YCL043C / PDI1

Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication

Zygosity: Heterozygous strain
fixedexpanded
Profile for YCL043C / PDI1

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:5.83|P-value:8.11E-9 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.29|P-value:9.34E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:3.45|P-value:3.91E-4 ID:SGTC_493|Compound:zm226600|FD-Score:-3.34|P-value:7.96E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:3.19|P-value:9.15E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:-3.32|P-value:8.41E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:5.69|P-value:1.81E-8 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.31|P-value:8.67E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:-4.44|P-value:1.23E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:3.32|P-value:6.00E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.79|P-value:1.65E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.76|P-value:1.87E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.44|P-value:5.71E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.78|P-value:1.17E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.22|P-value:8.49E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.83|P-value:9.58E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:-3.69|P-value:2.36E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.09|P-value:3.50E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.39|P-value:9.98E-6 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.54|P-value:2.86E-4 ID:SGTC_1952|Compound:st077036|FD-Score:3.89|P-value:7.63E-5 ID:SGTC_1980|Compound:st071811|FD-Score:3.51|P-value:3.15E-4 ID:SGTC_1985|Compound:st071382|FD-Score:3.46|P-value:3.68E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.63|P-value:2.03E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.96|P-value:5.82E-5 ID:SGTC_2152|Compound:5568786|FD-Score:3.24|P-value:7.89E-4 ID:SGTC_2257|Compound:7960413|FD-Score:-3.28|P-value:9.79E-4 ID:SGTC_2332|Compound:7982454|FD-Score:6.82|P-value:2.10E-11 ID:SGTC_2337|Compound:9009472|FD-Score:4.27|P-value:1.67E-5 ID:SGTC_2338|Compound:7999974|FD-Score:4.31|P-value:1.39E-5 ID:SGTC_2377|Compound:9073351|FD-Score:3.47|P-value:3.56E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.92|P-value:6.87E-5 ID:SGTC_2832|Compound:7992762|FD-Score:5.28|P-value:1.57E-7 ID:SGTC_2834|Compound:9001755|FD-Score:-4.67|P-value:4.51E-6 ID:SGTC_2882|Compound:9058044|FD-Score:-3.93|P-value:9.62E-5 ID:SGTC_2893|Compound:9048488|FD-Score:-3.56|P-value:3.80E-4 ID:SGTC_2895|Compound:9054447|FD-Score:3.55|P-value:2.72E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.96|P-value:1.22E-6 ID:SGTC_2988|Compound:9073640|FD-Score:6.28|P-value:5.93E-10 ID:SGTC_2993|Compound:9054257|FD-Score:-4.62|P-value:5.67E-6 ID:SGTC_2994|Compound:9053520|FD-Score:6.28|P-value:6.10E-10 ID:SGTC_3247|Compound:9135149|FD-Score:3.66|P-value:1.85E-4 ID:SGTC_3315|Compound:9136395|FD-Score:3.87|P-value:8.36E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:5.83|P-value:8.11E-9 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.29|P-value:9.34E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:3.45|P-value:3.91E-4 ID:SGTC_493|Compound:zm226600|FD-Score:-3.34|P-value:7.96E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:3.19|P-value:9.15E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:-3.32|P-value:8.41E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:5.69|P-value:1.81E-8 ID:SGTC_612|Compound:0302-0167|FD-Score:-3.31|P-value:8.67E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:-4.44|P-value:1.23E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:3.32|P-value:6.00E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:-3.79|P-value:1.65E-4 ID:SGTC_924|Compound:2046-0044|FD-Score:-3.76|P-value:1.87E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.44|P-value:5.71E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.78|P-value:1.17E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.22|P-value:8.49E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.83|P-value:9.58E-5 ID:SGTC_1238|Compound:0333-0287|FD-Score:-3.69|P-value:2.36E-4 ID:SGTC_1713|Compound:st033146|FD-Score:4.09|P-value:3.50E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.39|P-value:9.98E-6 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.54|P-value:2.86E-4 ID:SGTC_1952|Compound:st077036|FD-Score:3.89|P-value:7.63E-5 ID:SGTC_1980|Compound:st071811|FD-Score:3.51|P-value:3.15E-4 ID:SGTC_1985|Compound:st071382|FD-Score:3.46|P-value:3.68E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.63|P-value:2.03E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.96|P-value:5.82E-5 ID:SGTC_2152|Compound:5568786|FD-Score:3.24|P-value:7.89E-4 ID:SGTC_2257|Compound:7960413|FD-Score:-3.28|P-value:9.79E-4 ID:SGTC_2332|Compound:7982454|FD-Score:6.82|P-value:2.10E-11 ID:SGTC_2337|Compound:9009472|FD-Score:4.27|P-value:1.67E-5 ID:SGTC_2338|Compound:7999974|FD-Score:4.31|P-value:1.39E-5 ID:SGTC_2377|Compound:9073351|FD-Score:3.47|P-value:3.56E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.92|P-value:6.87E-5 ID:SGTC_2832|Compound:7992762|FD-Score:5.28|P-value:1.57E-7 ID:SGTC_2834|Compound:9001755|FD-Score:-4.67|P-value:4.51E-6 ID:SGTC_2882|Compound:9058044|FD-Score:-3.93|P-value:9.62E-5 ID:SGTC_2893|Compound:9048488|FD-Score:-3.56|P-value:3.80E-4 ID:SGTC_2895|Compound:9054447|FD-Score:3.55|P-value:2.72E-4 ID:SGTC_2896|Compound:9052656|FD-Score:-4.96|P-value:1.22E-6 ID:SGTC_2988|Compound:9073640|FD-Score:6.28|P-value:5.93E-10 ID:SGTC_2993|Compound:9054257|FD-Score:-4.62|P-value:5.67E-6 ID:SGTC_2994|Compound:9053520|FD-Score:6.28|P-value:6.10E-10 ID:SGTC_3247|Compound:9135149|FD-Score:3.66|P-value:1.85E-4 ID:SGTC_3315|Compound:9136395|FD-Score:3.87|P-value:8.36E-5

Top fitness defect scores for YCL043C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2332 7982454 6.82 138.9 µM 2.10E-11 1 4.58E-12 1.14
2 SGTC_2988 9073640 6.28 71.4 µM 5.93E-10 2 1.66E-10 0.10
3 SGTC_2994 9053520 6.28 71.4 µM 6.10E-10 RPP1 & pyrimidine depletion 3 1.71E-10 1.35
4 SGTC_40 0327-0103 5.83 19.8 µM 8.11E-9 2 2.75E-9 0.58
5 SGTC_599 1935-0052 5.69 86.5 µM 1.81E-8 1 6.50E-9 0.95
6 SGTC_2832 7992762 5.28 52.0 µM 1.57E-7 3 6.62E-8 0.23
7 SGTC_1745 st081495 4.39 74.0 µM 9.98E-6 RNA processing & uracil transport 23 5.64E-6 0.03
8 SGTC_2338 7999974 4.31 200.0 µM 1.39E-5 5 8.04E-6 0.18
9 SGTC_2337 9009472 4.27 200.0 µM 1.67E-5 5 9.79E-6 0.07
10 SGTC_1713 st033146 4.09 12.8 µM 3.50E-5 8 2.16E-5 0.02
11 SGTC_2146 5545642 3.96 200.0 µM 5.82E-5 1 3.71E-5 0.26
12 SGTC_2796 7949634 3.92 45.5 µM 6.87E-5 8 4.43E-5 0.07
13 SGTC_1952 st077036 3.89 64.1 µM 7.63E-5 15 4.96E-5 0.16
14 SGTC_3315 9136395 3.87 68.5 µM 8.36E-5 9 5.47E-5 0.09
15 SGTC_1236 0330-0013 3.83 258.0 µM 9.58E-5 2 6.32E-5 0.53
16 SGTC_1038 k292-0204 3.78 154.0 µM 1.17E-4 7 7.85E-5 0.36
17 SGTC_3247 9135149 3.66 49.5 µM 1.85E-4 DNA damage response 26 1.27E-4 0.12
18 SGTC_2079 5212404 3.63 121.0 µM 2.03E-4 2 1.41E-4 0.02
19 SGTC_2895 9054447 3.55 58.4 µM 2.72E-4 2 1.92E-4 0.36
20 SGTC_1928 4'-methoxychalcone 3.54 40.3 µM 2.86E-4 9 2.03E-4 0.62

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.112 8.11E-11 YDR353W TRR1 het Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
0.110 1.98E-10 YML069W POB3 het Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
0.108 3.02E-10 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.103 2.09E-9 YPL099C AIM43 hom Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
0.103 2.40E-9 YBR055C PRP6 het Splicing factor, component of the U4/U6-U5 snRNP complex
0.099 1.02E-8 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.097 2.02E-8 YBR189W RPS9B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.094 5.30E-8 YNR028W CPR8 hom Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway
0.093 6.29E-8 YGR050C_p YGR050C_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data
0.093 6.58E-8 YER168C CCA1 het ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites
0.092 8.35E-8 YJL076W NET1 het Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
0.084 1.19E-6 YMR117C SPC24 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
0.082 1.82E-6 YGR169C PUS6 hom tRNA:pseudouridine synthase, catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability
0.081 2.68E-6 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.078 6.00E-6 YPR096C YPR096C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments