YEL037C / RAD23

Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover

Zygosity: Homozygous strain
fixedexpanded
Profile for YEL037C / RAD23

Click on Significant Values for Screen Details ID:SGTC_92|Compound:0063-0024|FD-Score:-3.28|P-value:7.60E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:3.85|P-value:6.79E-5 ID:SGTC_209|Compound:4077-0173|FD-Score:-3.57|P-value:2.76E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:14.30|P-value:3.66E-45 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.22|P-value:2.13E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.69|P-value:1.72E-6 ID:SGTC_496|Compound:calpeptin|FD-Score:3.18|P-value:7.97E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:3.43|P-value:3.30E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:3.61|P-value:1.71E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.25|P-value:6.11E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:-3.26|P-value:7.99E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-4.53|P-value:5.60E-6 ID:SGTC_982|Compound:1491-0518|FD-Score:-3.26|P-value:8.21E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.63|P-value:1.55E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.64|P-value:1.54E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.28|P-value:5.58E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.12|P-value:9.66E-4 ID:SGTC_1115|Compound:galangin|FD-Score:3.75|P-value:1.01E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.40|P-value:3.62E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:-5.12|P-value:3.30E-7 ID:SGTC_1189|Compound:1431-2119|FD-Score:-3.25|P-value:8.26E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.22|P-value:6.82E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.65|P-value:1.48E-4 ID:SGTC_1411|Compound:3909-8223|FD-Score:3.37|P-value:4.10E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-6.16|P-value:9.91E-10 ID:SGTC_1871|Compound:st057448|FD-Score:4.04|P-value:3.14E-5 ID:SGTC_2015|Compound:5119600|FD-Score:-3.20|P-value:9.81E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-3.76|P-value:1.35E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.14|P-value:3.02E-5 ID:SGTC_2139|Compound:5578692|FD-Score:4.82|P-value:9.09E-7 ID:SGTC_2337|Compound:9009472|FD-Score:3.23|P-value:6.71E-4 ID:SGTC_2812|Compound:7970281|FD-Score:-3.45|P-value:4.27E-4 ID:SGTC_2836|Compound:9010338|FD-Score:-3.44|P-value:4.30E-4 ID:SGTC_2963|Compound:9082507|FD-Score:-3.43|P-value:4.53E-4 ID:SGTC_3065|Compound:9113888|FD-Score:3.57|P-value:1.97E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-3.53|P-value:3.17E-4 ID:SGTC_3090|Compound:9117696|FD-Score:3.33|P-value:4.66E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.14|P-value:9.11E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.14|P-value:9.04E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-3.37|P-value:5.59E-4 ID:SGTC_92|Compound:0063-0024|FD-Score:-3.28|P-value:7.60E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:3.85|P-value:6.79E-5 ID:SGTC_209|Compound:4077-0173|FD-Score:-3.57|P-value:2.76E-4 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:14.30|P-value:3.66E-45 ID:SGTC_350|Compound:k064-0020|FD-Score:-4.22|P-value:2.13E-5 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.69|P-value:1.72E-6 ID:SGTC_496|Compound:calpeptin|FD-Score:3.18|P-value:7.97E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:3.43|P-value:3.30E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:3.61|P-value:1.71E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.25|P-value:6.11E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:-3.26|P-value:7.99E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-4.53|P-value:5.60E-6 ID:SGTC_982|Compound:1491-0518|FD-Score:-3.26|P-value:8.21E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:3.63|P-value:1.55E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.64|P-value:1.54E-4 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:3.28|P-value:5.58E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.12|P-value:9.66E-4 ID:SGTC_1115|Compound:galangin|FD-Score:3.75|P-value:1.01E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.40|P-value:3.62E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:-5.12|P-value:3.30E-7 ID:SGTC_1189|Compound:1431-2119|FD-Score:-3.25|P-value:8.26E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.22|P-value:6.82E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.65|P-value:1.48E-4 ID:SGTC_1411|Compound:3909-8223|FD-Score:3.37|P-value:4.10E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:-6.16|P-value:9.91E-10 ID:SGTC_1871|Compound:st057448|FD-Score:4.04|P-value:3.14E-5 ID:SGTC_2015|Compound:5119600|FD-Score:-3.20|P-value:9.81E-4 ID:SGTC_2018|Compound:5133068|FD-Score:-3.76|P-value:1.35E-4 ID:SGTC_2021|Compound:4019055|FD-Score:-4.14|P-value:3.02E-5 ID:SGTC_2139|Compound:5578692|FD-Score:4.82|P-value:9.09E-7 ID:SGTC_2337|Compound:9009472|FD-Score:3.23|P-value:6.71E-4 ID:SGTC_2812|Compound:7970281|FD-Score:-3.45|P-value:4.27E-4 ID:SGTC_2836|Compound:9010338|FD-Score:-3.44|P-value:4.30E-4 ID:SGTC_2963|Compound:9082507|FD-Score:-3.43|P-value:4.53E-4 ID:SGTC_3065|Compound:9113888|FD-Score:3.57|P-value:1.97E-4 ID:SGTC_3072|Compound:9116419|FD-Score:-3.53|P-value:3.17E-4 ID:SGTC_3090|Compound:9117696|FD-Score:3.33|P-value:4.66E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.14|P-value:9.11E-4 ID:SGTC_3238|Compound:9133470|FD-Score:3.14|P-value:9.04E-4 ID:SGTC_3321|Compound:9137793|FD-Score:-3.37|P-value:5.59E-4

Top fitness defect scores for YEL037C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 14.30 44.4 nM 3.66E-45 DNA damage response 7 1.24E-46
2 SGTC_2139 5578692 4.82 56.5 µM 9.09E-7 superoxide 10 7.05E-7
3 SGTC_450 aminopyrene 4.69 156.0 nM 1.72E-6 19 1.36E-6
4 SGTC_1871 st057448 4.04 67.3 µM 3.14E-5 25 2.70E-5
5 SGTC_193 1273-0060 3.85 1.6 µM 6.79E-5 ergosterol biosynthesis 10 5.97E-5
6 SGTC_1115 galangin 3.75 695.7 nM 1.01E-4 RPP1 & pyrimidine depletion 20 9.01E-5
7 SGTC_1306 1185-0270 3.65 9.0 µM 1.48E-4 Golgi 57 1.32E-4
8 SGTC_1040 k898-0338 3.64 155.0 µM 1.54E-4 28 1.38E-4
9 SGTC_1039 k821-0043 3.63 94.0 µM 1.55E-4 Golgi 39 1.40E-4
10 SGTC_654 1124-0271 3.61 80.9 µM 1.71E-4 36 1.54E-4
11 SGTC_3065 9113888 3.57 49.5 µM 1.97E-4 34 1.78E-4
12 SGTC_504 lovastatin 3.43 124.0 µM 3.30E-4 24 3.03E-4
13 SGTC_1181 1222-0449 3.40 220.0 µM 3.62E-4 42 3.33E-4
14 SGTC_1411 3909-8223 3.37 150.0 µM 4.10E-4 25 3.78E-4
15 SGTC_3090 9117696 3.33 49.5 µM 4.66E-4 29 4.31E-4
16 SGTC_1099 5-nonyloxytryptamine 3.28 2.9 µM 5.58E-4 44 5.19E-4
17 SGTC_951 1171-0579 3.25 82.7 µM 6.11E-4 cell wall 123 5.69E-4
18 SGTC_2337 9009472 3.23 200.0 µM 6.71E-4 63 6.27E-4
19 SGTC_1213 1315-0166 3.22 115.0 µM 6.82E-4 53 6.37E-4
20 SGTC_496 calpeptin 3.18 138.0 µM 7.97E-4 43 7.47E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.440 3.58E-159 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.434 1.46E-154 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.429 2.37E-150 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.350 2.38E-97 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.339 3.85E-91 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.229 4.92E-41 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.196 1.70E-30 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.153 6.35E-19 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.149 3.57E-18 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.147 1.29E-17 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.128 8.24E-14 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.121 1.83E-12 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.118 8.35E-12 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.117 1.16E-11 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.103 2.16E-9 YFL047W RGD2 hom GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress