YFL029C / CAK1

Cyclin-dependent kinase-activating kinase required for passage through the cell cycle, phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases

Zygosity: Heterozygous strain
fixedexpanded
Profile for YFL029C / CAK1

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:-3.95|P-value:2.03E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.69|P-value:5.21E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:3.57|P-value:7.59E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.61|P-value:6.71E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.55|P-value:8.22E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:4.94|P-value:5.80E-6 ID:SGTC_433|Compound:pimozide|FD-Score:5.58|P-value:3.54E-7 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.68|P-value:5.39E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:3.74|P-value:4.52E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:3.64|P-value:6.08E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:4.86|P-value:7.80E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.63|P-value:6.32E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:3.57|P-value:7.62E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:-3.78|P-value:3.58E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:7.50|P-value:1.28E-11 ID:SGTC_857|Compound:0438-0306|FD-Score:7.77|P-value:2.34E-12 ID:SGTC_874|Compound:0599-0792|FD-Score:-3.76|P-value:3.78E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:5.06|P-value:3.41E-6 ID:SGTC_1098|Compound:clofazimine|FD-Score:-3.72|P-value:4.34E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.95|P-value:2.05E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:6.46|P-value:4.69E-9 ID:SGTC_1273|Compound:0849-0096|FD-Score:4.13|P-value:1.22E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.41|P-value:4.42E-5 ID:SGTC_1720|Compound:st037298|FD-Score:4.08|P-value:1.48E-4 ID:SGTC_2025|Compound:5147754|FD-Score:3.52|P-value:8.80E-4 ID:SGTC_2109|Compound:5483026|FD-Score:5.64|P-value:2.64E-7 ID:SGTC_2121|Compound:5257820|FD-Score:4.40|P-value:4.72E-5 ID:SGTC_2379|Compound:9035213|FD-Score:3.48|P-value:9.97E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.81|P-value:3.62E-4 ID:SGTC_2412|Compound:st077135|FD-Score:5.06|P-value:3.45E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.19|P-value:9.05E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-4.06|P-value:1.40E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.70|P-value:5.11E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.86|P-value:2.81E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:4.56|P-value:2.60E-5 ID:SGTC_2729|Compound:fluphenazine|FD-Score:6.55|P-value:2.82E-9 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.91|P-value:2.61E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.66|P-value:1.74E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.72|P-value:4.87E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-3.65|P-value:5.39E-4 ID:SGTC_2849|Compound:9019321|FD-Score:5.40|P-value:7.83E-7 ID:SGTC_2881|Compound:9058953|FD-Score:5.42|P-value:7.43E-7 ID:SGTC_2928|Compound:9011484|FD-Score:-3.88|P-value:2.56E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.59|P-value:7.18E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.61|P-value:6.84E-4 ID:SGTC_2970|Compound:9090938|FD-Score:4.34|P-value:5.75E-5 ID:SGTC_2991|Compound:9074669|FD-Score:-4.91|P-value:5.76E-6 ID:SGTC_2998|Compound:9066762|FD-Score:6.22|P-value:1.57E-8 ID:SGTC_3067|Compound:9117431|FD-Score:4.42|P-value:4.33E-5 ID:SGTC_3085|Compound:9117376|FD-Score:5.44|P-value:6.81E-7 ID:SGTC_3087|Compound:9117406|FD-Score:-3.52|P-value:8.06E-4 ID:SGTC_3112|Compound:9123419|FD-Score:-4.68|P-value:1.42E-5 ID:SGTC_3115|Compound:9123671|FD-Score:-3.66|P-value:5.33E-4 ID:SGTC_3147|Compound:9097027|FD-Score:4.73|P-value:1.33E-5 ID:SGTC_3152|Compound:9099253|FD-Score:5.59|P-value:3.32E-7 ID:SGTC_3154|Compound:9100113|FD-Score:-3.72|P-value:4.34E-4 ID:SGTC_3185|Compound:9108267|FD-Score:4.62|P-value:2.04E-5 ID:SGTC_3219|Compound:9131112|FD-Score:3.65|P-value:5.94E-4 ID:SGTC_3259|Compound:9138485|FD-Score:9.20|P-value:1.37E-16 ID:SGTC_3262|Compound:9137218|FD-Score:3.57|P-value:7.74E-4 ID:SGTC_3278|Compound:9139605|FD-Score:3.67|P-value:5.58E-4 ID:SGTC_3317|Compound:9138495|FD-Score:4.09|P-value:1.43E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:-3.95|P-value:2.03E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.69|P-value:5.21E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:3.57|P-value:7.59E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.61|P-value:6.71E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.55|P-value:8.22E-4 ID:SGTC_428|Compound:3448-4153|FD-Score:4.94|P-value:5.80E-6 ID:SGTC_433|Compound:pimozide|FD-Score:5.58|P-value:3.54E-7 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.68|P-value:5.39E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:3.74|P-value:4.52E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:3.64|P-value:6.08E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:4.86|P-value:7.80E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.63|P-value:6.32E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:3.57|P-value:7.62E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:-3.78|P-value:3.58E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:7.50|P-value:1.28E-11 ID:SGTC_857|Compound:0438-0306|FD-Score:7.77|P-value:2.34E-12 ID:SGTC_874|Compound:0599-0792|FD-Score:-3.76|P-value:3.78E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:5.06|P-value:3.41E-6 ID:SGTC_1098|Compound:clofazimine|FD-Score:-3.72|P-value:4.34E-4 ID:SGTC_1128|Compound:3448-9350|FD-Score:-3.95|P-value:2.05E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:6.46|P-value:4.69E-9 ID:SGTC_1273|Compound:0849-0096|FD-Score:4.13|P-value:1.22E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.41|P-value:4.42E-5 ID:SGTC_1720|Compound:st037298|FD-Score:4.08|P-value:1.48E-4 ID:SGTC_2025|Compound:5147754|FD-Score:3.52|P-value:8.80E-4 ID:SGTC_2109|Compound:5483026|FD-Score:5.64|P-value:2.64E-7 ID:SGTC_2121|Compound:5257820|FD-Score:4.40|P-value:4.72E-5 ID:SGTC_2379|Compound:9035213|FD-Score:3.48|P-value:9.97E-4 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.81|P-value:3.62E-4 ID:SGTC_2412|Compound:st077135|FD-Score:5.06|P-value:3.45E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.19|P-value:9.05E-5 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:-4.06|P-value:1.40E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.70|P-value:5.11E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.86|P-value:2.81E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:4.56|P-value:2.60E-5 ID:SGTC_2729|Compound:fluphenazine|FD-Score:6.55|P-value:2.82E-9 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.91|P-value:2.61E-4 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:4.66|P-value:1.74E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.72|P-value:4.87E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-3.65|P-value:5.39E-4 ID:SGTC_2849|Compound:9019321|FD-Score:5.40|P-value:7.83E-7 ID:SGTC_2881|Compound:9058953|FD-Score:5.42|P-value:7.43E-7 ID:SGTC_2928|Compound:9011484|FD-Score:-3.88|P-value:2.56E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.59|P-value:7.18E-4 ID:SGTC_2966|Compound:9084041|FD-Score:3.61|P-value:6.84E-4 ID:SGTC_2970|Compound:9090938|FD-Score:4.34|P-value:5.75E-5 ID:SGTC_2991|Compound:9074669|FD-Score:-4.91|P-value:5.76E-6 ID:SGTC_2998|Compound:9066762|FD-Score:6.22|P-value:1.57E-8 ID:SGTC_3067|Compound:9117431|FD-Score:4.42|P-value:4.33E-5 ID:SGTC_3085|Compound:9117376|FD-Score:5.44|P-value:6.81E-7 ID:SGTC_3087|Compound:9117406|FD-Score:-3.52|P-value:8.06E-4 ID:SGTC_3112|Compound:9123419|FD-Score:-4.68|P-value:1.42E-5 ID:SGTC_3115|Compound:9123671|FD-Score:-3.66|P-value:5.33E-4 ID:SGTC_3147|Compound:9097027|FD-Score:4.73|P-value:1.33E-5 ID:SGTC_3152|Compound:9099253|FD-Score:5.59|P-value:3.32E-7 ID:SGTC_3154|Compound:9100113|FD-Score:-3.72|P-value:4.34E-4 ID:SGTC_3185|Compound:9108267|FD-Score:4.62|P-value:2.04E-5 ID:SGTC_3219|Compound:9131112|FD-Score:3.65|P-value:5.94E-4 ID:SGTC_3259|Compound:9138485|FD-Score:9.20|P-value:1.37E-16 ID:SGTC_3262|Compound:9137218|FD-Score:3.57|P-value:7.74E-4 ID:SGTC_3278|Compound:9139605|FD-Score:3.67|P-value:5.58E-4 ID:SGTC_3317|Compound:9138495|FD-Score:4.09|P-value:1.43E-4

Top fitness defect scores for YFL029C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3259 9138485 9.20 49.5 µM 1.37E-16 1 1.82E-20 3.59
2 SGTC_857 0438-0306 7.77 1.4 µM 2.34E-12 copper-dependent oxidative stress 1 3.80E-15 3.43
3 SGTC_848 0368-0089 7.50 85.6 µM 1.28E-11 iron homeostasis 1 3.22E-14 1.00
4 SGTC_2729 fluphenazine 6.55 10.7 µM 2.82E-9 2 2.81E-11 1.25
5 SGTC_1133 3473-0247 6.46 50.0 µM 4.69E-9 RSC complex & mRNA processing 18 5.33E-11 0.12
6 SGTC_2998 9066762 6.22 71.4 µM 1.57E-8 RPP1 & pyrimidine depletion 3 2.44E-10 1.06
7 SGTC_2109 5483026 5.64 200.0 µM 2.64E-7 RSC complex & mRNA processing 21 8.35E-9 0.08
8 SGTC_3152 9099253 5.59 49.5 µM 3.32E-7 NEO1-PIK1 15 1.11E-8 0.57
9 SGTC_433 pimozide 5.58 108.0 µM 3.54E-7 1 1.21E-8 1.68
10 SGTC_3085 9117376 5.44 49.5 µM 6.81E-7 3 2.74E-8 0.59
11 SGTC_2881 9058953 5.42 52.0 µM 7.43E-7 1 3.05E-8 1.19
12 SGTC_2849 9019321 5.40 13.0 µM 7.83E-7 1 3.26E-8 0.64
13 SGTC_1006 4099-6547 5.06 255.0 µM 3.41E-6 2 2.06E-7 0.47
14 SGTC_2412 st077135 5.06 33.4 µM 3.45E-6 3 2.09E-7 0.14
15 SGTC_428 3448-4153 4.94 16.5 µM 5.80E-6 TSC3-RPN4 4 4.00E-7 0.27
16 SGTC_632 0781-2353 4.86 4.7 µM 7.80E-6 Golgi 9 5.80E-7 0.07
17 SGTC_3147 9097027 4.73 49.5 µM 1.33E-5 3 1.13E-6 0.35
18 SGTC_2751 anetholtrithion 4.66 17.2 µM 1.74E-5 2 1.58E-6 0.08
19 SGTC_3185 9108267 4.62 49.5 µM 2.04E-5 60S ribosome export 3 1.93E-6 0.47
20 SGTC_2620 irigenin 4.56 100.0 µM 2.60E-5 9 2.61E-6 0.15

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.081 2.34E-6 YKR031C SPO14 hom Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
0.081 2.80E-6 YNL265C IST1 hom Protein with a positive role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p
0.081 2.86E-6 YDR468C TLG1 het Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
0.080 3.14E-6 YCL054W SPB1 het AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
0.077 8.82E-6 YOL106W_d YOL106W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.076 1.04E-5 YPR034W ARP7 het Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
0.074 1.65E-5 YGR180C RNR4 hom Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.071 3.56E-5 YCR057C PWP2 het Conserved 90S pre-ribosomal component essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
0.071 3.92E-5 YPR041W TIF5 het Translation initiation factor eIF5; functions both as a GTPase-activating protein to mediate hydrolysis of ribosome-bound GTP and as a GDP dissociation inhibitor to prevent recycling of eIF2
0.068 8.17E-5 YOR260W GCD1 het Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.068 9.07E-5 YIL137C TMA108 hom Protein that associates with ribosomes and is involved in ribosome biogenesis; putative metalloprotease
0.067 1.14E-4 YLR424W SPP382 het Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
0.065 1.60E-4 YOL026C MIM1 het Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
0.065 1.62E-4 YDL210W UGA4 hom Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
0.065 1.78E-4 YER024W YAT2 hom Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane