HIPHOP chemogenomics database

YJL101C / GSH1

Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YJL101C / GSH1

Click on Significant Values for Screen Details

Top fitness defect scores for YJL101C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_168	3970-0862	22.10	530.5 µM	2.68E-123		1	6.39E-109
2	SGTC_2405	ticlatone	11.40	3.5 µM	3.18E-34		8	2.21E-30
3	SGTC_150	3970-0969	9.73	77.2 µM	1.79E-25		3	1.16E-22
4	SGTC_184	1494-0393	9.21	24.7 µM	4.70E-23		8	1.58E-20
5	SGTC_398	bronopol	7.53	33.3 µM	4.97E-16	RNA processing & uracil transport	5	2.49E-14
6	SGTC_3210	9128157	7.00	49.5 µM	4.37E-14		9	1.30E-12
7	SGTC_2580	diallyl disulphide	6.57	81.4 µM	1.22E-12		4	2.45E-11
8	SGTC_1141	3253-0389	5.94	52.6 µM	1.21E-10		1	1.42E-9
9	SGTC_2200	6600604	5.86	21.9 µM	2.11E-10	tubulin folding & SWR complex	31	2.33E-9
10	SGTC_3037	9091026	5.66	49.5 µM	7.84E-10	NEO1-PIK1	57	7.42E-9
11	SGTC_695	3505-6186	5.00	243.0 µM	4.90E-8		10	2.87E-7
12	SGTC_2751	anetholtrithion	4.94	17.2 µM	6.88E-8		12	3.87E-7
13	SGTC_163	1327-0036	4.92	7.5 µM	7.74E-8		65	4.30E-7
14	SGTC_1700	st030840	4.58	7.9 µM	5.32E-7	PDR1	17	2.37E-6
15	SGTC_778	3456-2434	4.41	196.0 µM	1.30E-6	mitochondrial response to ROS	62	5.23E-6
16	SGTC_1275	0867-0008	4.22	21.1 µM	3.39E-6		10	1.22E-5
17	SGTC_3162	9099653	4.14	49.5 µM	5.12E-6		3	1.76E-5
18	SGTC_3059	1327-0034	4.02	3.0 µM	8.85E-6		51	2.85E-5
19	SGTC_984	1492-0255	4.02	2.9 µM	8.90E-6	SWF1 & branched chain AA biosynthesis	26	2.87E-5
20	SGTC_523	1934-0222	3.94	13.7 µM	1.32E-5		31	4.06E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.307	3.41E-74	YOL049W	GSH2	hom	Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
0.278	2.82E-60	YDR005C	MAF1	hom	Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.247	1.09E-47	YOR282W_d	YOR282W_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.233	2.57E-42	YDR069C	DOA4	hom	Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.231	1.07E-41	YFL033C	RIM15	hom	Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.223	6.56E-39	YHR205W	SCH9	hom	AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.222	1.17E-38	YJL054W	TIM54	het	Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.220	6.63E-38	YDR088C	SLU7	het	RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.214	7.86E-36	YIR008C	PRI1	het	Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.201	6.40E-32	YJL019W	MPS3	het	Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.200	1.41E-31	YDR002W	YRB1	het	Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
0.200	2.26E-31	YJR074W	MOG1	hom	Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p
0.195	4.24E-30	YKL028W	TFA1	het	TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
0.194	1.26E-29	YML073C	RPL6A	hom	Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
0.179	2.18E-25	YJR013W	GPI14	het	Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M