YJL035C / TAD2

Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL035C / TAD2

Click on Significant Values for Screen Details ID:SGTC_71|Compound:0417-1750|FD-Score:-2.98|P-value:3.21E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:-3.88|P-value:4.31E-6 ID:SGTC_262|Compound:fenpropimorph|FD-Score:-3.03|P-value:2.55E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-4.13|P-value:1.09E-6 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:2.95|P-value:3.29E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.76|P-value:7.23E-6 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:-2.71|P-value:9.65E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:2.81|P-value:5.81E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-2.89|P-value:4.67E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:2.95|P-value:3.33E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:3.13|P-value:1.48E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-2.77|P-value:7.49E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.03|P-value:2.37E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:2.91|P-value:3.92E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:2.93|P-value:3.54E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.08|P-value:1.91E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:5.40|P-value:2.33E-10 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.26|P-value:8.31E-5 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.42|P-value:3.89E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.56|P-value:2.02E-5 ID:SGTC_1332|Compound:1480-0653|FD-Score:2.81|P-value:5.77E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:-2.86|P-value:5.20E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:2.72|P-value:8.21E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:2.94|P-value:3.44E-4 ID:SGTC_1734|Compound:st037168|FD-Score:2.69|P-value:9.26E-4 ID:SGTC_1871|Compound:st057448|FD-Score:2.95|P-value:3.28E-4 ID:SGTC_1961|Compound:st076550|FD-Score:2.71|P-value:8.59E-4 ID:SGTC_1970|Compound:st070122|FD-Score:2.70|P-value:9.17E-4 ID:SGTC_2086|Compound:5274770|FD-Score:-2.76|P-value:7.70E-4 ID:SGTC_2098|Compound:5376439|FD-Score:-3.45|P-value:3.81E-5 ID:SGTC_2099|Compound:5377090|FD-Score:-3.75|P-value:8.44E-6 ID:SGTC_2112|Compound:5488546|FD-Score:3.15|P-value:1.35E-4 ID:SGTC_2291|Compound:7917025|FD-Score:-2.70|P-value:9.82E-4 ID:SGTC_2304|Compound:7443016|FD-Score:-3.02|P-value:2.68E-4 ID:SGTC_2311|Compound:7694451|FD-Score:2.76|P-value:7.00E-4 ID:SGTC_2312|Compound:7706453|FD-Score:4.56|P-value:7.40E-8 ID:SGTC_2385|Compound:9074692|FD-Score:-3.13|P-value:1.66E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.52|P-value:2.45E-5 ID:SGTC_2486|Compound:5283202|FD-Score:-6.39|P-value:1.09E-13 ID:SGTC_2525|Compound:harmine|FD-Score:2.68|P-value:9.93E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.32|P-value:7.20E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.12|P-value:1.76E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.32|P-value:6.30E-5 ID:SGTC_2774|Compound:tamoxifen|FD-Score:2.72|P-value:8.34E-4 ID:SGTC_2829|Compound:7992743|FD-Score:2.72|P-value:8.26E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.75|P-value:7.37E-6 ID:SGTC_71|Compound:0417-1750|FD-Score:-2.98|P-value:3.21E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:-3.88|P-value:4.31E-6 ID:SGTC_262|Compound:fenpropimorph|FD-Score:-3.03|P-value:2.55E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-4.13|P-value:1.09E-6 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:2.95|P-value:3.29E-4 ID:SGTC_491|Compound:tetrandrine|FD-Score:3.76|P-value:7.23E-6 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:-2.71|P-value:9.65E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:2.81|P-value:5.81E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-2.89|P-value:4.67E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:2.95|P-value:3.33E-4 ID:SGTC_716|Compound:1683-5115|FD-Score:3.13|P-value:1.48E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-2.77|P-value:7.49E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.03|P-value:2.37E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:2.91|P-value:3.92E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:2.93|P-value:3.54E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.08|P-value:1.91E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:5.40|P-value:2.33E-10 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.26|P-value:8.31E-5 ID:SGTC_1267|Compound:0828-0272|FD-Score:3.42|P-value:3.89E-5 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.56|P-value:2.02E-5 ID:SGTC_1332|Compound:1480-0653|FD-Score:2.81|P-value:5.77E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:-2.86|P-value:5.20E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:2.72|P-value:8.21E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:2.94|P-value:3.44E-4 ID:SGTC_1734|Compound:st037168|FD-Score:2.69|P-value:9.26E-4 ID:SGTC_1871|Compound:st057448|FD-Score:2.95|P-value:3.28E-4 ID:SGTC_1961|Compound:st076550|FD-Score:2.71|P-value:8.59E-4 ID:SGTC_1970|Compound:st070122|FD-Score:2.70|P-value:9.17E-4 ID:SGTC_2086|Compound:5274770|FD-Score:-2.76|P-value:7.70E-4 ID:SGTC_2098|Compound:5376439|FD-Score:-3.45|P-value:3.81E-5 ID:SGTC_2099|Compound:5377090|FD-Score:-3.75|P-value:8.44E-6 ID:SGTC_2112|Compound:5488546|FD-Score:3.15|P-value:1.35E-4 ID:SGTC_2291|Compound:7917025|FD-Score:-2.70|P-value:9.82E-4 ID:SGTC_2304|Compound:7443016|FD-Score:-3.02|P-value:2.68E-4 ID:SGTC_2311|Compound:7694451|FD-Score:2.76|P-value:7.00E-4 ID:SGTC_2312|Compound:7706453|FD-Score:4.56|P-value:7.40E-8 ID:SGTC_2385|Compound:9074692|FD-Score:-3.13|P-value:1.66E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.52|P-value:2.45E-5 ID:SGTC_2486|Compound:5283202|FD-Score:-6.39|P-value:1.09E-13 ID:SGTC_2525|Compound:harmine|FD-Score:2.68|P-value:9.93E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.32|P-value:7.20E-5 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:-3.12|P-value:1.76E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.32|P-value:6.30E-5 ID:SGTC_2774|Compound:tamoxifen|FD-Score:2.72|P-value:8.34E-4 ID:SGTC_2829|Compound:7992743|FD-Score:2.72|P-value:8.26E-4 ID:SGTC_2831|Compound:7999036|FD-Score:3.75|P-value:7.37E-6

Top fitness defect scores for YJL035C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_988 1497-1057 5.40 103.0 µM 2.33E-10 1 3.27E-8 0.77
2 SGTC_2312 7706453 4.56 155.7 µM 7.40E-8 3 2.61E-6 0.05
3 SGTC_491 tetrandrine 3.76 80.3 µM 7.23E-6 6 8.58E-5 0.33
4 SGTC_2831 7999036 3.75 19.5 µM 7.37E-6 6 8.71E-5 0.10
5 SGTC_1326 1431-2094 3.56 10.8 µM 2.02E-5 tubulin folding & SWR complex 7 1.88E-4 0.35
6 SGTC_2410 aloe-emodin-8-o-glycoside 3.52 46.3 µM 2.45E-5 mitochondrial processes 12 2.18E-4 0.27
7 SGTC_1267 0828-0272 3.42 9.5 µM 3.89E-5 8 3.11E-4 0.02
8 SGTC_2605 digitonin 3.32 300.0 nM 6.30E-5 9 4.49E-4 0.09
9 SGTC_1043 0916-0009 3.26 19.3 µM 8.31E-5 13 5.55E-4 0.18
10 SGTC_2112 5488546 3.15 200.0 µM 1.35E-4 TSC3-RPN4 16 8.04E-4 0.00
11 SGTC_716 1683-5115 3.13 658.0 µM 1.48E-4 9 8.64E-4 0.01
12 SGTC_986 1493-0483 3.08 84.0 µM 1.91E-4 10 0.00105 0.07
13 SGTC_780 0109-0199 3.03 192.0 µM 2.37E-4 15 0.00124 0.04
14 SGTC_1871 st057448 2.95 67.3 µM 3.28E-4 22 0.00159 0.01
15 SGTC_437 s-farnesyl-l-cysteine methyl ester 2.95 6.7 µM 3.29E-4 6 0.00159 0.17
16 SGTC_618 k072-0232 2.95 33.2 µM 3.33E-4 60S ribosome export 18 0.00161 0.01
17 SGTC_1450 4112-4260 2.94 37.6 µM 3.44E-4 calcium & mitochondrial duress 16 0.00165 0.09
18 SGTC_907 3448-6986 2.93 75.5 µM 3.54E-4 20 0.00169 0.01
19 SGTC_808 0711-0032 2.91 135.0 µM 3.92E-4 26 0.00182 0.05
20 SGTC_1332 1480-0653 2.81 17.5 µM 5.77E-4 TSC3-RPN4 11 0.00245 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.178 3.67E-25 YHR131C_p YHR131C_p hom Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study
0.147 8.88E-18 YMR182W-A_p YMR182W-A_p hom Putative protein of unknown function
0.126 2.36E-13 YOR007C SGT2 hom Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; has similarity to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
0.116 1.77E-11 YDR504C SPG3 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.112 6.97E-11 YBR222C PCS60 hom Peroxisomal protein that binds AMP and mRNA, localizes to both the peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid, similar to E. coli long chain acyl-CoA synthetase
0.100 6.15E-9 YDL234C GYP7 hom GTPase-activating protein for yeast Rab family members including: Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking
0.100 6.85E-9 YDR134C YDR134C hom Hypothetical protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication
0.099 1.05E-8 YJR119C JHD2 hom JmjC domain family histone demethylase specific for H3-K4 (histone H3 Lys4); removes methyl groups specifically added by Set1p methyltransferase; protein levels regulated by Not4p (E3 ubiquitin ligase) polyubiquitin-mediated degradation
0.094 4.67E-8 YMR213W CEF1 het Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
0.091 1.40E-7 YBR261C TAE1 hom AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
0.087 4.89E-7 YML065W ORC1 het Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity
0.084 1.26E-6 YGR277C CAB4 het Probable pantetheine-phosphate adenylyltransferase (PPAT), which catalyzes the fourth step in the biosynthesis of coenzyme A from pantothenate; null mutant lethality is complemented by E. coli coaD (encoding PPAT); widely conserved
0.083 1.31E-6 YJL138C TIF2 hom Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF2 has a paralog, TIF1, that arose from the whole genome duplication
0.083 1.50E-6 YEL071W DLD3 hom D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
0.083 1.59E-6 YKL189W HYM1 het Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with Kic1p and Sog2p, localizes to sites of polarized growth during budding and during the mating response