HIPHOP chemogenomics database

YLR020C / YEH2

Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR020C / YEH2

Click on Significant Values for Screen Details

Top fitness defect scores for YLR020C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1760	st045453	19.30	10.4 µM	1.68E-94	NEO1-PIK1	8	1.88E-83
2	SGTC_2651	1327-0036	13.50	5.0 µM	1.78E-47		12	4.52E-42
3	SGTC_1754	st042065	12.90	16.1 µM	2.60E-43	NEO1-PIK1	13	2.10E-38
4	SGTC_1801	st050089	11.70	28.4 µM	3.71E-36	NEO1-PIK1	9	4.18E-32
5	SGTC_1803	st050068	11.50	52.4 µM	5.57E-35	NEO1-PIK1	18	4.53E-31
6	SGTC_2582	agaric acid	11.50	100.0 µM	1.48E-34	redox potentiating	45	1.07E-30
7	SGTC_221	trifluoperazine	11.40	10.3 µM	2.74E-34	NEO1-PIK1	43	1.84E-30
8	SGTC_2896	9052656	11.20	71.4 µM	4.54E-33	endomembrane recycling	7	2.19E-29
9	SGTC_1818	st052136	7.28	56.3 µM	4.76E-15	NEO1-PIK1	6	1.68E-13
10	SGTC_1800	st050079	6.77	29.1 µM	3.05E-13	NEO1-PIK1	28	6.62E-12
11	SGTC_2249	haloperidol	6.76	116.4 µM	3.28E-13	ergosterol depletion effects on membrane	34	7.05E-12
12	SGTC_1597	7-benzyloxygramine	6.54	47.5 µM	1.76E-12	calcium & mitochondrial duress	44	3.10E-11
13	SGTC_1750	st081859	6.52	2.4 µM	2.06E-12	endomembrane recycling	96	3.57E-11
14	SGTC_312	4092-0653	5.93	6.8 µM	1.38E-10	ergosterol depletion effects on membrane	40	1.47E-9
15	SGTC_2021	4019055	5.71	173.0 µM	6.19E-10	endomembrane recycling	45	5.53E-9
16	SGTC_2250	alverine citrate	5.63	130.0 µM	1.07E-9		67	8.97E-9
17	SGTC_1719	st032227	5.62	49.2 µM	1.18E-9		6	9.80E-9
18	SGTC_649	1123-0311	5.44	367.0 µM	3.57E-9	endomembrane recycling	32	2.60E-8
19	SGTC_207	4185-0010	5.39	4.5 µM	5.16E-9	endomembrane recycling	51	3.61E-8
20	SGTC_377	3970-0964	5.13	33.1 µM	2.53E-8		26	1.47E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.492	7.18E-204	YML097C	VPS9	hom	A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.471	2.02E-185	YHR108W	GGA2	hom	Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.466	6.29E-180	YNL267W	PIK1	het	Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.457	3.57E-173	YOR115C	TRS33	hom	One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.454	2.33E-170	YBR246W	RRT2	hom	Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.452	1.21E-168	YML037C_p	YML037C_p	hom	Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.450	3.33E-167	YNL183C	NPR1	hom	Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.439	1.81E-158	YPR091C	NVJ2	hom	Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.439	2.39E-158	YKL191W	DPH2	hom	Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.438	3.13E-157	YOR357C	SNX3	hom	Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.434	1.62E-154	YDR492W	IZH1	hom	Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.424	1.45E-146	YJL154C	VPS35	hom	Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.416	1.02E-140	YHL019C	APM2	hom	Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.395	1.79E-125	YPR092W_d	YPR092W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.383	2.00E-117	YDR084C	TVP23	hom	Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern