YGL063W / PUS2

Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification

Zygosity: Homozygous strain
fixedexpanded
Profile for YGL063W / PUS2

Click on Significant Values for Screen Details ID:SGTC_21|Compound:0849-0113|FD-Score:3.39|P-value:3.62E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:3.29|P-value:5.23E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:7.05|P-value:1.35E-12 ID:SGTC_175|Compound:3793-3120|FD-Score:3.29|P-value:5.33E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:3.69|P-value:1.19E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.71|P-value:1.12E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.44|P-value:3.09E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-4.19|P-value:1.90E-5 ID:SGTC_366|Compound:0181-0206|FD-Score:3.42|P-value:3.27E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:3.12|P-value:9.48E-4 ID:SGTC_456|Compound:gingerol|FD-Score:-3.99|P-value:4.46E-5 ID:SGTC_516|Compound:3992-2719|FD-Score:4.60|P-value:2.44E-6 ID:SGTC_574|Compound:1315-0397|FD-Score:3.68|P-value:1.27E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:4.66|P-value:1.82E-6 ID:SGTC_643|Compound:k048-0106|FD-Score:4.06|P-value:2.66E-5 ID:SGTC_657|Compound:3534-0529|FD-Score:4.52|P-value:3.49E-6 ID:SGTC_939|Compound:1165-0479|FD-Score:5.34|P-value:5.73E-8 ID:SGTC_942|Compound:3253-1115|FD-Score:3.51|P-value:2.41E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.27|P-value:1.08E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:-4.34|P-value:1.01E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.30|P-value:5.06E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:6.46|P-value:7.44E-11 ID:SGTC_1148|Compound:3909-7663|FD-Score:-3.16|P-value:9.75E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.66|P-value:1.34E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:6.13|P-value:5.73E-10 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.20|P-value:7.25E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.28|P-value:5.43E-4 ID:SGTC_1653|Compound:st012112|FD-Score:-4.00|P-value:4.29E-5 ID:SGTC_1722|Compound:st034304|FD-Score:3.35|P-value:4.21E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.12|P-value:9.32E-4 ID:SGTC_1779|Compound:st039427|FD-Score:-3.95|P-value:5.20E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.20|P-value:1.45E-5 ID:SGTC_1845|Compound:st056753|FD-Score:5.18|P-value:1.32E-7 ID:SGTC_1908|Compound:st060353|FD-Score:3.91|P-value:4.91E-5 ID:SGTC_1936|Compound:st074407|FD-Score:3.43|P-value:3.17E-4 ID:SGTC_1977|Compound:st070720|FD-Score:3.76|P-value:9.14E-5 ID:SGTC_2053|Compound:5265679|FD-Score:3.21|P-value:6.88E-4 ID:SGTC_2055|Compound:5235184|FD-Score:4.45|P-value:4.76E-6 ID:SGTC_2060|Compound:5238662|FD-Score:6.09|P-value:7.38E-10 ID:SGTC_2182|Compound:6325192|FD-Score:-3.27|P-value:6.84E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.23|P-value:6.52E-4 ID:SGTC_2394|Compound:5221648|FD-Score:5.12|P-value:1.79E-7 ID:SGTC_2395|Compound:5646831|FD-Score:-5.24|P-value:1.28E-7 ID:SGTC_2396|Compound:5342950|FD-Score:-3.88|P-value:7.03E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.15|P-value:8.38E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.53|P-value:2.22E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.80|P-value:7.71E-5 ID:SGTC_2463|Compound:5473429|FD-Score:3.50|P-value:2.45E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:8.13|P-value:3.66E-16 ID:SGTC_2593|Compound:nobiletin|FD-Score:-4.74|P-value:1.59E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-3.75|P-value:1.16E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-4.53|P-value:4.35E-6 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-4.20|P-value:1.85E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-4.45|P-value:6.11E-6 ID:SGTC_2623|Compound:humulene|FD-Score:3.30|P-value:5.11E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.67|P-value:1.28E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.68|P-value:1.52E-4 ID:SGTC_2826|Compound:7992541|FD-Score:4.03|P-value:3.02E-5 ID:SGTC_2954|Compound:9074818|FD-Score:3.15|P-value:8.38E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.60|P-value:2.08E-4 ID:SGTC_3181|Compound:9108277|FD-Score:3.81|P-value:7.45E-5 ID:SGTC_3199|Compound:9111318|FD-Score:3.45|P-value:2.96E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.36|P-value:4.10E-4 ID:SGTC_21|Compound:0849-0113|FD-Score:3.39|P-value:3.62E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:3.29|P-value:5.23E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:7.05|P-value:1.35E-12 ID:SGTC_175|Compound:3793-3120|FD-Score:3.29|P-value:5.33E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:3.69|P-value:1.19E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.71|P-value:1.12E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.44|P-value:3.09E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-4.19|P-value:1.90E-5 ID:SGTC_366|Compound:0181-0206|FD-Score:3.42|P-value:3.27E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:3.12|P-value:9.48E-4 ID:SGTC_456|Compound:gingerol|FD-Score:-3.99|P-value:4.46E-5 ID:SGTC_516|Compound:3992-2719|FD-Score:4.60|P-value:2.44E-6 ID:SGTC_574|Compound:1315-0397|FD-Score:3.68|P-value:1.27E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:4.66|P-value:1.82E-6 ID:SGTC_643|Compound:k048-0106|FD-Score:4.06|P-value:2.66E-5 ID:SGTC_657|Compound:3534-0529|FD-Score:4.52|P-value:3.49E-6 ID:SGTC_939|Compound:1165-0479|FD-Score:5.34|P-value:5.73E-8 ID:SGTC_942|Compound:3253-1115|FD-Score:3.51|P-value:2.41E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.27|P-value:1.08E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:-4.34|P-value:1.01E-5 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.30|P-value:5.06E-4 ID:SGTC_1146|Compound:3579-1023|FD-Score:6.46|P-value:7.44E-11 ID:SGTC_1148|Compound:3909-7663|FD-Score:-3.16|P-value:9.75E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.66|P-value:1.34E-4 ID:SGTC_1315|Compound:1237-0019|FD-Score:6.13|P-value:5.73E-10 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.20|P-value:7.25E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.28|P-value:5.43E-4 ID:SGTC_1653|Compound:st012112|FD-Score:-4.00|P-value:4.29E-5 ID:SGTC_1722|Compound:st034304|FD-Score:3.35|P-value:4.21E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.12|P-value:9.32E-4 ID:SGTC_1779|Compound:st039427|FD-Score:-3.95|P-value:5.20E-5 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.20|P-value:1.45E-5 ID:SGTC_1845|Compound:st056753|FD-Score:5.18|P-value:1.32E-7 ID:SGTC_1908|Compound:st060353|FD-Score:3.91|P-value:4.91E-5 ID:SGTC_1936|Compound:st074407|FD-Score:3.43|P-value:3.17E-4 ID:SGTC_1977|Compound:st070720|FD-Score:3.76|P-value:9.14E-5 ID:SGTC_2053|Compound:5265679|FD-Score:3.21|P-value:6.88E-4 ID:SGTC_2055|Compound:5235184|FD-Score:4.45|P-value:4.76E-6 ID:SGTC_2060|Compound:5238662|FD-Score:6.09|P-value:7.38E-10 ID:SGTC_2182|Compound:6325192|FD-Score:-3.27|P-value:6.84E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.23|P-value:6.52E-4 ID:SGTC_2394|Compound:5221648|FD-Score:5.12|P-value:1.79E-7 ID:SGTC_2395|Compound:5646831|FD-Score:-5.24|P-value:1.28E-7 ID:SGTC_2396|Compound:5342950|FD-Score:-3.88|P-value:7.03E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.15|P-value:8.38E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.53|P-value:2.22E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.80|P-value:7.71E-5 ID:SGTC_2463|Compound:5473429|FD-Score:3.50|P-value:2.45E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:8.13|P-value:3.66E-16 ID:SGTC_2593|Compound:nobiletin|FD-Score:-4.74|P-value:1.59E-6 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-3.75|P-value:1.16E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:-4.53|P-value:4.35E-6 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-4.20|P-value:1.85E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-4.45|P-value:6.11E-6 ID:SGTC_2623|Compound:humulene|FD-Score:3.30|P-value:5.11E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.67|P-value:1.28E-4 ID:SGTC_2801|Compound:7907800|FD-Score:-3.68|P-value:1.52E-4 ID:SGTC_2826|Compound:7992541|FD-Score:4.03|P-value:3.02E-5 ID:SGTC_2954|Compound:9074818|FD-Score:3.15|P-value:8.38E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.60|P-value:2.08E-4 ID:SGTC_3181|Compound:9108277|FD-Score:3.81|P-value:7.45E-5 ID:SGTC_3199|Compound:9111318|FD-Score:3.45|P-value:2.96E-4 ID:SGTC_3203|Compound:9113543|FD-Score:3.36|P-value:4.10E-4

Top fitness defect scores for YGL063W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2592 piperlongumine 8.13 83.3 µM 3.66E-16 TSC3-RPN4 3 2.08E-16
2 SGTC_69 0096-0274 7.05 206.7 µM 1.35E-12 mitochondrial processes 6 9.04E-13
3 SGTC_1146 3579-1023 6.46 7.0 µM 7.44E-11 3 5.38E-11
4 SGTC_1315 1237-0019 6.13 3.7 µM 5.73E-10 1 4.31E-10
5 SGTC_2060 5238662 6.09 140.0 µM 7.38E-10 4 5.58E-10
6 SGTC_939 1165-0479 5.34 45.3 µM 5.73E-8 6 4.70E-8
7 SGTC_1845 st056753 5.18 3.0 µM 1.32E-7 TSC3-RPN4 17 1.10E-7
8 SGTC_2394 5221648 5.12 200.0 µM 1.79E-7 9 1.49E-7
9 SGTC_588 3970-1144 4.66 52.9 µM 1.82E-6 15 1.59E-6
10 SGTC_516 3992-2719 4.60 87.3 µM 2.44E-6 10 2.14E-6
11 SGTC_657 3534-0529 4.52 86.7 µM 3.49E-6 7 3.08E-6
12 SGTC_2055 5235184 4.45 200.0 µM 4.76E-6 ERG2 19 4.22E-6
13 SGTC_951 1171-0579 4.27 82.7 µM 1.08E-5 cell wall 63 9.70E-6
14 SGTC_1785 bathophenanthroline 4.20 24.0 µM 1.45E-5 heme requiring 39 1.31E-5
15 SGTC_643 k048-0106 4.06 28.4 µM 2.66E-5 RSC & ERG11 10 2.43E-5
16 SGTC_2826 7992541 4.03 19.5 µM 3.02E-5 18 2.76E-5
17 SGTC_1908 st060353 3.91 56.5 µM 4.91E-5 RPP1 & pyrimidine depletion 37 4.52E-5
18 SGTC_3181 9108277 3.81 49.5 µM 7.45E-5 iron homeostasis 21 6.91E-5
19 SGTC_2418 pentamidine 3.80 31.7 µM 7.71E-5 mitochondrial processes 50 7.16E-5
20 SGTC_1977 st070720 3.76 65.3 µM 9.14E-5 13 8.51E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.106 7.25E-10 YMR254C_d YMR254C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.095 2.98E-8 YLR297W YLR297W hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
0.091 1.28E-7 YGL062W PYC1 hom Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
0.089 2.34E-7 YDL026W_d YDL026W_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.089 2.68E-7 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.089 2.79E-7 YDL178W DLD2 hom D-lactate dehydrogenase, located in the mitochondrial matrix
0.084 1.24E-6 YNL059C ARP5 hom Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.083 1.41E-6 YPL211W NIP7 het Nucleolar protein required for 60S ribosome subunit biogenesis, constituent of 66S pre-ribosomal particles; physically interacts with Nop8p and the exosome subunit Rrp43p
0.083 1.63E-6 YBR265W TSC10 het 3-ketosphinganine reductase, catalyzes the second step in phytosphingosine synthesis, essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine, member of short chain dehydrogenase/reductase protein family
0.081 2.32E-6 YKL006C-A SFT1 het Intra-Golgi v-SNARE, required for transport of proteins between an early and a later Golgi compartment
0.081 2.62E-6 YER123W YCK3 hom Palmitoylated, vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; regulates vesicle fusion in AP-3 pathway
0.077 8.41E-6 YMR062C ARG7 hom Mitochondrial ornithine acetyltransferase, catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
0.076 1.13E-5 YKL043W PHD1 hom Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication
0.076 1.15E-5 YIL164C NIT1 hom Nitrilase, member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene
0.076 1.25E-5 YOR092W ECM3 hom Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication