YDL108W / KIN28

Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL108W / KIN28

Click on Significant Values for Screen Details ID:SGTC_119|Compound:0121-0043|FD-Score:3.89|P-value:2.78E-5 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.21|P-value:4.39E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:7.32|P-value:1.64E-14 ID:SGTC_277|Compound:nystatin|FD-Score:-3.85|P-value:3.34E-5 ID:SGTC_345|Compound:1362-0200|FD-Score:4.18|P-value:7.28E-6 ID:SGTC_349|Compound:k015-0014|FD-Score:3.95|P-value:2.17E-5 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.10|P-value:6.55E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.49|P-value:1.51E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.48|P-value:1.51E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:5.65|P-value:2.41E-9 ID:SGTC_529|Compound:4076-0302|FD-Score:7.59|P-value:1.76E-15 ID:SGTC_530|Compound:4358-1714|FD-Score:3.00|P-value:9.38E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.35|P-value:2.61E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.06|P-value:7.50E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:3.10|P-value:6.65E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.54|P-value:1.20E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.43|P-value:1.92E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:-3.48|P-value:1.54E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.98|P-value:1.86E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:3.44|P-value:1.78E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:5.76|P-value:1.15E-9 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.15|P-value:5.52E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.29|P-value:3.30E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.38|P-value:2.33E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.62|P-value:8.86E-5 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.12|P-value:6.02E-4 ID:SGTC_1638|Compound:st008370|FD-Score:3.60|P-value:9.57E-5 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.21|P-value:4.41E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.17|P-value:5.13E-4 ID:SGTC_2099|Compound:5377090|FD-Score:4.13|P-value:9.42E-6 ID:SGTC_2184|Compound:6075246|FD-Score:3.04|P-value:8.25E-4 ID:SGTC_2218|Compound:6965428|FD-Score:-3.35|P-value:2.55E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.44|P-value:2.05E-6 ID:SGTC_2266|Compound:7929956|FD-Score:3.25|P-value:3.73E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.54|P-value:1.23E-4 ID:SGTC_2277|Compound:7947813|FD-Score:-3.50|P-value:1.44E-4 ID:SGTC_2305|Compound:7443522|FD-Score:7.48|P-value:4.35E-15 ID:SGTC_2307|Compound:7538926|FD-Score:6.26|P-value:4.40E-11 ID:SGTC_2309|Compound:7664088|FD-Score:4.39|P-value:2.72E-6 ID:SGTC_2315|Compound:7733312|FD-Score:-6.27|P-value:4.12E-11 ID:SGTC_2585|Compound:alliin|FD-Score:-3.22|P-value:4.13E-4 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:-2.98|P-value:9.94E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.61|P-value:9.29E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-3.11|P-value:6.28E-4 ID:SGTC_3205|Compound:9113483|FD-Score:4.21|P-value:6.40E-6 ID:SGTC_3206|Compound:9114350|FD-Score:-3.43|P-value:1.90E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-8.81|P-value:3.22E-20 ID:SGTC_119|Compound:0121-0043|FD-Score:3.89|P-value:2.78E-5 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.21|P-value:4.39E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:7.32|P-value:1.64E-14 ID:SGTC_277|Compound:nystatin|FD-Score:-3.85|P-value:3.34E-5 ID:SGTC_345|Compound:1362-0200|FD-Score:4.18|P-value:7.28E-6 ID:SGTC_349|Compound:k015-0014|FD-Score:3.95|P-value:2.17E-5 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.10|P-value:6.55E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:3.49|P-value:1.51E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.48|P-value:1.51E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:5.65|P-value:2.41E-9 ID:SGTC_529|Compound:4076-0302|FD-Score:7.59|P-value:1.76E-15 ID:SGTC_530|Compound:4358-1714|FD-Score:3.00|P-value:9.38E-4 ID:SGTC_536|Compound:3852-0578|FD-Score:3.35|P-value:2.61E-4 ID:SGTC_572|Compound:0960-0087|FD-Score:3.06|P-value:7.50E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:3.10|P-value:6.65E-4 ID:SGTC_686|Compound:1319-0111|FD-Score:-3.54|P-value:1.20E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.43|P-value:1.92E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:-3.48|P-value:1.54E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.98|P-value:1.86E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:3.44|P-value:1.78E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:5.76|P-value:1.15E-9 ID:SGTC_1022|Compound:4477-0945|FD-Score:3.15|P-value:5.52E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.29|P-value:3.30E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.38|P-value:2.33E-4 ID:SGTC_1369|Compound:1866-0067|FD-Score:-3.62|P-value:8.86E-5 ID:SGTC_1448|Compound:4255-0083|FD-Score:3.12|P-value:6.02E-4 ID:SGTC_1638|Compound:st008370|FD-Score:3.60|P-value:9.57E-5 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.21|P-value:4.41E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.17|P-value:5.13E-4 ID:SGTC_2099|Compound:5377090|FD-Score:4.13|P-value:9.42E-6 ID:SGTC_2184|Compound:6075246|FD-Score:3.04|P-value:8.25E-4 ID:SGTC_2218|Compound:6965428|FD-Score:-3.35|P-value:2.55E-4 ID:SGTC_2239|Compound:6685329|FD-Score:4.44|P-value:2.05E-6 ID:SGTC_2266|Compound:7929956|FD-Score:3.25|P-value:3.73E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.54|P-value:1.23E-4 ID:SGTC_2277|Compound:7947813|FD-Score:-3.50|P-value:1.44E-4 ID:SGTC_2305|Compound:7443522|FD-Score:7.48|P-value:4.35E-15 ID:SGTC_2307|Compound:7538926|FD-Score:6.26|P-value:4.40E-11 ID:SGTC_2309|Compound:7664088|FD-Score:4.39|P-value:2.72E-6 ID:SGTC_2315|Compound:7733312|FD-Score:-6.27|P-value:4.12E-11 ID:SGTC_2585|Compound:alliin|FD-Score:-3.22|P-value:4.13E-4 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:-2.98|P-value:9.94E-4 ID:SGTC_2800|Compound:7887935|FD-Score:3.61|P-value:9.29E-5 ID:SGTC_2971|Compound:9092186|FD-Score:-3.11|P-value:6.28E-4 ID:SGTC_3205|Compound:9113483|FD-Score:4.21|P-value:6.40E-6 ID:SGTC_3206|Compound:9114350|FD-Score:-3.43|P-value:1.90E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-8.81|P-value:3.22E-20

Top fitness defect scores for YDL108W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_529 4076-0302 7.59 30.3 µM 1.76E-15 mitochondrial processes 1 1.56E-14 1.78
2 SGTC_2305 7443522 7.48 69.2 µM 4.35E-15 1 3.62E-14 1.61
3 SGTC_168 3970-0862 7.32 530.5 µM 1.64E-14 12 1.25E-13 0.12
4 SGTC_2307 7538926 6.26 6.3 µM 4.40E-11 6 1.96E-10 0.55
5 SGTC_996 3970-0799 5.76 1.8 µM 1.15E-9 9 4.09E-9 0.14
6 SGTC_526 4469-0568 5.65 170.0 µM 2.41E-9 azole & statin 3 8.16E-9 0.37
7 SGTC_2239 6685329 4.44 187.6 µM 2.05E-6 3 4.41E-6 0.28
8 SGTC_2309 7664088 4.39 163.9 µM 2.72E-6 7 5.75E-6 0.26
9 SGTC_3205 9113483 4.21 49.5 µM 6.40E-6 5 1.28E-5 0.16
10 SGTC_345 1362-0200 4.18 395.0 µM 7.28E-6 3 1.44E-5 0.57
11 SGTC_2099 5377090 4.13 177.4 µM 9.42E-6 4 1.83E-5 0.50
12 SGTC_349 k015-0014 3.95 24.0 µM 2.17E-5 7 3.99E-5 0.24
13 SGTC_119 0121-0043 3.89 24.8 µM 2.78E-5 mitochondrial stress 23 5.04E-5 0.02
14 SGTC_2800 7887935 3.61 81.8 µM 9.29E-5 RPP1 & pyrimidine depletion 11 1.55E-4 0.10
15 SGTC_1638 st008370 3.60 60.6 µM 9.57E-5 ergosterol biosynthesis 18 1.60E-4 0.23
16 SGTC_421 streptovitacin 3.49 30.0 mM 1.51E-4 4 2.44E-4 0.17
17 SGTC_942 3253-1115 3.44 227.0 µM 1.78E-4 12 2.86E-4 0.06
18 SGTC_696 1560-0013 3.43 611.0 µM 1.92E-4 15 3.06E-4 0.07
19 SGTC_1239 0337-0584 3.38 35.4 µM 2.33E-4 unfolded protein response 39 3.67E-4 0.07
20 SGTC_536 3852-0578 3.35 10.6 µM 2.61E-4 6 4.09E-4 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.176 1.01E-24 YML118W NGL3 hom 3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
0.160 1.37E-20 YGL222C EDC1 hom RNA-binding protein that activates mRNA decapping directly; binds to the mRNA substrate and enhances the activity of the decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress
0.140 3.91E-16 YDL099W BUG1 hom Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
0.139 7.15E-16 YNR033W ABZ1 hom Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
0.138 1.25E-15 YJR129C_p YJR129C_p hom Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.132 1.49E-14 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.131 2.96E-14 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.128 1.30E-13 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.125 3.78E-13 YJR094C IME1 hom Master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p
0.125 4.26E-13 YDR505C PSP1 hom Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
0.118 7.79E-12 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.115 2.47E-11 YML100W-A_p YML100W-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.115 2.76E-11 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.114 3.44E-11 YDL186W_p YDL186W_p hom Putative protein of unknown function; YDL186W is not an essential gene
0.110 1.97E-10 YDR002W YRB1 het Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1