YNL256W / FOL1

Multifunctional enzyme of the folic acid biosynthesis pathway, has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities

Zygosity: Heterozygous strain
fixedexpanded
Profile for YNL256W / FOL1

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:-3.65|P-value:1.42E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:2.85|P-value:5.63E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-2.71|P-value:9.64E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:-3.36|P-value:5.91E-5 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.66|P-value:1.41E-5 ID:SGTC_182|Compound:3448-4371|FD-Score:4.43|P-value:2.04E-7 ID:SGTC_225|Compound:nsc-122657|FD-Score:-3.40|P-value:4.94E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:7.18|P-value:1.11E-16 ID:SGTC_293|Compound:4049-0273|FD-Score:3.11|P-value:1.88E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-7.55|P-value:2.72E-18 ID:SGTC_341|Compound:1486-1430|FD-Score:5.46|P-value:2.11E-10 ID:SGTC_350|Compound:k064-0020|FD-Score:5.20|P-value:1.40E-9 ID:SGTC_359|Compound:0081-0058|FD-Score:3.91|P-value:4.03E-6 ID:SGTC_441|Compound:propafenone|FD-Score:4.41|P-value:2.39E-7 ID:SGTC_476|Compound:mead acid|FD-Score:3.66|P-value:1.47E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:-2.86|P-value:5.20E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.26|P-value:9.31E-5 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.60|P-value:1.89E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:2.81|P-value:6.59E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:-2.95|P-value:3.68E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:2.77|P-value:7.89E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:-3.01|P-value:2.75E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:-3.63|P-value:1.56E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.45|P-value:3.88E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.23|P-value:1.14E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:-3.10|P-value:1.94E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:-5.10|P-value:2.60E-9 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.49|P-value:3.11E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.10|P-value:1.99E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.29|P-value:8.57E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:-2.87|P-value:4.98E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.06|P-value:2.42E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:2.75|P-value:8.40E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-5.91|P-value:6.64E-12 ID:SGTC_1855|Compound:st056195|FD-Score:5.31|P-value:6.24E-10 ID:SGTC_1860|Compound:st056228|FD-Score:3.06|P-value:2.36E-4 ID:SGTC_2043|Compound:5101342|FD-Score:3.37|P-value:5.97E-5 ID:SGTC_2055|Compound:5235184|FD-Score:2.84|P-value:5.89E-4 ID:SGTC_2355|Compound:9061122|FD-Score:2.79|P-value:7.17E-4 ID:SGTC_2375|Compound:9072683|FD-Score:-3.29|P-value:7.98E-5 ID:SGTC_2390|Compound:9097792|FD-Score:2.97|P-value:3.41E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.81|P-value:6.70E-6 ID:SGTC_2413|Compound:inermin|FD-Score:2.89|P-value:4.77E-4 ID:SGTC_2415|Compound:st077333|FD-Score:5.52|P-value:1.42E-10 ID:SGTC_2440|Compound:5628896|FD-Score:4.09|P-value:1.48E-6 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.06|P-value:2.40E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.30|P-value:8.09E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:4.03|P-value:2.01E-6 ID:SGTC_2607|Compound:solasodine|FD-Score:2.79|P-value:7.26E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.46|P-value:3.75E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.44|P-value:4.18E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:5.31|P-value:6.40E-10 ID:SGTC_2702|Compound:st077768|FD-Score:-2.72|P-value:9.07E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:5.28|P-value:8.13E-10 ID:SGTC_2819|Compound:7996655|FD-Score:-3.54|P-value:2.52E-5 ID:SGTC_2897|Compound:9048598|FD-Score:-2.76|P-value:7.72E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-2.80|P-value:6.59E-4 ID:SGTC_2974|Compound:9094440|FD-Score:-3.24|P-value:1.04E-4 ID:SGTC_3181|Compound:9108277|FD-Score:3.71|P-value:1.12E-5 ID:SGTC_55|Compound:0767-0285|FD-Score:-3.65|P-value:1.42E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:2.85|P-value:5.63E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:-2.71|P-value:9.64E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:-3.36|P-value:5.91E-5 ID:SGTC_166|Compound:k072-0058|FD-Score:-3.66|P-value:1.41E-5 ID:SGTC_182|Compound:3448-4371|FD-Score:4.43|P-value:2.04E-7 ID:SGTC_225|Compound:nsc-122657|FD-Score:-3.40|P-value:4.94E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:7.18|P-value:1.11E-16 ID:SGTC_293|Compound:4049-0273|FD-Score:3.11|P-value:1.88E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-7.55|P-value:2.72E-18 ID:SGTC_341|Compound:1486-1430|FD-Score:5.46|P-value:2.11E-10 ID:SGTC_350|Compound:k064-0020|FD-Score:5.20|P-value:1.40E-9 ID:SGTC_359|Compound:0081-0058|FD-Score:3.91|P-value:4.03E-6 ID:SGTC_441|Compound:propafenone|FD-Score:4.41|P-value:2.39E-7 ID:SGTC_476|Compound:mead acid|FD-Score:3.66|P-value:1.47E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:-2.86|P-value:5.20E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.26|P-value:9.31E-5 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.60|P-value:1.89E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:2.81|P-value:6.59E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:-2.95|P-value:3.68E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:2.77|P-value:7.89E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:-3.01|P-value:2.75E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:-3.63|P-value:1.56E-5 ID:SGTC_1137|Compound:ixabepilone|FD-Score:-3.45|P-value:3.88E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.23|P-value:1.14E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:-3.10|P-value:1.94E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:-5.10|P-value:2.60E-9 ID:SGTC_1297|Compound:1068-0111|FD-Score:-3.49|P-value:3.11E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.10|P-value:1.99E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:3.29|P-value:8.57E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:-2.87|P-value:4.98E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:3.06|P-value:2.42E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:2.75|P-value:8.40E-4 ID:SGTC_1850|Compound:st056238|FD-Score:-5.91|P-value:6.64E-12 ID:SGTC_1855|Compound:st056195|FD-Score:5.31|P-value:6.24E-10 ID:SGTC_1860|Compound:st056228|FD-Score:3.06|P-value:2.36E-4 ID:SGTC_2043|Compound:5101342|FD-Score:3.37|P-value:5.97E-5 ID:SGTC_2055|Compound:5235184|FD-Score:2.84|P-value:5.89E-4 ID:SGTC_2355|Compound:9061122|FD-Score:2.79|P-value:7.17E-4 ID:SGTC_2375|Compound:9072683|FD-Score:-3.29|P-value:7.98E-5 ID:SGTC_2390|Compound:9097792|FD-Score:2.97|P-value:3.41E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.81|P-value:6.70E-6 ID:SGTC_2413|Compound:inermin|FD-Score:2.89|P-value:4.77E-4 ID:SGTC_2415|Compound:st077333|FD-Score:5.52|P-value:1.42E-10 ID:SGTC_2440|Compound:5628896|FD-Score:4.09|P-value:1.48E-6 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.06|P-value:2.40E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.30|P-value:8.09E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:4.03|P-value:2.01E-6 ID:SGTC_2607|Compound:solasodine|FD-Score:2.79|P-value:7.26E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:-3.46|P-value:3.75E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.44|P-value:4.18E-5 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:5.31|P-value:6.40E-10 ID:SGTC_2702|Compound:st077768|FD-Score:-2.72|P-value:9.07E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:5.28|P-value:8.13E-10 ID:SGTC_2819|Compound:7996655|FD-Score:-3.54|P-value:2.52E-5 ID:SGTC_2897|Compound:9048598|FD-Score:-2.76|P-value:7.72E-4 ID:SGTC_2923|Compound:7992654|FD-Score:-2.80|P-value:6.59E-4 ID:SGTC_2974|Compound:9094440|FD-Score:-3.24|P-value:1.04E-4 ID:SGTC_3181|Compound:9108277|FD-Score:3.71|P-value:1.12E-5

Top fitness defect scores for YNL256W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_245 methotrexate 7.18 289.6 µM 1.11E-16 4 3.48E-13 0.05
2 SGTC_2415 st077333 5.52 57.7 µM 1.42E-10 5 1.71E-8 0.23
3 SGTC_341 1486-1430 5.46 230.0 µM 2.11E-10 9 2.33E-8 0.05
4 SGTC_1855 st056195 5.31 39.6 µM 6.24E-10 60S ribosome export 3 5.37E-8 0.54
5 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 5.31 100.0 µM 6.40E-10 2 5.48E-8 0.63
6 SGTC_2779 methotrexate 5.28 400.0 µM 8.13E-10 8 6.58E-8 0.26
7 SGTC_350 k064-0020 5.20 19.8 µM 1.40E-9 heme requiring 4 1.00E-7 0.07
8 SGTC_182 3448-4371 4.43 550.5 µM 2.04E-7 2 4.66E-6 0.60
9 SGTC_441 propafenone 4.41 146.0 µM 2.39E-7 1 5.27E-6 0.46
10 SGTC_2440 5628896 4.09 157.8 µM 1.48E-6 5 2.16E-5 0.08
11 SGTC_2600 tangeritin 4.03 88.0 µM 2.01E-6 3 2.74E-5 0.44
12 SGTC_359 0081-0058 3.91 15.9 µM 4.03E-6 60S ribosome export 15 4.68E-5 0.11
13 SGTC_2411 st077133 3.81 39.9 µM 6.70E-6 RNA pol III & RNase P/MRP 21 6.94E-5 0.19
14 SGTC_3181 9108277 3.71 49.5 µM 1.12E-5 iron homeostasis 14 1.04E-4 0.08
15 SGTC_476 mead acid 3.66 10.0 µM 1.47E-5 2 1.27E-4 0.37
16 SGTC_2627 acetyl isogambogic acid 3.44 100.0 µM 4.18E-5 TSC3-RPN4 12 2.87E-4 0.07
17 SGTC_2043 5101342 3.37 149.0 µM 5.97E-5 SWF1 & branched chain AA biosynthesis 8 3.79E-4 0.02
18 SGTC_2596 sinensetin 3.30 100.0 µM 8.09E-5 14 4.80E-4 0.12
19 SGTC_1529 apigenin 3.29 74.0 µM 8.57E-5 10 5.02E-4 0.10
20 SGTC_1262 0737-0114 3.23 49.6 µM 1.14E-4 10 6.24E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.272 7.36E-58 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.216 1.21E-36 YOR074C CDC21 het Thymidylate synthase, required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S
0.182 2.51E-26 YGR267C FOL2 het GTP-cyclohydrolase I, catalyzes the first step in the folic acid biosynthetic pathway
0.156 1.14E-19 YNR003C RPC34 het RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
0.155 1.63E-19 YER056C FCY2 hom Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress
0.130 4.91E-14 YDL145C COP1 het Alpha subunit of COPI vesicle coatomer complex, which surrounds transport vesicles in the early secretory pathway
0.128 1.11E-13 YOR073W SGO1 hom Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability
0.120 2.60E-12 YOR062C_p YOR062C_p hom Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
0.120 2.97E-12 YLR315W NKP2 hom Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
0.114 3.69E-11 YPL010W RET3 het Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.114 3.75E-11 YER147C SCC4 het Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
0.112 8.76E-11 YPL195W APL5 hom Delta adaptin-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway, suppressor of loss of casein kinase 1 function
0.109 2.65E-10 YGR154C GTO1 hom Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization
0.108 3.75E-10 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.106 6.76E-10 YBR288C APM3 hom Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway