YLR133W / CKI1

Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR133W / CKI1

Click on Significant Values for Screen Details ID:SGTC_59|Compound:0330-0160|FD-Score:5.09|P-value:5.94E-6 ID:SGTC_77|Compound:0828-0286|FD-Score:3.95|P-value:3.56E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:4.73|P-value:2.40E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:6.75|P-value:2.94E-9 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.64|P-value:4.28E-14 ID:SGTC_264|Compound:promethazine|FD-Score:10.50|P-value:4.41E-20 ID:SGTC_310|Compound:1273-0058|FD-Score:-6.23|P-value:2.54E-8 ID:SGTC_365|Compound:0121-0052|FD-Score:-4.73|P-value:1.66E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:-6.00|P-value:7.57E-8 ID:SGTC_377|Compound:3970-0964|FD-Score:-4.09|P-value:1.63E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:5.35|P-value:2.12E-6 ID:SGTC_425|Compound:3825-7017|FD-Score:-5.91|P-value:1.14E-7 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.70|P-value:5.70E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.03|P-value:7.75E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:7.88|P-value:5.14E-12 ID:SGTC_862|Compound:0452-0056|FD-Score:-4.99|P-value:6.05E-6 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.77|P-value:6.25E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-3.68|P-value:6.06E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.89|P-value:4.33E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:-4.96|P-value:6.83E-6 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.86|P-value:3.45E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:-3.65|P-value:6.60E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:-4.04|P-value:1.94E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.31|P-value:1.07E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:4.02|P-value:2.82E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.62|P-value:9.71E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.93|P-value:2.77E-4 ID:SGTC_1701|Compound:st028638|FD-Score:3.91|P-value:4.02E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.77|P-value:6.17E-4 ID:SGTC_1754|Compound:st042065|FD-Score:6.28|P-value:3.20E-8 ID:SGTC_1818|Compound:st052136|FD-Score:4.08|P-value:2.31E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:8.12|P-value:1.23E-12 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:5.13|P-value:5.10E-6 ID:SGTC_1933|Compound:st074711|FD-Score:3.62|P-value:9.66E-4 ID:SGTC_1989|Compound:st071868|FD-Score:-3.55|P-value:8.84E-4 ID:SGTC_2062|Compound:5245942|FD-Score:3.99|P-value:3.09E-4 ID:SGTC_2064|Compound:5220758|FD-Score:4.30|P-value:1.10E-4 ID:SGTC_2072|Compound:5231554|FD-Score:4.54|P-value:4.87E-5 ID:SGTC_2120|Compound:5538279|FD-Score:4.89|P-value:1.33E-5 ID:SGTC_2292|Compound:7671323|FD-Score:-3.57|P-value:8.48E-4 ID:SGTC_2298|Compound:7976326|FD-Score:-3.59|P-value:7.96E-4 ID:SGTC_2300|Compound:7976697|FD-Score:4.65|P-value:3.25E-5 ID:SGTC_2423|Compound:st077119|FD-Score:4.58|P-value:4.20E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.93|P-value:1.18E-9 ID:SGTC_2791|Compound:5663188|FD-Score:-4.11|P-value:1.50E-4 ID:SGTC_2853|Compound:9015042|FD-Score:3.65|P-value:8.93E-4 ID:SGTC_2857|Compound:9024691|FD-Score:4.14|P-value:1.90E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-3.56|P-value:8.55E-4 ID:SGTC_2961|Compound:9083290|FD-Score:4.27|P-value:1.24E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.72|P-value:7.18E-4 ID:SGTC_3040|Compound:9091894|FD-Score:4.38|P-value:8.46E-5 ID:SGTC_3305|Compound:9127027|FD-Score:-4.00|P-value:2.19E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:5.09|P-value:5.94E-6 ID:SGTC_77|Compound:0828-0286|FD-Score:3.95|P-value:3.56E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:4.73|P-value:2.40E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:6.75|P-value:2.94E-9 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.64|P-value:4.28E-14 ID:SGTC_264|Compound:promethazine|FD-Score:10.50|P-value:4.41E-20 ID:SGTC_310|Compound:1273-0058|FD-Score:-6.23|P-value:2.54E-8 ID:SGTC_365|Compound:0121-0052|FD-Score:-4.73|P-value:1.66E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:-6.00|P-value:7.57E-8 ID:SGTC_377|Compound:3970-0964|FD-Score:-4.09|P-value:1.63E-4 ID:SGTC_400|Compound:oxethazaine|FD-Score:5.35|P-value:2.12E-6 ID:SGTC_425|Compound:3825-7017|FD-Score:-5.91|P-value:1.14E-7 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.70|P-value:5.70E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:5.03|P-value:7.75E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:7.88|P-value:5.14E-12 ID:SGTC_862|Compound:0452-0056|FD-Score:-4.99|P-value:6.05E-6 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.77|P-value:6.25E-4 ID:SGTC_1048|Compound:3161-0300|FD-Score:-3.68|P-value:6.06E-4 ID:SGTC_1052|Compound:3253-3998|FD-Score:3.89|P-value:4.33E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:-4.96|P-value:6.83E-6 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.86|P-value:3.45E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:-3.65|P-value:6.60E-4 ID:SGTC_1351|Compound:1496-0003|FD-Score:-4.04|P-value:1.94E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.31|P-value:1.07E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:4.02|P-value:2.82E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.62|P-value:9.71E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.93|P-value:2.77E-4 ID:SGTC_1701|Compound:st028638|FD-Score:3.91|P-value:4.02E-4 ID:SGTC_1706|Compound:st031321|FD-Score:3.77|P-value:6.17E-4 ID:SGTC_1754|Compound:st042065|FD-Score:6.28|P-value:3.20E-8 ID:SGTC_1818|Compound:st052136|FD-Score:4.08|P-value:2.31E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:8.12|P-value:1.23E-12 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:5.13|P-value:5.10E-6 ID:SGTC_1933|Compound:st074711|FD-Score:3.62|P-value:9.66E-4 ID:SGTC_1989|Compound:st071868|FD-Score:-3.55|P-value:8.84E-4 ID:SGTC_2062|Compound:5245942|FD-Score:3.99|P-value:3.09E-4 ID:SGTC_2064|Compound:5220758|FD-Score:4.30|P-value:1.10E-4 ID:SGTC_2072|Compound:5231554|FD-Score:4.54|P-value:4.87E-5 ID:SGTC_2120|Compound:5538279|FD-Score:4.89|P-value:1.33E-5 ID:SGTC_2292|Compound:7671323|FD-Score:-3.57|P-value:8.48E-4 ID:SGTC_2298|Compound:7976326|FD-Score:-3.59|P-value:7.96E-4 ID:SGTC_2300|Compound:7976697|FD-Score:4.65|P-value:3.25E-5 ID:SGTC_2423|Compound:st077119|FD-Score:4.58|P-value:4.20E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.93|P-value:1.18E-9 ID:SGTC_2791|Compound:5663188|FD-Score:-4.11|P-value:1.50E-4 ID:SGTC_2853|Compound:9015042|FD-Score:3.65|P-value:8.93E-4 ID:SGTC_2857|Compound:9024691|FD-Score:4.14|P-value:1.90E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-3.56|P-value:8.55E-4 ID:SGTC_2961|Compound:9083290|FD-Score:4.27|P-value:1.24E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.72|P-value:7.18E-4 ID:SGTC_3040|Compound:9091894|FD-Score:4.38|P-value:8.46E-5 ID:SGTC_3305|Compound:9127027|FD-Score:-4.00|P-value:2.19E-4

Top fitness defect scores for YLR133W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_264 promethazine 10.50 12.5 mM 4.41E-20 DNA intercalators 9 3.07E-26
2 SGTC_221 trifluoperazine 8.64 10.3 µM 4.28E-14 NEO1-PIK1 77 2.76E-18
3 SGTC_1926 methyl fluorone black 8.12 53.6 µM 1.23E-12 DNA intercalators 7 2.39E-16
4 SGTC_568 1193-0097 7.88 7.5 µM 5.14E-12 ERAD & cell cycle 37 1.60E-15
5 SGTC_2688 kanamycin b 6.93 96.7 µM 1.18E-9 calcium & mitochondrial duress 110 2.17E-12
6 SGTC_145 3737-0113 6.75 38.4 µM 2.94E-9 DNA intercalators 17 7.29E-12
7 SGTC_1754 st042065 6.28 16.1 µM 3.20E-8 NEO1-PIK1 90 1.73E-10
8 SGTC_400 oxethazaine 5.35 37.5 µM 2.12E-6 calcium & mitochondrial duress 8 4.50E-8
9 SGTC_1928 4'-methoxychalcone 5.13 40.3 µM 5.10E-6 2 1.44E-7
10 SGTC_59 0330-0160 5.09 1.3 µM 5.94E-6 57 1.76E-7
11 SGTC_513 4245-1575 5.03 41.7 µM 7.75E-6 unfolded protein response 72 2.50E-7
12 SGTC_2120 5538279 4.89 152.0 µM 1.33E-5 5 5.11E-7
13 SGTC_144 4161-2749 4.73 112.4 µM 2.40E-5 DNA intercalators 13 1.12E-6
14 SGTC_2300 7976697 4.65 71.6 µM 3.25E-5 ERG2 17 1.67E-6
15 SGTC_2423 st077119 4.58 87.2 µM 4.20E-5 7 2.34E-6
16 SGTC_2072 5231554 4.54 129.0 µM 4.87E-5 8 2.85E-6
17 SGTC_3040 9091894 4.38 49.5 µM 8.46E-5 DNA intercalators 15 5.92E-6
18 SGTC_1468 k284-1086 4.31 134.0 µM 1.07E-4 DNA intercalators 25 8.06E-6
19 SGTC_2064 5220758 4.30 200.0 µM 1.10E-4 25 8.38E-6
20 SGTC_2961 9083290 4.27 32.5 µM 1.24E-4 13 9.80E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.303 3.09E-72 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.303 4.67E-72 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.300 1.00E-70 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.294 9.48E-68 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.287 1.13E-64 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.267 9.24E-56 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.266 2.38E-55 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.254 2.20E-50 YEL032W MCM3 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.248 2.96E-48 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.211 3.22E-35 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.206 1.31E-33 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.206 1.91E-33 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.201 9.78E-32 YGL041C_d YGL041C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.200 9.39E-32 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.198 7.19E-31 YGL077C HNM1 hom Choline/ethanolamine transporter; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol