HIPHOP chemogenomics database

YCR094W / CDC50

Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication

Zygosity: Homozygous strain

fixedexpanded
Profile for YCR094W / CDC50

Click on Significant Values for Screen Details

Top fitness defect scores for YCR094W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_637	1181-0519	25.70	7.3 µM	7.34E-99	fatty acid desaturase (OLE1)	2	5.75E-146
2	SGTC_2910	7948405	22.50	26.0 µM	3.71E-76	plasma membrane duress	1	3.03E-112
3	SGTC_137	0438-0332	22.40	85.4 µM	1.40E-75	ubiquinone biosynthesis & proteasome	3	2.17E-111
4	SGTC_1130	3454-2858	21.40	15.9 µM	3.02E-69	NEO1	4	5.56E-102
5	SGTC_1884	5633444	18.00	25.0 µM	1.84E-49	redox potentiating	3	1.34E-72
6	SGTC_439	amiodarone	16.50	34.4 µM	3.56E-42	NEO1-PIK1	3	8.77E-62
7	SGTC_2619	obliquin	15.80	87.9 µM	1.67E-38	cell wall	8	2.47E-56
8	SGTC_887	0885-0007	15.30	9.6 µM	2.91E-36	endomembrane recycling	3	5.25E-53
9	SGTC_788	1182-0090	15.30	188.0 µM	3.32E-36	endomembrane recycling	6	6.37E-53
10	SGTC_2938	9059746	14.50	27.3 µM	6.68E-33	fatty acid desaturase (OLE1)	2	5.10E-48
11	SGTC_181	1447-1644	13.40	8.1 µM	2.41E-28	endomembrane recycling	4	2.95E-41
12	SGTC_2887	9044784	13.30	58.4 µM	7.72E-28	fatty acid desaturase (OLE1)	3	1.66E-40
13	SGTC_1873	rapamycin	12.80	2.0 nM	6.41E-26		3	1.17E-37
14	SGTC_2790	5423970	12.60	71.4 µM	2.73E-25	endomembrane recycling	4	1.01E-36
15	SGTC_2965	9082772	12.50	38.6 µM	8.88E-25	mitochondrial response to ROS	1	5.77E-36
16	SGTC_172	1112-0046	12.40	2.6 µM	2.27E-24	NEO1	3	2.32E-35
17	SGTC_1672	st016598	12.20	6.5 µM	1.08E-23	endomembrane recycling	4	2.35E-34
18	SGTC_1353	1498-1206	12.10	8.1 µM	1.33E-23	mitochondrial response to ROS	1	3.19E-34
19	SGTC_142	0335-0849	12.10	62.7 µM	1.56E-23	ubiquinone biosynthesis & proteasome	22	4.06E-34
20	SGTC_3018	9082602	11.90	49.5 µM	1.18E-22		8	8.11E-33

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.715	0	YJL204C	RCY1	hom	F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
0.509	1.58E-220	YER031C	YPT31	hom	Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
0.492	1.53E-203	YDL226C	GCS1	hom	ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
0.446	1.41E-163	YAL026C	DRS2	hom	Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation and endocytosis; mutations in human homolog ATP8B1 result in liver disease
0.415	1.38E-139	YJL207C	LAA1	hom	AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
0.396	3.81E-126	YOR039W	CKB2	hom	Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.382	5.68E-117	YGL019W	CKB1	hom	Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.371	6.40E-110	YJR060W	CBF1	hom	Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
0.346	4.97E-95	YOR070C	GYP1	hom	Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
0.340	1.29E-91	YLR025W	SNF7	hom	One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.328	1.43E-84	YMR071C	TVP18	hom	Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.322	7.38E-82	YFL034W_p	YFL034W_p	hom	Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.316	3.30E-78	YDL090C	RAM1	hom	Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
0.303	3.38E-72	YGL055W	OLE1	het	Delta(9) fatty acid desaturase, required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
0.298	1.19E-69	YML037C_p	YML037C_p	hom	Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene