YAL062W / GDH3

NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YAL062W / GDH3

Click on Significant Values for Screen Details ID:SGTC_306|Compound:1672-3436|FD-Score:3.68|P-value:2.64E-5 ID:SGTC_349|Compound:k015-0014|FD-Score:-2.81|P-value:9.78E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.11|P-value:3.11E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-3.25|P-value:1.75E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:4.73|P-value:1.03E-7 ID:SGTC_705|Compound:1124-0212|FD-Score:3.62|P-value:3.62E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.68|P-value:2.51E-5 ID:SGTC_793|Compound:0488-0545|FD-Score:-3.37|P-value:1.04E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:-3.07|P-value:3.57E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.73|P-value:2.14E-5 ID:SGTC_956|Compound:1185-0240|FD-Score:-3.24|P-value:1.83E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.27|P-value:1.69E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:-3.97|P-value:6.28E-6 ID:SGTC_1129|Compound:3453-1384|FD-Score:6.92|P-value:1.44E-14 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.11|P-value:3.21E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.91|P-value:9.01E-6 ID:SGTC_1466|Compound:k213-0079|FD-Score:-3.97|P-value:6.37E-6 ID:SGTC_1469|Compound:k284-2957|FD-Score:-2.89|P-value:7.32E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.02|P-value:4.60E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-2.94|P-value:6.01E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.50|P-value:6.19E-5 ID:SGTC_1729|Compound:st035615|FD-Score:-2.86|P-value:8.33E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-3.74|P-value:1.97E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:2.92|P-value:6.75E-4 ID:SGTC_2160|Compound:5660466|FD-Score:2.89|P-value:7.47E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-3.24|P-value:1.81E-4 ID:SGTC_2440|Compound:5628896|FD-Score:2.94|P-value:6.36E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.78|P-value:1.70E-5 ID:SGTC_2487|Compound:5918373|FD-Score:3.47|P-value:7.05E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.38|P-value:1.04E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.34|P-value:1.25E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:4.67|P-value:1.50E-7 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.77|P-value:1.79E-5 ID:SGTC_2792|Compound:7535235|FD-Score:6.13|P-value:8.25E-12 ID:SGTC_2801|Compound:7907800|FD-Score:-8.61|P-value:1.46E-21 ID:SGTC_2803|Compound:7707263|FD-Score:11.60|P-value:2.95E-37 ID:SGTC_2862|Compound:9027634|FD-Score:-3.92|P-value:7.93E-6 ID:SGTC_2876|Compound:9059903|FD-Score:3.34|P-value:1.24E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.25|P-value:1.77E-4 ID:SGTC_2968|Compound:9090663|FD-Score:-5.40|P-value:1.44E-9 ID:SGTC_3052|Compound:9092717|FD-Score:3.29|P-value:1.54E-4 ID:SGTC_3148|Compound:9097893|FD-Score:-3.21|P-value:2.10E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.06|P-value:3.99E-4 ID:SGTC_3212|Compound:9128307|FD-Score:3.16|P-value:2.65E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-2.93|P-value:6.30E-4 ID:SGTC_3340|Compound:9146634|FD-Score:3.22|P-value:2.07E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:3.68|P-value:2.64E-5 ID:SGTC_349|Compound:k015-0014|FD-Score:-2.81|P-value:9.78E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.11|P-value:3.11E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-3.25|P-value:1.75E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:4.73|P-value:1.03E-7 ID:SGTC_705|Compound:1124-0212|FD-Score:3.62|P-value:3.62E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.68|P-value:2.51E-5 ID:SGTC_793|Compound:0488-0545|FD-Score:-3.37|P-value:1.04E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:-3.07|P-value:3.57E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:3.73|P-value:2.14E-5 ID:SGTC_956|Compound:1185-0240|FD-Score:-3.24|P-value:1.83E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.27|P-value:1.69E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:-3.97|P-value:6.28E-6 ID:SGTC_1129|Compound:3453-1384|FD-Score:6.92|P-value:1.44E-14 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.11|P-value:3.21E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:3.91|P-value:9.01E-6 ID:SGTC_1466|Compound:k213-0079|FD-Score:-3.97|P-value:6.37E-6 ID:SGTC_1469|Compound:k284-2957|FD-Score:-2.89|P-value:7.32E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:3.02|P-value:4.60E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-2.94|P-value:6.01E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.50|P-value:6.19E-5 ID:SGTC_1729|Compound:st035615|FD-Score:-2.86|P-value:8.33E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-3.74|P-value:1.97E-5 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:2.92|P-value:6.75E-4 ID:SGTC_2160|Compound:5660466|FD-Score:2.89|P-value:7.47E-4 ID:SGTC_2161|Compound:5662504|FD-Score:-3.24|P-value:1.81E-4 ID:SGTC_2440|Compound:5628896|FD-Score:2.94|P-value:6.36E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.78|P-value:1.70E-5 ID:SGTC_2487|Compound:5918373|FD-Score:3.47|P-value:7.05E-5 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.38|P-value:1.04E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.34|P-value:1.25E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:4.67|P-value:1.50E-7 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.77|P-value:1.79E-5 ID:SGTC_2792|Compound:7535235|FD-Score:6.13|P-value:8.25E-12 ID:SGTC_2801|Compound:7907800|FD-Score:-8.61|P-value:1.46E-21 ID:SGTC_2803|Compound:7707263|FD-Score:11.60|P-value:2.95E-37 ID:SGTC_2862|Compound:9027634|FD-Score:-3.92|P-value:7.93E-6 ID:SGTC_2876|Compound:9059903|FD-Score:3.34|P-value:1.24E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.25|P-value:1.77E-4 ID:SGTC_2968|Compound:9090663|FD-Score:-5.40|P-value:1.44E-9 ID:SGTC_3052|Compound:9092717|FD-Score:3.29|P-value:1.54E-4 ID:SGTC_3148|Compound:9097893|FD-Score:-3.21|P-value:2.10E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.06|P-value:3.99E-4 ID:SGTC_3212|Compound:9128307|FD-Score:3.16|P-value:2.65E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-2.93|P-value:6.30E-4 ID:SGTC_3340|Compound:9146634|FD-Score:3.22|P-value:2.07E-4

Top fitness defect scores for YAL062W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2803 7707263 11.60 71.4 µM 2.95E-37 1 3.19E-31
2 SGTC_1129 3453-1384 6.92 223.0 µM 1.44E-14 6 2.19E-12
3 SGTC_2792 7535235 6.13 81.8 µM 8.25E-12 fatty acid desaturase (OLE1) 10 4.31E-10
4 SGTC_471 1-acyl-paf 4.73 4.0 µM 1.03E-7 DNA intercalators 18 1.11E-6
5 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 4.67 15.3 µM 1.50E-7 17 1.52E-6
6 SGTC_1380 2291-0730 3.91 164.0 µM 9.01E-6 27 4.67E-5
7 SGTC_2441 5730946 3.78 39.1 µM 1.70E-5 23 7.93E-5
8 SGTC_2639 lactacystin 3.77 53.0 µM 1.79E-5 31 8.30E-5
9 SGTC_811 0416-0114 3.73 265.0 µM 2.14E-5 33 9.61E-5
10 SGTC_306 1672-3436 3.68 11.6 µM 2.64E-5 Golgi 20 1.15E-4
11 SGTC_705 1124-0212 3.62 310.0 µM 3.62E-5 25 1.49E-4
12 SGTC_1728 st035614 3.50 33.8 µM 6.19E-5 76 2.34E-4
13 SGTC_2487 5918373 3.47 44.0 µM 7.05E-5 29 2.61E-4
14 SGTC_2499 avocadenofuran 3.38 51.5 µM 1.04E-4 63 3.61E-4
15 SGTC_2876 9059903 3.34 26.0 µM 1.24E-4 28 4.20E-4
16 SGTC_2568 diosmetin 3.34 100.0 µM 1.25E-4 45 4.21E-4
17 SGTC_3052 9092717 3.29 49.5 µM 1.54E-4 61 5.03E-4
18 SGTC_962 1254-0300 3.27 21.5 µM 1.69E-4 48 5.43E-4
19 SGTC_3340 9146634 3.22 11.1 µM 2.07E-4 38 6.45E-4
20 SGTC_3212 9128307 3.16 49.5 µM 2.65E-4 86 7.94E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.160 1.50E-20 YKL150W MCR1 hom Mitochondrial NADH-cytochrome b5 reductase, involved in ergosterol biosynthesis
0.133 1.15E-14 YLR416C_d YLR416C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.128 9.95E-14 YDR014W RAD61 hom Subunit of a complex (Scc3p, Pds5p, Rad61p) that inhibits sister chromatid cohesion; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; related to the human Wapl protein that controls the association of cohesin with chromatin
0.125 3.62E-13 YDR246W TRS23 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic; human homolog is TRAPPC4
0.120 3.29E-12 YLR018C POM34 hom Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication
0.113 5.55E-11 YLR363W-A YLR363W-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
0.110 1.62E-10 YML070W DAK1 hom Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
0.109 2.44E-10 YOR069W VPS5 hom Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to the late Golgi apparatus; structural component of the retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes
0.108 3.77E-10 YOL075C_p YOL075C_p hom Putative ABC transporter
0.108 3.84E-10 YNL333W SNZ2 hom Member of a stationary phase-induced gene family; transcription of SNZ2 is induced prior to diauxic shift, and also in the absence of thiamin in a Thi2p-dependent manner; forms a coregulated gene pair with SNO2; interacts with Thi11p
0.106 8.10E-10 YIL001W_p YIL001W_p hom Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
0.104 1.32E-9 YDR046C BAP3 hom Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
0.102 2.75E-9 YDR037W KRS1 het Lysyl-tRNA synthetase
0.099 8.17E-9 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.097 1.61E-8 YER168C CCA1 het ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites