:SGTC_197|Compound:4544-0043|FD-Score:4.47|P-value:2.99E-7 ID:SGTC_216|Compound:k038-0035|FD-Score:2.98|P-value:4.39E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.16|P-value:4.28E-20 ID:SGTC_291|Compound:4130-1278|FD-Score:3.67|P-value:2.10E-5 ID:SGTC_355|Compound:1311-2252|FD-Score:3.04|P-value:3.47E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:4.54|P-value:2.06E-7 ID:SGTC_528|Compound:3492-0019|FD-Score:4.82|P-value:3.61E-8 ID:SGTC_571|Compound:k831-0106|FD-Score:4.52|P-value:2.31E-7 ID:SGTC_572|Compound:0960-0087|FD-Score:2.95|P-value:5.05E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:3.15|P-value:2.24E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.11|P-value:2.58E-4 ID:SGTC_792|Compound:4534-1957|FD-Score:3.61|P-value:2.81E-5 ID:SGTC_992|Compound:3909-8734|FD-Score:3.09|P-value:2.77E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:-2.98|P-value:4.40E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:-4.28|P-value:8.55E-7 ID:SGTC_1139|Compound:k015-0024|FD-Score:3.96|P-value:5.04E-6 ID:SGTC_1672|Compound:st016598|FD-Score:3.79|P-value:1.20E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:7.45|P-value:4.66E-17 ID:SGTC_1712|Compound:st032288|FD-Score:8.38|P-value:3.98E-21 ID:SGTC_1724|Compound:st037281|FD-Score:-3.16|P-value:2.08E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.90|P-value:6.86E-6 ID:SGTC_1760|Compound:st045453|FD-Score:11.10|P-value:2.77E-35 ID:SGTC_1800|Compound:st050079|FD-Score:3.96|P-value:5.03E-6 ID:SGTC_1801|Compound:st050089|FD-Score:4.20|P-value:1.37E-6 ID:SGTC_1803|Compound:st050068|FD-Score:6.87|P-value:8.59E-15 ID:SGTC_1983|Compound:st071813|FD-Score:2.87|P-value:6.91E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.93|P-value:1.89E-8 ID:SGTC_2036|Compound:5160441|FD-Score:3.20|P-value:1.76E-4 ID:SGTC_2257|Compound:7960413|FD-Score:5.69|P-value:1.08E-10 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.26|P-value:1.37E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.43|P-value:3.86E-7 ID:SGTC_3219|Compound:9131112|FD-Score:4.94|P-value:1.72E-8 ID:SGTC_33|Compound:0986-0033|FD-Score:3.18|P-value:1.95E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:2.87|P-value:6.70E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:5.16|P-value:4.14E-9 ID:SGTC_197|Compound:4544-0043|FD-Score:4.47|P-value:2.99E-7 ID:SGTC_216|Compound:k038-0035|FD-Score:2.98|P-value:4.39E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.16|P-value:4.28E-20 ID:SGTC_291|Compound:4130-1278|FD-Score:3.67|P-value:2.10E-5 ID:SGTC_355|Compound:1311-2252|FD-Score:3.04|P-value:3.47E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:4.54|P-value:2.06E-7 ID:SGTC_528|Compound:3492-0019|FD-Score:4.82|P-value:3.61E-8 ID:SGTC_571|Compound:k831-0106|FD-Score:4.52|P-value:2.31E-7 ID:SGTC_572|Compound:0960-0087|FD-Score:2.95|P-value:5.05E-4 ID:SGTC_746|Compound:1095-0025|FD-Score:3.15|P-value:2.24E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.11|P-value:2.58E-4 ID:SGTC_792|Compound:4534-1957|FD-Score:3.61|P-value:2.81E-5 ID:SGTC_992|Compound:3909-8734|FD-Score:3.09|P-value:2.77E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:-2.98|P-value:4.40E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:-4.28|P-value:8.55E-7 ID:SGTC_1139|Compound:k015-0024|FD-Score:3.96|P-value:5.04E-6 ID:SGTC_1672|Compound:st016598|FD-Score:3.79|P-value:1.20E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:7.45|P-value:4.66E-17 ID:SGTC_1712|Compound:st032288|FD-Score:8.38|P-value:3.98E-21 ID:SGTC_1724|Compound:st037281|FD-Score:-3.16|P-value:2.08E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.90|P-value:6.86E-6 ID:SGTC_1760|Compound:st045453|FD-Score:11.10|P-value:2.77E-35 ID:SGTC_1800|Compound:st050079|FD-Score:3.96|P-value:5.03E-6 ID:SGTC_1801|Compound:st050089|FD-Score:4.20|P-value:1.37E-6 ID:SGTC_1803|Compound:st050068|FD-Score:6.87|P-value:8.59E-15 ID:SGTC_1983|Compound:st071813|FD-Score:2.87|P-value:6.91E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.93|P-value:1.89E-8 ID:SGTC_2036|Compound:5160441|FD-Score:3.20|P-value:1.76E-4 ID:SGTC_2257|Compound:7960413|FD-Score:5.69|P-value:1.08E-10 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.26|P-value:1.37E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.43|P-value:3.86E-7 ID:SGTC_3219|Compound:9131112|FD-Score:4.94|P-value:1.72E-8

Top fitness defect scores for YPR091C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1760 st045453 11.10 10.4 µM 2.77E-35 NEO1-PIK1 25 1.06E-28
2 SGTC_1712 st032288 8.38 53.4 µM 3.98E-21 calcium & mitochondrial duress 38 2.55E-17
3 SGTC_221 trifluoperazine 8.16 10.3 µM 4.28E-20 NEO1-PIK1 83 1.72E-16
4 SGTC_1679 4130-1276 7.45 6.7 µM 4.66E-17 Golgi 16 4.78E-14
5 SGTC_1803 st050068 6.87 52.4 µM 8.59E-15 NEO1-PIK1 56 3.19E-12
6 SGTC_2257 7960413 5.69 200.0 µM 1.08E-10 NEO1-PIK1 17 6.41E-9
7 SGTC_146 3448-8650 5.16 53.1 µM 4.14E-9 NEO1-PIK1 78 1.22E-7
8 SGTC_3219 9131112 4.94 49.5 µM 1.72E-8 Golgi 23 3.85E-7
9 SGTC_2021 4019055 4.93 173.0 µM 1.89E-8 endomembrane recycling 70 4.14E-7
10 SGTC_528 3492-0019 4.82 172.0 µM 3.61E-8 calcium & mitochondrial duress 162 7.00E-7
11 SGTC_515 4092-1064 4.54 102.0 µM 2.06E-7 plasma membrane duress 44 2.86E-6
12 SGTC_571 k831-0106 4.52 44.2 µM 2.31E-7 7 3.14E-6
13 SGTC_197 4544-0043 4.47 41.6 µM 2.99E-7 endomembrane recycling 34 3.86E-6
14 SGTC_3037 9091026 4.43 49.5 µM 3.86E-7 NEO1-PIK1 97 4.75E-6
15 SGTC_1801 st050089 4.20 28.4 µM 1.37E-6 NEO1-PIK1 105 1.33E-5
16 SGTC_1800 st050079 3.96 29.1 µM 5.03E-6 NEO1-PIK1 122 3.80E-5
17 SGTC_1139 k015-0024 3.96 46.9 µM 5.04E-6 PDR1 12 3.80E-5
18 SGTC_1754 st042065 3.90 16.1 µM 6.86E-6 NEO1-PIK1 183 4.89E-5
19 SGTC_1672 st016598 3.79 6.5 µM 1.20E-5 endomembrane recycling 75 7.68E-5
20 SGTC_291 4130-1278 3.67 35.5 µM 2.10E-5 Golgi 20 1.21E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.552 9.33E-267 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.481 4.06E-194 YAR042W SWH1 hom Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction
0.462 2.85E-177 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.461 4.85E-176 YPR092W_d YPR092W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.458 2.41E-173 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.439 1.81E-158 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.438 3.87E-157 YDR491C_d YDR491C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.419 7.89E-143 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.418 7.22E-142 YDR492W IZH1 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.417 3.83E-141 YER120W SCS2 hom Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
0.415 8.44E-140 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.414 2.40E-139 YER119C-A_d YER119C-A_d hom Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
0.409 6.61E-136 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.407 7.71E-134 YIL170W HXT12 hom Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
0.403 3.46E-131 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p