YDL140C / RPO21

RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL140C / RPO21

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:3.82|P-value:7.68E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:6.64|P-value:2.85E-14 ID:SGTC_171|Compound:k064-0041|FD-Score:2.75|P-value:8.93E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.96|P-value:3.66E-6 ID:SGTC_424|Compound:splitomicin|FD-Score:3.03|P-value:2.95E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:4.37|P-value:3.80E-7 ID:SGTC_522|Compound:1273-0010|FD-Score:3.02|P-value:3.04E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:4.95|P-value:1.04E-8 ID:SGTC_584|Compound:0986-0246|FD-Score:3.03|P-value:3.02E-4 ID:SGTC_603|Compound:3794-0023|FD-Score:3.19|P-value:1.49E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:-2.94|P-value:4.56E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:-2.76|P-value:9.15E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:2.73|P-value:9.78E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:4.66|P-value:6.58E-8 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.04|P-value:2.84E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.92|P-value:4.55E-6 ID:SGTC_1151|Compound:3909-8316|FD-Score:-3.20|P-value:1.53E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:-4.18|P-value:1.20E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:-3.96|P-value:4.03E-6 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.44|P-value:4.79E-5 ID:SGTC_1164|Compound:k060-0068|FD-Score:2.76|P-value:8.72E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:2.95|P-value:4.06E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:-2.78|P-value:8.71E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.29|P-value:1.02E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:4.18|P-value:1.09E-6 ID:SGTC_1337|Compound:1486-1011|FD-Score:2.90|P-value:4.98E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:-2.88|P-value:5.88E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:3.09|P-value:2.33E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-2.80|P-value:7.92E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.09|P-value:2.34E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.82|P-value:7.65E-6 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.41|P-value:5.66E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:2.82|P-value:6.86E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-3.01|P-value:3.52E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.07|P-value:2.47E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-3.95|P-value:4.07E-6 ID:SGTC_1932|Compound:st074806|FD-Score:-2.76|P-value:9.45E-4 ID:SGTC_1936|Compound:st074407|FD-Score:5.72|P-value:4.74E-11 ID:SGTC_2240|Compound:6690706|FD-Score:-2.77|P-value:8.94E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:2.77|P-value:8.35E-4 ID:SGTC_2311|Compound:7694451|FD-Score:2.83|P-value:6.70E-4 ID:SGTC_2348|Compound:9022543|FD-Score:44.20|P-value:0 ID:SGTC_2375|Compound:9072683|FD-Score:2.91|P-value:4.87E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:5.02|P-value:6.45E-9 ID:SGTC_2460|Compound:5552655|FD-Score:3.60|P-value:2.29E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.59|P-value:2.33E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:2.90|P-value:4.98E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-2.90|P-value:5.40E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.78|P-value:9.39E-6 ID:SGTC_2969|Compound:9091408|FD-Score:-2.95|P-value:4.39E-4 ID:SGTC_2970|Compound:9090938|FD-Score:2.75|P-value:9.09E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-2.96|P-value:4.19E-4 ID:SGTC_2995|Compound:9067767|FD-Score:-2.84|P-value:6.73E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.07|P-value:2.55E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-3.05|P-value:2.93E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.12|P-value:2.00E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.21|P-value:1.38E-4 ID:SGTC_3291|Compound:9121177|FD-Score:2.82|P-value:6.96E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.82|P-value:7.68E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:6.64|P-value:2.85E-14 ID:SGTC_171|Compound:k064-0041|FD-Score:2.75|P-value:8.93E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.96|P-value:3.66E-6 ID:SGTC_424|Compound:splitomicin|FD-Score:3.03|P-value:2.95E-4 ID:SGTC_429|Compound:1112-0049|FD-Score:4.37|P-value:3.80E-7 ID:SGTC_522|Compound:1273-0010|FD-Score:3.02|P-value:3.04E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:4.95|P-value:1.04E-8 ID:SGTC_584|Compound:0986-0246|FD-Score:3.03|P-value:3.02E-4 ID:SGTC_603|Compound:3794-0023|FD-Score:3.19|P-value:1.49E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:-2.94|P-value:4.56E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:-2.76|P-value:9.15E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:2.73|P-value:9.78E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:4.66|P-value:6.58E-8 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.04|P-value:2.84E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.92|P-value:4.55E-6 ID:SGTC_1151|Compound:3909-8316|FD-Score:-3.20|P-value:1.53E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:-4.18|P-value:1.20E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:-3.96|P-value:4.03E-6 ID:SGTC_1157|Compound:4092-0394|FD-Score:3.44|P-value:4.79E-5 ID:SGTC_1164|Compound:k060-0068|FD-Score:2.76|P-value:8.72E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:2.95|P-value:4.06E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:-2.78|P-value:8.71E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.29|P-value:1.02E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:4.18|P-value:1.09E-6 ID:SGTC_1337|Compound:1486-1011|FD-Score:2.90|P-value:4.98E-4 ID:SGTC_1377|Compound:2944-0135|FD-Score:-2.88|P-value:5.88E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:3.09|P-value:2.33E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-2.80|P-value:7.92E-4 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.09|P-value:2.34E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.82|P-value:7.65E-6 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:3.41|P-value:5.66E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:2.82|P-value:6.86E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-3.01|P-value:3.52E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.07|P-value:2.47E-4 ID:SGTC_1676|Compound:st018489|FD-Score:-3.95|P-value:4.07E-6 ID:SGTC_1932|Compound:st074806|FD-Score:-2.76|P-value:9.45E-4 ID:SGTC_1936|Compound:st074407|FD-Score:5.72|P-value:4.74E-11 ID:SGTC_2240|Compound:6690706|FD-Score:-2.77|P-value:8.94E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:2.77|P-value:8.35E-4 ID:SGTC_2311|Compound:7694451|FD-Score:2.83|P-value:6.70E-4 ID:SGTC_2348|Compound:9022543|FD-Score:44.20|P-value:0 ID:SGTC_2375|Compound:9072683|FD-Score:2.91|P-value:4.87E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:5.02|P-value:6.45E-9 ID:SGTC_2460|Compound:5552655|FD-Score:3.60|P-value:2.29E-5 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.59|P-value:2.33E-5 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:2.90|P-value:4.98E-4 ID:SGTC_2900|Compound:5328528|FD-Score:-2.90|P-value:5.40E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.78|P-value:9.39E-6 ID:SGTC_2969|Compound:9091408|FD-Score:-2.95|P-value:4.39E-4 ID:SGTC_2970|Compound:9090938|FD-Score:2.75|P-value:9.09E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-2.96|P-value:4.19E-4 ID:SGTC_2995|Compound:9067767|FD-Score:-2.84|P-value:6.73E-4 ID:SGTC_3157|Compound:9099274|FD-Score:3.07|P-value:2.55E-4 ID:SGTC_3159|Compound:9100096|FD-Score:-3.05|P-value:2.93E-4 ID:SGTC_3208|Compound:9114115|FD-Score:3.12|P-value:2.00E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.21|P-value:1.38E-4 ID:SGTC_3291|Compound:9121177|FD-Score:2.82|P-value:6.96E-4

Top fitness defect scores for YDL140C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2348 9022543 44.20 33.4 µM 0 mitochondrial processes 1 0 25.80
2 SGTC_69 0096-0274 6.64 206.7 µM 2.85E-14 mitochondrial processes 14 1.58E-11 0.01
3 SGTC_1936 st074407 5.72 74.3 µM 4.74E-11 DNA damage response 3 5.35E-9 0.25
4 SGTC_2418 pentamidine 5.02 31.7 µM 6.45E-9 mitochondrial processes 10 2.55E-7 0.31
5 SGTC_562 3772-2733 4.95 112.0 µM 1.04E-8 ERG2 8 3.71E-7 0.44
6 SGTC_1067 clotrimazole 4.66 1.4 µM 6.58E-8 azole & statin 15 1.59E-6 0.08
7 SGTC_429 1112-0049 4.37 25.5 µM 3.80E-7 DNA damage response 6 6.33E-6 0.09
8 SGTC_1301 naphtanilide LB 4.18 4.8 µM 1.09E-6 heme requiring 6 1.46E-5 0.06
9 SGTC_194 1486-1323 3.96 56.9 µM 3.66E-6 10 3.77E-5 0.39
10 SGTC_1133 3473-0247 3.92 50.0 µM 4.55E-6 RSC complex & mRNA processing 46 4.48E-5 0.02
11 SGTC_1514 1189-1591 3.82 158.0 µM 7.65E-6 6 6.76E-5 0.13
12 SGTC_65 0798-0905 3.82 35.0 µM 7.68E-6 calcium & mitochondrial duress 9 6.78E-5 0.03
13 SGTC_2928 9011484 3.78 9.8 µM 9.39E-6 heme biosynthesis & mitochondrial translocase 31 7.95E-5 0.02
14 SGTC_2460 5552655 3.60 174.2 µM 2.29E-5 amide catabolism 12 1.61E-4 0.19
15 SGTC_2519 cryptotanshinone 3.59 88.0 µM 2.33E-5 heme biosynthesis & mitochondrial translocase 23 1.63E-4 0.01
16 SGTC_1157 4092-0394 3.44 27.6 µM 4.79E-5 6 2.88E-4 0.16
17 SGTC_1537 2',6'-dihydroxyflavanone 3.41 78.0 µM 5.66E-5 14 3.29E-4 0.21
18 SGTC_3284 9108171 3.21 64.9 µM 1.38E-4 9 6.68E-4 0.03
19 SGTC_603 3794-0023 3.19 45.9 µM 1.49E-4 4 7.09E-4 0.06
20 SGTC_3208 9114115 3.12 49.5 µM 2.00E-4 29 8.95E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.330 4.21E-86 YGR005C TFG2 het TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30
0.252 1.26E-49 YKL160W ELF1 hom Transcription elongation factor that contains a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
0.245 3.14E-47 YMR277W FCP1 het Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
0.215 1.94E-36 YPL169C MEX67 het Poly(A)RNA binding protein involved in nuclear mRNA export, component of the nuclear pore; ortholog of human TAP
0.208 3.20E-34 YKL103C APE1 hom Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to the peptidase family M18; often used as a marker protein in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress
0.202 2.61E-32 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.199 2.86E-31 YKL084W HOT13 hom Zinc-binding mitochondrial intermembrane space (IMS) protein involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc
0.181 4.40E-26 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.176 8.30E-25 YBL013W FMT1 hom Methionyl-tRNA formyltransferase, catalyzes the formylation of initiator Met-tRNA in mitochondria; potential Cdc28p substrate
0.176 8.48E-25 YOR042W CUE5 hom Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.175 1.27E-24 YNL038W GPI15 het Protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein
0.169 7.78E-23 YLR055C SPT8 hom Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.156 9.66E-20 YKL198C PTK1 hom Putative serine/threonine protein kinase that regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein
0.154 3.38E-19 YNL251C NRD1 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with the C-terminal domain of the RNA polymerase II large subunit (Rpo21p), preferentially at phosphorylated Ser5, to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites
0.148 6.59E-18 YJL002C OST1 het Alpha subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins