YLR089C / ALT1

Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthetic and catabolic processes; expression is induced in the presence of alanine; repression is mediated by Nrg1p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; paralogous gene ALT2 is catalytically inactive

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR089C / ALT1

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:-2.97|P-value:7.53E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:3.02|P-value:7.85E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:3.10|P-value:5.70E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.18|P-value:5.72E-6 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.10|P-value:4.72E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.94|P-value:1.79E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.12|P-value:7.37E-6 ID:SGTC_441|Compound:propafenone|FD-Score:-3.79|P-value:2.70E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:-3.10|P-value:4.58E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.26|P-value:2.55E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.55|P-value:9.85E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.30|P-value:2.68E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:4.69|P-value:4.17E-7 ID:SGTC_1278|Compound:0874-0585|FD-Score:4.02|P-value:1.24E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:4.19|P-value:5.32E-6 ID:SGTC_1476|Compound:4112-3294|FD-Score:4.02|P-value:1.22E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.11|P-value:5.66E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.89|P-value:2.22E-5 ID:SGTC_1655|Compound:st012546|FD-Score:3.02|P-value:7.70E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.77|P-value:3.82E-5 ID:SGTC_1821|Compound:st053465|FD-Score:3.81|P-value:3.16E-5 ID:SGTC_1963|Compound:st076565|FD-Score:3.45|P-value:1.45E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.98|P-value:1.47E-5 ID:SGTC_2122|Compound:5258151|FD-Score:4.13|P-value:7.13E-6 ID:SGTC_2264|Compound:7948757|FD-Score:3.27|P-value:3.07E-4 ID:SGTC_2281|Compound:7943571|FD-Score:-3.07|P-value:5.14E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-4.86|P-value:1.16E-7 ID:SGTC_2308|Compound:7584454|FD-Score:2.96|P-value:9.58E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-3.15|P-value:3.77E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-3.90|P-value:1.64E-5 ID:SGTC_2344|Compound:7990960|FD-Score:5.51|P-value:3.41E-9 ID:SGTC_2396|Compound:5342950|FD-Score:3.68|P-value:5.54E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.99|P-value:1.38E-5 ID:SGTC_2467|Compound:5474540|FD-Score:3.18|P-value:4.23E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-3.41|P-value:1.37E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.02|P-value:6.37E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-4.38|P-value:1.53E-6 ID:SGTC_2581|Compound:avocatin a|FD-Score:5.24|P-value:1.77E-8 ID:SGTC_2721|Compound:ifenprodil|FD-Score:-2.94|P-value:8.49E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.27|P-value:2.43E-4 ID:SGTC_2833|Compound:9001315|FD-Score:4.07|P-value:9.46E-6 ID:SGTC_2834|Compound:9001755|FD-Score:3.19|P-value:4.08E-4 ID:SGTC_2994|Compound:9053520|FD-Score:2.96|P-value:9.71E-4 ID:SGTC_2999|Compound:9069765|FD-Score:3.16|P-value:4.63E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.22|P-value:4.63E-6 ID:SGTC_3216|Compound:9129716|FD-Score:3.99|P-value:1.42E-5 ID:SGTC_69|Compound:0096-0274|FD-Score:-2.97|P-value:7.53E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:3.02|P-value:7.85E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:3.10|P-value:5.70E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:4.18|P-value:5.72E-6 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.10|P-value:4.72E-4 ID:SGTC_419|Compound:nsc-638432|FD-Score:3.94|P-value:1.79E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:4.12|P-value:7.37E-6 ID:SGTC_441|Compound:propafenone|FD-Score:-3.79|P-value:2.70E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:-3.10|P-value:4.58E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.26|P-value:2.55E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.55|P-value:9.85E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.30|P-value:2.68E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:4.69|P-value:4.17E-7 ID:SGTC_1278|Compound:0874-0585|FD-Score:4.02|P-value:1.24E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:4.19|P-value:5.32E-6 ID:SGTC_1476|Compound:4112-3294|FD-Score:4.02|P-value:1.22E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.11|P-value:5.66E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:3.89|P-value:2.22E-5 ID:SGTC_1655|Compound:st012546|FD-Score:3.02|P-value:7.70E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.77|P-value:3.82E-5 ID:SGTC_1821|Compound:st053465|FD-Score:3.81|P-value:3.16E-5 ID:SGTC_1963|Compound:st076565|FD-Score:3.45|P-value:1.45E-4 ID:SGTC_2114|Compound:5491910|FD-Score:3.98|P-value:1.47E-5 ID:SGTC_2122|Compound:5258151|FD-Score:4.13|P-value:7.13E-6 ID:SGTC_2264|Compound:7948757|FD-Score:3.27|P-value:3.07E-4 ID:SGTC_2281|Compound:7943571|FD-Score:-3.07|P-value:5.14E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-4.86|P-value:1.16E-7 ID:SGTC_2308|Compound:7584454|FD-Score:2.96|P-value:9.58E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-3.15|P-value:3.77E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-3.90|P-value:1.64E-5 ID:SGTC_2344|Compound:7990960|FD-Score:5.51|P-value:3.41E-9 ID:SGTC_2396|Compound:5342950|FD-Score:3.68|P-value:5.54E-5 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.99|P-value:1.38E-5 ID:SGTC_2467|Compound:5474540|FD-Score:3.18|P-value:4.23E-4 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-3.41|P-value:1.37E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.02|P-value:6.37E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-4.38|P-value:1.53E-6 ID:SGTC_2581|Compound:avocatin a|FD-Score:5.24|P-value:1.77E-8 ID:SGTC_2721|Compound:ifenprodil|FD-Score:-2.94|P-value:8.49E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.27|P-value:2.43E-4 ID:SGTC_2833|Compound:9001315|FD-Score:4.07|P-value:9.46E-6 ID:SGTC_2834|Compound:9001755|FD-Score:3.19|P-value:4.08E-4 ID:SGTC_2994|Compound:9053520|FD-Score:2.96|P-value:9.71E-4 ID:SGTC_2999|Compound:9069765|FD-Score:3.16|P-value:4.63E-4 ID:SGTC_3068|Compound:9116768|FD-Score:4.22|P-value:4.63E-6 ID:SGTC_3216|Compound:9129716|FD-Score:3.99|P-value:1.42E-5

Top fitness defect scores for YLR089C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2344 7990960 5.51 77.0 µM 3.41E-9 9 1.83E-8
2 SGTC_2581 avocatin a 5.24 64.0 µM 1.77E-8 sphingolipid biosynthesis & PDR1 31 8.05E-8
3 SGTC_1150 3909-7983 4.69 10.9 µM 4.17E-7 11 1.39E-6
4 SGTC_3068 9116768 4.22 49.5 µM 4.63E-6 14 1.22E-5
5 SGTC_1280 0880-0253 4.19 10.1 µM 5.32E-6 12 1.38E-5
6 SGTC_184 1494-0393 4.18 24.7 µM 5.72E-6 52 1.48E-5
7 SGTC_2122 5258151 4.13 111.7 µM 7.13E-6 20 1.80E-5
8 SGTC_423 5-fluorouracil 4.12 457.0 µM 7.37E-6 exosome 19 1.86E-5
9 SGTC_2833 9001315 4.07 45.5 µM 9.46E-6 amide catabolism 50 2.33E-5
10 SGTC_1476 4112-3294 4.02 3.3 µM 1.22E-5 60S ribosome export 13 2.93E-5
11 SGTC_1278 0874-0585 4.02 172.0 µM 1.24E-5 14 2.97E-5
12 SGTC_2408 sulbentine 3.99 18.4 µM 1.38E-5 49 3.28E-5
13 SGTC_3216 9129716 3.99 49.5 µM 1.42E-5 RPP1 & pyrimidine depletion 23 3.36E-5
14 SGTC_2114 5491910 3.98 81.2 µM 1.47E-5 12 3.46E-5
15 SGTC_419 nsc-638432 3.94 893.0 µM 1.79E-5 DNA damage response 12 4.14E-5
16 SGTC_1541 2',3',6-trimethoxyflavone 3.89 64.0 µM 2.22E-5 14 5.03E-5
17 SGTC_1821 st053465 3.81 65.6 µM 3.16E-5 8 6.94E-5
18 SGTC_1715 st032160 3.77 10.2 µM 3.82E-5 47 8.22E-5
19 SGTC_2396 5342950 3.68 42.8 µM 5.54E-5 RSC complex & mRNA processing 55 1.15E-4
20 SGTC_976 1348-1067 3.55 47.6 µM 9.85E-5 60S ribosome export 46 1.94E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.119 4.16E-12 YGL164C YRB30 hom RanGTP-binding protein, inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes
0.117 1.02E-11 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.110 1.45E-10 YJR049C UTR1 hom ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
0.109 2.60E-10 YLR098C CHA4 hom DNA binding transcriptional activator, mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
0.102 3.06E-9 YPL091W GLR1 hom Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
0.093 6.87E-8 YJR043C POL32 hom Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p
0.090 1.73E-7 YNL157W IGO1 hom Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p
0.088 3.32E-7 YDL069C CBS1 hom Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
0.085 8.26E-7 YBR059C AKL1 hom Ser-Thr protein kinase, member (with Ark1p and Prk1p) of the Ark kinase family; involved in endocytosis and actin cytoskeleton organization
0.084 1.20E-6 YMR306C-A_d YMR306C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.082 2.08E-6 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.082 2.14E-6 YLR010C TEN1 het Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p
0.081 2.54E-6 YGR017W_p YGR017W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
0.081 2.98E-6 YMR086C-A_d YMR086C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.079 4.70E-6 YGR059W SPR3 hom Sporulation-specific homolog of the yeast CDC3/10/11/12 family of bud neck microfilament genes; septin protein involved in sporulation; regulated by ABFI