YER064C / VHR2

Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YER064C / VHR2

Click on Significant Values for Screen Details

Top fitness defect scores for YER064C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_685 1278-0033 4.12 54.9 µM 2.77E-6 60S ribosome export 17 1.91E-5
2 SGTC_780 0109-0199 3.96 192.0 µM 6.11E-6 13 3.68E-5
3 SGTC_2351 9026858 3.85 72.3 µM 1.09E-5 RNA processing & uracil transport 27 5.96E-5
4 SGTC_2312 7706453 3.78 155.7 µM 1.49E-5 30 7.71E-5
5 SGTC_3209 9113616 3.65 49.5 µM 2.79E-5 37 1.30E-4
6 SGTC_432 NS 1619 3.51 61.3 µM 5.30E-5 42 2.21E-4
7 SGTC_189 3970-0742 3.13 2.1 µM 2.72E-4 20 8.61E-4
8 SGTC_851 0394-0702 2.98 453.0 µM 5.08E-4 mitochondrial processes 43 0.00145
9 SGTC_1831 st055328 2.98 12.0 µM 5.14E-4 heme biosynthesis & mitochondrial translocase 67 0.00146
10 SGTC_361 000s-0510 2.95 1.4 µM 5.76E-4 88 0.00161
11 SGTC_2190 6049624 2.94 200.0 µM 5.82E-4 28 0.00162
12 SGTC_73 0327-0325 2.93 16.2 µM 6.20E-4 DNA damage response 127 0.00171
13 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 2.91 3.7 µM 6.63E-4 225 0.00181
14 SGTC_2657 nystatin 2.91 74.4 µM 6.65E-4 54 0.00182
15 SGTC_705 1124-0212 2.87 310.0 µM 7.62E-4 63 0.00203
16 SGTC_1914 st062367 2.85 72.6 µM 8.19E-4 71 0.00216
17 SGTC_2389 9091542 2.83 195.5 µM 9.07E-4 iron homeostasis 101 0.00235
18 SGTC_1838 st055369 2.79 84.5 µM 0.00105 64 0.00266
19 SGTC_2568 diosmetin 2.76 100.0 µM 0.00115 91 0.00286
20 SGTC_245 methotrexate 2.72 289.6 µM 0.00134 112 0.00325

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.323 1.69E-82 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.291 2.66E-66 YBL086C_p YBL086C_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.286 3.52E-64 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.278 2.21E-60 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.261 2.89E-53 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.247 4.94E-48 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
0.246 1.21E-47 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.244 8.37E-47 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.243 2.33E-46 YMR139W RIM11 hom Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress
0.242 5.77E-46 YJL068C YJL068C hom Non-essential intracellular esterase that can function as an S-formylglutathione hydrolase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
0.240 2.87E-45 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.232 3.57E-42 YCL035C GRX1 hom Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.229 3.46E-41 YFR027W ECO1 het Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
0.228 1.27E-40 YER071C_p TDA2_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; null mutant is sensitive to expression of the top1-T722A allele
0.227 2.26E-40 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing