YAL056W / GPB2

Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL056W / GPB2

Click on Significant Values for Screen Details ID:SGTC_105|Compound:0111-0055|FD-Score:3.12|P-value:6.19E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:-3.23|P-value:3.97E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:-3.78|P-value:4.25E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.29|P-value:3.11E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.23|P-value:4.19E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.40|P-value:2.20E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:3.40|P-value:2.21E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:3.04|P-value:8.53E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.89|P-value:4.94E-10 ID:SGTC_529|Compound:4076-0302|FD-Score:-2.98|P-value:9.95E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:4.12|P-value:9.99E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:4.00|P-value:1.80E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:5.39|P-value:1.25E-8 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.39|P-value:2.13E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.22|P-value:4.05E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.78|P-value:4.31E-5 ID:SGTC_944|Compound:3370-0224|FD-Score:3.50|P-value:1.51E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.95|P-value:2.25E-5 ID:SGTC_960|Compound:1222-0186|FD-Score:3.05|P-value:8.01E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.57|P-value:1.09E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:5.72|P-value:1.62E-9 ID:SGTC_1078|Compound:idebenone|FD-Score:-4.70|P-value:5.27E-7 ID:SGTC_1082|Compound:camptothecin|FD-Score:5.72|P-value:1.63E-9 ID:SGTC_1097|Compound:itavastatin|FD-Score:3.09|P-value:6.96E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:4.31|P-value:4.15E-6 ID:SGTC_1410|Compound:3909-8106|FD-Score:-3.17|P-value:4.87E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.53|P-value:1.41E-6 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.46|P-value:1.77E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.33|P-value:2.88E-4 ID:SGTC_1715|Compound:st032160|FD-Score:4.34|P-value:3.57E-6 ID:SGTC_1805|Compound:st050013|FD-Score:-3.12|P-value:5.88E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.10|P-value:6.71E-4 ID:SGTC_1957|Compound:st077035|FD-Score:3.55|P-value:1.22E-4 ID:SGTC_1958|Compound:st076513|FD-Score:-3.30|P-value:3.02E-4 ID:SGTC_1973|Compound:st070275|FD-Score:3.17|P-value:5.26E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.25|P-value:3.69E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.29|P-value:4.63E-6 ID:SGTC_2523|Compound:spermine|FD-Score:-3.33|P-value:2.73E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.03|P-value:8.74E-4 ID:SGTC_2624|Compound:nerol|FD-Score:3.43|P-value:1.95E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.49|P-value:1.56E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.01|P-value:8.71E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.00|P-value:9.13E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:3.12|P-value:6.19E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:-3.23|P-value:3.97E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:-3.78|P-value:4.25E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.29|P-value:3.11E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:3.23|P-value:4.19E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.40|P-value:2.20E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:3.40|P-value:2.21E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:3.04|P-value:8.53E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.89|P-value:4.94E-10 ID:SGTC_529|Compound:4076-0302|FD-Score:-2.98|P-value:9.95E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:4.12|P-value:9.99E-6 ID:SGTC_690|Compound:glyoxal|FD-Score:4.00|P-value:1.80E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:5.39|P-value:1.25E-8 ID:SGTC_803|Compound:4488-1585|FD-Score:-3.39|P-value:2.13E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.22|P-value:4.05E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.78|P-value:4.31E-5 ID:SGTC_944|Compound:3370-0224|FD-Score:3.50|P-value:1.51E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.95|P-value:2.25E-5 ID:SGTC_960|Compound:1222-0186|FD-Score:3.05|P-value:8.01E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:3.57|P-value:1.09E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:5.72|P-value:1.62E-9 ID:SGTC_1078|Compound:idebenone|FD-Score:-4.70|P-value:5.27E-7 ID:SGTC_1082|Compound:camptothecin|FD-Score:5.72|P-value:1.63E-9 ID:SGTC_1097|Compound:itavastatin|FD-Score:3.09|P-value:6.96E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:4.31|P-value:4.15E-6 ID:SGTC_1410|Compound:3909-8106|FD-Score:-3.17|P-value:4.87E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:4.53|P-value:1.41E-6 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.46|P-value:1.77E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.33|P-value:2.88E-4 ID:SGTC_1715|Compound:st032160|FD-Score:4.34|P-value:3.57E-6 ID:SGTC_1805|Compound:st050013|FD-Score:-3.12|P-value:5.88E-4 ID:SGTC_1917|Compound:st066507|FD-Score:3.10|P-value:6.71E-4 ID:SGTC_1957|Compound:st077035|FD-Score:3.55|P-value:1.22E-4 ID:SGTC_1958|Compound:st076513|FD-Score:-3.30|P-value:3.02E-4 ID:SGTC_1973|Compound:st070275|FD-Score:3.17|P-value:5.26E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.25|P-value:3.69E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.29|P-value:4.63E-6 ID:SGTC_2523|Compound:spermine|FD-Score:-3.33|P-value:2.73E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.03|P-value:8.74E-4 ID:SGTC_2624|Compound:nerol|FD-Score:3.43|P-value:1.95E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.49|P-value:1.56E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.01|P-value:8.71E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.00|P-value:9.13E-4

Top fitness defect scores for YAL056W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1062 mesoridazine 5.72 14.2 µM 1.62E-9 1 5.32E-9
2 SGTC_1082 camptothecin 5.72 424.9 nM 1.63E-9 DNA damage response 21 5.37E-9
3 SGTC_795 0685-0265 5.39 78.0 µM 1.25E-8 excess fatty acid 8 3.60E-8
4 SGTC_1425 4048-4433 4.53 7.3 µM 1.41E-6 copper-dependent oxidative stress 25 2.99E-6
5 SGTC_1715 st032160 4.34 10.2 µM 3.57E-6 36 7.11E-6
6 SGTC_1266 0828-0227 4.31 32.9 µM 4.15E-6 16 8.20E-6
7 SGTC_2233 sulbentine 4.29 40.4 µM 4.63E-6 45 9.08E-6
8 SGTC_534 k292-0788 4.12 63.4 µM 9.99E-6 mitochondrial processes 25 1.86E-5
9 SGTC_690 glyoxal 4.00 20.3 µM 1.80E-5 80 3.23E-5
10 SGTC_947 3447-0010 3.95 211.0 µM 2.25E-5 13 3.99E-5
11 SGTC_966 1300-0360 3.57 30.2 µM 1.09E-4 heme requiring 37 1.75E-4
12 SGTC_1957 st077035 3.55 62.4 µM 1.22E-4 25 1.94E-4
13 SGTC_944 3370-0224 3.50 231.0 µM 1.51E-4 42 2.37E-4
14 SGTC_2688 kanamycin b 3.49 96.7 µM 1.56E-4 calcium & mitochondrial duress 441 2.44E-4
15 SGTC_1433 4060-0023 3.46 7.6 µM 1.77E-4 tubulin folding & SWR complex 29 2.74E-4
16 SGTC_2624 nerol 3.43 100.0 µM 1.95E-4 21 3.02E-4
17 SGTC_367 0335-0881 3.40 7.1 µM 2.20E-4 ubiquinone biosynthesis & proteasome 108 3.38E-4
18 SGTC_370 0180-0345 3.40 38.7 µM 2.21E-4 DNA intercalators 26 3.39E-4
19 SGTC_1680 diphenhydramine 3.33 100.0 µM 2.88E-4 NEO1 56 4.34E-4
20 SGTC_279 1273-0055 3.23 2.9 µM 4.19E-4 azole & statin 64 6.16E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 5.47E-18 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.129 7.26E-14 YKL176C LST4 hom Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
0.123 1.02E-12 YOR008C-A_p YOR008C-A_p hom Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
0.117 1.14E-11 YLR142W PUT1 hom Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
0.115 2.02E-11 YPR133W-A TOM5 hom Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore
0.110 1.60E-10 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.110 1.65E-10 YCR087C-A_p YCR087C-A_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
0.110 1.78E-10 YBL022C PIM1 hom ATP-dependent Lon protease, involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
0.110 1.87E-10 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.107 5.67E-10 YGR086C PIL1 hom Primary protein component of eisosomes; eisosomes are large immobile cell cortex structures associated with endocytosis; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; detected in phosphorylated state in mitochondria; member of the BAR domain family; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.106 6.37E-10 YIL052C RPL34B hom Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.103 1.99E-9 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.101 4.59E-9 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.097 1.89E-8 YMR004W MVP1 hom Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress
0.096 2.15E-8 YOL081W IRA2 hom GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; similar to human neurofibromin; IRA2 has a paralog, IRA1, that arose from the whole genome duplication