YHR042W / NCP1

NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR042W / NCP1

Click on Significant Values for Screen Details ID:SGTC_11|Compound:0438-0307|FD-Score:9.68|P-value:1.17E-26 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.51|P-value:7.61E-5 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.02|P-value:5.70E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:4.64|P-value:1.57E-7 ID:SGTC_227|Compound:fluconazole|FD-Score:6.17|P-value:5.42E-12 ID:SGTC_256|Compound:clotrimazole|FD-Score:5.28|P-value:2.91E-9 ID:SGTC_268|Compound:camptothecin|FD-Score:-3.11|P-value:4.09E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.10|P-value:2.99E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.09|P-value:3.06E-4 ID:SGTC_548|Compound:1436-0017|FD-Score:-3.39|P-value:1.26E-4 ID:SGTC_555|Compound:4296-0685|FD-Score:-3.29|P-value:1.97E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:-3.32|P-value:1.69E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:6.75|P-value:5.46E-14 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.05|P-value:5.12E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:4.72|P-value:9.65E-8 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.67|P-value:3.73E-5 ID:SGTC_873|Compound:0591-4978|FD-Score:2.83|P-value:8.52E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.09|P-value:4.36E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:3.13|P-value:2.58E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:7.75|P-value:8.02E-18 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.13|P-value:3.66E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.14|P-value:2.46E-6 ID:SGTC_1507|Compound:4075-3230|FD-Score:3.05|P-value:3.62E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.68|P-value:2.37E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:12.10|P-value:2.88E-40 ID:SGTC_2010|Compound:4012422|FD-Score:4.56|P-value:2.37E-7 ID:SGTC_2030|Compound:5175110|FD-Score:3.38|P-value:9.16E-5 ID:SGTC_2273|Compound:7815444|FD-Score:3.24|P-value:1.69E-4 ID:SGTC_2285|Compound:7945142|FD-Score:7.17|P-value:1.62E-15 ID:SGTC_2307|Compound:7538926|FD-Score:5.35|P-value:1.82E-9 ID:SGTC_2314|Compound:7727897|FD-Score:-3.35|P-value:1.54E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.75|P-value:1.70E-5 ID:SGTC_2398|Compound:5228213|FD-Score:2.98|P-value:4.66E-4 ID:SGTC_2691|Compound:st077356|FD-Score:4.58|P-value:2.21E-7 ID:SGTC_2692|Compound:st077426|FD-Score:3.45|P-value:6.80E-5 ID:SGTC_2797|Compound:7859098|FD-Score:3.14|P-value:2.53E-4 ID:SGTC_2919|Compound:7978188|FD-Score:2.79|P-value:9.86E-4 ID:SGTC_3024|Compound:9090631|FD-Score:4.50|P-value:3.45E-7 ID:SGTC_3039|Compound:9090270|FD-Score:2.81|P-value:9.05E-4 ID:SGTC_3042|Compound:9091578|FD-Score:3.20|P-value:1.98E-4 ID:SGTC_3305|Compound:9127027|FD-Score:2.85|P-value:7.66E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.32|P-value:1.20E-4 ID:SGTC_11|Compound:0438-0307|FD-Score:9.68|P-value:1.17E-26 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.51|P-value:7.61E-5 ID:SGTC_208|Compound:k072-0098|FD-Score:-3.02|P-value:5.70E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:4.64|P-value:1.57E-7 ID:SGTC_227|Compound:fluconazole|FD-Score:6.17|P-value:5.42E-12 ID:SGTC_256|Compound:clotrimazole|FD-Score:5.28|P-value:2.91E-9 ID:SGTC_268|Compound:camptothecin|FD-Score:-3.11|P-value:4.09E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:3.10|P-value:2.99E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.09|P-value:3.06E-4 ID:SGTC_548|Compound:1436-0017|FD-Score:-3.39|P-value:1.26E-4 ID:SGTC_555|Compound:4296-0685|FD-Score:-3.29|P-value:1.97E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:-3.32|P-value:1.69E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:6.75|P-value:5.46E-14 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.05|P-value:5.12E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:4.72|P-value:9.65E-8 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.67|P-value:3.73E-5 ID:SGTC_873|Compound:0591-4978|FD-Score:2.83|P-value:8.52E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-3.09|P-value:4.36E-4 ID:SGTC_959|Compound:1222-0044|FD-Score:3.13|P-value:2.58E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:7.75|P-value:8.02E-18 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.13|P-value:3.66E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.14|P-value:2.46E-6 ID:SGTC_1507|Compound:4075-3230|FD-Score:3.05|P-value:3.62E-4 ID:SGTC_1698|Compound:5-fluorocytosine|FD-Score:3.68|P-value:2.37E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:12.10|P-value:2.88E-40 ID:SGTC_2010|Compound:4012422|FD-Score:4.56|P-value:2.37E-7 ID:SGTC_2030|Compound:5175110|FD-Score:3.38|P-value:9.16E-5 ID:SGTC_2273|Compound:7815444|FD-Score:3.24|P-value:1.69E-4 ID:SGTC_2285|Compound:7945142|FD-Score:7.17|P-value:1.62E-15 ID:SGTC_2307|Compound:7538926|FD-Score:5.35|P-value:1.82E-9 ID:SGTC_2314|Compound:7727897|FD-Score:-3.35|P-value:1.54E-4 ID:SGTC_2322|Compound:7413319|FD-Score:3.75|P-value:1.70E-5 ID:SGTC_2398|Compound:5228213|FD-Score:2.98|P-value:4.66E-4 ID:SGTC_2691|Compound:st077356|FD-Score:4.58|P-value:2.21E-7 ID:SGTC_2692|Compound:st077426|FD-Score:3.45|P-value:6.80E-5 ID:SGTC_2797|Compound:7859098|FD-Score:3.14|P-value:2.53E-4 ID:SGTC_2919|Compound:7978188|FD-Score:2.79|P-value:9.86E-4 ID:SGTC_3024|Compound:9090631|FD-Score:4.50|P-value:3.45E-7 ID:SGTC_3039|Compound:9090270|FD-Score:2.81|P-value:9.05E-4 ID:SGTC_3042|Compound:9091578|FD-Score:3.20|P-value:1.98E-4 ID:SGTC_3305|Compound:9127027|FD-Score:2.85|P-value:7.66E-4 ID:SGTC_3306|Compound:9127676|FD-Score:3.32|P-value:1.20E-4

Top fitness defect scores for YHR042W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1718 roemerine 12.10 11.9 µM 2.88E-40 redox potentiating 3 8.38E-34 0.33
2 SGTC_11 0438-0307 9.68 1.3 µM 1.17E-26 1 1.93E-22 5.03
3 SGTC_1083 didemethylchlorpromazine sulfoxide 7.75 707.4 nM 8.02E-18 redox potentiating 9 4.57E-15 0.13
4 SGTC_2285 7945142 7.17 200.0 µM 1.62E-15 3 3.85E-13 0.99
5 SGTC_610 4215-0184 6.75 155.0 µM 5.46E-14 exosome 9 7.23E-12 0.00
6 SGTC_227 fluconazole 6.17 33.5 µM 5.42E-12 azole & statin 28 3.36E-10 0.19
7 SGTC_2307 7538926 5.35 6.3 µM 1.82E-9 11 4.30E-8 0.12
8 SGTC_256 clotrimazole 5.28 625.0 nM 2.91E-9 ergosterol depletion effects on membrane 12 6.36E-8 0.52
9 SGTC_617 3770-0098 4.72 35.0 µM 9.65E-8 exosome 20 1.18E-6 0.15
10 SGTC_217 ketoconazole 4.64 989.0 nM 1.57E-7 ergosterol depletion effects on membrane 7 1.78E-6 0.01
11 SGTC_2691 st077356 4.58 60.2 µM 2.21E-7 8 2.37E-6 0.55
12 SGTC_2010 4012422 4.56 159.0 µM 2.37E-7 5 2.51E-6 0.52
13 SGTC_3024 9090631 4.50 49.5 µM 3.45E-7 2 3.44E-6 0.56
14 SGTC_1456 k015-0012 4.14 13.0 µM 2.46E-6 7 1.77E-5 0.44
15 SGTC_2322 7413319 3.75 38.2 µM 1.70E-5 ERAD & cell cycle 28 8.92E-5 0.03
16 SGTC_1698 5-fluorocytosine 3.68 1.1 µM 2.37E-5 exosome 16 1.17E-4 0.06
17 SGTC_2692 st077426 3.45 47.7 µM 6.80E-5 10 2.84E-4 0.13
18 SGTC_2030 5175110 3.38 200.0 µM 9.16E-5 Golgi 6 3.64E-4 0.13
19 SGTC_3306 9127676 3.32 59.8 µM 1.20E-4 4 4.58E-4 0.03
20 SGTC_2273 7815444 3.24 169.1 µM 1.69E-4 8 6.08E-4 0.09

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.346 5.03E-95 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.317 2.70E-79 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.294 7.67E-68 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.211 4.51E-35 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.207 1.27E-33 YCR093W CDC39 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.189 2.04E-28 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.188 3.79E-28 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.164 9.96E-22 YGR144W THI4 hom Thiazole synthase, abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
0.164 1.18E-21 YPL043W NOP4 het Nucleolar protein, essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs)
0.160 9.69E-21 YGR158C MTR3 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
0.157 5.19E-20 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.156 1.25E-19 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.131 2.91E-14 YER038C KRE29 het Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
0.131 3.03E-14 YDL224C WHI4 hom Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
0.130 4.82E-14 YCL017C NFS1 het Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria