YLR300W / EXG1

Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR300W / EXG1

Click on Significant Values for Screen Details ID:SGTC_245|Compound:methotrexate|FD-Score:2.95|P-value:7.81E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.58|P-value:6.55E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:2.90|P-value:9.36E-4 ID:SGTC_397|Compound:arbidol|FD-Score:2.97|P-value:7.37E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.06|P-value:5.26E-4 ID:SGTC_687|Compound:k029-0044|FD-Score:3.58|P-value:6.57E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:4.16|P-value:4.38E-6 ID:SGTC_775|Compound:4577-0580|FD-Score:3.04|P-value:5.80E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.33|P-value:1.84E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.15|P-value:4.11E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:3.40|P-value:1.40E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.56|P-value:5.57E-7 ID:SGTC_1045|Compound:1348-1485|FD-Score:-4.47|P-value:1.03E-6 ID:SGTC_1048|Compound:3161-0300|FD-Score:3.03|P-value:5.83E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:-2.92|P-value:9.71E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:-3.98|P-value:1.19E-5 ID:SGTC_1058|Compound:3448-5425|FD-Score:3.96|P-value:1.13E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.73|P-value:3.25E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.11|P-value:4.41E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:2.94|P-value:8.32E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.16|P-value:4.03E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-3.52|P-value:9.53E-5 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.33|P-value:1.85E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:-3.45|P-value:1.29E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.39|P-value:1.42E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-2.98|P-value:7.77E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.16|P-value:3.65E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.43|P-value:1.20E-4 ID:SGTC_1901|Compound:st060216|FD-Score:2.96|P-value:7.61E-4 ID:SGTC_2065|Compound:5221596|FD-Score:3.04|P-value:5.79E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.46|P-value:1.22E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:-2.92|P-value:9.89E-4 ID:SGTC_2208|Compound:7283366|FD-Score:3.21|P-value:2.92E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.07|P-value:5.65E-4 ID:SGTC_2265|Compound:7929201|FD-Score:-3.10|P-value:4.98E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.26|P-value:2.44E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.04|P-value:6.45E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.65|P-value:4.80E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.55|P-value:7.24E-5 ID:SGTC_2445|Compound:5742597|FD-Score:-3.99|P-value:1.13E-5 ID:SGTC_2451|Compound:5856223|FD-Score:-3.02|P-value:6.92E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.05|P-value:5.55E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.07|P-value:5.09E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.01|P-value:6.35E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-3.22|P-value:3.15E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.35|P-value:1.69E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.58|P-value:6.46E-5 ID:SGTC_2645|Compound:abamectin|FD-Score:2.94|P-value:8.11E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:3.07|P-value:5.07E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.89|P-value:8.96E-8 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.08|P-value:5.53E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.29|P-value:2.12E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.81|P-value:2.60E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:2.95|P-value:7.81E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.58|P-value:6.55E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:2.90|P-value:9.36E-4 ID:SGTC_397|Compound:arbidol|FD-Score:2.97|P-value:7.37E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.06|P-value:5.26E-4 ID:SGTC_687|Compound:k029-0044|FD-Score:3.58|P-value:6.57E-5 ID:SGTC_769|Compound:0302-0231|FD-Score:4.16|P-value:4.38E-6 ID:SGTC_775|Compound:4577-0580|FD-Score:3.04|P-value:5.80E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.33|P-value:1.84E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.15|P-value:4.11E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:3.40|P-value:1.40E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.56|P-value:5.57E-7 ID:SGTC_1045|Compound:1348-1485|FD-Score:-4.47|P-value:1.03E-6 ID:SGTC_1048|Compound:3161-0300|FD-Score:3.03|P-value:5.83E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:-2.92|P-value:9.71E-4 ID:SGTC_1051|Compound:3253-3903|FD-Score:-3.98|P-value:1.19E-5 ID:SGTC_1058|Compound:3448-5425|FD-Score:3.96|P-value:1.13E-5 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.73|P-value:3.25E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.11|P-value:4.41E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:2.94|P-value:8.32E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-3.16|P-value:4.03E-4 ID:SGTC_1329|Compound:1447-1697|FD-Score:-3.52|P-value:9.53E-5 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.33|P-value:1.85E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:-3.45|P-value:1.29E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:3.39|P-value:1.42E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:-2.98|P-value:7.77E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.16|P-value:3.65E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.43|P-value:1.20E-4 ID:SGTC_1901|Compound:st060216|FD-Score:2.96|P-value:7.61E-4 ID:SGTC_2065|Compound:5221596|FD-Score:3.04|P-value:5.79E-4 ID:SGTC_2146|Compound:5545642|FD-Score:-3.46|P-value:1.22E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:-2.92|P-value:9.89E-4 ID:SGTC_2208|Compound:7283366|FD-Score:3.21|P-value:2.92E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.07|P-value:5.65E-4 ID:SGTC_2265|Compound:7929201|FD-Score:-3.10|P-value:4.98E-4 ID:SGTC_2324|Compound:6149169|FD-Score:3.26|P-value:2.44E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.04|P-value:6.45E-4 ID:SGTC_2437|Compound:5741531|FD-Score:3.65|P-value:4.80E-5 ID:SGTC_2440|Compound:5628896|FD-Score:3.55|P-value:7.24E-5 ID:SGTC_2445|Compound:5742597|FD-Score:-3.99|P-value:1.13E-5 ID:SGTC_2451|Compound:5856223|FD-Score:-3.02|P-value:6.92E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.05|P-value:5.55E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.07|P-value:5.09E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:3.01|P-value:6.35E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-3.22|P-value:3.15E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:3.35|P-value:1.69E-4 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.58|P-value:6.46E-5 ID:SGTC_2645|Compound:abamectin|FD-Score:2.94|P-value:8.11E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:3.07|P-value:5.07E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:4.89|P-value:8.96E-8 ID:SGTC_2774|Compound:tamoxifen|FD-Score:-3.08|P-value:5.53E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.29|P-value:2.12E-4 ID:SGTC_2922|Compound:7999257|FD-Score:-3.81|P-value:2.60E-5

Top fitness defect scores for YLR300W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2772 vinpocetine 4.89 57.1 µM 8.96E-8 12 5.16E-7
2 SGTC_1000 4048-0884 4.56 8.7 µM 5.57E-7 copper-dependent oxidative stress 22 2.59E-6
3 SGTC_769 0302-0231 4.16 76.0 µM 4.38E-6 32 1.60E-5
4 SGTC_1058 3448-5425 3.96 97.3 µM 1.13E-5 34 3.70E-5
5 SGTC_1132 3456-3614 3.73 2.5 µM 3.25E-5 tubulin folding & SWR complex 63 9.39E-5
6 SGTC_2437 5741531 3.65 144.2 µM 4.80E-5 47 1.33E-4
7 SGTC_2633 5,7-dihydroxyisoflavone 3.58 100.0 µM 6.46E-5 40 1.72E-4
8 SGTC_249 wiskostatin 3.58 11.2 µM 6.55E-5 21 1.74E-4
9 SGTC_687 k029-0044 3.58 34.9 µM 6.57E-5 PDR1 27 1.75E-4
10 SGTC_2440 5628896 3.55 157.8 µM 7.24E-5 33 1.91E-4
11 SGTC_1891 st059073 3.43 26.2 µM 1.20E-4 TSC3-RPN4 33 2.99E-4
12 SGTC_945 3381-0768 3.40 11.3 µM 1.40E-4 endomembrane recycling 48 3.41E-4
13 SGTC_1445 4239-0156 3.39 97.7 µM 1.42E-4 50 3.47E-4
14 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 3.35 100.0 µM 1.69E-4 60 4.04E-4
15 SGTC_871 0574-0004 3.33 149.0 µM 1.84E-4 72 4.36E-4
16 SGTC_1360 1598-0022 3.33 39.8 µM 1.85E-4 ERG2 57 4.38E-4
17 SGTC_2825 7998094 3.29 58.4 µM 2.12E-4 fatty acid desaturase (OLE1) 53 4.93E-4
18 SGTC_2324 6149169 3.26 200.0 µM 2.44E-4 38 5.58E-4
19 SGTC_2208 7283366 3.21 200.0 µM 2.92E-4 25 6.55E-4
20 SGTC_1719 st032227 3.16 49.2 µM 3.65E-4 57 7.97E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 6.47E-13 YDR233C RTN1 hom Reticulon protein; stabilizes membrane curvature; involved in nuclear pore assembly and maintenance of tubular ER morphology; mutant overexpressing RTN1 shows increase in tubular ER; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; more abundant than Rtn2p; member of the RTNLA subfamily; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; RTN1 has a paralog, RTN2, that arose from the whole genome duplication
0.114 4.27E-11 YKL147C_d YKL147C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3
0.108 3.26E-10 YER009W NTF2 het Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
0.105 9.03E-10 YLR082C SRL2 hom Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
0.105 1.22E-9 YGR001C_p YGR001C_p hom Putative protein of unknown function with similarity to methyltransferase family members; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in S. cerevisiae
0.102 3.46E-9 YLR247C IRC20 hom Putative helicase; localizes to the mitochondrion and the nucleus; YLR247C is not an essential gene; null mutant displays increased levels of spontaneous Rad52p foci
0.100 7.04E-9 YOR285W RDL1 hom Protein of unknown function containing a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress
0.099 7.85E-9 YMR115W MGR3 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
0.094 4.39E-8 YBL045C COR1 hom Core subunit of the ubiquinol-cytochrome c reductase complex (bc1 complex), which is a component of the mitochondrial inner membrane electron transport chain
0.091 1.23E-7 YDR104C SPO71 hom Meiosis-specific protein of unknown function, required for spore wall formation during sporulation, in particular for the proper size of the prospore membrane (PSM); interacts with SPO1; dispensable for both nuclear divisions during meiosis; contains two PH domains
0.089 2.72E-7 YHR101C BIG1 het Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan
0.088 3.53E-7 YIL168W YIL168W hom Open reading frame, unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase
0.086 6.01E-7 YHR040W BCD1 het Essential protein required for the accumulation of box C/D snoRNA
0.085 8.65E-7 YNL338W_d YNL338W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is Telomeric X element Core sequence on the left arm of Chromosome XIV
0.084 9.77E-7 YBR083W TEC1 hom Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member