YAR008W / SEN34

Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease

Zygosity: Heterozygous strain
fixedexpanded
Profile for YAR008W / SEN34

Click on Significant Values for Screen Details ID:SGTC_141|Compound:0443-0274|FD-Score:-3.20|P-value:9.57E-5 ID:SGTC_269|Compound:exalamide|FD-Score:-3.59|P-value:1.35E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:2.75|P-value:5.38E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:2.72|P-value:6.12E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:2.76|P-value:5.24E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:2.64|P-value:8.38E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:2.89|P-value:3.00E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:2.94|P-value:2.40E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:2.64|P-value:8.60E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.23|P-value:6.49E-5 ID:SGTC_958|Compound:1222-0007|FD-Score:2.82|P-value:4.04E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:2.88|P-value:3.19E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:2.97|P-value:2.08E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:3.13|P-value:1.03E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.23|P-value:2.76E-7 ID:SGTC_1092|Compound:toremifene|FD-Score:5.79|P-value:3.83E-12 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.75|P-value:5.85E-6 ID:SGTC_1108|Compound:betulinic acid|FD-Score:2.67|P-value:7.63E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:-2.66|P-value:9.53E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.06|P-value:1.76E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:5.14|P-value:6.25E-10 ID:SGTC_1259|Compound:0671-0132|FD-Score:-2.77|P-value:6.08E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.15|P-value:9.33E-5 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.02|P-value:2.15E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.37|P-value:4.22E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:3.21|P-value:7.12E-5 ID:SGTC_1649|Compound:st011737|FD-Score:3.32|P-value:4.08E-5 ID:SGTC_1658|Compound:st012944|FD-Score:2.86|P-value:3.39E-4 ID:SGTC_1666|Compound:st014829|FD-Score:-2.67|P-value:9.46E-4 ID:SGTC_1670|Compound:st016595|FD-Score:-2.79|P-value:5.73E-4 ID:SGTC_1733|Compound:st037357|FD-Score:4.05|P-value:8.28E-7 ID:SGTC_1873|Compound:rapamycin|FD-Score:2.70|P-value:6.68E-4 ID:SGTC_1969|Compound:st069479|FD-Score:-3.15|P-value:1.20E-4 ID:SGTC_2117|Compound:5521686|FD-Score:4.90|P-value:3.37E-9 ID:SGTC_2177|Compound:5811945|FD-Score:2.93|P-value:2.53E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.16|P-value:8.76E-5 ID:SGTC_2339|Compound:9000433|FD-Score:3.03|P-value:1.60E-4 ID:SGTC_2447|Compound:5741742|FD-Score:3.13|P-value:1.03E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.62|P-value:8.92E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.37|P-value:3.24E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-2.90|P-value:3.63E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.11|P-value:1.40E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.49|P-value:1.78E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:4.81|P-value:6.13E-9 ID:SGTC_2822|Compound:7994718|FD-Score:2.98|P-value:1.98E-4 ID:SGTC_2856|Compound:9022925|FD-Score:-3.97|P-value:1.75E-6 ID:SGTC_2930|Compound:9013230|FD-Score:-3.95|P-value:1.92E-6 ID:SGTC_2934|Compound:9036370|FD-Score:-3.02|P-value:2.12E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.70|P-value:1.17E-11 ID:SGTC_3025|Compound:9087895|FD-Score:-2.71|P-value:7.83E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-5.68|P-value:1.43E-11 ID:SGTC_3065|Compound:9113888|FD-Score:-3.01|P-value:2.20E-4 ID:SGTC_3067|Compound:9117431|FD-Score:3.28|P-value:4.92E-5 ID:SGTC_3140|Compound:9094243|FD-Score:2.62|P-value:9.36E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.80|P-value:9.80E-9 ID:SGTC_141|Compound:0443-0274|FD-Score:-3.20|P-value:9.57E-5 ID:SGTC_269|Compound:exalamide|FD-Score:-3.59|P-value:1.35E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:2.75|P-value:5.38E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:2.72|P-value:6.12E-4 ID:SGTC_659|Compound:k018-0002|FD-Score:2.76|P-value:5.24E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:2.64|P-value:8.38E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:2.89|P-value:3.00E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:2.94|P-value:2.40E-4 ID:SGTC_861|Compound:0443-0273|FD-Score:2.64|P-value:8.60E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.23|P-value:6.49E-5 ID:SGTC_958|Compound:1222-0007|FD-Score:2.82|P-value:4.04E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:2.88|P-value:3.19E-4 ID:SGTC_990|Compound:3985-0011|FD-Score:2.97|P-value:2.08E-4 ID:SGTC_992|Compound:3909-8734|FD-Score:3.13|P-value:1.03E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:4.23|P-value:2.76E-7 ID:SGTC_1092|Compound:toremifene|FD-Score:5.79|P-value:3.83E-12 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.75|P-value:5.85E-6 ID:SGTC_1108|Compound:betulinic acid|FD-Score:2.67|P-value:7.63E-4 ID:SGTC_1109|Compound:tnp00067|FD-Score:-2.66|P-value:9.53E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.06|P-value:1.76E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:5.14|P-value:6.25E-10 ID:SGTC_1259|Compound:0671-0132|FD-Score:-2.77|P-value:6.08E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:3.15|P-value:9.33E-5 ID:SGTC_1469|Compound:k284-2957|FD-Score:-3.02|P-value:2.15E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-3.37|P-value:4.22E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:3.21|P-value:7.12E-5 ID:SGTC_1649|Compound:st011737|FD-Score:3.32|P-value:4.08E-5 ID:SGTC_1658|Compound:st012944|FD-Score:2.86|P-value:3.39E-4 ID:SGTC_1666|Compound:st014829|FD-Score:-2.67|P-value:9.46E-4 ID:SGTC_1670|Compound:st016595|FD-Score:-2.79|P-value:5.73E-4 ID:SGTC_1733|Compound:st037357|FD-Score:4.05|P-value:8.28E-7 ID:SGTC_1873|Compound:rapamycin|FD-Score:2.70|P-value:6.68E-4 ID:SGTC_1969|Compound:st069479|FD-Score:-3.15|P-value:1.20E-4 ID:SGTC_2117|Compound:5521686|FD-Score:4.90|P-value:3.37E-9 ID:SGTC_2177|Compound:5811945|FD-Score:2.93|P-value:2.53E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.16|P-value:8.76E-5 ID:SGTC_2339|Compound:9000433|FD-Score:3.03|P-value:1.60E-4 ID:SGTC_2447|Compound:5741742|FD-Score:3.13|P-value:1.03E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.62|P-value:8.92E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:3.37|P-value:3.24E-5 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-2.90|P-value:3.63E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-3.11|P-value:1.40E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.49|P-value:1.78E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:4.81|P-value:6.13E-9 ID:SGTC_2822|Compound:7994718|FD-Score:2.98|P-value:1.98E-4 ID:SGTC_2856|Compound:9022925|FD-Score:-3.97|P-value:1.75E-6 ID:SGTC_2930|Compound:9013230|FD-Score:-3.95|P-value:1.92E-6 ID:SGTC_2934|Compound:9036370|FD-Score:-3.02|P-value:2.12E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-5.70|P-value:1.17E-11 ID:SGTC_3025|Compound:9087895|FD-Score:-2.71|P-value:7.83E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-5.68|P-value:1.43E-11 ID:SGTC_3065|Compound:9113888|FD-Score:-3.01|P-value:2.20E-4 ID:SGTC_3067|Compound:9117431|FD-Score:3.28|P-value:4.92E-5 ID:SGTC_3140|Compound:9094243|FD-Score:2.62|P-value:9.36E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.80|P-value:9.80E-9

Top fitness defect scores for YAR008W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1092 toremifene 5.79 6.3 µM 3.83E-12 2 3.50E-9 0.32
2 SGTC_1254 0550-0035 5.14 264.0 µM 6.25E-10 2 1.41E-7 0.37
3 SGTC_2117 5521686 4.90 200.0 µM 3.37E-9 2 4.77E-7 0.66
4 SGTC_2765 myclobutanil 4.81 7.7 µM 6.13E-9 azole & statin 4 7.37E-7 0.40
5 SGTC_996 3970-0799 4.23 1.8 µM 2.76E-7 21 1.17E-5 0.38
6 SGTC_1733 st037357 4.05 54.9 µM 8.28E-7 60S ribosome export 6 2.61E-5 0.07
7 SGTC_2592 piperlongumine 3.62 83.3 µM 8.92E-6 TSC3-RPN4 8 1.47E-4 0.06
8 SGTC_2757 clomiphene 3.49 4.2 µM 1.78E-5 fatty acid desaturase (OLE1) 20 2.43E-4 0.01
9 SGTC_2594 a-mangostin 3.37 100.0 µM 3.24E-5 13 3.78E-4 0.01
10 SGTC_1649 st011737 3.32 51.3 µM 4.08E-5 4 4.47E-4 0.27
11 SGTC_3067 9117431 3.28 49.5 µM 4.92E-5 15 5.12E-4 0.00
12 SGTC_942 3253-1115 3.23 227.0 µM 6.49E-5 19 6.27E-4 0.04
13 SGTC_1578 josamycin 3.21 24.2 µM 7.12E-5 10 6.71E-4 0.05
14 SGTC_2255 7941087 3.16 197.4 µM 8.76E-5 12 7.81E-4 0.08
15 SGTC_1464 k208-0027 3.15 110.0 µM 9.33E-5 10 8.18E-4 0.00
16 SGTC_992 3909-8734 3.13 162.0 µM 1.03E-4 5 8.81E-4 0.16
17 SGTC_2447 5741742 3.13 33.8 µM 1.03E-4 17 8.82E-4 0.00
18 SGTC_2339 9000433 3.03 200.0 µM 1.60E-4 15 0.00121 0.11
19 SGTC_2822 7994718 2.98 71.4 µM 1.98E-4 17 0.00142 0.11
20 SGTC_990 3985-0011 2.97 41.3 µM 2.08E-4 amide catabolism 12 0.00147 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.180 7.19E-26 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.177 4.95E-25 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.173 4.81E-24 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.172 8.05E-24 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.166 5.04E-22 YIL147C SLN1 het Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
0.161 7.28E-21 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.159 1.94E-20 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
0.157 5.08E-20 YIL083C CAB2 het Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.155 1.80E-19 YOR335C ALA1 het Cytoplasmic and mitochondrial alanyl-tRNA synthetase, required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog
0.151 1.70E-18 YKL083W_d YKL083W_d het Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
0.149 3.21E-18 YBL020W RFT1 het Essential integral membrane protein that is required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein
0.148 6.72E-18 YMR270C RRN9 het Protein involved in promoting high level transcription of rDNA, subunit of UAF (upstream activation factor) for RNA polymerase I
0.148 8.22E-18 YER172C BRR2 het RNA-dependent ATPase RNA helicase (DEIH box); required for disruption of U4/U6 base-pairing in native snRNPs to activate the spliceosome for catalysis; homologous to human U5-200kD
0.141 2.43E-16 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.140 4.17E-16 YCR102W-A_d YCR102W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data