YBR295W / PCA1

Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function

Zygosity: Homozygous strain
fixedexpanded
Profile for YBR295W / PCA1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:3.14|P-value:9.30E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.19|P-value:7.86E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.50|P-value:4.34E-6 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.64|P-value:1.96E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:-3.48|P-value:3.55E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:4.54|P-value:3.62E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.94|P-value:2.23E-9 ID:SGTC_359|Compound:0081-0058|FD-Score:3.37|P-value:4.20E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.61|P-value:1.72E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.69|P-value:1.30E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.20|P-value:7.63E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.26|P-value:6.20E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:3.22|P-value:7.14E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.30|P-value:5.34E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.58|P-value:1.99E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:5.12|P-value:2.08E-7 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.93|P-value:5.00E-5 ID:SGTC_1656|Compound:st012921|FD-Score:-3.59|P-value:2.34E-4 ID:SGTC_1677|Compound:st019204|FD-Score:4.41|P-value:6.38E-6 ID:SGTC_1682|Compound:st012968|FD-Score:4.07|P-value:2.87E-5 ID:SGTC_1713|Compound:st033146|FD-Score:4.57|P-value:3.13E-6 ID:SGTC_1754|Compound:st042065|FD-Score:3.54|P-value:2.28E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.74|P-value:1.08E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.39|P-value:3.87E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.29|P-value:1.09E-5 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.55|P-value:3.40E-6 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.72|P-value:1.14E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.31|P-value:5.25E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.73|P-value:1.10E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.64|P-value:1.57E-4 ID:SGTC_2155|Compound:5570423|FD-Score:5.17|P-value:1.67E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.35|P-value:5.57E-4 ID:SGTC_3047|Compound:9092122|FD-Score:3.71|P-value:1.20E-4 ID:SGTC_3080|Compound:9117754|FD-Score:5.33|P-value:7.09E-8 ID:SGTC_3214|Compound:9129694|FD-Score:3.50|P-value:2.67E-4 ID:SGTC_3312|Compound:9133330|FD-Score:4.35|P-value:8.59E-6 ID:SGTC_16|Compound:erodoxin|FD-Score:3.14|P-value:9.30E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.19|P-value:7.86E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.50|P-value:4.34E-6 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.64|P-value:1.96E-4 ID:SGTC_165|Compound:k038-0023|FD-Score:-3.48|P-value:3.55E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:4.54|P-value:3.62E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.94|P-value:2.23E-9 ID:SGTC_359|Compound:0081-0058|FD-Score:3.37|P-value:4.20E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.61|P-value:1.72E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.69|P-value:1.30E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:3.20|P-value:7.63E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.26|P-value:6.20E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:3.22|P-value:7.14E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.30|P-value:5.34E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.58|P-value:1.99E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:5.12|P-value:2.08E-7 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.93|P-value:5.00E-5 ID:SGTC_1656|Compound:st012921|FD-Score:-3.59|P-value:2.34E-4 ID:SGTC_1677|Compound:st019204|FD-Score:4.41|P-value:6.38E-6 ID:SGTC_1682|Compound:st012968|FD-Score:4.07|P-value:2.87E-5 ID:SGTC_1713|Compound:st033146|FD-Score:4.57|P-value:3.13E-6 ID:SGTC_1754|Compound:st042065|FD-Score:3.54|P-value:2.28E-4 ID:SGTC_1760|Compound:st045453|FD-Score:3.74|P-value:1.08E-4 ID:SGTC_1801|Compound:st050089|FD-Score:3.39|P-value:3.87E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.29|P-value:1.09E-5 ID:SGTC_1877|Compound:wortmannin|FD-Score:4.55|P-value:3.40E-6 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.72|P-value:1.14E-4 ID:SGTC_2038|Compound:4100038|FD-Score:3.31|P-value:5.25E-4 ID:SGTC_2127|Compound:5253358|FD-Score:3.73|P-value:1.10E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.64|P-value:1.57E-4 ID:SGTC_2155|Compound:5570423|FD-Score:5.17|P-value:1.67E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.35|P-value:5.57E-4 ID:SGTC_3047|Compound:9092122|FD-Score:3.71|P-value:1.20E-4 ID:SGTC_3080|Compound:9117754|FD-Score:5.33|P-value:7.09E-8 ID:SGTC_3214|Compound:9129694|FD-Score:3.50|P-value:2.67E-4 ID:SGTC_3312|Compound:9133330|FD-Score:4.35|P-value:8.59E-6

Top fitness defect scores for YBR295W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 5.94 10.3 µM 2.23E-9 NEO1-PIK1 123 1.40E-9
2 SGTC_3080 9117754 5.33 49.5 µM 7.09E-8 5 4.94E-8
3 SGTC_2155 5570423 5.17 76.0 µM 1.67E-7 ergosterol biosynthesis 4 1.20E-7
4 SGTC_1472 k841-0093 5.12 193.0 µM 2.08E-7 4 1.50E-7
5 SGTC_1713 st033146 4.57 12.8 µM 3.13E-6 14 2.44E-6
6 SGTC_1877 wortmannin 4.55 25.0 µM 3.40E-6 76 2.66E-6
7 SGTC_167 3672-0051 4.54 66.2 µM 3.62E-6 30 2.84E-6
8 SGTC_58 0957-0393 4.50 9.0 µM 4.34E-6 cell wall 329 3.42E-6
9 SGTC_1677 st019204 4.41 53.9 µM 6.38E-6 8 5.09E-6
10 SGTC_3312 9133330 4.35 71.4 µM 8.59E-6 2 6.90E-6
11 SGTC_1803 st050068 4.29 52.4 µM 1.09E-5 NEO1-PIK1 157 8.82E-6
12 SGTC_1682 st012968 4.07 530.0 nM 2.87E-5 14 2.38E-5
13 SGTC_1565 sinapyl alcohol 3.93 95.1 µM 5.00E-5 29 4.23E-5
14 SGTC_1760 st045453 3.74 10.4 µM 1.08E-4 NEO1-PIK1 153 9.31E-5
15 SGTC_2127 5253358 3.73 187.9 µM 1.10E-4 30 9.49E-5
16 SGTC_1878 temozolomide 3.72 1.0 mM 1.14E-4 11 9.84E-5
17 SGTC_3047 9092122 3.71 49.5 µM 1.20E-4 26 1.04E-4
18 SGTC_457 grayanotoxin iii 3.69 135.0 µM 1.30E-4 86 1.13E-4
19 SGTC_2130 NSC 7 3.64 2.5 µM 1.57E-4 copper-dependent oxidative stress 38 1.37E-4
20 SGTC_362 0310-0100 3.61 9.0 µM 1.72E-4 RPP1 & pyrimidine depletion 44 1.51E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.248 2.69E-48 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.244 1.66E-46 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.240 5.15E-45 YDR492W IZH1 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.232 4.42E-42 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.215 2.46E-36 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.202 4.80E-32 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.200 1.36E-31 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.200 2.38E-31 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.197 1.12E-30 YML094C-A_d YML094C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
0.191 6.45E-29 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.190 1.29E-28 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.189 2.88E-28 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.184 5.64E-27 YPR092W_d YPR092W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.184 6.98E-27 YGL077C HNM1 hom Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
0.181 5.37E-26 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p