YJL052W / TDH1

Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YJL052W / TDH1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.78|P-value:8.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.68|P-value:1.77E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:7.73|P-value:8.34E-11 ID:SGTC_121|Compound:0557-0495|FD-Score:4.77|P-value:3.94E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:-4.18|P-value:3.30E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:-4.51|P-value:1.17E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:-5.92|P-value:6.65E-7 ID:SGTC_221|Compound:trifluoperazine|FD-Score:16.90|P-value:2.15E-44 ID:SGTC_288|Compound:1315-0019|FD-Score:-3.86|P-value:8.26E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:7.29|P-value:8.49E-10 ID:SGTC_309|Compound:k408-0051|FD-Score:6.58|P-value:2.71E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.87|P-value:8.21E-7 ID:SGTC_313|Compound:3474-0040|FD-Score:6.20|P-value:1.49E-7 ID:SGTC_366|Compound:0181-0206|FD-Score:4.05|P-value:3.92E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:18.10|P-value:8.42E-51 ID:SGTC_449|Compound:xestospongin c|FD-Score:7.22|P-value:1.21E-9 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:4.10|P-value:3.31E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.96|P-value:6.21E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-4.18|P-value:3.32E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:4.92|P-value:2.27E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:3.80|P-value:8.09E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:4.39|P-value:1.38E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.94|P-value:6.74E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:5.09|P-value:1.23E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:3.98|P-value:4.73E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.61|P-value:6.59E-5 ID:SGTC_1194|Compound:1488-0194|FD-Score:-4.11|P-value:4.01E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.83|P-value:7.38E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:-4.97|P-value:2.53E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.97|P-value:1.89E-5 ID:SGTC_1606|Compound:st000094|FD-Score:-4.48|P-value:1.30E-4 ID:SGTC_1712|Compound:st032288|FD-Score:12.70|P-value:5.01E-26 ID:SGTC_1713|Compound:st033146|FD-Score:4.77|P-value:3.88E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.36|P-value:1.89E-4 ID:SGTC_1721|Compound:st082152|FD-Score:5.07|P-value:1.35E-5 ID:SGTC_1723|Compound:st034307|FD-Score:-3.85|P-value:8.50E-4 ID:SGTC_1754|Compound:st042065|FD-Score:5.52|P-value:2.47E-6 ID:SGTC_1760|Compound:st045453|FD-Score:7.19|P-value:1.41E-9 ID:SGTC_1781|Compound:st038725|FD-Score:8.48|P-value:1.25E-12 ID:SGTC_1800|Compound:st050079|FD-Score:9.40|P-value:4.30E-15 ID:SGTC_1801|Compound:st050089|FD-Score:8.17|P-value:7.54E-12 ID:SGTC_1803|Compound:st050068|FD-Score:12.60|P-value:1.64E-25 ID:SGTC_1818|Compound:st052136|FD-Score:5.17|P-value:9.26E-6 ID:SGTC_1911|Compound:st060208|FD-Score:5.49|P-value:2.81E-6 ID:SGTC_1991|Compound:st073000|FD-Score:6.26|P-value:1.14E-7 ID:SGTC_1993|Compound:st073995|FD-Score:3.74|P-value:9.44E-4 ID:SGTC_2072|Compound:5231554|FD-Score:4.38|P-value:1.39E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-4.04|P-value:4.99E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-6.96|P-value:6.29E-9 ID:SGTC_2330|Compound:7979373|FD-Score:5.77|P-value:9.02E-7 ID:SGTC_2333|Compound:9017922|FD-Score:5.88|P-value:5.65E-7 ID:SGTC_2371|Compound:9071574|FD-Score:3.77|P-value:8.74E-4 ID:SGTC_2464|Compound:5717415|FD-Score:3.75|P-value:9.37E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.71|P-value:1.15E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.94|P-value:6.65E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.74|P-value:1.27E-8 ID:SGTC_2777|Compound:climbazole|FD-Score:4.05|P-value:3.92E-4 ID:SGTC_3037|Compound:9091026|FD-Score:6.27|P-value:1.10E-7 ID:SGTC_3152|Compound:9099253|FD-Score:3.78|P-value:8.52E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.78|P-value:8.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.68|P-value:1.77E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:7.73|P-value:8.34E-11 ID:SGTC_121|Compound:0557-0495|FD-Score:4.77|P-value:3.94E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:-4.18|P-value:3.30E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:-4.51|P-value:1.17E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:-5.92|P-value:6.65E-7 ID:SGTC_221|Compound:trifluoperazine|FD-Score:16.90|P-value:2.15E-44 ID:SGTC_288|Compound:1315-0019|FD-Score:-3.86|P-value:8.26E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:7.29|P-value:8.49E-10 ID:SGTC_309|Compound:k408-0051|FD-Score:6.58|P-value:2.71E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.87|P-value:8.21E-7 ID:SGTC_313|Compound:3474-0040|FD-Score:6.20|P-value:1.49E-7 ID:SGTC_366|Compound:0181-0206|FD-Score:4.05|P-value:3.92E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:18.10|P-value:8.42E-51 ID:SGTC_449|Compound:xestospongin c|FD-Score:7.22|P-value:1.21E-9 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:4.10|P-value:3.31E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.96|P-value:6.21E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:-4.18|P-value:3.32E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:4.92|P-value:2.27E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:3.80|P-value:8.09E-4 ID:SGTC_644|Compound:1498-1197|FD-Score:4.39|P-value:1.38E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.94|P-value:6.74E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:5.09|P-value:1.23E-5 ID:SGTC_940|Compound:3232-1287|FD-Score:3.98|P-value:4.73E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.61|P-value:6.59E-5 ID:SGTC_1194|Compound:1488-0194|FD-Score:-4.11|P-value:4.01E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.83|P-value:7.38E-4 ID:SGTC_1211|Compound:1056-0033|FD-Score:-4.97|P-value:2.53E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.97|P-value:1.89E-5 ID:SGTC_1606|Compound:st000094|FD-Score:-4.48|P-value:1.30E-4 ID:SGTC_1712|Compound:st032288|FD-Score:12.70|P-value:5.01E-26 ID:SGTC_1713|Compound:st033146|FD-Score:4.77|P-value:3.88E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.36|P-value:1.89E-4 ID:SGTC_1721|Compound:st082152|FD-Score:5.07|P-value:1.35E-5 ID:SGTC_1723|Compound:st034307|FD-Score:-3.85|P-value:8.50E-4 ID:SGTC_1754|Compound:st042065|FD-Score:5.52|P-value:2.47E-6 ID:SGTC_1760|Compound:st045453|FD-Score:7.19|P-value:1.41E-9 ID:SGTC_1781|Compound:st038725|FD-Score:8.48|P-value:1.25E-12 ID:SGTC_1800|Compound:st050079|FD-Score:9.40|P-value:4.30E-15 ID:SGTC_1801|Compound:st050089|FD-Score:8.17|P-value:7.54E-12 ID:SGTC_1803|Compound:st050068|FD-Score:12.60|P-value:1.64E-25 ID:SGTC_1818|Compound:st052136|FD-Score:5.17|P-value:9.26E-6 ID:SGTC_1911|Compound:st060208|FD-Score:5.49|P-value:2.81E-6 ID:SGTC_1991|Compound:st073000|FD-Score:6.26|P-value:1.14E-7 ID:SGTC_1993|Compound:st073995|FD-Score:3.74|P-value:9.44E-4 ID:SGTC_2072|Compound:5231554|FD-Score:4.38|P-value:1.39E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-4.04|P-value:4.99E-4 ID:SGTC_2329|Compound:6138490|FD-Score:-6.96|P-value:6.29E-9 ID:SGTC_2330|Compound:7979373|FD-Score:5.77|P-value:9.02E-7 ID:SGTC_2333|Compound:9017922|FD-Score:5.88|P-value:5.65E-7 ID:SGTC_2371|Compound:9071574|FD-Score:3.77|P-value:8.74E-4 ID:SGTC_2464|Compound:5717415|FD-Score:3.75|P-value:9.37E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.71|P-value:1.15E-6 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.94|P-value:6.65E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.74|P-value:1.27E-8 ID:SGTC_2777|Compound:climbazole|FD-Score:4.05|P-value:3.92E-4 ID:SGTC_3037|Compound:9091026|FD-Score:6.27|P-value:1.10E-7 ID:SGTC_3152|Compound:9099253|FD-Score:3.78|P-value:8.52E-4

Top fitness defect scores for YJL052W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_439 amiodarone 18.10 34.4 µM 8.42E-51 NEO1-PIK1 1 7.68E-74
2 SGTC_221 trifluoperazine 16.90 10.3 µM 2.15E-44 NEO1-PIK1 17 2.13E-64
3 SGTC_1712 st032288 12.70 53.4 µM 5.01E-26 calcium & mitochondrial duress 6 2.38E-37
4 SGTC_1803 st050068 12.60 52.4 µM 1.64E-25 NEO1-PIK1 9 1.37E-36
5 SGTC_1800 st050079 9.40 29.1 µM 4.30E-15 NEO1-PIK1 7 2.75E-21
6 SGTC_1781 st038725 8.48 66.3 µM 1.25E-12 NEO1 28 1.12E-17
7 SGTC_1801 st050089 8.17 28.4 µM 7.54E-12 NEO1-PIK1 25 1.55E-16
8 SGTC_117 lucigenin 7.73 22.1 µM 8.34E-11 redox potentiating 19 5.21E-15
9 SGTC_301 4119-0100 7.29 24.5 µM 8.49E-10 NEO1 26 1.55E-13
10 SGTC_449 xestospongin c 7.22 11.2 µM 1.21E-9 NEO1-PIK1 9 2.61E-13
11 SGTC_1760 st045453 7.19 10.4 µM 1.41E-9 NEO1-PIK1 67 3.26E-13
12 SGTC_2688 kanamycin b 6.74 96.7 µM 1.27E-8 calcium & mitochondrial duress 117 8.01E-12
13 SGTC_309 k408-0051 6.58 1.5 µM 2.71E-8 10 2.41E-11
14 SGTC_3037 9091026 6.27 49.5 µM 1.10E-7 NEO1-PIK1 42 1.85E-10
15 SGTC_1991 st073000 6.26 8.9 µM 1.14E-7 NEO1 28 1.96E-10
16 SGTC_313 3474-0040 6.20 5.0 µM 1.49E-7 NEO1-PIK1 22 2.90E-10
17 SGTC_2333 9017922 5.88 200.0 µM 5.65E-7 8 2.01E-9
18 SGTC_2330 7979373 5.77 200.0 µM 9.02E-7 60S ribosome export 12 3.97E-9
19 SGTC_2582 agaric acid 5.71 100.0 µM 1.15E-6 redox potentiating 176 5.64E-9
20 SGTC_1754 st042065 5.52 16.1 µM 2.47E-6 NEO1-PIK1 107 1.71E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.452 1.72E-168 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.416 3.10E-140 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.413 1.98E-138 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.404 1.04E-131 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.399 3.05E-128 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.395 1.97E-125 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.387 2.58E-120 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.387 9.55E-120 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.380 9.17E-116 YOR039W CKB2 hom Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.374 6.97E-112 YIL048W NEO1 het Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.364 9.51E-106 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.363 8.80E-105 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.352 2.70E-98 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.345 4.12E-94 YML094C-A_d YML094C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
0.342 1.25E-92 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex