YAR019C / CDC15

Protein kinase of the Mitotic Exit Network; localized to the spindle pole bodies at late anaphase; promotes mitotic exit by directly switching on the kinase activity of Dbf2p; required for spindle disassembly after meiosis II; relocalizes to the cytoplasm upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YAR019C / CDC15

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:3.75|P-value:6.55E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.64|P-value:1.02E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.94|P-value:2.48E-5 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.63|P-value:9.16E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.14|P-value:6.00E-4 ID:SGTC_232|Compound:clozapine|FD-Score:3.27|P-value:4.21E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:3.08|P-value:8.57E-4 ID:SGTC_417|Compound:st075190|FD-Score:3.05|P-value:9.52E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:-4.90|P-value:2.33E-7 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.16|P-value:5.52E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.47|P-value:1.71E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.33|P-value:3.44E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.48|P-value:1.68E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:-3.17|P-value:5.42E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:-3.15|P-value:5.78E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:-3.05|P-value:8.12E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.85|P-value:4.32E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.28|P-value:3.54E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-5.53|P-value:6.39E-9 ID:SGTC_1713|Compound:st033146|FD-Score:3.60|P-value:1.22E-4 ID:SGTC_1799|Compound:st045822|FD-Score:3.07|P-value:8.76E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.06|P-value:9.05E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.10|P-value:6.93E-4 ID:SGTC_2158|Compound:5657123|FD-Score:8.90|P-value:4.03E-20 ID:SGTC_2160|Compound:5660466|FD-Score:3.96|P-value:2.61E-5 ID:SGTC_2162|Compound:5663267|FD-Score:-3.13|P-value:6.25E-4 ID:SGTC_2703|Compound:st077852|FD-Score:3.44|P-value:2.30E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.55|P-value:1.26E-4 ID:SGTC_2791|Compound:5663188|FD-Score:8.12|P-value:5.07E-17 ID:SGTC_2814|Compound:7977482|FD-Score:5.11|P-value:8.95E-8 ID:SGTC_2884|Compound:9058502|FD-Score:4.48|P-value:2.41E-6 ID:SGTC_2902|Compound:5787006|FD-Score:3.40|P-value:2.66E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-4.74|P-value:5.32E-7 ID:SGTC_2976|Compound:9009005|FD-Score:-4.38|P-value:3.28E-6 ID:SGTC_2977|Compound:9011197|FD-Score:3.80|P-value:5.27E-5 ID:SGTC_2980|Compound:9014342|FD-Score:8.27|P-value:1.36E-17 ID:SGTC_2983|Compound:9022671|FD-Score:6.88|P-value:9.72E-13 ID:SGTC_2986|Compound:9022445|FD-Score:4.43|P-value:3.07E-6 ID:SGTC_3073|Compound:9114972|FD-Score:3.04|P-value:9.64E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.52|P-value:1.65E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.15|P-value:6.60E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.16|P-value:5.55E-4 ID:SGTC_3207|Compound:9114163|FD-Score:-3.49|P-value:1.62E-4 ID:SGTC_3253|Compound:9135987|FD-Score:-3.26|P-value:3.91E-4 ID:SGTC_3284|Compound:9108171|FD-Score:-3.10|P-value:6.86E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.75|P-value:6.55E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:3.64|P-value:1.02E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-3.94|P-value:2.48E-5 ID:SGTC_170|Compound:4197-0004|FD-Score:-3.63|P-value:9.16E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.14|P-value:6.00E-4 ID:SGTC_232|Compound:clozapine|FD-Score:3.27|P-value:4.21E-4 ID:SGTC_305|Compound:1682-2595|FD-Score:3.08|P-value:8.57E-4 ID:SGTC_417|Compound:st075190|FD-Score:3.05|P-value:9.52E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:-4.90|P-value:2.33E-7 ID:SGTC_541|Compound:4513-1321|FD-Score:-3.16|P-value:5.52E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:-3.47|P-value:1.71E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.33|P-value:3.44E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.48|P-value:1.68E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:-3.17|P-value:5.42E-4 ID:SGTC_1313|Compound:1220-0047|FD-Score:-3.15|P-value:5.78E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:-3.05|P-value:8.12E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:3.85|P-value:4.32E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.28|P-value:3.54E-4 ID:SGTC_1469|Compound:k284-2957|FD-Score:-5.53|P-value:6.39E-9 ID:SGTC_1713|Compound:st033146|FD-Score:3.60|P-value:1.22E-4 ID:SGTC_1799|Compound:st045822|FD-Score:3.07|P-value:8.76E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.06|P-value:9.05E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.10|P-value:6.93E-4 ID:SGTC_2158|Compound:5657123|FD-Score:8.90|P-value:4.03E-20 ID:SGTC_2160|Compound:5660466|FD-Score:3.96|P-value:2.61E-5 ID:SGTC_2162|Compound:5663267|FD-Score:-3.13|P-value:6.25E-4 ID:SGTC_2703|Compound:st077852|FD-Score:3.44|P-value:2.30E-4 ID:SGTC_2783|Compound:7738165|FD-Score:-3.55|P-value:1.26E-4 ID:SGTC_2791|Compound:5663188|FD-Score:8.12|P-value:5.07E-17 ID:SGTC_2814|Compound:7977482|FD-Score:5.11|P-value:8.95E-8 ID:SGTC_2884|Compound:9058502|FD-Score:4.48|P-value:2.41E-6 ID:SGTC_2902|Compound:5787006|FD-Score:3.40|P-value:2.66E-4 ID:SGTC_2905|Compound:7799065|FD-Score:-4.74|P-value:5.32E-7 ID:SGTC_2976|Compound:9009005|FD-Score:-4.38|P-value:3.28E-6 ID:SGTC_2977|Compound:9011197|FD-Score:3.80|P-value:5.27E-5 ID:SGTC_2980|Compound:9014342|FD-Score:8.27|P-value:1.36E-17 ID:SGTC_2983|Compound:9022671|FD-Score:6.88|P-value:9.72E-13 ID:SGTC_2986|Compound:9022445|FD-Score:4.43|P-value:3.07E-6 ID:SGTC_3073|Compound:9114972|FD-Score:3.04|P-value:9.64E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.52|P-value:1.65E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.15|P-value:6.60E-4 ID:SGTC_3145|Compound:9085516|FD-Score:-3.16|P-value:5.55E-4 ID:SGTC_3207|Compound:9114163|FD-Score:-3.49|P-value:1.62E-4 ID:SGTC_3253|Compound:9135987|FD-Score:-3.26|P-value:3.91E-4 ID:SGTC_3284|Compound:9108171|FD-Score:-3.10|P-value:6.86E-4

Top fitness defect scores for YAR019C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2158 5657123 8.90 3.6 µM 4.03E-20 1 2.68E-19 2.41
2 SGTC_2980 9014342 8.27 25.3 µM 1.36E-17 4 6.92E-17 0.68
3 SGTC_2791 5663188 8.12 71.4 µM 5.07E-17 RPP1 & pyrimidine depletion 4 2.42E-16 0.77
4 SGTC_2983 9022671 6.88 13.0 µM 9.72E-13 60S ribosome export 3 2.95E-12 1.79
5 SGTC_2814 7977482 5.11 43.0 µM 8.95E-8 5 1.62E-7 0.34
6 SGTC_2884 9058502 4.48 52.0 µM 2.41E-6 2 3.77E-6 0.95
7 SGTC_2986 9022445 4.43 71.4 µM 3.07E-6 3 4.75E-6 0.07
8 SGTC_2160 5660466 3.96 132.9 µM 2.61E-5 60S ribosome export 15 3.69E-5 0.15
9 SGTC_1368 1852-0141 3.85 284.0 µM 4.32E-5 ERG2 3 5.97E-5 0.03
10 SGTC_2977 9011197 3.80 71.4 µM 5.27E-5 11 7.22E-5 0.13
11 SGTC_57 miconazole 3.75 162.5 nM 6.55E-5 ergosterol depletion effects on membrane 27 8.90E-5 0.01
12 SGTC_58 0957-0393 3.64 9.0 µM 1.02E-4 cell wall 56 1.36E-4 0.08
13 SGTC_1713 st033146 3.60 12.8 µM 1.22E-4 12 1.61E-4 0.07
14 SGTC_3138 9097216 3.52 49.5 µM 1.65E-4 9 2.15E-4 0.10
15 SGTC_2703 st077852 3.44 53.4 µM 2.30E-4 9 2.96E-4 0.01
16 SGTC_2902 5787006 3.40 60.5 µM 2.66E-4 Golgi 9 3.41E-4 0.01
17 SGTC_1133 3473-0247 3.33 50.0 µM 3.44E-4 RSC complex & mRNA processing 62 4.36E-4 0.03
18 SGTC_232 clozapine 3.27 119.0 µM 4.21E-4 NEO1 8 5.29E-4 0.24
19 SGTC_3142 9086498 3.15 49.5 µM 6.60E-4 iron homeostasis 61 8.15E-4 0.02
20 SGTC_305 1682-2595 3.08 20.8 µM 8.57E-4 RNA processing & uracil transport 16 0.00105 0.16

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.152 6.94E-19 YAL003W EFB1 het Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site
0.136 2.23E-15 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.132 1.47E-14 YLR345W_p YLR345W_p hom Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
0.121 1.88E-12 YLR097C HRT3 hom Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
0.115 2.05E-11 YBR052C RFS1 hom Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication
0.113 4.55E-11 YMR164C MSS11 hom Transcription factor involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals
0.113 7.00E-11 YBL081W_p YBL081W_p hom Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport
0.111 1.05E-10 YOR083W WHI5 hom Repressor of G1 transcription that binds to SCB binding factor (SBF) at SCB target promoters in early G1; phosphorylation of Whi5p by the CDK, Cln3p/Cdc28p relieves repression and promoter binding by Whi5; periodically expressed in G1
0.109 2.48E-10 YEL015W EDC3 hom Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress
0.108 4.08E-10 YAL032C PRP45 het Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
0.107 5.18E-10 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.107 5.37E-10 YDR020C_p DAS2_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
0.104 1.94E-9 YHR068W DYS1 het Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric
0.103 2.25E-9 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.098 1.14E-8 YMR095C SNO1 hom Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase