YPR138C / MEP3

Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease

Zygosity: Homozygous strain
fixedexpanded
Profile for YPR138C / MEP3

Click on Significant Values for Screen Details ID:SGTC_129|Compound:0088-0017|FD-Score:-3.66|P-value:5.02E-5 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.04|P-value:6.27E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.40|P-value:1.67E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:4.87|P-value:1.30E-7 ID:SGTC_319|Compound:1348-1596|FD-Score:-3.45|P-value:1.27E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:-3.92|P-value:1.58E-5 ID:SGTC_378|Compound:3970-0959|FD-Score:5.99|P-value:1.20E-10 ID:SGTC_526|Compound:4469-0568|FD-Score:3.09|P-value:5.55E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:-3.46|P-value:1.20E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:4.15|P-value:5.64E-6 ID:SGTC_868|Compound:0527-0199|FD-Score:-4.36|P-value:1.88E-6 ID:SGTC_883|Compound:0850-0245|FD-Score:-3.69|P-value:4.55E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.06|P-value:6.22E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.95|P-value:1.47E-5 ID:SGTC_1273|Compound:0849-0096|FD-Score:4.36|P-value:1.98E-6 ID:SGTC_1322|Compound:1348-1070|FD-Score:3.28|P-value:2.66E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:4.18|P-value:4.99E-6 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.33|P-value:2.18E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.66|P-value:5.60E-5 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.07|P-value:5.98E-4 ID:SGTC_1713|Compound:st033146|FD-Score:5.23|P-value:1.57E-8 ID:SGTC_1937|Compound:st074714|FD-Score:3.02|P-value:7.22E-4 ID:SGTC_1950|Compound:st077106|FD-Score:3.75|P-value:3.67E-5 ID:SGTC_2035|Compound:5154463|FD-Score:2.98|P-value:8.36E-4 ID:SGTC_2051|Compound:5263410|FD-Score:-4.61|P-value:4.92E-7 ID:SGTC_2060|Compound:5238662|FD-Score:4.56|P-value:7.05E-7 ID:SGTC_2289|Compound:7946404|FD-Score:3.46|P-value:1.29E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.15|P-value:4.31E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.27|P-value:2.70E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-4.67|P-value:3.57E-7 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.34|P-value:1.92E-4 ID:SGTC_2425|Compound:5628681|FD-Score:-3.20|P-value:3.36E-4 ID:SGTC_2467|Compound:5474540|FD-Score:3.47|P-value:1.23E-4 ID:SGTC_2475|Compound:5812728|FD-Score:3.45|P-value:1.31E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.94|P-value:1.58E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-5.10|P-value:3.21E-8 ID:SGTC_2760|Compound:etidronate|FD-Score:-4.90|P-value:9.75E-8 ID:SGTC_2826|Compound:7992541|FD-Score:-2.95|P-value:8.61E-4 ID:SGTC_2960|Compound:9079389|FD-Score:3.36|P-value:1.95E-4 ID:SGTC_3062|Compound:9077494|FD-Score:3.09|P-value:5.46E-4 ID:SGTC_3242|Compound:9133940|FD-Score:3.20|P-value:3.57E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.03|P-value:6.37E-4 ID:SGTC_3319|Compound:9138450|FD-Score:3.88|P-value:2.08E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:-3.66|P-value:5.02E-5 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.04|P-value:6.27E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.40|P-value:1.67E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:4.87|P-value:1.30E-7 ID:SGTC_319|Compound:1348-1596|FD-Score:-3.45|P-value:1.27E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:-3.92|P-value:1.58E-5 ID:SGTC_378|Compound:3970-0959|FD-Score:5.99|P-value:1.20E-10 ID:SGTC_526|Compound:4469-0568|FD-Score:3.09|P-value:5.55E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:-3.46|P-value:1.20E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:4.15|P-value:5.64E-6 ID:SGTC_868|Compound:0527-0199|FD-Score:-4.36|P-value:1.88E-6 ID:SGTC_883|Compound:0850-0245|FD-Score:-3.69|P-value:4.55E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.06|P-value:6.22E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:3.95|P-value:1.47E-5 ID:SGTC_1273|Compound:0849-0096|FD-Score:4.36|P-value:1.98E-6 ID:SGTC_1322|Compound:1348-1070|FD-Score:3.28|P-value:2.66E-4 ID:SGTC_1340|Compound:1486-1359|FD-Score:4.18|P-value:4.99E-6 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.33|P-value:2.18E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.66|P-value:5.60E-5 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.07|P-value:5.98E-4 ID:SGTC_1713|Compound:st033146|FD-Score:5.23|P-value:1.57E-8 ID:SGTC_1937|Compound:st074714|FD-Score:3.02|P-value:7.22E-4 ID:SGTC_1950|Compound:st077106|FD-Score:3.75|P-value:3.67E-5 ID:SGTC_2035|Compound:5154463|FD-Score:2.98|P-value:8.36E-4 ID:SGTC_2051|Compound:5263410|FD-Score:-4.61|P-value:4.92E-7 ID:SGTC_2060|Compound:5238662|FD-Score:4.56|P-value:7.05E-7 ID:SGTC_2289|Compound:7946404|FD-Score:3.46|P-value:1.29E-4 ID:SGTC_2330|Compound:7979373|FD-Score:3.15|P-value:4.31E-4 ID:SGTC_2345|Compound:9052315|FD-Score:3.27|P-value:2.70E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-4.67|P-value:3.57E-7 ID:SGTC_2417|Compound:cerulenin|FD-Score:-3.34|P-value:1.92E-4 ID:SGTC_2425|Compound:5628681|FD-Score:-3.20|P-value:3.36E-4 ID:SGTC_2467|Compound:5474540|FD-Score:3.47|P-value:1.23E-4 ID:SGTC_2475|Compound:5812728|FD-Score:3.45|P-value:1.31E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.94|P-value:1.58E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-5.10|P-value:3.21E-8 ID:SGTC_2760|Compound:etidronate|FD-Score:-4.90|P-value:9.75E-8 ID:SGTC_2826|Compound:7992541|FD-Score:-2.95|P-value:8.61E-4 ID:SGTC_2960|Compound:9079389|FD-Score:3.36|P-value:1.95E-4 ID:SGTC_3062|Compound:9077494|FD-Score:3.09|P-value:5.46E-4 ID:SGTC_3242|Compound:9133940|FD-Score:3.20|P-value:3.57E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.03|P-value:6.37E-4 ID:SGTC_3319|Compound:9138450|FD-Score:3.88|P-value:2.08E-5

Top fitness defect scores for YPR138C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_378 3970-0959 5.99 8.3 µM 1.20E-10 1 1.06E-9
2 SGTC_1713 st033146 5.23 12.8 µM 1.57E-8 7 8.37E-8
3 SGTC_247 mechlorethamine 4.87 29.7 µM 1.30E-7 DNA damage response 8 5.54E-7
4 SGTC_2060 5238662 4.56 140.0 µM 7.05E-7 17 2.53E-6
5 SGTC_1273 0849-0096 4.36 14.1 µM 1.98E-6 RPP1 & pyrimidine depletion 8 6.39E-6
6 SGTC_1340 1486-1359 4.18 257.0 µM 4.99E-6 16 1.46E-5
7 SGTC_622 1348-1566 4.15 26.3 µM 5.64E-6 15 1.63E-5
8 SGTC_1272 0839-0021 3.95 27.0 µM 1.47E-5 16 3.86E-5
9 SGTC_2749 bexarotene 3.94 57.4 µM 1.58E-5 24 4.12E-5
10 SGTC_3319 9138450 3.88 16.8 µM 2.08E-5 22 5.27E-5
11 SGTC_1950 st077106 3.75 7.2 µM 3.67E-5 RNA processing & uracil transport 36 8.78E-5
12 SGTC_1483 3453-0561 3.66 55.9 µM 5.60E-5 28 1.28E-4
13 SGTC_2467 5474540 3.47 200.0 µM 1.23E-4 31 2.61E-4
14 SGTC_2289 7946404 3.46 165.1 µM 1.29E-4 113 2.72E-4
15 SGTC_2475 5812728 3.45 15.0 µM 1.31E-4 mitochondrial processes 35 2.76E-4
16 SGTC_238 5hr heat shock (37°C) + nsc-638432 3.40 8.9 µM 1.67E-4 heat shock/prefoldin 64 3.42E-4
17 SGTC_2960 9079389 3.36 6.0 µM 1.95E-4 52 3.94E-4
18 SGTC_1343 1487-0095 3.33 224.0 µM 2.18E-4 20 4.36E-4
19 SGTC_1322 1348-1070 3.28 12.0 µM 2.66E-4 20 5.21E-4
20 SGTC_2345 9052315 3.27 157.4 µM 2.70E-4 47 5.29E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.105 1.01E-9 YEL055C POL5 het DNA Polymerase phi; has sequence similarity to the human MybBP1A and weak sequence similarity to B-type DNA polymerases, not required for chromosomal DNA replication; required for the synthesis of rRNA
0.091 1.42E-7 YJL144W YJL144W hom Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
0.090 1.54E-7 YMR137C PSO2 hom Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress
0.089 2.12E-7 YPL266W DIM1 het Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
0.085 7.08E-7 YNL272C SEC2 het Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
0.085 9.08E-7 YLR059C REX2 hom 3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease
0.084 9.68E-7 YML115C VAN1 hom Component of the mannan polymerase I, which contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
0.083 1.63E-6 YLR434C_d YLR434C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W
0.082 2.03E-6 YDL117W CYK3 hom SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
0.081 2.43E-6 YLR088W GAA1 het Subunit of the GPI (glycosylphosphatidylinositol):protein transamidase complex, removes the GPI-anchoring signal and attaches GPI to proteins in the ER
0.081 2.56E-6 YMR113W FOL3 het Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
0.081 2.81E-6 YHR060W VMA22 hom Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
0.081 2.86E-6 YNL251C NRD1 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
0.078 5.80E-6 YNR062C_p YNR062C_p hom Putative membrane protein of unknown function
0.075 1.58E-5 YGR232W NAS6 hom Proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, which interacts with the Rb tumor suppressor and CDK4/6