YDL101C / DUN1

Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL101C / DUN1

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:3.39|P-value:7.05E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:4.09|P-value:6.03E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:3.34|P-value:8.40E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.79|P-value:3.25E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:3.62|P-value:3.34E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.28|P-value:9.96E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.03|P-value:7.56E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:4.61|P-value:7.07E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:6.02|P-value:7.58E-9 ID:SGTC_514|Compound:4466-0038|FD-Score:3.99|P-value:8.76E-5 ID:SGTC_558|Compound:k064-0027|FD-Score:4.14|P-value:4.88E-5 ID:SGTC_599|Compound:1935-0052|FD-Score:4.54|P-value:9.79E-6 ID:SGTC_617|Compound:3770-0098|FD-Score:-3.67|P-value:2.91E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:3.69|P-value:2.58E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:3.95|P-value:1.02E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:4.39|P-value:1.80E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:-3.52|P-value:4.70E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.71|P-value:2.48E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:4.42|P-value:1.62E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:3.90|P-value:1.23E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:-3.49|P-value:5.36E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.74|P-value:2.18E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.58|P-value:3.74E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:4.68|P-value:5.26E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.07|P-value:6.30E-5 ID:SGTC_1253|Compound:0550-0034|FD-Score:4.01|P-value:7.92E-5 ID:SGTC_1399|Compound:3562-5772|FD-Score:3.63|P-value:3.19E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:3.72|P-value:2.34E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:3.33|P-value:8.56E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:4.27|P-value:2.92E-5 ID:SGTC_1471|Compound:k673-0312|FD-Score:4.13|P-value:5.03E-5 ID:SGTC_1733|Compound:st037357|FD-Score:3.55|P-value:4.18E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:4.47|P-value:1.33E-5 ID:SGTC_1915|Compound:st067105|FD-Score:5.51|P-value:1.12E-7 ID:SGTC_1959|Compound:st077091|FD-Score:3.59|P-value:3.70E-4 ID:SGTC_2003|Compound:4023581|FD-Score:12.10|P-value:2.45E-30 ID:SGTC_2004|Compound:4023728|FD-Score:8.69|P-value:1.47E-16 ID:SGTC_2007|Compound:4011539|FD-Score:-3.62|P-value:3.38E-4 ID:SGTC_2008|Compound:4012253|FD-Score:4.20|P-value:3.83E-5 ID:SGTC_2009|Compound:4012297|FD-Score:-12.30|P-value:2.03E-31 ID:SGTC_2030|Compound:5175110|FD-Score:3.67|P-value:2.80E-4 ID:SGTC_2046|Compound:4110841|FD-Score:3.29|P-value:9.85E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.52|P-value:4.56E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.32|P-value:9.01E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.50|P-value:4.94E-4 ID:SGTC_2382|Compound:9035899|FD-Score:-4.99|P-value:1.39E-6 ID:SGTC_2429|Compound:5902844|FD-Score:4.50|P-value:1.17E-5 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.04|P-value:7.19E-5 ID:SGTC_2556|Compound:citropten|FD-Score:3.37|P-value:7.67E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:5.18|P-value:5.59E-7 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.38|P-value:1.89E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:7.02|P-value:2.01E-11 ID:SGTC_2817|Compound:7995342|FD-Score:3.41|P-value:6.76E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.99|P-value:8.78E-5 ID:SGTC_2834|Compound:9001755|FD-Score:-3.41|P-value:6.77E-4 ID:SGTC_2996|Compound:9066192|FD-Score:4.54|P-value:9.83E-6 ID:SGTC_3001|Compound:9076041|FD-Score:3.41|P-value:6.68E-4 ID:SGTC_3088|Compound:9117578|FD-Score:4.43|P-value:1.55E-5 ID:SGTC_3174|Compound:9107204|FD-Score:3.98|P-value:8.99E-5 ID:SGTC_3223|Compound:9129970|FD-Score:3.40|P-value:6.80E-4 ID:SGTC_3247|Compound:9135149|FD-Score:5.75|P-value:3.24E-8 ID:SGTC_8|Compound:0354-0001|FD-Score:3.39|P-value:7.05E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:4.09|P-value:6.03E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:3.34|P-value:8.40E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.79|P-value:3.25E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:3.62|P-value:3.34E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.28|P-value:9.96E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.03|P-value:7.56E-5 ID:SGTC_268|Compound:camptothecin|FD-Score:4.61|P-value:7.07E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:6.02|P-value:7.58E-9 ID:SGTC_514|Compound:4466-0038|FD-Score:3.99|P-value:8.76E-5 ID:SGTC_558|Compound:k064-0027|FD-Score:4.14|P-value:4.88E-5 ID:SGTC_599|Compound:1935-0052|FD-Score:4.54|P-value:9.79E-6 ID:SGTC_617|Compound:3770-0098|FD-Score:-3.67|P-value:2.91E-4 ID:SGTC_624|Compound:k048-0052|FD-Score:3.69|P-value:2.58E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:3.95|P-value:1.02E-4 ID:SGTC_744|Compound:1594-0068|FD-Score:4.39|P-value:1.80E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:-3.52|P-value:4.70E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.71|P-value:2.48E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:4.42|P-value:1.62E-5 ID:SGTC_829|Compound:1635-0120|FD-Score:3.90|P-value:1.23E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:-3.49|P-value:5.36E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:3.74|P-value:2.18E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.58|P-value:3.74E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:4.68|P-value:5.26E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.07|P-value:6.30E-5 ID:SGTC_1253|Compound:0550-0034|FD-Score:4.01|P-value:7.92E-5 ID:SGTC_1399|Compound:3562-5772|FD-Score:3.63|P-value:3.19E-4 ID:SGTC_1461|Compound:k072-0203|FD-Score:3.72|P-value:2.34E-4 ID:SGTC_1466|Compound:k213-0079|FD-Score:3.33|P-value:8.56E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:4.27|P-value:2.92E-5 ID:SGTC_1471|Compound:k673-0312|FD-Score:4.13|P-value:5.03E-5 ID:SGTC_1733|Compound:st037357|FD-Score:3.55|P-value:4.18E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:4.47|P-value:1.33E-5 ID:SGTC_1915|Compound:st067105|FD-Score:5.51|P-value:1.12E-7 ID:SGTC_1959|Compound:st077091|FD-Score:3.59|P-value:3.70E-4 ID:SGTC_2003|Compound:4023581|FD-Score:12.10|P-value:2.45E-30 ID:SGTC_2004|Compound:4023728|FD-Score:8.69|P-value:1.47E-16 ID:SGTC_2007|Compound:4011539|FD-Score:-3.62|P-value:3.38E-4 ID:SGTC_2008|Compound:4012253|FD-Score:4.20|P-value:3.83E-5 ID:SGTC_2009|Compound:4012297|FD-Score:-12.30|P-value:2.03E-31 ID:SGTC_2030|Compound:5175110|FD-Score:3.67|P-value:2.80E-4 ID:SGTC_2046|Compound:4110841|FD-Score:3.29|P-value:9.85E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.52|P-value:4.56E-4 ID:SGTC_2289|Compound:7946404|FD-Score:3.32|P-value:9.01E-4 ID:SGTC_2381|Compound:9035592|FD-Score:3.50|P-value:4.94E-4 ID:SGTC_2382|Compound:9035899|FD-Score:-4.99|P-value:1.39E-6 ID:SGTC_2429|Compound:5902844|FD-Score:4.50|P-value:1.17E-5 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.04|P-value:7.19E-5 ID:SGTC_2556|Compound:citropten|FD-Score:3.37|P-value:7.67E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:5.18|P-value:5.59E-7 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.38|P-value:1.89E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:7.02|P-value:2.01E-11 ID:SGTC_2817|Compound:7995342|FD-Score:3.41|P-value:6.76E-4 ID:SGTC_2830|Compound:7992007|FD-Score:3.99|P-value:8.78E-5 ID:SGTC_2834|Compound:9001755|FD-Score:-3.41|P-value:6.77E-4 ID:SGTC_2996|Compound:9066192|FD-Score:4.54|P-value:9.83E-6 ID:SGTC_3001|Compound:9076041|FD-Score:3.41|P-value:6.68E-4 ID:SGTC_3088|Compound:9117578|FD-Score:4.43|P-value:1.55E-5 ID:SGTC_3174|Compound:9107204|FD-Score:3.98|P-value:8.99E-5 ID:SGTC_3223|Compound:9129970|FD-Score:3.40|P-value:6.80E-4 ID:SGTC_3247|Compound:9135149|FD-Score:5.75|P-value:3.24E-8

Top fitness defect scores for YDL101C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2003 4023581 12.10 200.0 µM 2.45E-30 cell wall signaling 2 4.67E-34
2 SGTC_2004 4023728 8.69 41.9 µM 1.47E-16 2 1.74E-18
3 SGTC_2619 obliquin 7.02 87.9 µM 2.01E-11 cell wall 58 1.10E-12
4 SGTC_273 hydroxyurea 6.02 18.1 mM 7.58E-9 heme biosynthesis & mitochondrial translocase 10 8.87E-10
5 SGTC_3247 9135149 5.75 49.5 µM 3.24E-8 DNA damage response 13 4.57E-9
6 SGTC_1915 st067105 5.51 30.6 µM 1.12E-7 RNA pol III & RNase P/MRP 9 1.84E-8
7 SGTC_2584 androsterone 5.18 48.7 µM 5.59E-7 cell wall 76 1.13E-7
8 SGTC_70 0141-0289 4.79 43.3 µM 3.25E-6 mitochondrial stress 120 8.20E-7
9 SGTC_1153 4055-0057 4.68 267.0 µM 5.26E-6 8 1.41E-6
10 SGTC_268 camptothecin 4.61 39.6 µM 7.07E-6 DNA damage response 54 1.97E-6
11 SGTC_599 1935-0052 4.54 86.5 µM 9.79E-6 9 2.84E-6
12 SGTC_2996 9066192 4.54 71.4 µM 9.83E-6 19 2.85E-6
13 SGTC_2429 5902844 4.50 64.4 µM 1.17E-5 10 3.47E-6
14 SGTC_1879 gemcitabine 4.47 100.0 µM 1.33E-5 6 4.00E-6
15 SGTC_3088 9117578 4.43 49.5 µM 1.55E-5 amide catabolism 6 4.74E-6
16 SGTC_788 1182-0090 4.42 188.0 µM 1.62E-5 endomembrane recycling 67 5.02E-6
17 SGTC_744 1594-0068 4.39 283.0 µM 1.80E-5 amide catabolism 18 5.62E-6
18 SGTC_2588 indole-3-carbinol 4.38 100.0 µM 1.89E-5 30 5.94E-6
19 SGTC_1467 k284-1082 4.27 293.0 µM 2.92E-5 10 9.69E-6
20 SGTC_2008 4012253 4.20 172.0 µM 3.83E-5 19 1.32E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 1.19E-23 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.157 6.06E-20 YBR202W MCM7 het Component of the heterohexameric MCM2-7 complex, which primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
0.145 2.76E-17 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.139 6.36E-16 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.139 6.54E-16 YLR342W FKS1 hom Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling
0.136 2.76E-15 YJL027C_p YJL027C_p hom Putative protein of unknown function
0.133 1.10E-14 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.124 5.03E-13 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.122 1.27E-12 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.120 3.31E-12 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.120 3.63E-12 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.119 5.51E-12 YNL075W IMP4 het Component of the SSU processome, which is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
0.118 6.55E-12 YPR143W RRP15 het Nucleolar protein, constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
0.118 8.53E-12 YNL112W DBP2 het ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
0.116 1.37E-11 YNL273W TOF1 hom Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase