HIPHOP chemogenomics database

YPR132W / RPS23B

Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit, required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal

Zygosity: Homozygous strain

fixedexpanded
Profile for YPR132W / RPS23B

Click on Significant Values for Screen Details

Top fitness defect scores for YPR132W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2321	7390090	5.87	138.8 µM	3.34E-9		7	2.14E-9
2	SGTC_1847	st056231	4.71	15.0 µM	1.52E-6		19	1.23E-6
3	SGTC_2417	cerulenin	4.53	830.0 nM	3.50E-6		20	2.92E-6
4	SGTC_915	methyl methanesulfonate (MMS)	4.35	110.0 mM	7.89E-6	DNA damage response	32	6.75E-6
5	SGTC_832	4083-0019	4.25	164.0 µM	1.25E-5	translation	74	1.09E-5
6	SGTC_1402	3909-7673	4.23	35.9 µM	1.32E-5		17	1.15E-5
7	SGTC_1057	3448-5381	4.19	134.0 µM	1.57E-5	calcium & mitochondrial duress	22	1.38E-5
8	SGTC_194	1486-1323	4.02	56.9 µM	3.23E-5		19	2.90E-5
9	SGTC_830	4076-0286	3.94	57.8 µM	4.50E-5	translation	58	4.08E-5
10	SGTC_118	0083-0097	3.80	135.3 µM	7.89E-5	translation	151	7.29E-5
11	SGTC_3231	9132313	3.63	49.5 µM	1.53E-4		13	1.44E-4
12	SGTC_192	3455-0609	3.61	60.9 µM	1.62E-4		18	1.53E-4
13	SGTC_2146	5545642	3.50	200.0 µM	2.44E-4		31	2.34E-4
14	SGTC_545	4218-0007	3.42	221.0 µM	3.25E-4		46	3.14E-4
15	SGTC_265	mycophenolic acid	3.41	32.9 µM	3.30E-4	RPP1 & pyrimidine depletion	43	3.19E-4
16	SGTC_3006	9075424	3.41	71.4 µM	3.37E-4		23	3.25E-4
17	SGTC_1479	0549-0158	3.41	134.0 µM	3.41E-4		53	3.30E-4
18	SGTC_2194	5927483	3.30	104.5 µM	5.02E-4		37	4.91E-4
19	SGTC_2623	humulene	3.27	100.0 µM	5.46E-4		48	5.35E-4
20	SGTC_3356	9151811	3.24	4.5 µM	6.02E-4	cell wall	45	5.91E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.202	2.56E-32	YLR048W	RPS0B	hom	Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.192	4.14E-29	YDR140W	MTQ2	hom	S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.158	2.80E-20	YJL136C	RPS21B	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
0.146	1.63E-17	YOR085W	OST3	hom	Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.145	2.83E-17	YGR159C	NSR1	hom	Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.144	4.98E-17	YBR191W	RPL21A	hom	Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.144	6.26E-17	YPL249C-A	RPL36B	hom	Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication
0.140	3.38E-16	YJR063W	RPA12	hom	RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
0.139	6.55E-16	YJL189W	RPL39	hom	Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.135	4.74E-15	YOR078W	BUD21	hom	Component of small ribosomal subunit (SSU) processosome that contains U3 snoRNA; originally isolated as bud-site selection mutant that displays a random budding pattern
0.131	2.21E-14	YPL239W	YAR1	hom	Ankyrin-repeat containing, nucleocytoplasmic shuttling chaperone; prevents aggregation of Rps3p in the cytoplasm, associates with free Rps3p in the cytoplasm and delivers it to the 90S in the nucleus; required for 40S ribosomal subunit export, biogenesis and adaptation to osmotic and oxidative stress; expression repressed by heat shock
0.130	3.98E-14	YGR162W	TIF4631	hom	Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
0.130	4.88E-14	YOR293W	RPS10A	hom	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.130	5.77E-14	YGL246C	RAI1	hom	Nuclear protein with decapping endonuclease activity targeted toward mRNAs with unmethylated 7-methylguanosine cap structures; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; homologous to human DOM3Z
0.129	6.94E-14	YGR160W_d	YGR160W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data