YMR048W / CSM3

Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR048W / CSM3

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:2.95|P-value:6.61E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:-3.08|P-value:4.90E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.64|P-value:2.34E-7 ID:SGTC_377|Compound:3970-0964|FD-Score:3.66|P-value:3.47E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-2.95|P-value:8.09E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:3.58|P-value:5.00E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.74|P-value:3.12E-5 ID:SGTC_507|Compound:nimesulide|FD-Score:-4.35|P-value:1.51E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:4.66|P-value:2.10E-7 ID:SGTC_558|Compound:k064-0027|FD-Score:4.99|P-value:3.12E-8 ID:SGTC_624|Compound:k048-0052|FD-Score:-3.03|P-value:5.92E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:2.91|P-value:7.74E-4 ID:SGTC_681|Compound:1642-0006|FD-Score:-2.92|P-value:9.10E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-6.75|P-value:1.98E-13 ID:SGTC_829|Compound:1635-0120|FD-Score:3.14|P-value:3.14E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.16|P-value:3.06E-6 ID:SGTC_1060|Compound:3448-7350|FD-Score:3.00|P-value:5.51E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.28|P-value:1.77E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.04|P-value:4.60E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.38|P-value:1.16E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-4.44|P-value:9.57E-7 ID:SGTC_1265|Compound:0819-1001|FD-Score:-6.80|P-value:1.38E-13 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.10|P-value:3.73E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:2.97|P-value:6.03E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:-3.60|P-value:5.74E-5 ID:SGTC_1707|Compound:st032323|FD-Score:8.81|P-value:6.99E-22 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-4.03|P-value:8.06E-6 ID:SGTC_2237|Compound:6667747|FD-Score:2.87|P-value:8.92E-4 ID:SGTC_2413|Compound:inermin|FD-Score:2.99|P-value:5.58E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:-4.63|P-value:3.33E-7 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.27|P-value:2.32E-6 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.26|P-value:2.46E-4 ID:SGTC_2444|Compound:5485484|FD-Score:2.99|P-value:5.55E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.16|P-value:3.16E-6 ID:SGTC_2706|Compound:st078239|FD-Score:-3.71|P-value:3.58E-5 ID:SGTC_2780|Compound:5215298|FD-Score:5.38|P-value:2.72E-9 ID:SGTC_2785|Compound:7756463|FD-Score:3.07|P-value:4.14E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.13|P-value:4.03E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.09|P-value:4.84E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:2.95|P-value:6.61E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:-3.08|P-value:4.90E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.64|P-value:2.34E-7 ID:SGTC_377|Compound:3970-0964|FD-Score:3.66|P-value:3.47E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-2.95|P-value:8.09E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:3.58|P-value:5.00E-5 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.74|P-value:3.12E-5 ID:SGTC_507|Compound:nimesulide|FD-Score:-4.35|P-value:1.51E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:4.66|P-value:2.10E-7 ID:SGTC_558|Compound:k064-0027|FD-Score:4.99|P-value:3.12E-8 ID:SGTC_624|Compound:k048-0052|FD-Score:-3.03|P-value:5.92E-4 ID:SGTC_634|Compound:1611-4317|FD-Score:2.91|P-value:7.74E-4 ID:SGTC_681|Compound:1642-0006|FD-Score:-2.92|P-value:9.10E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-6.75|P-value:1.98E-13 ID:SGTC_829|Compound:1635-0120|FD-Score:3.14|P-value:3.14E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.16|P-value:3.06E-6 ID:SGTC_1060|Compound:3448-7350|FD-Score:3.00|P-value:5.51E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.28|P-value:1.77E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.04|P-value:4.60E-4 ID:SGTC_1183|Compound:1254-0324|FD-Score:3.38|P-value:1.16E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-4.44|P-value:9.57E-7 ID:SGTC_1265|Compound:0819-1001|FD-Score:-6.80|P-value:1.38E-13 ID:SGTC_1326|Compound:1431-2094|FD-Score:3.10|P-value:3.73E-4 ID:SGTC_1343|Compound:1487-0095|FD-Score:2.97|P-value:6.03E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:-3.60|P-value:5.74E-5 ID:SGTC_1707|Compound:st032323|FD-Score:8.81|P-value:6.99E-22 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-4.03|P-value:8.06E-6 ID:SGTC_2237|Compound:6667747|FD-Score:2.87|P-value:8.92E-4 ID:SGTC_2413|Compound:inermin|FD-Score:2.99|P-value:5.58E-4 ID:SGTC_2417|Compound:cerulenin|FD-Score:-4.63|P-value:3.33E-7 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.27|P-value:2.32E-6 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.26|P-value:2.46E-4 ID:SGTC_2444|Compound:5485484|FD-Score:2.99|P-value:5.55E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.16|P-value:3.16E-6 ID:SGTC_2706|Compound:st078239|FD-Score:-3.71|P-value:3.58E-5 ID:SGTC_2780|Compound:5215298|FD-Score:5.38|P-value:2.72E-9 ID:SGTC_2785|Compound:7756463|FD-Score:3.07|P-value:4.14E-4 ID:SGTC_2945|Compound:9067681|FD-Score:-3.13|P-value:4.03E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.09|P-value:4.84E-4

Top fitness defect scores for YMR048W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1707 st032323 8.81 6.3 µM 6.99E-22 DNA damage response 12 5.99E-19
2 SGTC_2780 5215298 5.38 71.4 µM 2.72E-9 12 3.78E-8
3 SGTC_558 k064-0027 4.99 21.3 µM 3.12E-8 DNA damage response 13 3.06E-7
4 SGTC_514 4466-0038 4.66 2.6 nM 2.10E-7 cell wall 123 1.58E-6
5 SGTC_268 camptothecin 4.64 39.6 µM 2.34E-7 DNA damage response 52 1.73E-6
6 SGTC_964 1287-0107 4.16 19.4 µM 3.06E-6 cell wall 33 1.57E-5
7 SGTC_2584 androsterone 4.16 48.7 µM 3.16E-6 cell wall 121 1.61E-5
8 SGTC_377 3970-0964 3.66 33.1 µM 3.47E-5 49 1.27E-4
9 SGTC_455 paf C-16 3.58 10.0 µM 5.00E-5 40 1.73E-4
10 SGTC_1183 1254-0324 3.38 198.0 µM 1.16E-4 44 3.58E-4
11 SGTC_1079 idarubicin 3.28 2.4 µM 1.77E-4 DNA damage response 37 5.14E-4
12 SGTC_829 1635-0120 3.14 117.0 µM 3.14E-4 DNA damage response 73 8.43E-4
13 SGTC_1326 1431-2094 3.10 10.8 µM 3.73E-4 tubulin folding & SWR complex 66 9.77E-4
14 SGTC_2785 7756463 3.07 72.7 µM 4.14E-4 75 0.00107
15 SGTC_1118 chelidonine 3.04 707.5 nM 4.60E-4 55 0.00117
16 SGTC_1060 3448-7350 3.00 82.0 µM 5.51E-4 Golgi 66 0.00137
17 SGTC_2444 5485484 2.99 36.4 µM 5.55E-4 83 0.00138
18 SGTC_2413 inermin 2.99 26.1 µM 5.58E-4 101 0.00138
19 SGTC_1343 1487-0095 2.97 224.0 µM 6.03E-4 35 0.00148
20 SGTC_67 0335-0847 2.95 266.0 µM 6.61E-4 ubiquinone biosynthesis & proteasome 192 0.00160

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.316 1.40E-78 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.277 3.57E-60 YNL273W TOF1 hom Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase
0.264 1.40E-54 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.248 4.11E-48 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.225 7.39E-40 YDR075W PPH3 hom Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
0.214 5.15E-36 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.210 7.80E-35 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.200 1.34E-31 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.194 7.84E-30 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.190 9.74E-29 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.190 1.18E-28 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.190 1.54E-28 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.181 4.42E-26 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.174 2.43E-24 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.170 2.78E-23 YOR191W ULS1 hom Protein involved in proteolytic control of sumoylated substrates; contains RING finger domain; interacts with SUMO (Smt3p); member of the SWI/SNF family of DNA-dependent ATPases; plays a role in antagonizing silencing during mating-type switching; relocalizes from nucleus to cytoplasm upon DNA replication stress