YDL084W / SUB2

Component of the TREX complex required for nuclear mRNA export; member of the DEAD-box RNA helicase superfamily and is involved in early and late steps of spliceosome assembly; homolog of the human splicing factor hUAP56; relocalizes from nucleus to cytoplasm upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL084W / SUB2

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:4.84|P-value:2.46E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:3.06|P-value:7.06E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.06|P-value:7.13E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.34|P-value:2.55E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:4.25|P-value:4.91E-6 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.77|P-value:4.48E-5 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.87|P-value:2.88E-5 ID:SGTC_315|Compound:k915-0449|FD-Score:3.24|P-value:3.75E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:-3.07|P-value:8.56E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.59|P-value:9.45E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.14|P-value:6.64E-8 ID:SGTC_499|Compound:genistein|FD-Score:3.90|P-value:2.52E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:9.81|P-value:1.44E-24 ID:SGTC_515|Compound:4092-1064|FD-Score:3.75|P-value:4.70E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-4.44|P-value:2.57E-6 ID:SGTC_520|Compound:3584-0023|FD-Score:-5.70|P-value:2.33E-9 ID:SGTC_759|Compound:3486-0358|FD-Score:3.47|P-value:1.50E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:3.66|P-value:6.90E-5 ID:SGTC_762|Compound:1165-0429|FD-Score:3.10|P-value:6.14E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.35|P-value:2.46E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:3.94|P-value:2.08E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:3.30|P-value:2.94E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:3.16|P-value:5.00E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:3.76|P-value:4.53E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.35|P-value:3.07E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:-3.14|P-value:6.52E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:3.28|P-value:3.19E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:-3.27|P-value:4.06E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:5.22|P-value:3.02E-8 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.21|P-value:4.13E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.31|P-value:3.80E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.23|P-value:3.85E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.88|P-value:2.69E-5 ID:SGTC_1182|Compound:1222-0449|FD-Score:4.29|P-value:4.20E-6 ID:SGTC_1401|Compound:3807-4679|FD-Score:3.56|P-value:1.04E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.80|P-value:3.90E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.84|P-value:4.22E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.63|P-value:8.08E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.69|P-value:6.27E-5 ID:SGTC_1856|Compound:st057054|FD-Score:3.13|P-value:5.45E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.30|P-value:2.95E-4 ID:SGTC_1922|Compound:5578187|FD-Score:3.17|P-value:4.73E-4 ID:SGTC_2012|Compound:4023501|FD-Score:3.65|P-value:7.28E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-5.31|P-value:2.49E-8 ID:SGTC_2057|Compound:5236571|FD-Score:-4.76|P-value:5.12E-7 ID:SGTC_2060|Compound:5238662|FD-Score:3.52|P-value:1.27E-4 ID:SGTC_2108|Compound:5365811|FD-Score:-3.03|P-value:9.72E-4 ID:SGTC_2202|Compound:7226349|FD-Score:3.49|P-value:1.37E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.75|P-value:4.68E-5 ID:SGTC_2459|Compound:5811157|FD-Score:3.00|P-value:8.71E-4 ID:SGTC_2471|Compound:5466956|FD-Score:3.29|P-value:3.06E-4 ID:SGTC_2537|Compound:larixol|FD-Score:5.89|P-value:4.95E-10 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.65|P-value:7.18E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.71|P-value:4.85E-7 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.09|P-value:7.76E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.34|P-value:3.09E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.83|P-value:3.37E-5 ID:SGTC_2807|Compound:7945238|FD-Score:-3.07|P-value:8.31E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-3.34|P-value:3.11E-4 ID:SGTC_2864|Compound:9034755|FD-Score:-3.03|P-value:9.61E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.27|P-value:3.35E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-4.44|P-value:2.57E-6 ID:SGTC_3014|Compound:9081533|FD-Score:3.23|P-value:3.78E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-5.24|P-value:3.62E-8 ID:SGTC_3327|Compound:9140648|FD-Score:3.86|P-value:2.91E-5 ID:SGTC_3337|Compound:9144342|FD-Score:5.94|P-value:3.65E-10 ID:SGTC_57|Compound:miconazole|FD-Score:4.84|P-value:2.46E-7 ID:SGTC_70|Compound:0141-0289|FD-Score:3.06|P-value:7.06E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.06|P-value:7.13E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.34|P-value:2.55E-4 ID:SGTC_208|Compound:k072-0098|FD-Score:4.25|P-value:4.91E-6 ID:SGTC_254|Compound:nsc-64875|FD-Score:3.77|P-value:4.48E-5 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.87|P-value:2.88E-5 ID:SGTC_315|Compound:k915-0449|FD-Score:3.24|P-value:3.75E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:-3.07|P-value:8.56E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.59|P-value:9.45E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.14|P-value:6.64E-8 ID:SGTC_499|Compound:genistein|FD-Score:3.90|P-value:2.52E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:9.81|P-value:1.44E-24 ID:SGTC_515|Compound:4092-1064|FD-Score:3.75|P-value:4.70E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-4.44|P-value:2.57E-6 ID:SGTC_520|Compound:3584-0023|FD-Score:-5.70|P-value:2.33E-9 ID:SGTC_759|Compound:3486-0358|FD-Score:3.47|P-value:1.50E-4 ID:SGTC_761|Compound:k216-0224|FD-Score:3.66|P-value:6.90E-5 ID:SGTC_762|Compound:1165-0429|FD-Score:3.10|P-value:6.14E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.35|P-value:2.46E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:3.94|P-value:2.08E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:3.30|P-value:2.94E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:3.16|P-value:5.00E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:3.76|P-value:4.53E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.35|P-value:3.07E-4 ID:SGTC_961|Compound:1252-1571|FD-Score:-3.14|P-value:6.52E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:3.28|P-value:3.19E-4 ID:SGTC_1012|Compound:4112-3522|FD-Score:-3.27|P-value:4.06E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:5.22|P-value:3.02E-8 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.21|P-value:4.13E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.31|P-value:3.80E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.23|P-value:3.85E-4 ID:SGTC_1168|Compound:k815-0004|FD-Score:3.88|P-value:2.69E-5 ID:SGTC_1182|Compound:1222-0449|FD-Score:4.29|P-value:4.20E-6 ID:SGTC_1401|Compound:3807-4679|FD-Score:3.56|P-value:1.04E-4 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.80|P-value:3.90E-5 ID:SGTC_1676|Compound:st018489|FD-Score:-3.84|P-value:4.22E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.63|P-value:8.08E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.69|P-value:6.27E-5 ID:SGTC_1856|Compound:st057054|FD-Score:3.13|P-value:5.45E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.30|P-value:2.95E-4 ID:SGTC_1922|Compound:5578187|FD-Score:3.17|P-value:4.73E-4 ID:SGTC_2012|Compound:4023501|FD-Score:3.65|P-value:7.28E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-5.31|P-value:2.49E-8 ID:SGTC_2057|Compound:5236571|FD-Score:-4.76|P-value:5.12E-7 ID:SGTC_2060|Compound:5238662|FD-Score:3.52|P-value:1.27E-4 ID:SGTC_2108|Compound:5365811|FD-Score:-3.03|P-value:9.72E-4 ID:SGTC_2202|Compound:7226349|FD-Score:3.49|P-value:1.37E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.75|P-value:4.68E-5 ID:SGTC_2459|Compound:5811157|FD-Score:3.00|P-value:8.71E-4 ID:SGTC_2471|Compound:5466956|FD-Score:3.29|P-value:3.06E-4 ID:SGTC_2537|Compound:larixol|FD-Score:5.89|P-value:4.95E-10 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.65|P-value:7.18E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.71|P-value:4.85E-7 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.09|P-value:7.76E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.34|P-value:3.09E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.83|P-value:3.37E-5 ID:SGTC_2807|Compound:7945238|FD-Score:-3.07|P-value:8.31E-4 ID:SGTC_2835|Compound:9006588|FD-Score:-3.34|P-value:3.11E-4 ID:SGTC_2864|Compound:9034755|FD-Score:-3.03|P-value:9.61E-4 ID:SGTC_2925|Compound:9010536|FD-Score:3.27|P-value:3.35E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-4.44|P-value:2.57E-6 ID:SGTC_3014|Compound:9081533|FD-Score:3.23|P-value:3.78E-4 ID:SGTC_3203|Compound:9113543|FD-Score:-5.24|P-value:3.62E-8 ID:SGTC_3327|Compound:9140648|FD-Score:3.86|P-value:2.91E-5 ID:SGTC_3337|Compound:9144342|FD-Score:5.94|P-value:3.65E-10

Top fitness defect scores for YDL084W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_514 4466-0038 9.81 2.6 nM 1.44E-24 cell wall 3 4.85E-23 0.87
2 SGTC_3337 9144342 5.94 33.5 µM 3.65E-10 8 1.45E-9 1.40
3 SGTC_2537 larixol 5.89 68.9 µM 4.95E-10 2 1.93E-9 0.60
4 SGTC_1036 k216-0215 5.22 33.5 µM 3.02E-8 mitochondrial response to ROS 2 9.04E-8 1.00
5 SGTC_57 miconazole 4.84 162.5 nM 2.46E-7 ergosterol depletion effects on membrane 19 6.42E-7 0.12
6 SGTC_2567 aureobasidin a 4.71 60.0 nM 4.85E-7 32 1.21E-6 0.09
7 SGTC_1072 oxiconazole 4.31 14.9 nM 3.80E-6 ergosterol depletion effects on membrane 16 8.30E-6 0.05
8 SGTC_1182 1222-0449 4.29 220.0 µM 4.20E-6 2 9.11E-6 0.50
9 SGTC_208 k072-0098 4.25 12.4 µM 4.91E-6 heme biosynthesis & mitochondrial translocase 16 1.05E-5 0.10
10 SGTC_786 4544-0069 3.94 423.0 µM 2.08E-5 8 4.06E-5 0.24
11 SGTC_499 genistein 3.90 192.0 µM 2.52E-5 2 4.87E-5 0.57
12 SGTC_1168 k815-0004 3.88 80.5 µM 2.69E-5 DNA damage response 14 5.17E-5 0.24
13 SGTC_256 clotrimazole 3.87 625.0 nM 2.88E-5 ergosterol depletion effects on membrane 23 5.50E-5 0.08
14 SGTC_3327 9140648 3.86 26.9 µM 2.91E-5 15 5.56E-5 0.02
15 SGTC_2778 tioconazole 3.83 65.1 nM 3.37E-5 ergosterol depletion effects on membrane 32 6.39E-5 0.04
16 SGTC_1453 4237-0541 3.80 195.0 µM 3.90E-5 heme biosynthesis & mitochondrial translocase 17 7.31E-5 0.05
17 SGTC_254 nsc-64875 3.77 3.8 µM 4.48E-5 ergosterol depletion effects on membrane 4 8.32E-5 0.28
18 SGTC_876 0720-0963 3.76 56.8 µM 4.53E-5 7 8.41E-5 0.09
19 SGTC_2305 7443522 3.75 69.2 µM 4.68E-5 10 8.68E-5 0.23
20 SGTC_515 4092-1064 3.75 102.0 µM 4.70E-5 plasma membrane duress 21 8.70E-5 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.173 6.16E-24 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.167 1.70E-22 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.162 4.51E-21 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.147 9.15E-18 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.140 4.38E-16 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.139 7.57E-16 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.135 4.24E-15 YMR106C YKU80 hom Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
0.134 5.51E-15 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.129 6.89E-14 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.127 1.54E-13 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.126 2.42E-13 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.126 2.92E-13 YER008C SEC3 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.123 1.00E-12 YOR203W_d YOR203W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps 5' end of essential DED1 gene required for translation initiation
0.121 1.83E-12 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.121 1.84E-12 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6