YKL054C / DEF1

RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; mutant is deficient in Zip1p loading onto chromosomes during meiosis

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL054C / DEF1

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:3.30|P-value:3.21E-4 ID:SGTC_25|Compound:0118-0223|FD-Score:3.10|P-value:6.79E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.56|P-value:1.16E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.25|P-value:3.85E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:3.97|P-value:1.99E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:5.50|P-value:5.73E-9 ID:SGTC_164|Compound:4100-3994|FD-Score:3.72|P-value:5.94E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.19|P-value:5.86E-6 ID:SGTC_536|Compound:3852-0578|FD-Score:-3.68|P-value:5.91E-5 ID:SGTC_567|Compound:1080-0542|FD-Score:-3.20|P-value:4.06E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.00|P-value:9.46E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:3.99|P-value:1.77E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:3.07|P-value:7.60E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:3.29|P-value:3.30E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.20|P-value:4.00E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.33|P-value:2.83E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.49|P-value:1.55E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.74|P-value:5.41E-5 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.94|P-value:2.30E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.71|P-value:6.19E-5 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.82|P-value:3.75E-5 ID:SGTC_1673|Compound:st016611|FD-Score:3.08|P-value:7.32E-4 ID:SGTC_1678|Compound:st019265|FD-Score:4.16|P-value:8.20E-6 ID:SGTC_1749|Compound:st037932|FD-Score:4.22|P-value:6.31E-6 ID:SGTC_1934|Compound:st074720|FD-Score:3.51|P-value:1.40E-4 ID:SGTC_2077|Compound:5212015|FD-Score:3.52|P-value:1.37E-4 ID:SGTC_2159|Compound:5658611|FD-Score:3.79|P-value:4.32E-5 ID:SGTC_2167|Compound:5876490|FD-Score:3.98|P-value:1.90E-5 ID:SGTC_2188|Compound:6126565|FD-Score:4.59|P-value:9.85E-7 ID:SGTC_2204|Compound:7233102|FD-Score:3.08|P-value:7.20E-4 ID:SGTC_2273|Compound:7815444|FD-Score:3.31|P-value:3.03E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.09|P-value:6.87E-4 ID:SGTC_2301|Compound:7769476|FD-Score:3.79|P-value:4.35E-5 ID:SGTC_2390|Compound:9097792|FD-Score:3.95|P-value:2.13E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.59|P-value:1.03E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.27|P-value:3.63E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.43|P-value:1.97E-4 ID:SGTC_2459|Compound:5811157|FD-Score:4.32|P-value:3.89E-6 ID:SGTC_2469|Compound:5655019|FD-Score:4.11|P-value:1.05E-5 ID:SGTC_2474|Compound:5321570|FD-Score:3.85|P-value:3.36E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:4.81|P-value:3.05E-7 ID:SGTC_2516|Compound:gossypol|FD-Score:-3.21|P-value:3.93E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.33|P-value:2.45E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.21|P-value:4.51E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.44|P-value:2.16E-6 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.59|P-value:1.03E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.08|P-value:7.34E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.19|P-value:4.84E-4 ID:SGTC_2836|Compound:9010338|FD-Score:-3.45|P-value:1.52E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.57|P-value:1.09E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.38|P-value:2.32E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.48|P-value:1.59E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-3.04|P-value:7.21E-4 ID:SGTC_3017|Compound:9080681|FD-Score:3.98|P-value:1.90E-5 ID:SGTC_3044|Compound:9093193|FD-Score:5.20|P-value:3.55E-8 ID:SGTC_3058|Compound:9093861|FD-Score:3.30|P-value:3.16E-4 ID:SGTC_3212|Compound:9128307|FD-Score:4.12|P-value:9.92E-6 ID:SGTC_18|Compound:0111-0177|FD-Score:3.30|P-value:3.21E-4 ID:SGTC_25|Compound:0118-0223|FD-Score:3.10|P-value:6.79E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.56|P-value:1.16E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.25|P-value:3.85E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:3.97|P-value:1.99E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:5.50|P-value:5.73E-9 ID:SGTC_164|Compound:4100-3994|FD-Score:3.72|P-value:5.94E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.19|P-value:5.86E-6 ID:SGTC_536|Compound:3852-0578|FD-Score:-3.68|P-value:5.91E-5 ID:SGTC_567|Compound:1080-0542|FD-Score:-3.20|P-value:4.06E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.00|P-value:9.46E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:3.99|P-value:1.77E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:3.07|P-value:7.60E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:3.29|P-value:3.30E-4 ID:SGTC_936|Compound:3005-4620|FD-Score:-3.20|P-value:4.00E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:3.33|P-value:2.83E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:3.49|P-value:1.55E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.74|P-value:5.41E-5 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.94|P-value:2.30E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.71|P-value:6.19E-5 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.82|P-value:3.75E-5 ID:SGTC_1673|Compound:st016611|FD-Score:3.08|P-value:7.32E-4 ID:SGTC_1678|Compound:st019265|FD-Score:4.16|P-value:8.20E-6 ID:SGTC_1749|Compound:st037932|FD-Score:4.22|P-value:6.31E-6 ID:SGTC_1934|Compound:st074720|FD-Score:3.51|P-value:1.40E-4 ID:SGTC_2077|Compound:5212015|FD-Score:3.52|P-value:1.37E-4 ID:SGTC_2159|Compound:5658611|FD-Score:3.79|P-value:4.32E-5 ID:SGTC_2167|Compound:5876490|FD-Score:3.98|P-value:1.90E-5 ID:SGTC_2188|Compound:6126565|FD-Score:4.59|P-value:9.85E-7 ID:SGTC_2204|Compound:7233102|FD-Score:3.08|P-value:7.20E-4 ID:SGTC_2273|Compound:7815444|FD-Score:3.31|P-value:3.03E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.09|P-value:6.87E-4 ID:SGTC_2301|Compound:7769476|FD-Score:3.79|P-value:4.35E-5 ID:SGTC_2390|Compound:9097792|FD-Score:3.95|P-value:2.13E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.59|P-value:1.03E-4 ID:SGTC_2450|Compound:5739737|FD-Score:3.27|P-value:3.63E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.43|P-value:1.97E-4 ID:SGTC_2459|Compound:5811157|FD-Score:4.32|P-value:3.89E-6 ID:SGTC_2469|Compound:5655019|FD-Score:4.11|P-value:1.05E-5 ID:SGTC_2474|Compound:5321570|FD-Score:3.85|P-value:3.36E-5 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:4.81|P-value:3.05E-7 ID:SGTC_2516|Compound:gossypol|FD-Score:-3.21|P-value:3.93E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.33|P-value:2.45E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.21|P-value:4.51E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.44|P-value:2.16E-6 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.59|P-value:1.03E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.08|P-value:7.34E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.19|P-value:4.84E-4 ID:SGTC_2836|Compound:9010338|FD-Score:-3.45|P-value:1.52E-4 ID:SGTC_2851|Compound:9015922|FD-Score:3.57|P-value:1.09E-4 ID:SGTC_2881|Compound:9058953|FD-Score:3.38|P-value:2.32E-4 ID:SGTC_2944|Compound:9067346|FD-Score:3.48|P-value:1.59E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-3.04|P-value:7.21E-4 ID:SGTC_3017|Compound:9080681|FD-Score:3.98|P-value:1.90E-5 ID:SGTC_3044|Compound:9093193|FD-Score:5.20|P-value:3.55E-8 ID:SGTC_3058|Compound:9093861|FD-Score:3.30|P-value:3.16E-4 ID:SGTC_3212|Compound:9128307|FD-Score:4.12|P-value:9.92E-6

Top fitness defect scores for YKL054C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_160 3453-0671 5.50 289.7 µM 5.73E-9 14 1.85E-8
2 SGTC_3044 9093193 5.20 49.5 µM 3.55E-8 6 1.01E-7
3 SGTC_2496 diffractaic acid 4.81 79.8 µM 3.05E-7 12 7.45E-7
4 SGTC_2188 6126565 4.59 140.7 µM 9.85E-7 mitochondrial processes 7 2.21E-6
5 SGTC_2636 apigenin dimethyl ether 4.44 100.0 µM 2.16E-6 30 4.59E-6
6 SGTC_2459 5811157 4.32 9.2 µM 3.89E-6 mitochondrial processes 7 7.94E-6
7 SGTC_1749 st037932 4.22 54.2 µM 6.31E-6 24 1.25E-5
8 SGTC_1678 st019265 4.16 80.2 µM 8.20E-6 60S ribosome export 15 1.59E-5
9 SGTC_3212 9128307 4.12 49.5 µM 9.92E-6 40 1.90E-5
10 SGTC_2469 5655019 4.11 90.8 µM 1.05E-5 20 2.01E-5
11 SGTC_771 0250-0039 3.99 27.3 µM 1.77E-5 13 3.26E-5
12 SGTC_2167 5876490 3.98 200.0 µM 1.90E-5 31 3.48E-5
13 SGTC_3017 9080681 3.98 49.5 µM 1.90E-5 14 3.48E-5
14 SGTC_132 0417-1715 3.97 25.8 µM 1.99E-5 RPP1 & pyrimidine depletion 23 3.63E-5
15 SGTC_2390 9097792 3.95 176.6 µM 2.13E-5 14 3.86E-5
16 SGTC_1532 4',5'-dihydroxyflavone 3.94 78.7 µM 2.30E-5 Golgi 20 4.15E-5
17 SGTC_2474 5321570 3.85 43.3 µM 3.36E-5 15 5.90E-5
18 SGTC_1583 clindamycin 3.82 39.6 µM 3.75E-5 mitochondrial processes 17 6.54E-5
19 SGTC_2159 5658611 3.79 73.8 µM 4.32E-5 14 7.47E-5
20 SGTC_2301 7769476 3.79 200.0 µM 4.35E-5 26 7.52E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.140 4.66E-16 YDR419W RAD30 hom DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
0.112 7.50E-11 YKR047W_d YKR047W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1
0.111 1.12E-10 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.085 9.37E-7 YJL150W_d YJL150W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.083 1.61E-6 YAL018C_p YAL018C_p hom Putative protein of unknown function
0.081 2.79E-6 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.080 3.17E-6 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.077 8.00E-6 YBL087C RPL23A hom Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication
0.076 1.12E-5 YDR126W SWF1 hom Palmitoyltransferase that acts on transmembrane proteins, including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
0.074 1.63E-5 YPR095C SYT1 hom Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain
0.073 2.57E-5 YLR131C ACE2 hom Transcription factor required for septum destruction after cytokinesis; phosphorylation by Cbk1p blocks nuclear exit during M/G1 transition, causing localization to daughter cell nuclei, and also increases Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; part of RAM network that regulates cellular polarity and morphogenesis; ACE2 has a paralog, SWI5, that arose from the whole genome duplication
0.072 2.70E-5 YER105C NUP157 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication
0.072 3.34E-5 YBL060W YEL1 hom Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip
0.072 3.41E-5 YBR273C UBX7 hom UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication
0.070 4.73E-5 YNR055C HOL1 hom Putative transporter in the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake