YJR125C / ENT3

Protein containing an N-terminal epsin-like domain involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p

Zygosity: Homozygous strain
fixedexpanded
Profile for YJR125C / ENT3

Click on Significant Values for Screen Details ID:SGTC_77|Compound:0828-0286|FD-Score:3.58|P-value:2.09E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:4.91|P-value:6.77E-7 ID:SGTC_132|Compound:0417-1715|FD-Score:4.56|P-value:3.60E-6 ID:SGTC_140|Compound:0062-0056|FD-Score:-4.02|P-value:4.48E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:3.76|P-value:1.03E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.40|P-value:4.03E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:4.28|P-value:1.24E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.55|P-value:2.32E-8 ID:SGTC_480|Compound:am 92016|FD-Score:3.71|P-value:1.27E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:5.29|P-value:9.81E-8 ID:SGTC_570|Compound:0987-0065|FD-Score:3.54|P-value:2.36E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:4.76|P-value:1.39E-6 ID:SGTC_849|Compound:0386-0493|FD-Score:4.18|P-value:1.91E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:-3.44|P-value:4.09E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.18|P-value:8.39E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.51|P-value:2.64E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.37|P-value:4.43E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.44|P-value:3.41E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.77|P-value:9.95E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.92|P-value:5.64E-5 ID:SGTC_1800|Compound:st050079|FD-Score:4.21|P-value:1.70E-5 ID:SGTC_1803|Compound:st050068|FD-Score:4.48|P-value:5.12E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.03|P-value:3.55E-5 ID:SGTC_2051|Compound:5263410|FD-Score:3.46|P-value:3.23E-4 ID:SGTC_2057|Compound:5236571|FD-Score:5.46|P-value:3.92E-8 ID:SGTC_2065|Compound:5221596|FD-Score:-3.56|P-value:2.66E-4 ID:SGTC_2066|Compound:5222451|FD-Score:4.32|P-value:1.06E-5 ID:SGTC_2069|Compound:5228469|FD-Score:3.64|P-value:1.67E-4 ID:SGTC_2082|Compound:5219693|FD-Score:-3.49|P-value:3.42E-4 ID:SGTC_2109|Compound:5483026|FD-Score:5.43|P-value:4.65E-8 ID:SGTC_2315|Compound:7733312|FD-Score:-3.24|P-value:8.09E-4 ID:SGTC_2343|Compound:7990716|FD-Score:3.18|P-value:8.58E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.23|P-value:7.16E-4 ID:SGTC_2414|Compound:st077232|FD-Score:3.94|P-value:5.09E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:5.79|P-value:6.30E-9 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:4.11|P-value:2.54E-5 ID:SGTC_2655|Compound:perillic acid|FD-Score:4.39|P-value:7.62E-6 ID:SGTC_2668|Compound:2',4'-dihydroxychalcone|FD-Score:3.76|P-value:1.03E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.49|P-value:2.90E-4 ID:SGTC_2793|Compound:7325131|FD-Score:3.48|P-value:2.93E-4 ID:SGTC_2819|Compound:7996655|FD-Score:-3.99|P-value:5.22E-5 ID:SGTC_2917|Compound:7979103|FD-Score:4.02|P-value:3.75E-5 ID:SGTC_3015|Compound:9080845|FD-Score:3.30|P-value:5.59E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.21|P-value:7.67E-4 ID:SGTC_3165|Compound:9101544|FD-Score:3.91|P-value:5.80E-5 ID:SGTC_3190|Compound:9110366|FD-Score:-3.85|P-value:8.91E-5 ID:SGTC_3195|Compound:9113082|FD-Score:-3.22|P-value:8.77E-4 ID:SGTC_3260|Compound:9137109|FD-Score:-3.64|P-value:1.96E-4 ID:SGTC_3267|Compound:9137951|FD-Score:3.58|P-value:2.10E-4 ID:SGTC_3297|Compound:9117502|FD-Score:-3.43|P-value:4.27E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:3.58|P-value:2.09E-4 ID:SGTC_91|Compound:0417-1751|FD-Score:4.91|P-value:6.77E-7 ID:SGTC_132|Compound:0417-1715|FD-Score:4.56|P-value:3.60E-6 ID:SGTC_140|Compound:0062-0056|FD-Score:-4.02|P-value:4.48E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:3.76|P-value:1.03E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.40|P-value:4.03E-4 ID:SGTC_301|Compound:4119-0100|FD-Score:4.28|P-value:1.24E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.55|P-value:2.32E-8 ID:SGTC_480|Compound:am 92016|FD-Score:3.71|P-value:1.27E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:5.29|P-value:9.81E-8 ID:SGTC_570|Compound:0987-0065|FD-Score:3.54|P-value:2.36E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:4.76|P-value:1.39E-6 ID:SGTC_849|Compound:0386-0493|FD-Score:4.18|P-value:1.91E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:-3.44|P-value:4.09E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.18|P-value:8.39E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:3.51|P-value:2.64E-4 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.37|P-value:4.43E-4 ID:SGTC_1226|Compound:3909-7979|FD-Score:3.44|P-value:3.41E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.77|P-value:9.95E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.92|P-value:5.64E-5 ID:SGTC_1800|Compound:st050079|FD-Score:4.21|P-value:1.70E-5 ID:SGTC_1803|Compound:st050068|FD-Score:4.48|P-value:5.12E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.03|P-value:3.55E-5 ID:SGTC_2051|Compound:5263410|FD-Score:3.46|P-value:3.23E-4 ID:SGTC_2057|Compound:5236571|FD-Score:5.46|P-value:3.92E-8 ID:SGTC_2065|Compound:5221596|FD-Score:-3.56|P-value:2.66E-4 ID:SGTC_2066|Compound:5222451|FD-Score:4.32|P-value:1.06E-5 ID:SGTC_2069|Compound:5228469|FD-Score:3.64|P-value:1.67E-4 ID:SGTC_2082|Compound:5219693|FD-Score:-3.49|P-value:3.42E-4 ID:SGTC_2109|Compound:5483026|FD-Score:5.43|P-value:4.65E-8 ID:SGTC_2315|Compound:7733312|FD-Score:-3.24|P-value:8.09E-4 ID:SGTC_2343|Compound:7990716|FD-Score:3.18|P-value:8.58E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.23|P-value:7.16E-4 ID:SGTC_2414|Compound:st077232|FD-Score:3.94|P-value:5.09E-5 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:5.79|P-value:6.30E-9 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:4.11|P-value:2.54E-5 ID:SGTC_2655|Compound:perillic acid|FD-Score:4.39|P-value:7.62E-6 ID:SGTC_2668|Compound:2',4'-dihydroxychalcone|FD-Score:3.76|P-value:1.03E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.49|P-value:2.90E-4 ID:SGTC_2793|Compound:7325131|FD-Score:3.48|P-value:2.93E-4 ID:SGTC_2819|Compound:7996655|FD-Score:-3.99|P-value:5.22E-5 ID:SGTC_2917|Compound:7979103|FD-Score:4.02|P-value:3.75E-5 ID:SGTC_3015|Compound:9080845|FD-Score:3.30|P-value:5.59E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.21|P-value:7.67E-4 ID:SGTC_3165|Compound:9101544|FD-Score:3.91|P-value:5.80E-5 ID:SGTC_3190|Compound:9110366|FD-Score:-3.85|P-value:8.91E-5 ID:SGTC_3195|Compound:9113082|FD-Score:-3.22|P-value:8.77E-4 ID:SGTC_3260|Compound:9137109|FD-Score:-3.64|P-value:1.96E-4 ID:SGTC_3267|Compound:9137951|FD-Score:3.58|P-value:2.10E-4 ID:SGTC_3297|Compound:9117502|FD-Score:-3.43|P-value:4.27E-4

Top fitness defect scores for YJR125C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2615 purpurogallin-4-carboxylic acid 5.79 100.0 µM 6.30E-9 redox potentiating 27 3.61E-9
2 SGTC_445 e6 berbamine 5.55 66.0 µM 2.32E-8 NEO1-PIK1 38 1.40E-8
3 SGTC_2057 5236571 5.46 38.3 µM 3.92E-8 RPP1 & pyrimidine depletion 8 2.40E-8
4 SGTC_2109 5483026 5.43 200.0 µM 4.65E-8 RSC complex & mRNA processing 7 2.87E-8
5 SGTC_538 2922-0838 5.29 53.3 µM 9.81E-8 Golgi 10 6.21E-8
6 SGTC_91 0417-1751 4.91 58.4 µM 6.77E-7 Golgi 27 4.60E-7
7 SGTC_745 0469-0705 4.76 296.0 µM 1.39E-6 mitochondrial processes 10 9.72E-7
8 SGTC_132 0417-1715 4.56 25.8 µM 3.60E-6 RPP1 & pyrimidine depletion 12 2.59E-6
9 SGTC_1803 st050068 4.48 52.4 µM 5.12E-6 NEO1-PIK1 142 3.73E-6
10 SGTC_2655 perillic acid 4.39 100.0 µM 7.62E-6 1 5.64E-6
11 SGTC_2066 5222451 4.32 176.0 µM 1.06E-5 heme biosynthesis & mitochondrial translocase 25 7.92E-6
12 SGTC_301 4119-0100 4.28 24.5 µM 1.24E-5 NEO1 73 9.30E-6
13 SGTC_1800 st050079 4.21 29.1 µM 1.70E-5 NEO1-PIK1 108 1.29E-5
14 SGTC_849 0386-0493 4.18 241.0 µM 1.91E-5 14 1.46E-5
15 SGTC_2635 hydroquinidine 4.11 100.0 µM 2.54E-5 21 1.96E-5
16 SGTC_1844 rapamycin 4.03 4.0 nM 3.55E-5 156 2.77E-5
17 SGTC_2917 7979103 4.02 53.8 µM 3.75E-5 28 2.93E-5
18 SGTC_2414 st077232 3.94 92.9 µM 5.09E-5 20 4.02E-5
19 SGTC_1754 st042065 3.92 16.1 µM 5.64E-5 NEO1-PIK1 181 4.47E-5
20 SGTC_3165 9101544 3.91 49.5 µM 5.80E-5 11 4.61E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.144 5.07E-17 YHR187W IKI1 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin
0.142 1.42E-16 YPR092W_d YPR092W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.140 3.01E-16 YLL040C VPS13 hom Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
0.133 1.26E-14 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.131 2.82E-14 YLR095C IOC2 hom Member of a complex (Isw1b) with Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing, contains a PHD finger motif
0.130 4.71E-14 YPL202C AFT2 hom Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
0.129 5.70E-14 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.127 1.57E-13 YKL145W RPT1 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
0.126 2.09E-13 YML094C-A_d YML094C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
0.122 1.46E-12 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.119 5.15E-12 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.112 7.72E-11 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.109 2.35E-10 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.108 3.22E-10 YOL146W PSF3 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.108 3.29E-10 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB