YJL076W / NET1

Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication

Zygosity: Heterozygous strain
fixedexpanded
Profile for YJL076W / NET1

Click on Significant Values for Screen Details ID:SGTC_66|Compound:0109-0187|FD-Score:3.79|P-value:9.06E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:3.49|P-value:2.85E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.74|P-value:1.07E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.46|P-value:3.09E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.19|P-value:8.24E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.47|P-value:2.96E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.59|P-value:1.91E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:5.72|P-value:7.35E-9 ID:SGTC_594|Compound:1487-1584|FD-Score:3.68|P-value:1.39E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:3.75|P-value:1.05E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:4.49|P-value:4.49E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.22|P-value:7.34E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:3.61|P-value:1.79E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.65|P-value:1.53E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-3.90|P-value:4.77E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:3.47|P-value:3.03E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:3.21|P-value:7.48E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.16|P-value:8.88E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:4.57|P-value:2.98E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.74|P-value:9.09E-5 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.80|P-value:8.46E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.46|P-value:3.10E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.29|P-value:4.93E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.64|P-value:1.60E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:4.06|P-value:2.94E-5 ID:SGTC_1389|Compound:3405-0187|FD-Score:3.23|P-value:7.10E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-4.01|P-value:3.05E-5 ID:SGTC_1516|Compound:st013856|FD-Score:6.09|P-value:7.99E-10 ID:SGTC_1616|Compound:st002734|FD-Score:3.26|P-value:6.40E-4 ID:SGTC_1650|Compound:st011916|FD-Score:3.68|P-value:1.37E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.40|P-value:3.28E-4 ID:SGTC_1766|Compound:st045414|FD-Score:4.31|P-value:1.00E-5 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.59|P-value:1.93E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-3.36|P-value:3.84E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-4.94|P-value:3.88E-7 ID:SGTC_2172|Compound:5754775|FD-Score:-3.22|P-value:6.26E-4 ID:SGTC_2236|Compound:dapsone|FD-Score:3.26|P-value:6.36E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.55|P-value:2.24E-4 ID:SGTC_2312|Compound:7706453|FD-Score:3.39|P-value:3.99E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.82|P-value:7.92E-5 ID:SGTC_2458|Compound:5808907|FD-Score:4.09|P-value:2.58E-5 ID:SGTC_2482|Compound:5981709|FD-Score:3.34|P-value:4.83E-4 ID:SGTC_2483|Compound:5729050|FD-Score:-3.60|P-value:1.55E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.75|P-value:1.05E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.17|P-value:8.78E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.38|P-value:4.16E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.60|P-value:1.54E-4 ID:SGTC_2844|Compound:9010103|FD-Score:-4.57|P-value:2.49E-6 ID:SGTC_2846|Compound:9008642|FD-Score:3.96|P-value:4.50E-5 ID:SGTC_2906|Compound:7926292|FD-Score:-3.25|P-value:5.68E-4 ID:SGTC_2988|Compound:9073640|FD-Score:6.19|P-value:4.28E-10 ID:SGTC_3078|Compound:9116413|FD-Score:3.81|P-value:8.28E-5 ID:SGTC_3103|Compound:9120822|FD-Score:-3.84|P-value:6.15E-5 ID:SGTC_3140|Compound:9094243|FD-Score:3.14|P-value:9.69E-4 ID:SGTC_3189|Compound:9110682|FD-Score:4.43|P-value:5.75E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:3.79|P-value:9.06E-5 ID:SGTC_129|Compound:0088-0017|FD-Score:3.49|P-value:2.85E-4 ID:SGTC_285|Compound:1486-1325|FD-Score:3.74|P-value:1.07E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:3.46|P-value:3.09E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:3.19|P-value:8.24E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.47|P-value:2.96E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.59|P-value:1.91E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:5.72|P-value:7.35E-9 ID:SGTC_594|Compound:1487-1584|FD-Score:3.68|P-value:1.39E-4 ID:SGTC_599|Compound:1935-0052|FD-Score:3.75|P-value:1.05E-4 ID:SGTC_638|Compound:3970-0837|FD-Score:4.49|P-value:4.49E-6 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.22|P-value:7.34E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:3.61|P-value:1.79E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.65|P-value:1.53E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-3.90|P-value:4.77E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:3.47|P-value:3.03E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:3.21|P-value:7.48E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.16|P-value:8.88E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:4.57|P-value:2.98E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.74|P-value:9.09E-5 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.80|P-value:8.46E-5 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:3.46|P-value:3.10E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.29|P-value:4.93E-4 ID:SGTC_1324|Compound:1418-0026|FD-Score:3.64|P-value:1.60E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:4.06|P-value:2.94E-5 ID:SGTC_1389|Compound:3405-0187|FD-Score:3.23|P-value:7.10E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-4.01|P-value:3.05E-5 ID:SGTC_1516|Compound:st013856|FD-Score:6.09|P-value:7.99E-10 ID:SGTC_1616|Compound:st002734|FD-Score:3.26|P-value:6.40E-4 ID:SGTC_1650|Compound:st011916|FD-Score:3.68|P-value:1.37E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.40|P-value:3.28E-4 ID:SGTC_1766|Compound:st045414|FD-Score:4.31|P-value:1.00E-5 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.59|P-value:1.93E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-3.36|P-value:3.84E-4 ID:SGTC_1944|Compound:st076371|FD-Score:-4.94|P-value:3.88E-7 ID:SGTC_2172|Compound:5754775|FD-Score:-3.22|P-value:6.26E-4 ID:SGTC_2236|Compound:dapsone|FD-Score:3.26|P-value:6.36E-4 ID:SGTC_2311|Compound:7694451|FD-Score:3.55|P-value:2.24E-4 ID:SGTC_2312|Compound:7706453|FD-Score:3.39|P-value:3.99E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.82|P-value:7.92E-5 ID:SGTC_2458|Compound:5808907|FD-Score:4.09|P-value:2.58E-5 ID:SGTC_2482|Compound:5981709|FD-Score:3.34|P-value:4.83E-4 ID:SGTC_2483|Compound:5729050|FD-Score:-3.60|P-value:1.55E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.75|P-value:1.05E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:3.17|P-value:8.78E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.38|P-value:4.16E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.60|P-value:1.54E-4 ID:SGTC_2844|Compound:9010103|FD-Score:-4.57|P-value:2.49E-6 ID:SGTC_2846|Compound:9008642|FD-Score:3.96|P-value:4.50E-5 ID:SGTC_2906|Compound:7926292|FD-Score:-3.25|P-value:5.68E-4 ID:SGTC_2988|Compound:9073640|FD-Score:6.19|P-value:4.28E-10 ID:SGTC_3078|Compound:9116413|FD-Score:3.81|P-value:8.28E-5 ID:SGTC_3103|Compound:9120822|FD-Score:-3.84|P-value:6.15E-5 ID:SGTC_3140|Compound:9094243|FD-Score:3.14|P-value:9.69E-4 ID:SGTC_3189|Compound:9110682|FD-Score:4.43|P-value:5.75E-6

Top fitness defect scores for YJL076W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2988 9073640 6.19 71.4 µM 4.28E-10 3 3.05E-10 0.38
2 SGTC_1516 st013856 6.09 110.0 µM 7.99E-10 1 5.75E-10 1.43
3 SGTC_542 3381-0244 5.72 89.3 µM 7.35E-9 6 5.45E-9 1.48
4 SGTC_1014 4130-3005 4.57 69.3 µM 2.98E-6 3 2.40E-6 0.40
5 SGTC_638 3970-0837 4.49 4.1 µM 4.49E-6 2 3.64E-6 0.32
6 SGTC_3189 9110682 4.43 49.5 µM 5.75E-6 7 4.68E-6 0.62
7 SGTC_1766 st045414 4.31 44.8 µM 1.00E-5 RPP1 & pyrimidine depletion 10 8.20E-6 0.11
8 SGTC_2458 5808907 4.09 16.4 µM 2.58E-5 3 2.15E-5 0.32
9 SGTC_1346 1488-2224 4.06 62.4 µM 2.94E-5 10 2.45E-5 0.02
10 SGTC_2846 9008642 3.96 19.5 µM 4.50E-5 6 3.77E-5 0.01
11 SGTC_2401 5128683 3.82 200.0 µM 7.92E-5 9 6.70E-5 0.03
12 SGTC_3078 9116413 3.81 49.5 µM 8.28E-5 unfolded protein response 6 7.01E-5 0.04
13 SGTC_1090 efavirenz 3.80 20.7 µM 8.46E-5 4 7.16E-5 0.07
14 SGTC_66 0109-0187 3.79 42.4 µM 9.06E-5 6 7.68E-5 0.07
15 SGTC_2488 5268135 3.75 45.7 µM 1.05E-4 9 8.94E-5 0.14
16 SGTC_599 1935-0052 3.75 86.5 µM 1.05E-4 5 8.95E-5 0.19
17 SGTC_285 1486-1325 3.74 46.0 µM 1.07E-4 6 9.07E-5 0.03
18 SGTC_1650 st011916 3.68 45.8 µM 1.37E-4 3 1.17E-4 0.23
19 SGTC_594 1487-1584 3.68 10.8 µM 1.39E-4 12 1.19E-4 0.18
20 SGTC_695 3505-6186 3.65 243.0 µM 1.53E-4 9 1.31E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.130 3.86E-14 YLR310C CDC25 het Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
0.096 2.32E-8 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.092 8.35E-8 YCL043C PDI1 het Protein disulfide isomerase; multifunctional protein of ER lumen, essential for formation of disulfide bonds in secretory and cell-surface proteins, unscrambles non-native disulfide bonds; key regulator of Ero1p; forms complex with Mnl1p that has exomannosidase activity, processing unfolded protein-bound Man8GlcNAc2 oligosaccharides to Man7GlcNAc2, promoting degradation in unfolded protein response; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
0.088 3.16E-7 YDL210W UGA4 hom Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
0.088 3.57E-7 YER020W GPA2 hom Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.086 6.22E-7 YPR131C NAT3 hom Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
0.086 6.82E-7 YLL013C PUF3 hom Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins
0.083 1.48E-6 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.081 2.29E-6 YDR094W_d YDR094W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2
0.081 2.79E-6 YPL174C NIP100 hom Large subunit of the dynactin complex, which is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
0.078 5.71E-6 YNL062C GCD10 het Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
0.078 6.40E-6 YDL153C SAS10 het Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
0.075 1.24E-5 YNL016W PUB1 hom Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress
0.074 1.91E-5 YLR111W_d YLR111W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.074 1.94E-5 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data