YKL184W / SPE1

Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL184W / SPE1

Click on Significant Values for Screen Details ID:SGTC_162|Compound:k030-0015|FD-Score:3.19|P-value:6.19E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.70|P-value:7.68E-5 ID:SGTC_281|Compound:1672-3440|FD-Score:3.58|P-value:1.41E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.11|P-value:7.36E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.53|P-value:1.74E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:-3.37|P-value:2.84E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.06|P-value:9.51E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:-3.31|P-value:3.57E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:-3.06|P-value:8.63E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.39|P-value:2.99E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.12|P-value:7.04E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.19|P-value:6.11E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.43|P-value:2.49E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.13|P-value:7.57E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-4.75|P-value:6.16E-7 ID:SGTC_2956|Compound:9076196|FD-Score:4.83|P-value:4.70E-7 ID:SGTC_2957|Compound:9076800|FD-Score:-3.05|P-value:9.07E-4 ID:SGTC_2961|Compound:9083290|FD-Score:4.77|P-value:6.54E-7 ID:SGTC_2962|Compound:9082311|FD-Score:3.09|P-value:8.66E-4 ID:SGTC_2987|Compound:9018338|FD-Score:3.08|P-value:9.12E-4 ID:SGTC_3035|Compound:9091303|FD-Score:3.07|P-value:9.44E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.31|P-value:3.50E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:3.19|P-value:6.19E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.70|P-value:7.68E-5 ID:SGTC_281|Compound:1672-3440|FD-Score:3.58|P-value:1.41E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.11|P-value:7.36E-4 ID:SGTC_446|Compound:go6976|FD-Score:3.53|P-value:1.74E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:-3.37|P-value:2.84E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.06|P-value:9.51E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:-3.31|P-value:3.57E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:-3.06|P-value:8.63E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:3.39|P-value:2.99E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:-3.12|P-value:7.04E-4 ID:SGTC_1756|Compound:st041558|FD-Score:3.19|P-value:6.11E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.43|P-value:2.49E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.13|P-value:7.57E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-4.75|P-value:6.16E-7 ID:SGTC_2956|Compound:9076196|FD-Score:4.83|P-value:4.70E-7 ID:SGTC_2957|Compound:9076800|FD-Score:-3.05|P-value:9.07E-4 ID:SGTC_2961|Compound:9083290|FD-Score:4.77|P-value:6.54E-7 ID:SGTC_2962|Compound:9082311|FD-Score:3.09|P-value:8.66E-4 ID:SGTC_2987|Compound:9018338|FD-Score:3.08|P-value:9.12E-4 ID:SGTC_3035|Compound:9091303|FD-Score:3.07|P-value:9.44E-4 ID:SGTC_3211|Compound:9128781|FD-Score:-3.31|P-value:3.50E-4

Top fitness defect scores for YKL184W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2956 9076196 4.83 2.5 µM 4.70E-7 7 6.80E-7
2 SGTC_2961 9083290 4.77 32.5 µM 6.54E-7 9 9.37E-7
3 SGTC_281 1672-3440 3.58 14.9 µM 1.41E-4 Golgi 8 1.71E-4
4 SGTC_446 go6976 3.53 132.0 µM 1.74E-4 20 2.09E-4
5 SGTC_2419 paclitaxel 3.43 166.5 µM 2.49E-4 73 2.97E-4
6 SGTC_1348 1493-0373 3.39 93.8 µM 2.99E-4 26 3.55E-4
7 SGTC_1756 st041558 3.19 35.5 µM 6.11E-4 27 7.10E-4
8 SGTC_162 k030-0015 3.19 37.8 µM 6.19E-4 iron homeostasis 41 7.20E-4
9 SGTC_2697 mebhydrolin 3.13 72.4 µM 7.57E-4 NEO1 113 8.75E-4
10 SGTC_2962 9082311 3.09 13.2 µM 8.66E-4 61 9.97E-4
11 SGTC_2987 9018338 3.08 71.4 µM 9.12E-4 RPP1 & pyrimidine depletion 65 0.00105
12 SGTC_3035 9091303 3.07 49.5 µM 9.44E-4 22 0.00108
13 SGTC_929 2805-0021 3.06 398.0 µM 9.51E-4 53 0.00109
14 SGTC_2930 9013230 3.03 14.9 µM 0.00107 heme biosynthesis & mitochondrial translocase 89 0.00123
15 SGTC_1758 st042942 2.98 75.2 µM 0.00129 57 0.00146
16 SGTC_972 1319-0219 2.93 195.0 µM 0.00150 79 0.00170
17 SGTC_1753 st043925 2.92 64.0 µM 0.00155 44 0.00175
18 SGTC_1905 st060207 2.88 84.5 µM 0.00175 46 0.00197
19 SGTC_1594 piperine 2.85 70.1 µM 0.00194 78 0.00218
20 SGTC_534 k292-0788 2.83 63.4 µM 0.00206 mitochondrial processes 79 0.00232

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.204 6.01E-33 YDR179C CSN9 hom Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
0.168 1.06E-22 YKL161C KDX1 hom Protein kinase implicated in the Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p
0.156 1.15E-19 YEL001C IRC22 hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
0.136 2.86E-15 YJR152W DAL5 hom Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
0.130 4.45E-14 YEL063C CAN1 hom Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
0.129 7.12E-14 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.127 1.86E-13 YDL168W SFA1 hom Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
0.113 5.33E-11 YDR215C_d YDR215C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein
0.113 6.65E-11 YLL062C MHT1 hom S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
0.112 7.05E-11 YJR061W_p YJR061W_p hom Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication
0.112 8.97E-11 YDR497C ITR1 hom Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress
0.108 4.17E-10 YKL164C PIR1 hom O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
0.107 6.33E-10 YJL122W ALB1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p
0.106 6.88E-10 YGL156W AMS1 hom Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway
0.105 1.11E-9 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly