YMR186W / HSC82

Cytoplasmic chaperone of the Hsp90 family; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR186W / HSC82

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:3.13|P-value:8.78E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:3.13|P-value:8.95E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:-3.10|P-value:9.11E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.62|P-value:1.48E-4 ID:SGTC_394|Compound:menadione|FD-Score:3.63|P-value:1.40E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.10|P-value:9.66E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:4.22|P-value:1.22E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.31|P-value:4.40E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-6.02|P-value:7.65E-10 ID:SGTC_535|Compound:2293-4129|FD-Score:4.00|P-value:3.16E-5 ID:SGTC_907|Compound:3448-6986|FD-Score:3.69|P-value:1.14E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.29|P-value:5.13E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.71|P-value:1.03E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:3.38|P-value:3.70E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.15|P-value:1.67E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.25|P-value:5.91E-4 ID:SGTC_1771|Compound:st048882|FD-Score:4.28|P-value:9.55E-6 ID:SGTC_1801|Compound:st050089|FD-Score:3.44|P-value:2.88E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.21|P-value:6.71E-4 ID:SGTC_1887|Compound:st057648|FD-Score:3.69|P-value:1.12E-4 ID:SGTC_1897|Compound:st060201|FD-Score:3.10|P-value:9.92E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.71|P-value:1.05E-4 ID:SGTC_1991|Compound:st073000|FD-Score:-3.18|P-value:6.87E-4 ID:SGTC_2043|Compound:5101342|FD-Score:3.18|P-value:7.49E-4 ID:SGTC_2071|Compound:5230951|FD-Score:3.60|P-value:1.62E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.45|P-value:2.67E-4 ID:SGTC_2218|Compound:6965428|FD-Score:3.36|P-value:3.94E-4 ID:SGTC_2376|Compound:9072922|FD-Score:4.21|P-value:1.25E-5 ID:SGTC_2465|Compound:5809242|FD-Score:3.59|P-value:1.67E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.66|P-value:1.27E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-5.88|P-value:1.84E-9 ID:SGTC_2803|Compound:7707263|FD-Score:-5.11|P-value:1.48E-7 ID:SGTC_2846|Compound:9008642|FD-Score:3.50|P-value:2.33E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.46|P-value:4.03E-6 ID:SGTC_3030|Compound:9087619|FD-Score:-3.57|P-value:1.69E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.33|P-value:4.36E-4 ID:SGTC_3340|Compound:9146634|FD-Score:3.63|P-value:1.41E-4 ID:SGTC_3348|Compound:9149464|FD-Score:3.23|P-value:6.16E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:3.13|P-value:8.78E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:3.13|P-value:8.95E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:-3.10|P-value:9.11E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.62|P-value:1.48E-4 ID:SGTC_394|Compound:menadione|FD-Score:3.63|P-value:1.40E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:3.10|P-value:9.66E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:4.22|P-value:1.22E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.31|P-value:4.40E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-6.02|P-value:7.65E-10 ID:SGTC_535|Compound:2293-4129|FD-Score:4.00|P-value:3.16E-5 ID:SGTC_907|Compound:3448-6986|FD-Score:3.69|P-value:1.14E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.29|P-value:5.13E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.71|P-value:1.03E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:3.38|P-value:3.70E-4 ID:SGTC_1417|Compound:3966-0327|FD-Score:4.15|P-value:1.67E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.25|P-value:5.91E-4 ID:SGTC_1771|Compound:st048882|FD-Score:4.28|P-value:9.55E-6 ID:SGTC_1801|Compound:st050089|FD-Score:3.44|P-value:2.88E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.21|P-value:6.71E-4 ID:SGTC_1887|Compound:st057648|FD-Score:3.69|P-value:1.12E-4 ID:SGTC_1897|Compound:st060201|FD-Score:3.10|P-value:9.92E-4 ID:SGTC_1934|Compound:st074720|FD-Score:3.71|P-value:1.05E-4 ID:SGTC_1991|Compound:st073000|FD-Score:-3.18|P-value:6.87E-4 ID:SGTC_2043|Compound:5101342|FD-Score:3.18|P-value:7.49E-4 ID:SGTC_2071|Compound:5230951|FD-Score:3.60|P-value:1.62E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.45|P-value:2.67E-4 ID:SGTC_2218|Compound:6965428|FD-Score:3.36|P-value:3.94E-4 ID:SGTC_2376|Compound:9072922|FD-Score:4.21|P-value:1.25E-5 ID:SGTC_2465|Compound:5809242|FD-Score:3.59|P-value:1.67E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.66|P-value:1.27E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-5.88|P-value:1.84E-9 ID:SGTC_2803|Compound:7707263|FD-Score:-5.11|P-value:1.48E-7 ID:SGTC_2846|Compound:9008642|FD-Score:3.50|P-value:2.33E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.46|P-value:4.03E-6 ID:SGTC_3030|Compound:9087619|FD-Score:-3.57|P-value:1.69E-4 ID:SGTC_3338|Compound:9144300|FD-Score:3.33|P-value:4.36E-4 ID:SGTC_3340|Compound:9146634|FD-Score:3.63|P-value:1.41E-4 ID:SGTC_3348|Compound:9149464|FD-Score:3.23|P-value:6.16E-4

Top fitness defect scores for YMR186W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2981 9013952 4.46 39.0 µM 4.03E-6 32 4.04E-6
2 SGTC_1771 st048882 4.28 4.2 µM 9.55E-6 RPP1 & pyrimidine depletion 12 9.54E-6
3 SGTC_455 paf C-16 4.22 10.0 µM 1.22E-5 27 1.22E-5
4 SGTC_2376 9072922 4.21 81.3 µM 1.25E-5 18 1.25E-5
5 SGTC_1417 3966-0327 4.15 90.4 µM 1.67E-5 12 1.67E-5
6 SGTC_535 2293-4129 4.00 48.2 µM 3.16E-5 NEO1 59 3.15E-5
7 SGTC_1236 0330-0013 3.71 258.0 µM 1.03E-4 16 1.02E-4
8 SGTC_1934 st074720 3.71 32.6 µM 1.05E-4 65 1.04E-4
9 SGTC_1887 st057648 3.69 70.8 µM 1.12E-4 25 1.12E-4
10 SGTC_907 3448-6986 3.69 75.5 µM 1.14E-4 34 1.14E-4
11 SGTC_2596 sinensetin 3.66 100.0 µM 1.27E-4 41 1.27E-4
12 SGTC_394 menadione 3.63 8.3 µM 1.40E-4 superoxide 44 1.39E-4
13 SGTC_3340 9146634 3.63 11.1 µM 1.41E-4 21 1.40E-4
14 SGTC_194 1486-1323 3.62 56.9 µM 1.48E-4 27 1.47E-4
15 SGTC_2071 5230951 3.60 39.9 µM 1.62E-4 TSC3-RPN4 29 1.61E-4
16 SGTC_2465 5809242 3.59 33.2 µM 1.67E-4 6 1.66E-4
17 SGTC_2846 9008642 3.50 19.5 µM 2.33E-4 25 2.31E-4
18 SGTC_1801 st050089 3.44 28.4 µM 2.88E-4 NEO1-PIK1 151 2.86E-4
19 SGTC_1394 3474-0001 3.38 65.7 µM 3.70E-4 endomembrane recycling 58 3.67E-4
20 SGTC_2218 6965428 3.36 193.0 µM 3.94E-4 TSC3-RPN4 16 3.91E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.114 3.20E-11 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.089 2.22E-7 YGL151W NUT1 hom Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription
0.087 4.71E-7 YGR178C PBP1 hom Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; similar to human ataxin-2; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
0.082 1.77E-6 YLL030C_d RRT7_d hom Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.082 1.98E-6 YMR017W SPO20 hom Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog
0.081 2.50E-6 YCR013C_d YCR013C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YCR013C and the overlapping essential gene PGK1 is reduced in a gcr1 null mutant
0.080 3.94E-6 YLR142W PUT1 hom Proline oxidase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
0.079 4.03E-6 YDR093W DNF2 hom Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
0.076 1.18E-5 YML036W CGI121 hom Component of the EKC/KEOPS complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription; Cgi121p is dispensable for tRNA modification
0.073 2.10E-5 YIL127C_p RRT14_p hom Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
0.073 2.17E-5 YIL095W PRK1 hom Protein serine/threonine kinase; regulates the organization and function of the actin cytoskeleton and reduces endocytic ability of cell through the phosphorylation of the Pan1p-Sla1p-End3p protein complex; PRK1 has a paralog, ARK1, that arose from the whole genome duplication
0.072 2.79E-5 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.072 2.88E-5 YER120W SCS2 hom Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
0.072 3.11E-5 YNL086W_p SNN1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to endosomes
0.071 4.23E-5 YCL044C MGR1 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA