YLR137W / RKM5

Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR137W / RKM5

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.02|P-value:4.39E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:2.86|P-value:7.99E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:2.83|P-value:9.11E-4 ID:SGTC_307|Compound:1501-1223|FD-Score:4.34|P-value:8.51E-7 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.95|P-value:6.74E-6 ID:SGTC_521|Compound:r062-0005|FD-Score:3.72|P-value:2.11E-5 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.01|P-value:4.66E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.68|P-value:1.28E-7 ID:SGTC_736|Compound:0958-0254|FD-Score:3.25|P-value:1.67E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:3.24|P-value:1.74E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:4.37|P-value:7.33E-7 ID:SGTC_753|Compound:1322-0200|FD-Score:4.14|P-value:2.54E-6 ID:SGTC_767|Compound:0929-0023|FD-Score:-3.28|P-value:1.50E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.34|P-value:1.14E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:4.06|P-value:3.87E-6 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:2.86|P-value:8.02E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-2.82|P-value:9.49E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.06|P-value:3.78E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:-3.98|P-value:5.92E-6 ID:SGTC_1031|Compound:k035-0009|FD-Score:-2.91|P-value:6.63E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-2.91|P-value:6.78E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:-2.99|P-value:5.04E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.34|P-value:1.18E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.75|P-value:1.78E-5 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.60|P-value:3.68E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.47|P-value:6.59E-5 ID:SGTC_1363|Compound:1636-0063|FD-Score:-2.87|P-value:7.89E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:4.16|P-value:2.22E-6 ID:SGTC_1499|Compound:4480-0877|FD-Score:3.35|P-value:1.12E-4 ID:SGTC_1674|Compound:st016616|FD-Score:-3.68|P-value:2.56E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.50|P-value:5.64E-5 ID:SGTC_1852|Compound:st056254|FD-Score:3.15|P-value:2.63E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.89|P-value:7.27E-4 ID:SGTC_2123|Compound:5259487|FD-Score:2.89|P-value:7.27E-4 ID:SGTC_2272|Compound:7805186|FD-Score:3.09|P-value:3.30E-4 ID:SGTC_2343|Compound:7990716|FD-Score:3.26|P-value:1.65E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.10|P-value:3.22E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.24|P-value:1.81E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.29|P-value:1.43E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.05|P-value:3.83E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.54|P-value:4.73E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.76|P-value:1.70E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-3.10|P-value:3.20E-4 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.01|P-value:4.47E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-3.02|P-value:4.42E-4 ID:SGTC_3117|Compound:9124945|FD-Score:-2.84|P-value:8.76E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-3.00|P-value:4.68E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.15|P-value:2.54E-4 ID:SGTC_3250|Compound:9135583|FD-Score:3.07|P-value:3.57E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.07|P-value:3.50E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.02|P-value:4.39E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:2.86|P-value:7.99E-4 ID:SGTC_280|Compound:3448-3607|FD-Score:2.83|P-value:9.11E-4 ID:SGTC_307|Compound:1501-1223|FD-Score:4.34|P-value:8.51E-7 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.95|P-value:6.74E-6 ID:SGTC_521|Compound:r062-0005|FD-Score:3.72|P-value:2.11E-5 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.01|P-value:4.66E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.68|P-value:1.28E-7 ID:SGTC_736|Compound:0958-0254|FD-Score:3.25|P-value:1.67E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:3.24|P-value:1.74E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:4.37|P-value:7.33E-7 ID:SGTC_753|Compound:1322-0200|FD-Score:4.14|P-value:2.54E-6 ID:SGTC_767|Compound:0929-0023|FD-Score:-3.28|P-value:1.50E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:3.34|P-value:1.14E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:4.06|P-value:3.87E-6 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:2.86|P-value:8.02E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:-2.82|P-value:9.49E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.06|P-value:3.78E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:-3.98|P-value:5.92E-6 ID:SGTC_1031|Compound:k035-0009|FD-Score:-2.91|P-value:6.63E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:-2.91|P-value:6.78E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:-2.99|P-value:5.04E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.34|P-value:1.18E-4 ID:SGTC_1217|Compound:1183-1422|FD-Score:3.75|P-value:1.78E-5 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.60|P-value:3.68E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.47|P-value:6.59E-5 ID:SGTC_1363|Compound:1636-0063|FD-Score:-2.87|P-value:7.89E-4 ID:SGTC_1467|Compound:k284-1082|FD-Score:4.16|P-value:2.22E-6 ID:SGTC_1499|Compound:4480-0877|FD-Score:3.35|P-value:1.12E-4 ID:SGTC_1674|Compound:st016616|FD-Score:-3.68|P-value:2.56E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.50|P-value:5.64E-5 ID:SGTC_1852|Compound:st056254|FD-Score:3.15|P-value:2.63E-4 ID:SGTC_2103|Compound:5378138|FD-Score:2.89|P-value:7.27E-4 ID:SGTC_2123|Compound:5259487|FD-Score:2.89|P-value:7.27E-4 ID:SGTC_2272|Compound:7805186|FD-Score:3.09|P-value:3.30E-4 ID:SGTC_2343|Compound:7990716|FD-Score:3.26|P-value:1.65E-4 ID:SGTC_2384|Compound:9036245|FD-Score:3.10|P-value:3.22E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:3.24|P-value:1.81E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.29|P-value:1.43E-4 ID:SGTC_2537|Compound:larixol|FD-Score:3.05|P-value:3.83E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.54|P-value:4.73E-5 ID:SGTC_2773|Compound:thioridazine|FD-Score:-3.76|P-value:1.70E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-3.10|P-value:3.20E-4 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.01|P-value:4.47E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-3.02|P-value:4.42E-4 ID:SGTC_3117|Compound:9124945|FD-Score:-2.84|P-value:8.76E-4 ID:SGTC_3216|Compound:9129716|FD-Score:-3.00|P-value:4.68E-4 ID:SGTC_3217|Compound:9129673|FD-Score:3.15|P-value:2.54E-4 ID:SGTC_3250|Compound:9135583|FD-Score:3.07|P-value:3.57E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.07|P-value:3.50E-4

Top fitness defect scores for YLR137W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_669 0108-0021 4.68 26.2 µM 1.28E-7 fatty acid desaturase (OLE1) 50 1.46E-6
2 SGTC_749 1000-0129 4.37 128.0 µM 7.33E-7 amide catabolism 11 6.24E-6
3 SGTC_307 1501-1223 4.34 22.3 µM 8.51E-7 7 7.06E-6
4 SGTC_1467 k284-1082 4.16 293.0 µM 2.22E-6 14 1.56E-5
5 SGTC_753 1322-0200 4.14 289.0 µM 2.54E-6 13 1.75E-5
6 SGTC_909 3448-9333 4.06 149.0 µM 3.87E-6 16 2.48E-5
7 SGTC_1217 1183-1422 3.75 255.0 µM 1.78E-5 17 8.80E-5
8 SGTC_521 r062-0005 3.72 13.8 µM 2.11E-5 17 1.01E-4
9 SGTC_1307 1187-1599 3.60 12.2 µM 3.68E-5 PDR1 17 1.61E-4
10 SGTC_2714 st079313 3.54 56.9 µM 4.73E-5 13 1.99E-4
11 SGTC_1818 st052136 3.50 56.3 µM 5.64E-5 NEO1-PIK1 74 2.30E-4
12 SGTC_1313 1220-0047 3.47 237.0 µM 6.59E-5 57 2.62E-4
13 SGTC_1499 4480-0877 3.35 67.3 µM 1.12E-4 17 4.07E-4
14 SGTC_907 3448-6986 3.34 75.5 µM 1.14E-4 46 4.14E-4
15 SGTC_2343 7990716 3.26 14.8 µM 1.65E-4 40 5.63E-4
16 SGTC_736 0958-0254 3.25 92.6 µM 1.67E-4 Golgi 57 5.67E-4
17 SGTC_748 0136-0228 3.24 77.9 µM 1.74E-4 redox potentiating 110 5.87E-4
18 SGTC_2514 salinomycin 3.24 42.7 µM 1.81E-4 90 6.07E-4
19 SGTC_3217 9129673 3.15 49.5 µM 2.54E-4 80 8.05E-4
20 SGTC_1852 st056254 3.15 31.4 µM 2.63E-4 37 8.29E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.161 5.47E-21 YDL133C-A RPL41B hom Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication
0.118 8.35E-12 YDL180W_p YDL180W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.115 2.56E-11 YFL056C AAD6 hom Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin
0.105 1.18E-9 YBR186W PCH2 hom Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation
0.103 1.94E-9 YGL166W CUP2 hom Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; CUP2 has a paralog, HAA1, that arose from the whole genome duplication
0.100 7.00E-9 YBL002W HTB2 hom Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.095 3.39E-8 YJL132W_p YJL132W_p hom Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene
0.088 2.97E-7 YDR503C LPP1 hom Lipid phosphate phosphatase, catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA
0.088 2.98E-7 YDR423C CAD1 hom AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication
0.087 4.61E-7 YEL072W RMD6 hom Protein required for sporulation
0.086 6.41E-7 YBR284W_p YBR284W_p hom Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication
0.086 7.00E-7 YPR078C_p YPR078C_p hom Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible
0.085 7.34E-7 YOR037W CYC2 hom Mitochondrial peripheral inner membrane protein, contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
0.085 9.43E-7 YGL082W_p YGL082W_p hom Putative protein of unknown function; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YGL082W is not an essential gene
0.084 1.26E-6 YNL094W APP1 hom Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway