YBR084W / MIS1

Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR084W / MIS1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-2.85|P-value:8.36E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.22|P-value:1.57E-6 ID:SGTC_377|Compound:3970-0964|FD-Score:-3.12|P-value:2.93E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:-3.37|P-value:1.02E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.88|P-value:9.30E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.01|P-value:4.77E-6 ID:SGTC_665|Compound:3914-0008|FD-Score:-2.86|P-value:8.11E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.85|P-value:1.05E-5 ID:SGTC_761|Compound:k216-0224|FD-Score:3.26|P-value:1.58E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:3.65|P-value:2.86E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:4.20|P-value:1.79E-6 ID:SGTC_947|Compound:3447-0010|FD-Score:-2.89|P-value:7.26E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:5.67|P-value:1.91E-10 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.86|P-value:4.19E-8 ID:SGTC_1231|Compound:0242-0792|FD-Score:-2.82|P-value:9.46E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.16|P-value:2.47E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:2.81|P-value:9.52E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.12|P-value:2.88E-4 ID:SGTC_1563|Compound:conessine|FD-Score:2.89|P-value:7.05E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.03|P-value:4.08E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:2.82|P-value:9.31E-4 ID:SGTC_1571|Compound:st070080|FD-Score:-2.88|P-value:7.60E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-2.82|P-value:9.30E-4 ID:SGTC_1770|Compound:st043884|FD-Score:-2.98|P-value:5.16E-4 ID:SGTC_1889|Compound:st059080|FD-Score:2.98|P-value:4.98E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:2.85|P-value:8.13E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.24|P-value:1.80E-4 ID:SGTC_2019|Compound:4016004|FD-Score:5.07|P-value:1.06E-8 ID:SGTC_2021|Compound:4019055|FD-Score:4.16|P-value:2.20E-6 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.88|P-value:9.19E-6 ID:SGTC_2316|Compound:5844804|FD-Score:4.14|P-value:2.46E-6 ID:SGTC_2325|Compound:6158266|FD-Score:-3.54|P-value:4.64E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:2.95|P-value:5.71E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-5.10|P-value:9.41E-9 ID:SGTC_2600|Compound:tangeritin|FD-Score:-4.22|P-value:1.67E-6 ID:SGTC_2693|Compound:st077581|FD-Score:2.97|P-value:5.19E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:2.81|P-value:9.50E-4 ID:SGTC_2803|Compound:7707263|FD-Score:-2.98|P-value:5.12E-4 ID:SGTC_2831|Compound:7999036|FD-Score:2.89|P-value:7.16E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.65|P-value:2.81E-5 ID:SGTC_3224|Compound:9130643|FD-Score:3.02|P-value:4.26E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-2.96|P-value:5.41E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-2.85|P-value:8.36E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:4.22|P-value:1.57E-6 ID:SGTC_377|Compound:3970-0964|FD-Score:-3.12|P-value:2.93E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:-3.37|P-value:1.02E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.88|P-value:9.30E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.01|P-value:4.77E-6 ID:SGTC_665|Compound:3914-0008|FD-Score:-2.86|P-value:8.11E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:3.85|P-value:1.05E-5 ID:SGTC_761|Compound:k216-0224|FD-Score:3.26|P-value:1.58E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:3.65|P-value:2.86E-5 ID:SGTC_831|Compound:k061-1017|FD-Score:4.20|P-value:1.79E-6 ID:SGTC_947|Compound:3447-0010|FD-Score:-2.89|P-value:7.26E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:5.67|P-value:1.91E-10 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.86|P-value:4.19E-8 ID:SGTC_1231|Compound:0242-0792|FD-Score:-2.82|P-value:9.46E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:3.16|P-value:2.47E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:2.81|P-value:9.52E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.12|P-value:2.88E-4 ID:SGTC_1563|Compound:conessine|FD-Score:2.89|P-value:7.05E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.03|P-value:4.08E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:2.82|P-value:9.31E-4 ID:SGTC_1571|Compound:st070080|FD-Score:-2.88|P-value:7.60E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-2.82|P-value:9.30E-4 ID:SGTC_1770|Compound:st043884|FD-Score:-2.98|P-value:5.16E-4 ID:SGTC_1889|Compound:st059080|FD-Score:2.98|P-value:4.98E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:2.85|P-value:8.13E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.24|P-value:1.80E-4 ID:SGTC_2019|Compound:4016004|FD-Score:5.07|P-value:1.06E-8 ID:SGTC_2021|Compound:4019055|FD-Score:4.16|P-value:2.20E-6 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.88|P-value:9.19E-6 ID:SGTC_2316|Compound:5844804|FD-Score:4.14|P-value:2.46E-6 ID:SGTC_2325|Compound:6158266|FD-Score:-3.54|P-value:4.64E-5 ID:SGTC_2514|Compound:salinomycin|FD-Score:2.95|P-value:5.71E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-5.10|P-value:9.41E-9 ID:SGTC_2600|Compound:tangeritin|FD-Score:-4.22|P-value:1.67E-6 ID:SGTC_2693|Compound:st077581|FD-Score:2.97|P-value:5.19E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:2.81|P-value:9.50E-4 ID:SGTC_2803|Compound:7707263|FD-Score:-2.98|P-value:5.12E-4 ID:SGTC_2831|Compound:7999036|FD-Score:2.89|P-value:7.16E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.65|P-value:2.81E-5 ID:SGTC_3224|Compound:9130643|FD-Score:3.02|P-value:4.26E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-2.96|P-value:5.41E-4

Top fitness defect scores for YBR084W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_996 3970-0799 5.67 1.8 µM 1.91E-10 43 7.06E-9
2 SGTC_2019 4016004 5.07 108.0 µM 1.06E-8 6 1.94E-7
3 SGTC_236 1509-0011 4.22 5.3 µM 1.57E-6 367 1.21E-5
4 SGTC_831 k061-1017 4.20 217.0 µM 1.79E-6 translation 51 1.34E-5
5 SGTC_2021 4019055 4.16 173.0 µM 2.20E-6 endomembrane recycling 109 1.60E-5
6 SGTC_2316 5844804 4.14 197.4 µM 2.46E-6 24 1.75E-5
7 SGTC_2247 cerulenin 3.88 830.0 nM 9.19E-6 19 5.22E-5
8 SGTC_669 0108-0021 3.85 26.2 µM 1.05E-5 fatty acid desaturase (OLE1) 87 5.86E-5
9 SGTC_2909 7941780 3.65 10.5 µM 2.81E-5 26 1.32E-4
10 SGTC_762 1165-0429 3.65 115.0 µM 2.86E-5 TSC3-RPN4 26 1.34E-4
11 SGTC_761 k216-0224 3.26 480.0 µM 1.58E-4 50 5.55E-4
12 SGTC_1311 1190-0148 3.16 100.0 µM 2.47E-4 azole & statin 56 8.02E-4
13 SGTC_1376 2910-0861 3.12 41.3 µM 2.88E-4 58 9.11E-4
14 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 3.03 85.4 µM 4.08E-4 69 0.00122
15 SGTC_3224 9130643 3.02 49.5 µM 4.26E-4 RPP1 & pyrimidine depletion 70 0.00126
16 SGTC_1889 st059080 2.98 5.2 µM 4.98E-4 60 0.00144
17 SGTC_2693 st077581 2.97 40.7 µM 5.19E-4 94 0.00149
18 SGTC_2514 salinomycin 2.95 42.7 µM 5.71E-4 105 0.00161
19 SGTC_1563 conessine 2.89 56.1 µM 7.05E-4 64 0.00192
20 SGTC_2831 7999036 2.89 19.5 µM 7.16E-4 120 0.00194

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.337 8.71E-90 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.297 1.60E-69 YDR339C FCF1 het Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
0.293 2.63E-67 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.282 2.70E-62 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.242 8.00E-46 YCL076W_d YCL076W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.238 1.57E-44 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.235 1.94E-43 YGL205W POX1 hom Fatty-acyl coenzyme A oxidase, involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix
0.229 3.08E-41 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.223 6.45E-39 YGL096W TOS8 hom Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
0.222 1.32E-38 YML084W_d YML084W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.219 9.07E-38 YML081C-A ATP18 hom Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms
0.209 1.70E-34 YDR122W KIN1 hom Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p
0.206 1.50E-33 YBR083W TEC1 hom Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member
0.204 8.51E-33 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.204 8.45E-33 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress