YPR132W / RPS23B

Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR132W / RPS23B

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:3.80|P-value:7.89E-5 ID:SGTC_192|Compound:3455-0609|FD-Score:3.61|P-value:1.62E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:4.02|P-value:3.23E-5 ID:SGTC_223|Compound:busulfan|FD-Score:3.21|P-value:6.77E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.41|P-value:3.30E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.47|P-value:4.82E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.69|P-value:3.59E-6 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.32|P-value:8.16E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:3.42|P-value:3.25E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.11|P-value:9.28E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.21|P-value:6.66E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:3.94|P-value:4.50E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:4.25|P-value:1.25E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.35|P-value:7.89E-6 ID:SGTC_1057|Compound:3448-5381|FD-Score:4.19|P-value:1.57E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:-4.28|P-value:2.14E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.11|P-value:9.46E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:4.23|P-value:1.32E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.41|P-value:3.41E-4 ID:SGTC_1781|Compound:st038725|FD-Score:3.11|P-value:9.54E-4 ID:SGTC_1813|Compound:st051246|FD-Score:-3.45|P-value:5.22E-4 ID:SGTC_1815|Compound:st051655|FD-Score:-3.79|P-value:1.56E-4 ID:SGTC_1847|Compound:st056231|FD-Score:4.71|P-value:1.52E-6 ID:SGTC_2006|Compound:4011487|FD-Score:-3.34|P-value:7.52E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.50|P-value:2.44E-4 ID:SGTC_2194|Compound:5927483|FD-Score:3.30|P-value:5.02E-4 ID:SGTC_2321|Compound:7390090|FD-Score:5.87|P-value:3.34E-9 ID:SGTC_2417|Compound:cerulenin|FD-Score:4.53|P-value:3.50E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.41|P-value:6.04E-4 ID:SGTC_2623|Compound:humulene|FD-Score:3.27|P-value:5.46E-4 ID:SGTC_2876|Compound:9059903|FD-Score:-3.88|P-value:1.08E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.02|P-value:6.34E-5 ID:SGTC_2911|Compound:7942208|FD-Score:-3.96|P-value:7.95E-5 ID:SGTC_2969|Compound:9091408|FD-Score:-3.27|P-value:9.52E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-8.67|P-value:3.12E-17 ID:SGTC_3006|Compound:9075424|FD-Score:3.41|P-value:3.37E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-7.09|P-value:4.41E-12 ID:SGTC_3231|Compound:9132313|FD-Score:3.63|P-value:1.53E-4 ID:SGTC_3238|Compound:9133470|FD-Score:-5.52|P-value:6.07E-8 ID:SGTC_3356|Compound:9151811|FD-Score:3.24|P-value:6.02E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:3.80|P-value:7.89E-5 ID:SGTC_192|Compound:3455-0609|FD-Score:3.61|P-value:1.62E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:4.02|P-value:3.23E-5 ID:SGTC_223|Compound:busulfan|FD-Score:3.21|P-value:6.77E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.41|P-value:3.30E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.47|P-value:4.82E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.69|P-value:3.59E-6 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.32|P-value:8.16E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:3.42|P-value:3.25E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.11|P-value:9.28E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:3.21|P-value:6.66E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:3.94|P-value:4.50E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:4.25|P-value:1.25E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.35|P-value:7.89E-6 ID:SGTC_1057|Compound:3448-5381|FD-Score:4.19|P-value:1.57E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:-4.28|P-value:2.14E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.11|P-value:9.46E-4 ID:SGTC_1402|Compound:3909-7673|FD-Score:4.23|P-value:1.32E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.41|P-value:3.41E-4 ID:SGTC_1781|Compound:st038725|FD-Score:3.11|P-value:9.54E-4 ID:SGTC_1813|Compound:st051246|FD-Score:-3.45|P-value:5.22E-4 ID:SGTC_1815|Compound:st051655|FD-Score:-3.79|P-value:1.56E-4 ID:SGTC_1847|Compound:st056231|FD-Score:4.71|P-value:1.52E-6 ID:SGTC_2006|Compound:4011487|FD-Score:-3.34|P-value:7.52E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.50|P-value:2.44E-4 ID:SGTC_2194|Compound:5927483|FD-Score:3.30|P-value:5.02E-4 ID:SGTC_2321|Compound:7390090|FD-Score:5.87|P-value:3.34E-9 ID:SGTC_2417|Compound:cerulenin|FD-Score:4.53|P-value:3.50E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.41|P-value:6.04E-4 ID:SGTC_2623|Compound:humulene|FD-Score:3.27|P-value:5.46E-4 ID:SGTC_2876|Compound:9059903|FD-Score:-3.88|P-value:1.08E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.02|P-value:6.34E-5 ID:SGTC_2911|Compound:7942208|FD-Score:-3.96|P-value:7.95E-5 ID:SGTC_2969|Compound:9091408|FD-Score:-3.27|P-value:9.52E-4 ID:SGTC_2973|Compound:9094665|FD-Score:-8.67|P-value:3.12E-17 ID:SGTC_3006|Compound:9075424|FD-Score:3.41|P-value:3.37E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-7.09|P-value:4.41E-12 ID:SGTC_3231|Compound:9132313|FD-Score:3.63|P-value:1.53E-4 ID:SGTC_3238|Compound:9133470|FD-Score:-5.52|P-value:6.07E-8 ID:SGTC_3356|Compound:9151811|FD-Score:3.24|P-value:6.02E-4

Top fitness defect scores for YPR132W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2321 7390090 5.87 138.8 µM 3.34E-9 7 2.14E-9
2 SGTC_1847 st056231 4.71 15.0 µM 1.52E-6 19 1.23E-6
3 SGTC_2417 cerulenin 4.53 830.0 nM 3.50E-6 20 2.92E-6
4 SGTC_915 methyl methanesulfonate (MMS) 4.35 110.0 mM 7.89E-6 DNA damage response 32 6.75E-6
5 SGTC_832 4083-0019 4.25 164.0 µM 1.25E-5 translation 74 1.09E-5
6 SGTC_1402 3909-7673 4.23 35.9 µM 1.32E-5 17 1.15E-5
7 SGTC_1057 3448-5381 4.19 134.0 µM 1.57E-5 calcium & mitochondrial duress 22 1.38E-5
8 SGTC_194 1486-1323 4.02 56.9 µM 3.23E-5 19 2.90E-5
9 SGTC_830 4076-0286 3.94 57.8 µM 4.50E-5 translation 58 4.08E-5
10 SGTC_118 0083-0097 3.80 135.3 µM 7.89E-5 translation 151 7.29E-5
11 SGTC_3231 9132313 3.63 49.5 µM 1.53E-4 13 1.44E-4
12 SGTC_192 3455-0609 3.61 60.9 µM 1.62E-4 18 1.53E-4
13 SGTC_2146 5545642 3.50 200.0 µM 2.44E-4 31 2.34E-4
14 SGTC_545 4218-0007 3.42 221.0 µM 3.25E-4 46 3.14E-4
15 SGTC_265 mycophenolic acid 3.41 32.9 µM 3.30E-4 RPP1 & pyrimidine depletion 43 3.19E-4
16 SGTC_3006 9075424 3.41 71.4 µM 3.37E-4 23 3.25E-4
17 SGTC_1479 0549-0158 3.41 134.0 µM 3.41E-4 53 3.30E-4
18 SGTC_2194 5927483 3.30 104.5 µM 5.02E-4 37 4.91E-4
19 SGTC_2623 humulene 3.27 100.0 µM 5.46E-4 48 5.35E-4
20 SGTC_3356 9151811 3.24 4.5 µM 6.02E-4 cell wall 45 5.91E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.202 2.56E-32 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.192 4.14E-29 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.158 2.80E-20 YJL136C RPS21B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
0.146 1.63E-17 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.145 2.83E-17 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.144 4.98E-17 YBR191W RPL21A hom Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.144 6.26E-17 YPL249C-A RPL36B hom Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication
0.140 3.38E-16 YJR063W RPA12 hom RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
0.139 6.55E-16 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.135 4.74E-15 YOR078W BUD21 hom Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
0.131 2.21E-14 YPL239W YAR1 hom Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock
0.130 3.98E-14 YGR162W TIF4631 hom Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
0.130 4.88E-14 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.130 5.77E-14 YGL246C RAI1 hom Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.129 6.94E-14 YGR160W_d YGR160W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data