YNL007C / SIS1

Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL007C / SIS1

Click on Significant Values for Screen Details ID:SGTC_30|Compound:5-(2-thienylidene)-Rhodanine|FD-Score:3.03|P-value:2.96E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:3.13|P-value:2.02E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:4.99|P-value:9.61E-9 ID:SGTC_67|Compound:0335-0847|FD-Score:3.46|P-value:4.53E-5 ID:SGTC_701|Compound:4424-0219|FD-Score:2.96|P-value:4.07E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.00|P-value:4.62E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:2.98|P-value:3.67E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.79|P-value:9.26E-6 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.44|P-value:5.11E-5 ID:SGTC_1411|Compound:3909-8223|FD-Score:3.30|P-value:9.38E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-2.94|P-value:5.74E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.31|P-value:8.86E-5 ID:SGTC_1613|Compound:st002381|FD-Score:-2.98|P-value:4.91E-4 ID:SGTC_1624|Compound:st003710|FD-Score:2.88|P-value:5.62E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-2.85|P-value:8.12E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-3.04|P-value:3.85E-4 ID:SGTC_1849|Compound:st056239|FD-Score:-3.84|P-value:1.06E-5 ID:SGTC_2117|Compound:5521686|FD-Score:2.85|P-value:6.23E-4 ID:SGTC_2188|Compound:6126565|FD-Score:4.76|P-value:4.11E-8 ID:SGTC_2315|Compound:7733312|FD-Score:2.89|P-value:5.24E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.46|P-value:4.65E-5 ID:SGTC_2363|Compound:9053361|FD-Score:5.49|P-value:3.23E-10 ID:SGTC_2432|Compound:5630864|FD-Score:-2.82|P-value:9.18E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.84|P-value:6.39E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.53|P-value:3.38E-5 ID:SGTC_2878|Compound:9043973|FD-Score:4.20|P-value:1.05E-6 ID:SGTC_3185|Compound:9108267|FD-Score:-3.19|P-value:2.04E-4 ID:SGTC_3295|Compound:9122362|FD-Score:2.80|P-value:7.61E-4 ID:SGTC_30|Compound:5-(2-thienylidene)-Rhodanine|FD-Score:3.03|P-value:2.96E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:3.13|P-value:2.02E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:4.99|P-value:9.61E-9 ID:SGTC_67|Compound:0335-0847|FD-Score:3.46|P-value:4.53E-5 ID:SGTC_701|Compound:4424-0219|FD-Score:2.96|P-value:4.07E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.00|P-value:4.62E-4 ID:SGTC_873|Compound:0591-4978|FD-Score:2.98|P-value:3.67E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:3.79|P-value:9.26E-6 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.44|P-value:5.11E-5 ID:SGTC_1411|Compound:3909-8223|FD-Score:3.30|P-value:9.38E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-2.94|P-value:5.74E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.31|P-value:8.86E-5 ID:SGTC_1613|Compound:st002381|FD-Score:-2.98|P-value:4.91E-4 ID:SGTC_1624|Compound:st003710|FD-Score:2.88|P-value:5.62E-4 ID:SGTC_1709|Compound:st033179|FD-Score:-2.85|P-value:8.12E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-3.04|P-value:3.85E-4 ID:SGTC_1849|Compound:st056239|FD-Score:-3.84|P-value:1.06E-5 ID:SGTC_2117|Compound:5521686|FD-Score:2.85|P-value:6.23E-4 ID:SGTC_2188|Compound:6126565|FD-Score:4.76|P-value:4.11E-8 ID:SGTC_2315|Compound:7733312|FD-Score:2.89|P-value:5.24E-4 ID:SGTC_2320|Compound:7373003|FD-Score:3.46|P-value:4.65E-5 ID:SGTC_2363|Compound:9053361|FD-Score:5.49|P-value:3.23E-10 ID:SGTC_2432|Compound:5630864|FD-Score:-2.82|P-value:9.18E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:2.84|P-value:6.39E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.53|P-value:3.38E-5 ID:SGTC_2878|Compound:9043973|FD-Score:4.20|P-value:1.05E-6 ID:SGTC_3185|Compound:9108267|FD-Score:-3.19|P-value:2.04E-4 ID:SGTC_3295|Compound:9122362|FD-Score:2.80|P-value:7.61E-4

Top fitness defect scores for YNL007C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2363 9053361 5.49 176.0 µM 3.23E-10 Golgi 5 2.06E-8 0.64
2 SGTC_41 0478-0632 4.99 82.6 µM 9.61E-9 8 3.09E-7 0.00
3 SGTC_2188 6126565 4.76 140.7 µM 4.11E-8 mitochondrial processes 1 9.89E-7 0.35
4 SGTC_2878 9043973 4.20 26.0 µM 1.05E-6 1 1.32E-5 0.89
5 SGTC_1083 didemethylchlorpromazine sulfoxide 3.79 707.4 nM 9.26E-6 redox potentiating 26 7.51E-5 0.00
6 SGTC_2679 rapamycin 3.53 1.0 nM 3.38E-5 59 2.12E-4 0.02
7 SGTC_67 0335-0847 3.46 266.0 µM 4.53E-5 ubiquinone biosynthesis & proteasome 31 2.68E-4 0.18
8 SGTC_2320 7373003 3.46 200.0 µM 4.65E-5 14 2.74E-4 0.06
9 SGTC_1130 3454-2858 3.44 15.9 µM 5.11E-5 NEO1 18 2.95E-4 0.14
10 SGTC_1581 himbacine 3.31 57.9 µM 8.86E-5 Golgi 9 4.58E-4 0.07
11 SGTC_1411 3909-8223 3.30 150.0 µM 9.38E-5 6 4.80E-4 0.24
12 SGTC_32 0928-0142 3.13 34.1 µM 2.02E-4 TSC3-RPN4 14 8.89E-4 0.10
13 SGTC_30 5-(2-thienylidene)-Rhodanine 3.03 28.7 µM 2.96E-4 6 0.00121 0.06
14 SGTC_873 0591-4978 2.98 488.0 µM 3.67E-4 10 0.00143 0.08
15 SGTC_701 4424-0219 2.96 185.0 µM 4.07E-4 calcium & mitochondrial duress 11 0.00156 0.04
16 SGTC_2315 7733312 2.89 200.0 µM 5.24E-4 23 0.00191 0.05
17 SGTC_1624 st003710 2.88 6.2 µM 5.62E-4 calcium & mitochondrial duress 10 0.00202 0.10
18 SGTC_2117 5521686 2.85 200.0 µM 6.23E-4 14 0.00219 0.07
19 SGTC_2583 asiatic acid 2.84 100.0 µM 6.39E-4 27 0.00224 0.03
20 SGTC_3295 9122362 2.80 53.6 µM 7.61E-4 21 0.00258 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.222 7.79E-39 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.186 2.18E-27 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.166 3.56E-22 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.166 3.56E-22 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.152 7.37E-19 YML116W ATR1 hom Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.149 4.70E-18 YPR023C EAF3 hom Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
0.146 1.67E-17 YLR449W FPR4 hom Peptidyl-prolyl cis-trans isomerase (PPIase) (proline isomerase) localized to the nucleus; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones
0.140 4.98E-16 YJR102C VPS25 hom Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome
0.132 1.84E-14 YLR323C CWC24 het Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
0.128 8.96E-14 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.124 5.76E-13 YIL015W BAR1 hom Aspartyl protease secreted into the periplasmic space of mating type a cells, helps cells find mating partners, cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest
0.123 8.14E-13 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.117 1.28E-11 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.116 1.82E-11 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.115 2.34E-11 YOR213C SAS5 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity