YKL220C / FRE2

Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL220C / FRE2

Click on Significant Values for Screen Details ID:SGTC_144|Compound:4161-2749|FD-Score:-2.87|P-value:7.39E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:2.94|P-value:5.02E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.49|P-value:5.31E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:2.86|P-value:6.67E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:-2.95|P-value:5.22E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:2.87|P-value:6.64E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.41|P-value:7.75E-5 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.33|P-value:1.07E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-2.80|P-value:9.50E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:2.93|P-value:5.06E-4 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.86|P-value:7.80E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.07|P-value:3.34E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.40|P-value:4.91E-7 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.78|P-value:1.32E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:2.80|P-value:8.53E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:2.98|P-value:4.20E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-4.62|P-value:1.40E-7 ID:SGTC_1291|Compound:0993-0025|FD-Score:2.95|P-value:4.81E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.02|P-value:3.97E-4 ID:SGTC_1563|Compound:conessine|FD-Score:2.87|P-value:6.60E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.83|P-value:1.03E-5 ID:SGTC_2050|Compound:5263056|FD-Score:2.96|P-value:4.60E-4 ID:SGTC_2125|Compound:5246583|FD-Score:2.97|P-value:4.39E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-3.08|P-value:3.17E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-2.87|P-value:7.39E-4 ID:SGTC_2337|Compound:9009472|FD-Score:5.07|P-value:7.26E-9 ID:SGTC_2400|Compound:5203494|FD-Score:-3.89|P-value:7.56E-6 ID:SGTC_2602|Compound:kawain|FD-Score:2.79|P-value:8.91E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.11|P-value:2.43E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:2.81|P-value:8.27E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.38|P-value:8.88E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.89|P-value:7.83E-6 ID:SGTC_2952|Compound:9073731|FD-Score:-2.90|P-value:6.58E-4 ID:SGTC_3021|Compound:9087658|FD-Score:3.12|P-value:2.40E-4 ID:SGTC_3041|Compound:9091929|FD-Score:3.02|P-value:3.65E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-3.38|P-value:8.62E-5 ID:SGTC_3313|Compound:9133968|FD-Score:3.30|P-value:1.11E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-2.87|P-value:7.39E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:2.94|P-value:5.02E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.49|P-value:5.31E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:2.86|P-value:6.67E-4 ID:SGTC_330|Compound:1488-0319|FD-Score:-2.95|P-value:5.22E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:2.87|P-value:6.64E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.41|P-value:7.75E-5 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.33|P-value:1.07E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:-2.80|P-value:9.50E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:2.93|P-value:5.06E-4 ID:SGTC_1009|Compound:4106-0057|FD-Score:3.86|P-value:7.80E-6 ID:SGTC_1072|Compound:oxiconazole|FD-Score:-3.07|P-value:3.34E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.40|P-value:4.91E-7 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.78|P-value:1.32E-5 ID:SGTC_1134|Compound:3477-0009|FD-Score:2.80|P-value:8.53E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:2.98|P-value:4.20E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-4.62|P-value:1.40E-7 ID:SGTC_1291|Compound:0993-0025|FD-Score:2.95|P-value:4.81E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.02|P-value:3.97E-4 ID:SGTC_1563|Compound:conessine|FD-Score:2.87|P-value:6.60E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.83|P-value:1.03E-5 ID:SGTC_2050|Compound:5263056|FD-Score:2.96|P-value:4.60E-4 ID:SGTC_2125|Compound:5246583|FD-Score:2.97|P-value:4.39E-4 ID:SGTC_2128|Compound:5255449|FD-Score:-3.08|P-value:3.17E-4 ID:SGTC_2332|Compound:7982454|FD-Score:-2.87|P-value:7.39E-4 ID:SGTC_2337|Compound:9009472|FD-Score:5.07|P-value:7.26E-9 ID:SGTC_2400|Compound:5203494|FD-Score:-3.89|P-value:7.56E-6 ID:SGTC_2602|Compound:kawain|FD-Score:2.79|P-value:8.91E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.11|P-value:2.43E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:2.81|P-value:8.27E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.38|P-value:8.88E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.89|P-value:7.83E-6 ID:SGTC_2952|Compound:9073731|FD-Score:-2.90|P-value:6.58E-4 ID:SGTC_3021|Compound:9087658|FD-Score:3.12|P-value:2.40E-4 ID:SGTC_3041|Compound:9091929|FD-Score:3.02|P-value:3.65E-4 ID:SGTC_3166|Compound:9102297|FD-Score:-3.38|P-value:8.62E-5 ID:SGTC_3313|Compound:9133968|FD-Score:3.30|P-value:1.11E-4

Top fitness defect scores for YKL220C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2337 9009472 5.07 200.0 µM 7.26E-9 12 2.00E-7
2 SGTC_1009 4106-0057 3.86 9.9 µM 7.80E-6 13 5.64E-5
3 SGTC_3313 9133968 3.30 57.0 µM 1.11E-4 19 4.85E-4
4 SGTC_3021 9087658 3.12 49.5 µM 2.40E-4 21 9.10E-4
5 SGTC_2621 lithochol-11-enic acid 3.11 100.0 µM 2.43E-4 70 9.21E-4
6 SGTC_3041 9091929 3.02 49.5 µM 3.65E-4 redox potentiating 60 0.00128
7 SGTC_1203 3992-3522 2.98 103.0 µM 4.20E-4 90 0.00144
8 SGTC_2125 5246583 2.97 200.0 µM 4.39E-4 tubulin folding & SWR complex 100 0.00149
9 SGTC_2050 5263056 2.96 171.0 µM 4.60E-4 fatty acid desaturase (OLE1) 101 0.00155
10 SGTC_1291 0993-0025 2.95 5.8 µM 4.81E-4 39 0.00160
11 SGTC_152 4469-0003 2.94 551.2 µM 5.02E-4 58 0.00166
12 SGTC_1008 4106-0037 2.93 6.0 µM 5.06E-4 PDR1 51 0.00167
13 SGTC_1563 conessine 2.87 56.1 µM 6.60E-4 66 0.00207
14 SGTC_366 0181-0206 2.87 4.3 µM 6.64E-4 Golgi 99 0.00208
15 SGTC_189 3970-0742 2.86 2.1 µM 6.67E-4 32 0.00209
16 SGTC_2687 chenodiol 2.81 100.0 µM 8.27E-4 83 0.00249
17 SGTC_1134 3477-0009 2.80 42.5 µM 8.53E-4 115 0.00256
18 SGTC_2602 kawain 2.79 100.0 µM 8.91E-4 98 0.00265
19 SGTC_2686 alizarin 2.71 95.4 µM 0.00121 RPP1 & pyrimidine depletion 96 0.00340
20 SGTC_1059 3448-6867 2.65 171.0 µM 0.00150 77 0.00405

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.342 1.60E-92 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.326 8.83E-84 YAL061W_p BDH2_p hom Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
0.288 4.34E-65 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.275 4.14E-59 YLR353W BUD8 hom Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
0.272 5.98E-58 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication
0.268 4.30E-56 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.249 1.33E-48 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.247 4.94E-48 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.231 4.88E-42 YLR016C PML1 hom Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.223 6.15E-39 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.217 5.14E-37 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.215 2.98E-36 YMR147W_p YMR147W_p hom Putative protein of unknown function
0.210 6.56E-35 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.209 1.33E-34 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.208 3.72E-34 YDR122W KIN1 hom Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p