YBR278W / DPB3

Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR278W / DPB3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:5.36|P-value:7.71E-8 ID:SGTC_138|Compound:0850-0259|FD-Score:3.24|P-value:7.60E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.29|P-value:5.94E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.83|P-value:1.11E-6 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.82|P-value:9.33E-5 ID:SGTC_264|Compound:promethazine|FD-Score:3.69|P-value:1.56E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.27|P-value:7.02E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:4.76|P-value:1.60E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.98|P-value:2.07E-9 ID:SGTC_552|Compound:k242-0005|FD-Score:5.00|P-value:4.78E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.15|P-value:9.75E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.25|P-value:7.30E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:4.65|P-value:2.68E-6 ID:SGTC_951|Compound:1171-0579|FD-Score:3.58|P-value:2.31E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:4.07|P-value:3.44E-5 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.03|P-value:4.02E-5 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.20|P-value:8.23E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:4.70|P-value:2.08E-6 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.60|P-value:2.14E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.53|P-value:2.78E-4 ID:SGTC_1591|Compound:genipin|FD-Score:4.81|P-value:1.26E-6 ID:SGTC_1764|Compound:st044502|FD-Score:5.54|P-value:2.89E-8 ID:SGTC_1838|Compound:st055369|FD-Score:5.19|P-value:1.88E-7 ID:SGTC_1870|Compound:st057514|FD-Score:3.57|P-value:2.35E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:4.02|P-value:4.10E-5 ID:SGTC_1927|Compound:st060202|FD-Score:-3.75|P-value:1.12E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.26|P-value:6.67E-4 ID:SGTC_1931|Compound:st074438|FD-Score:3.57|P-value:2.43E-4 ID:SGTC_2019|Compound:4016004|FD-Score:4.26|P-value:1.54E-5 ID:SGTC_2104|Compound:5389443|FD-Score:4.01|P-value:4.30E-5 ID:SGTC_2125|Compound:5246583|FD-Score:-3.67|P-value:1.54E-4 ID:SGTC_2196|Compound:6573537|FD-Score:-3.81|P-value:8.92E-5 ID:SGTC_2264|Compound:7948757|FD-Score:-3.57|P-value:2.22E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.29|P-value:6.52E-4 ID:SGTC_2271|Compound:7849410|FD-Score:-6.00|P-value:1.84E-9 ID:SGTC_2272|Compound:7805186|FD-Score:-4.19|P-value:1.83E-5 ID:SGTC_2285|Compound:7945142|FD-Score:-3.73|P-value:1.21E-4 ID:SGTC_2290|Compound:7904846|FD-Score:3.71|P-value:1.39E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.42|P-value:4.09E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:3.94|P-value:5.77E-5 ID:SGTC_2513|Compound:baicalein|FD-Score:6.99|P-value:3.62E-12 ID:SGTC_2532|Compound:xanthone|FD-Score:3.58|P-value:2.31E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.85|P-value:8.32E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:4.71|P-value:2.00E-6 ID:SGTC_2757|Compound:clomiphene|FD-Score:-4.03|P-value:3.70E-5 ID:SGTC_2835|Compound:9006588|FD-Score:5.03|P-value:4.20E-7 ID:SGTC_2853|Compound:9015042|FD-Score:3.54|P-value:2.64E-4 ID:SGTC_3101|Compound:9120531|FD-Score:-3.42|P-value:3.85E-4 ID:SGTC_3264|Compound:9137442|FD-Score:-3.28|P-value:6.10E-4 ID:SGTC_3273|Compound:9137859|FD-Score:3.25|P-value:7.39E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.36|P-value:7.71E-8 ID:SGTC_138|Compound:0850-0259|FD-Score:3.24|P-value:7.60E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.29|P-value:5.94E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.83|P-value:1.11E-6 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.82|P-value:9.33E-5 ID:SGTC_264|Compound:promethazine|FD-Score:3.69|P-value:1.56E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.27|P-value:7.02E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:4.76|P-value:1.60E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.98|P-value:2.07E-9 ID:SGTC_552|Compound:k242-0005|FD-Score:5.00|P-value:4.78E-7 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.15|P-value:9.75E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:3.25|P-value:7.30E-4 ID:SGTC_803|Compound:4488-1585|FD-Score:4.65|P-value:2.68E-6 ID:SGTC_951|Compound:1171-0579|FD-Score:3.58|P-value:2.31E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:4.07|P-value:3.44E-5 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.03|P-value:4.02E-5 ID:SGTC_1261|Compound:0712-0010|FD-Score:-3.20|P-value:8.23E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:4.70|P-value:2.08E-6 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.60|P-value:2.14E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.53|P-value:2.78E-4 ID:SGTC_1591|Compound:genipin|FD-Score:4.81|P-value:1.26E-6 ID:SGTC_1764|Compound:st044502|FD-Score:5.54|P-value:2.89E-8 ID:SGTC_1838|Compound:st055369|FD-Score:5.19|P-value:1.88E-7 ID:SGTC_1870|Compound:st057514|FD-Score:3.57|P-value:2.35E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:4.02|P-value:4.10E-5 ID:SGTC_1927|Compound:st060202|FD-Score:-3.75|P-value:1.12E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.26|P-value:6.67E-4 ID:SGTC_1931|Compound:st074438|FD-Score:3.57|P-value:2.43E-4 ID:SGTC_2019|Compound:4016004|FD-Score:4.26|P-value:1.54E-5 ID:SGTC_2104|Compound:5389443|FD-Score:4.01|P-value:4.30E-5 ID:SGTC_2125|Compound:5246583|FD-Score:-3.67|P-value:1.54E-4 ID:SGTC_2196|Compound:6573537|FD-Score:-3.81|P-value:8.92E-5 ID:SGTC_2264|Compound:7948757|FD-Score:-3.57|P-value:2.22E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.29|P-value:6.52E-4 ID:SGTC_2271|Compound:7849410|FD-Score:-6.00|P-value:1.84E-9 ID:SGTC_2272|Compound:7805186|FD-Score:-4.19|P-value:1.83E-5 ID:SGTC_2285|Compound:7945142|FD-Score:-3.73|P-value:1.21E-4 ID:SGTC_2290|Compound:7904846|FD-Score:3.71|P-value:1.39E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.42|P-value:4.09E-4 ID:SGTC_2508|Compound:celastrol|FD-Score:3.94|P-value:5.77E-5 ID:SGTC_2513|Compound:baicalein|FD-Score:6.99|P-value:3.62E-12 ID:SGTC_2532|Compound:xanthone|FD-Score:3.58|P-value:2.31E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.85|P-value:8.32E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:4.71|P-value:2.00E-6 ID:SGTC_2757|Compound:clomiphene|FD-Score:-4.03|P-value:3.70E-5 ID:SGTC_2835|Compound:9006588|FD-Score:5.03|P-value:4.20E-7 ID:SGTC_2853|Compound:9015042|FD-Score:3.54|P-value:2.64E-4 ID:SGTC_3101|Compound:9120531|FD-Score:-3.42|P-value:3.85E-4 ID:SGTC_3264|Compound:9137442|FD-Score:-3.28|P-value:6.10E-4 ID:SGTC_3273|Compound:9137859|FD-Score:3.25|P-value:7.39E-4

Top fitness defect scores for YBR278W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2513 baicalein 6.99 25.6 µM 3.62E-12 calcium & mitochondrial duress 6 1.34E-12
2 SGTC_1764 st044502 5.54 93.5 µM 2.89E-8 10 1.52E-8
3 SGTC_58 0957-0393 5.36 9.0 µM 7.71E-8 cell wall 251 4.22E-8
4 SGTC_1838 st055369 5.19 84.5 µM 1.88E-7 7 1.07E-7
5 SGTC_2835 9006588 5.03 71.4 µM 4.20E-7 18 2.45E-7
6 SGTC_552 k242-0005 5.00 476.0 µM 4.78E-7 5 2.81E-7
7 SGTC_219 mitoxanthrone 4.83 164.4 µM 1.11E-6 DNA intercalators 29 6.76E-7
8 SGTC_1591 genipin 4.81 88.4 µM 1.26E-6 mitochondrial processes 2 7.69E-7
9 SGTC_380 3970-0968 4.76 6.0 µM 1.60E-6 6 9.85E-7
10 SGTC_2754 granisetron 4.71 64.0 µM 2.00E-6 20 1.24E-6
11 SGTC_1304 1165-0502 4.70 251.0 µM 2.08E-6 46 1.29E-6
12 SGTC_803 4488-1585 4.65 147.0 µM 2.68E-6 7 1.68E-6
13 SGTC_2019 4016004 4.26 108.0 µM 1.54E-5 23 1.03E-5
14 SGTC_1006 4099-6547 4.07 255.0 µM 3.44E-5 17 2.38E-5
15 SGTC_1258 0671-0043 4.03 57.1 µM 4.02E-5 24 2.80E-5
16 SGTC_1879 gemcitabine 4.02 100.0 µM 4.10E-5 10 2.86E-5
17 SGTC_2104 5389443 4.01 200.0 µM 4.30E-5 13 3.01E-5
18 SGTC_2508 celastrol 3.94 40.5 µM 5.77E-5 PDR1 18 4.08E-5
19 SGTC_2566 crystal violet carbinol 3.85 226.0 nM 8.32E-5 41 5.96E-5
20 SGTC_261 5-fluorouridine 3.82 15.4 µM 9.33E-5 exosome 13 6.72E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.147 1.33E-17 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.142 1.45E-16 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.137 1.82E-15 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.137 2.03E-15 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.129 7.34E-14 YDL025C RTK1 hom Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
0.126 2.59E-13 YML016C PPZ1 hom Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
0.125 4.17E-13 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.124 4.53E-13 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.124 6.69E-13 YIL148W RPL40A hom Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.119 5.25E-12 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.116 1.62E-11 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.111 1.13E-10 YJL080C SCP160 hom Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
0.110 1.60E-10 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.109 2.26E-10 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.107 4.22E-10 YCR024C-A PMP1 hom Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication