YDR484W / VPS52

Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR484W / VPS52

Click on Significant Values for Screen Details ID:SGTC_31|Compound:0508-1974|FD-Score:4.33|P-value:4.72E-4 ID:SGTC_45|Compound:0kpi-0036|FD-Score:4.50|P-value:2.88E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-7.89|P-value:5.27E-10 ID:SGTC_63|Compound:0099-0242|FD-Score:-5.47|P-value:1.12E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:-5.18|P-value:3.00E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:4.77|P-value:1.35E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:4.17|P-value:7.33E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.07|P-value:5.24E-5 ID:SGTC_232|Compound:clozapine|FD-Score:5.42|P-value:1.68E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:7.79|P-value:1.17E-9 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.99|P-value:6.76E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:-6.40|P-value:3.72E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-10.60|P-value:1.83E-16 ID:SGTC_455|Compound:paf C-16|FD-Score:-6.49|P-value:2.53E-7 ID:SGTC_459|Compound:nigericin|FD-Score:4.15|P-value:7.56E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:4.15|P-value:7.59E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:4.11|P-value:8.53E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:4.89|P-value:9.13E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:-4.11|P-value:7.09E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.30|P-value:5.05E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:4.67|P-value:1.77E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:-5.33|P-value:1.85E-5 ID:SGTC_968|Compound:1309-0633|FD-Score:-5.38|P-value:1.53E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:-4.55|P-value:2.08E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:4.47|P-value:3.16E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:5.41|P-value:1.73E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:5.38|P-value:1.93E-5 ID:SGTC_1220|Compound:1838-0075|FD-Score:-4.74|P-value:1.21E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:-4.03|P-value:8.86E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:-4.36|P-value:3.60E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:5.51|P-value:1.24E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:5.41|P-value:1.73E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:5.72|P-value:5.94E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.14|P-value:4.20E-5 ID:SGTC_1660|Compound:st012960|FD-Score:4.79|P-value:1.27E-4 ID:SGTC_1666|Compound:st014829|FD-Score:4.91|P-value:8.66E-5 ID:SGTC_1732|Compound:st034308|FD-Score:4.49|P-value:3.03E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-4.28|P-value:4.52E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-5.18|P-value:2.99E-5 ID:SGTC_2385|Compound:9074692|FD-Score:4.62|P-value:2.05E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.35|P-value:4.47E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-4.51|P-value:2.32E-4 ID:SGTC_2431|Compound:5490079|FD-Score:5.89|P-value:3.25E-6 ID:SGTC_2444|Compound:5485484|FD-Score:4.52|P-value:2.72E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.55|P-value:2.53E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:6.25|P-value:8.62E-7 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:5.02|P-value:6.17E-5 ID:SGTC_2602|Compound:kawain|FD-Score:-4.32|P-value:4.02E-4 ID:SGTC_2699|Compound:st077704|FD-Score:5.71|P-value:6.22E-6 ID:SGTC_2863|Compound:9028737|FD-Score:-4.16|P-value:6.19E-4 ID:SGTC_2886|Compound:9058944|FD-Score:-4.48|P-value:2.55E-4 ID:SGTC_2891|Compound:9063876|FD-Score:-4.01|P-value:9.18E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-4.24|P-value:5.04E-4 ID:SGTC_3086|Compound:9117350|FD-Score:-4.82|P-value:9.32E-5 ID:SGTC_3098|Compound:9119039|FD-Score:4.22|P-value:6.42E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.42|P-value:3.08E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-4.42|P-value:3.01E-4 ID:SGTC_3226|Compound:9131256|FD-Score:-4.86|P-value:8.23E-5 ID:SGTC_3230|Compound:9131665|FD-Score:-4.21|P-value:5.43E-4 ID:SGTC_3262|Compound:9137218|FD-Score:-4.53|P-value:2.20E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-4.29|P-value:4.39E-4 ID:SGTC_31|Compound:0508-1974|FD-Score:4.33|P-value:4.72E-4 ID:SGTC_45|Compound:0kpi-0036|FD-Score:4.50|P-value:2.88E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-7.89|P-value:5.27E-10 ID:SGTC_63|Compound:0099-0242|FD-Score:-5.47|P-value:1.12E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:-5.18|P-value:3.00E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:4.77|P-value:1.35E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:4.17|P-value:7.33E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.07|P-value:5.24E-5 ID:SGTC_232|Compound:clozapine|FD-Score:5.42|P-value:1.68E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:7.79|P-value:1.17E-9 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.99|P-value:6.76E-5 ID:SGTC_359|Compound:0081-0058|FD-Score:-6.40|P-value:3.72E-7 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-10.60|P-value:1.83E-16 ID:SGTC_455|Compound:paf C-16|FD-Score:-6.49|P-value:2.53E-7 ID:SGTC_459|Compound:nigericin|FD-Score:4.15|P-value:7.56E-4 ID:SGTC_553|Compound:k007-0256|FD-Score:4.15|P-value:7.59E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:4.11|P-value:8.53E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:4.89|P-value:9.13E-5 ID:SGTC_871|Compound:0574-0004|FD-Score:-4.11|P-value:7.09E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.30|P-value:5.05E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:4.67|P-value:1.77E-4 ID:SGTC_939|Compound:1165-0479|FD-Score:-5.33|P-value:1.85E-5 ID:SGTC_968|Compound:1309-0633|FD-Score:-5.38|P-value:1.53E-5 ID:SGTC_1007|Compound:4101-0031|FD-Score:-4.55|P-value:2.08E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:4.47|P-value:3.16E-4 ID:SGTC_1025|Compound:k007-0731|FD-Score:5.41|P-value:1.73E-5 ID:SGTC_1026|Compound:k007-1105|FD-Score:5.38|P-value:1.93E-5 ID:SGTC_1220|Compound:1838-0075|FD-Score:-4.74|P-value:1.21E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:-4.03|P-value:8.86E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:-4.36|P-value:3.60E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:5.51|P-value:1.24E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:5.41|P-value:1.73E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:5.72|P-value:5.94E-6 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.14|P-value:4.20E-5 ID:SGTC_1660|Compound:st012960|FD-Score:4.79|P-value:1.27E-4 ID:SGTC_1666|Compound:st014829|FD-Score:4.91|P-value:8.66E-5 ID:SGTC_1732|Compound:st034308|FD-Score:4.49|P-value:3.03E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-4.28|P-value:4.52E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-5.18|P-value:2.99E-5 ID:SGTC_2385|Compound:9074692|FD-Score:4.62|P-value:2.05E-4 ID:SGTC_2401|Compound:5128683|FD-Score:4.35|P-value:4.47E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-4.51|P-value:2.32E-4 ID:SGTC_2431|Compound:5490079|FD-Score:5.89|P-value:3.25E-6 ID:SGTC_2444|Compound:5485484|FD-Score:4.52|P-value:2.72E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.55|P-value:2.53E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:6.25|P-value:8.62E-7 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:5.02|P-value:6.17E-5 ID:SGTC_2602|Compound:kawain|FD-Score:-4.32|P-value:4.02E-4 ID:SGTC_2699|Compound:st077704|FD-Score:5.71|P-value:6.22E-6 ID:SGTC_2863|Compound:9028737|FD-Score:-4.16|P-value:6.19E-4 ID:SGTC_2886|Compound:9058944|FD-Score:-4.48|P-value:2.55E-4 ID:SGTC_2891|Compound:9063876|FD-Score:-4.01|P-value:9.18E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-4.24|P-value:5.04E-4 ID:SGTC_3086|Compound:9117350|FD-Score:-4.82|P-value:9.32E-5 ID:SGTC_3098|Compound:9119039|FD-Score:4.22|P-value:6.42E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.42|P-value:3.08E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-4.42|P-value:3.01E-4 ID:SGTC_3226|Compound:9131256|FD-Score:-4.86|P-value:8.23E-5 ID:SGTC_3230|Compound:9131665|FD-Score:-4.21|P-value:5.43E-4 ID:SGTC_3262|Compound:9137218|FD-Score:-4.53|P-value:2.20E-4 ID:SGTC_3265|Compound:9137266|FD-Score:-4.29|P-value:4.39E-4

Top fitness defect scores for YDR484W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_234 nsc-17383 7.79 10.7 µM 1.17E-9 ERG2 6 3.33E-15
2 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 6.25 15.3 µM 8.62E-7 7 2.10E-10
3 SGTC_2431 5490079 5.89 200.0 µM 3.25E-6 7 1.92E-9
4 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 5.72 54.9 µM 5.94E-6 redox potentiating 33 5.25E-9
5 SGTC_2699 st077704 5.71 54.6 µM 6.22E-6 endomembrane recycling 22 5.67E-9
6 SGTC_1409 3909-8004 5.51 61.9 µM 1.24E-5 2 1.80E-8
7 SGTC_232 clozapine 5.42 119.0 µM 1.68E-5 NEO1 37 2.97E-8
8 SGTC_1581 himbacine 5.41 57.9 µM 1.73E-5 Golgi 22 3.11E-8
9 SGTC_1025 k007-0731 5.41 70.0 µM 1.73E-5 2 3.12E-8
10 SGTC_1026 k007-1105 5.38 30.6 µM 1.93E-5 ERAD & cell cycle 53 3.74E-8
11 SGTC_1597 7-benzyloxygramine 5.14 47.5 µM 4.20E-5 calcium & mitochondrial duress 64 1.36E-7
12 SGTC_221 trifluoperazine 5.07 10.3 µM 5.24E-5 NEO1-PIK1 157 1.97E-7
13 SGTC_2578 7,8-dihydroxyflavone 5.02 64.4 µM 6.17E-5 15 2.58E-7
14 SGTC_275 trichlorophene 4.99 5.7 µM 6.76E-5 mitochondrial stress 166 3.00E-7
15 SGTC_1666 st014829 4.91 29.0 µM 8.66E-5 calcium & mitochondrial duress 15 4.53E-7
16 SGTC_821 1114-0266 4.89 29.2 µM 9.13E-5 8 4.95E-7
17 SGTC_1660 st012960 4.79 28.1 µM 1.27E-4 iron homeostasis 18 8.53E-7
18 SGTC_119 0121-0043 4.77 24.8 µM 1.35E-4 mitochondrial stress 67 9.43E-7
19 SGTC_936 3005-4620 4.67 401.0 µM 1.77E-4 12 1.48E-6
20 SGTC_2385 9074692 4.62 200.0 µM 2.05E-4 28 1.89E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.232 4.82E-42 YGL095C VPS45 hom Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
0.229 5.14E-41 YOL018C TLG2 hom Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
0.193 1.58E-29 YKR019C IRS4 hom EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
0.175 1.69E-24 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.171 2.14E-23 YGL054C ERV14 hom COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
0.169 9.40E-23 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.167 2.56E-22 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.166 4.05E-22 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.165 8.91E-22 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.161 8.45E-21 YKR020W VPS51 hom Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p
0.153 4.16E-19 YLR268W SEC22 hom R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
0.152 9.91E-19 YLR025W SNF7 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.150 2.26E-18 YNL041C COG6 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.150 2.86E-18 YDR455C_d YDR455C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
0.149 3.93E-18 YDL041W_d YDL041W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C