YMR228W / MTF1

Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation

Zygosity: Homozygous strain
fixedexpanded
Profile for YMR228W / MTF1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:4.00|P-value:2.11E-5 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.44|P-value:2.88E-6 ID:SGTC_63|Compound:0099-0242|FD-Score:6.20|P-value:1.32E-10 ID:SGTC_68|Compound:0986-0284|FD-Score:4.08|P-value:1.48E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:3.47|P-value:1.87E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:3.11|P-value:7.08E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.28|P-value:3.90E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:11.70|P-value:7.41E-33 ID:SGTC_236|Compound:1509-0011|FD-Score:11.20|P-value:3.52E-30 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.55|P-value:1.68E-6 ID:SGTC_283|Compound:0987-0079|FD-Score:3.40|P-value:2.52E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:13.50|P-value:7.15E-43 ID:SGTC_314|Compound:1671-0008|FD-Score:5.05|P-value:1.24E-7 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.40|P-value:3.21E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:3.03|P-value:9.45E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-3.30|P-value:4.61E-4 ID:SGTC_444|Compound:curcumin|FD-Score:-3.48|P-value:2.35E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.17|P-value:2.55E-10 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.42|P-value:2.97E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:4.07|P-value:1.57E-5 ID:SGTC_554|Compound:4483-2211|FD-Score:3.67|P-value:8.67E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:3.33|P-value:3.17E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.52|P-value:8.80E-9 ID:SGTC_802|Compound:4112-3352|FD-Score:3.56|P-value:1.31E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:6.38|P-value:3.91E-11 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.61|P-value:1.11E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.37|P-value:5.45E-6 ID:SGTC_1159|Compound:4092-0538|FD-Score:4.52|P-value:1.91E-6 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.89|P-value:4.68E-5 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.81|P-value:4.90E-5 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.17|P-value:5.71E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.30|P-value:3.65E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.49|P-value:1.75E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.40|P-value:3.21E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.46|P-value:2.60E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.90|P-value:3.37E-5 ID:SGTC_1627|Compound:st003713|FD-Score:3.74|P-value:6.49E-5 ID:SGTC_1707|Compound:st032323|FD-Score:-3.15|P-value:7.82E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-7.76|P-value:2.46E-15 ID:SGTC_1719|Compound:st032227|FD-Score:3.07|P-value:8.29E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.53|P-value:1.98E-4 ID:SGTC_1730|Compound:st033808|FD-Score:3.09|P-value:7.75E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-6.61|P-value:1.27E-11 ID:SGTC_1742|Compound:st038235|FD-Score:6.49|P-value:1.87E-11 ID:SGTC_1769|Compound:st044406|FD-Score:3.13|P-value:6.76E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.94|P-value:3.35E-7 ID:SGTC_1818|Compound:st052136|FD-Score:-3.34|P-value:4.02E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.63|P-value:1.02E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-3.33|P-value:4.11E-4 ID:SGTC_2033|Compound:5192550|FD-Score:3.16|P-value:6.01E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.53|P-value:1.48E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:4.11|P-value:1.31E-5 ID:SGTC_2264|Compound:7948757|FD-Score:3.84|P-value:4.24E-5 ID:SGTC_2275|Compound:7947463|FD-Score:-3.14|P-value:8.14E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-3.28|P-value:5.09E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.76|P-value:7.94E-5 ID:SGTC_2399|Compound:5884133|FD-Score:-3.49|P-value:2.33E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.61|P-value:1.08E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.93|P-value:2.39E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.03|P-value:9.53E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-8.43|P-value:8.23E-18 ID:SGTC_2586|Compound:curcumin|FD-Score:-5.55|P-value:1.11E-8 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.07|P-value:8.31E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.23|P-value:4.71E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:-4.04|P-value:2.49E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:-7.87|P-value:9.59E-16 ID:SGTC_2778|Compound:tioconazole|FD-Score:-5.15|P-value:1.07E-7 ID:SGTC_2805|Compound:7950783|FD-Score:4.47|P-value:2.52E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-6.50|P-value:2.79E-11 ID:SGTC_2978|Compound:9011784|FD-Score:3.05|P-value:8.74E-4 ID:SGTC_3045|Compound:9092950|FD-Score:3.06|P-value:8.59E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.38|P-value:3.45E-4 ID:SGTC_3207|Compound:9114163|FD-Score:4.56|P-value:1.59E-6 ID:SGTC_3208|Compound:9114115|FD-Score:7.92|P-value:3.64E-16 ID:SGTC_10|Compound:0279-0212|FD-Score:4.00|P-value:2.11E-5 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:4.44|P-value:2.88E-6 ID:SGTC_63|Compound:0099-0242|FD-Score:6.20|P-value:1.32E-10 ID:SGTC_68|Compound:0986-0284|FD-Score:4.08|P-value:1.48E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:3.47|P-value:1.87E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:3.11|P-value:7.08E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.28|P-value:3.90E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:11.70|P-value:7.41E-33 ID:SGTC_236|Compound:1509-0011|FD-Score:11.20|P-value:3.52E-30 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.55|P-value:1.68E-6 ID:SGTC_283|Compound:0987-0079|FD-Score:3.40|P-value:2.52E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:13.50|P-value:7.15E-43 ID:SGTC_314|Compound:1671-0008|FD-Score:5.05|P-value:1.24E-7 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.40|P-value:3.21E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:3.03|P-value:9.45E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-3.30|P-value:4.61E-4 ID:SGTC_444|Compound:curcumin|FD-Score:-3.48|P-value:2.35E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.17|P-value:2.55E-10 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.42|P-value:2.97E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:4.07|P-value:1.57E-5 ID:SGTC_554|Compound:4483-2211|FD-Score:3.67|P-value:8.67E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:3.33|P-value:3.17E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:5.52|P-value:8.80E-9 ID:SGTC_802|Compound:4112-3352|FD-Score:3.56|P-value:1.31E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:6.38|P-value:3.91E-11 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.61|P-value:1.11E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.37|P-value:5.45E-6 ID:SGTC_1159|Compound:4092-0538|FD-Score:4.52|P-value:1.91E-6 ID:SGTC_1161|Compound:k007-0610|FD-Score:-3.89|P-value:4.68E-5 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.81|P-value:4.90E-5 ID:SGTC_1228|Compound:0124-0032|FD-Score:3.17|P-value:5.71E-4 ID:SGTC_1257|Compound:0669-0121|FD-Score:3.30|P-value:3.65E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:3.49|P-value:1.75E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.40|P-value:3.21E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.46|P-value:2.60E-4 ID:SGTC_1623|Compound:st003709|FD-Score:3.90|P-value:3.37E-5 ID:SGTC_1627|Compound:st003713|FD-Score:3.74|P-value:6.49E-5 ID:SGTC_1707|Compound:st032323|FD-Score:-3.15|P-value:7.82E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-7.76|P-value:2.46E-15 ID:SGTC_1719|Compound:st032227|FD-Score:3.07|P-value:8.29E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-3.53|P-value:1.98E-4 ID:SGTC_1730|Compound:st033808|FD-Score:3.09|P-value:7.75E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-6.61|P-value:1.27E-11 ID:SGTC_1742|Compound:st038235|FD-Score:6.49|P-value:1.87E-11 ID:SGTC_1769|Compound:st044406|FD-Score:3.13|P-value:6.76E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.94|P-value:3.35E-7 ID:SGTC_1818|Compound:st052136|FD-Score:-3.34|P-value:4.02E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:3.63|P-value:1.02E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-3.33|P-value:4.11E-4 ID:SGTC_2033|Compound:5192550|FD-Score:3.16|P-value:6.01E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.53|P-value:1.48E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:4.11|P-value:1.31E-5 ID:SGTC_2264|Compound:7948757|FD-Score:3.84|P-value:4.24E-5 ID:SGTC_2275|Compound:7947463|FD-Score:-3.14|P-value:8.14E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-3.28|P-value:5.09E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.76|P-value:7.94E-5 ID:SGTC_2399|Compound:5884133|FD-Score:-3.49|P-value:2.33E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.61|P-value:1.08E-4 ID:SGTC_2526|Compound:patulin|FD-Score:4.93|P-value:2.39E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.03|P-value:9.53E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-8.43|P-value:8.23E-18 ID:SGTC_2586|Compound:curcumin|FD-Score:-5.55|P-value:1.11E-8 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.07|P-value:8.31E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.23|P-value:4.71E-4 ID:SGTC_2776|Compound:terbinafine|FD-Score:-4.04|P-value:2.49E-5 ID:SGTC_2777|Compound:climbazole|FD-Score:-7.87|P-value:9.59E-16 ID:SGTC_2778|Compound:tioconazole|FD-Score:-5.15|P-value:1.07E-7 ID:SGTC_2805|Compound:7950783|FD-Score:4.47|P-value:2.52E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-6.50|P-value:2.79E-11 ID:SGTC_2978|Compound:9011784|FD-Score:3.05|P-value:8.74E-4 ID:SGTC_3045|Compound:9092950|FD-Score:3.06|P-value:8.59E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-3.38|P-value:3.45E-4 ID:SGTC_3207|Compound:9114163|FD-Score:4.56|P-value:1.59E-6 ID:SGTC_3208|Compound:9114115|FD-Score:7.92|P-value:3.64E-16

Top fitness defect scores for YMR228W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_310 1273-0058 13.50 1.1 µM 7.15E-43 mitochondrial stress 15 1.50E-41
2 SGTC_173 1273-0061 11.70 2.7 µM 7.41E-33 mitochondrial stress 28 7.82E-32
3 SGTC_236 1509-0011 11.20 5.3 µM 3.52E-30 170 3.09E-29
4 SGTC_3208 9114115 7.92 49.5 µM 3.64E-16 3 1.19E-15
5 SGTC_1742 st038235 6.49 28.9 µM 1.87E-11 mitochondrial stress 35 4.34E-11
6 SGTC_831 k061-1017 6.38 217.0 µM 3.91E-11 translation 8 8.88E-11
7 SGTC_63 0099-0242 6.20 26.7 µM 1.32E-10 mitochondrial stress 26 2.89E-10
8 SGTC_800 3448-7362 5.52 155.0 µM 8.80E-9 translation 66 1.68E-8
9 SGTC_314 1671-0008 5.05 2.7 µM 1.24E-7 20 2.18E-7
10 SGTC_2526 patulin 4.93 37.6 µM 2.39E-7 47 4.10E-7
11 SGTC_3207 9114163 4.56 49.5 µM 1.59E-6 18 2.56E-6
12 SGTC_275 trichlorophene 4.55 5.7 µM 1.68E-6 mitochondrial stress 197 2.70E-6
13 SGTC_1159 4092-0538 4.52 61.2 µM 1.91E-6 16 3.06E-6
14 SGTC_2805 7950783 4.47 58.4 µM 2.52E-6 17 4.00E-6
15 SGTC_28 pentachloropyridine N-oxide 4.44 1.6 µM 2.88E-6 TSC3-RPN4 51 4.55E-6
16 SGTC_2232 chlorophetanol 4.11 41.5 µM 1.31E-5 TRP & mitochondrial translation 116 1.97E-5
17 SGTC_68 0986-0284 4.08 82.8 µM 1.48E-5 66 2.21E-5
18 SGTC_464 bw-b 70c 4.07 158.0 µM 1.57E-5 21 2.35E-5
19 SGTC_10 0279-0212 4.00 42.6 µM 2.11E-5 TRP & mitochondrial translation 117 3.11E-5
20 SGTC_1623 st003709 3.90 20.3 µM 3.37E-5 calcium & mitochondrial duress 77 4.89E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.599 0 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.529 8.97E-242 YPL040C ISM1 hom Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.516 2.38E-227 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit
0.502 2.12E-212 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.494 2.13E-206 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.494 1.29E-205 YER087W AIM10 hom Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
0.493 1.10E-204 YHR168W MTG2 hom Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
0.481 8.31E-194 YDR298C ATP5 hom Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase, which is an evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
0.479 2.39E-192 YNL081C SWS2 hom Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency
0.472 1.15E-185 YKL170W MRPL38 hom Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
0.471 5.61E-185 YLR069C MEF1 hom Mitochondrial elongation factor involved in translational elongation
0.461 4.92E-176 YLL033W IRC19 hom Putative protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci
0.454 1.77E-170 YER077C_p YER077C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
0.453 3.94E-169 YCR046C IMG1 hom Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome
0.452 5.42E-169 YNL177C MRPL22 hom Mitochondrial ribosomal protein of the large subunit