YNR030W / ALG12

Alpha-1,6-mannosyltransferase localized to the ER; responsible for the addition of the alpha-1,6 mannose to dolichol-linked Man7GlcNAc2, acts in the dolichol pathway for N-glycosylation

Zygosity: Homozygous strain
fixedexpanded
Profile for YNR030W / ALG12

Click on Significant Values for Screen Details ID:SGTC_41|Compound:0478-0632|FD-Score:3.43|P-value:7.30E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:4.67|P-value:6.71E-6 ID:SGTC_56|Compound:0957-0365|FD-Score:-3.32|P-value:7.04E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-11.30|P-value:4.00E-27 ID:SGTC_62|Compound:0180-0423|FD-Score:-4.34|P-value:1.59E-5 ID:SGTC_444|Compound:curcumin|FD-Score:-3.78|P-value:1.44E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:6.17|P-value:4.04E-9 ID:SGTC_663|Compound:1486-0733|FD-Score:4.53|P-value:1.22E-5 ID:SGTC_676|Compound:falcarinol|FD-Score:4.29|P-value:3.25E-5 ID:SGTC_710|Compound:3448-4187|FD-Score:-3.74|P-value:1.63E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:4.72|P-value:5.39E-6 ID:SGTC_792|Compound:4534-1957|FD-Score:-3.22|P-value:9.85E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:7.18|P-value:8.66E-12 ID:SGTC_873|Compound:0591-4978|FD-Score:3.67|P-value:3.25E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:5.35|P-value:2.91E-7 ID:SGTC_878|Compound:0828-0289|FD-Score:3.47|P-value:6.45E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.33|P-value:6.88E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.22|P-value:9.86E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.95|P-value:1.22E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:-3.28|P-value:8.07E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.79|P-value:2.18E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.31|P-value:7.37E-4 ID:SGTC_1526|Compound:st051518|FD-Score:4.79|P-value:4.01E-6 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.37|P-value:8.85E-4 ID:SGTC_1651|Compound:st011928|FD-Score:3.83|P-value:1.83E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.54|P-value:5.02E-4 ID:SGTC_1677|Compound:st019204|FD-Score:4.17|P-value:5.28E-5 ID:SGTC_1702|Compound:st027038|FD-Score:4.54|P-value:1.19E-5 ID:SGTC_1748|Compound:st037455|FD-Score:5.03|P-value:1.37E-6 ID:SGTC_1841|Compound:st054148|FD-Score:3.74|P-value:2.61E-4 ID:SGTC_1942|Compound:st076330|FD-Score:7.95|P-value:4.66E-14 ID:SGTC_1955|Compound:wogonin|FD-Score:6.22|P-value:3.06E-9 ID:SGTC_2007|Compound:4011539|FD-Score:3.81|P-value:2.00E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.43|P-value:1.87E-5 ID:SGTC_2413|Compound:inermin|FD-Score:7.90|P-value:6.93E-14 ID:SGTC_2436|Compound:5633522|FD-Score:3.67|P-value:3.33E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.93|P-value:1.28E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:4.01|P-value:9.75E-5 ID:SGTC_2657|Compound:nystatin|FD-Score:4.00|P-value:9.96E-5 ID:SGTC_2699|Compound:st077704|FD-Score:-3.45|P-value:4.53E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.54|P-value:5.19E-4 ID:SGTC_2950|Compound:9072895|FD-Score:5.39|P-value:2.39E-7 ID:SGTC_3049|Compound:9092223|FD-Score:-4.69|P-value:3.68E-6 ID:SGTC_3145|Compound:9085516|FD-Score:5.65|P-value:6.40E-8 ID:SGTC_3154|Compound:9100113|FD-Score:3.39|P-value:8.27E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-3.30|P-value:7.52E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.39|P-value:8.48E-4 ID:SGTC_3260|Compound:9137109|FD-Score:-3.90|P-value:9.27E-5 ID:SGTC_3277|Compound:9138724|FD-Score:-3.93|P-value:8.17E-5 ID:SGTC_3299|Compound:9121982|FD-Score:3.39|P-value:8.41E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:3.43|P-value:7.30E-4 ID:SGTC_54|Compound:0159-0055|FD-Score:4.67|P-value:6.71E-6 ID:SGTC_56|Compound:0957-0365|FD-Score:-3.32|P-value:7.04E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-11.30|P-value:4.00E-27 ID:SGTC_62|Compound:0180-0423|FD-Score:-4.34|P-value:1.59E-5 ID:SGTC_444|Compound:curcumin|FD-Score:-3.78|P-value:1.44E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:6.17|P-value:4.04E-9 ID:SGTC_663|Compound:1486-0733|FD-Score:4.53|P-value:1.22E-5 ID:SGTC_676|Compound:falcarinol|FD-Score:4.29|P-value:3.25E-5 ID:SGTC_710|Compound:3448-4187|FD-Score:-3.74|P-value:1.63E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:4.72|P-value:5.39E-6 ID:SGTC_792|Compound:4534-1957|FD-Score:-3.22|P-value:9.85E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:7.18|P-value:8.66E-12 ID:SGTC_873|Compound:0591-4978|FD-Score:3.67|P-value:3.25E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:5.35|P-value:2.91E-7 ID:SGTC_878|Compound:0828-0289|FD-Score:3.47|P-value:6.45E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.33|P-value:6.88E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.22|P-value:9.86E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.95|P-value:1.22E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:-3.28|P-value:8.07E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.79|P-value:2.18E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.31|P-value:7.37E-4 ID:SGTC_1526|Compound:st051518|FD-Score:4.79|P-value:4.01E-6 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.37|P-value:8.85E-4 ID:SGTC_1651|Compound:st011928|FD-Score:3.83|P-value:1.83E-4 ID:SGTC_1672|Compound:st016598|FD-Score:3.54|P-value:5.02E-4 ID:SGTC_1677|Compound:st019204|FD-Score:4.17|P-value:5.28E-5 ID:SGTC_1702|Compound:st027038|FD-Score:4.54|P-value:1.19E-5 ID:SGTC_1748|Compound:st037455|FD-Score:5.03|P-value:1.37E-6 ID:SGTC_1841|Compound:st054148|FD-Score:3.74|P-value:2.61E-4 ID:SGTC_1942|Compound:st076330|FD-Score:7.95|P-value:4.66E-14 ID:SGTC_1955|Compound:wogonin|FD-Score:6.22|P-value:3.06E-9 ID:SGTC_2007|Compound:4011539|FD-Score:3.81|P-value:2.00E-4 ID:SGTC_2399|Compound:5884133|FD-Score:4.43|P-value:1.87E-5 ID:SGTC_2413|Compound:inermin|FD-Score:7.90|P-value:6.93E-14 ID:SGTC_2436|Compound:5633522|FD-Score:3.67|P-value:3.33E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:3.93|P-value:1.28E-4 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:4.01|P-value:9.75E-5 ID:SGTC_2657|Compound:nystatin|FD-Score:4.00|P-value:9.96E-5 ID:SGTC_2699|Compound:st077704|FD-Score:-3.45|P-value:4.53E-4 ID:SGTC_2943|Compound:9068529|FD-Score:3.54|P-value:5.19E-4 ID:SGTC_2950|Compound:9072895|FD-Score:5.39|P-value:2.39E-7 ID:SGTC_3049|Compound:9092223|FD-Score:-4.69|P-value:3.68E-6 ID:SGTC_3145|Compound:9085516|FD-Score:5.65|P-value:6.40E-8 ID:SGTC_3154|Compound:9100113|FD-Score:3.39|P-value:8.27E-4 ID:SGTC_3173|Compound:9105693|FD-Score:-3.30|P-value:7.52E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.39|P-value:8.48E-4 ID:SGTC_3260|Compound:9137109|FD-Score:-3.90|P-value:9.27E-5 ID:SGTC_3277|Compound:9138724|FD-Score:-3.93|P-value:8.17E-5 ID:SGTC_3299|Compound:9121982|FD-Score:3.39|P-value:8.41E-4

Top fitness defect scores for YNR030W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1942 st076330 7.95 65.2 µM 4.66E-14 1 9.27E-16
2 SGTC_2413 inermin 7.90 26.1 µM 6.93E-14 7 1.45E-15
3 SGTC_842 0271-0008 7.18 11.1 µM 8.66E-12 heme biosynthesis & mitochondrial translocase 4 3.36E-13
4 SGTC_1955 wogonin 6.22 32.4 µM 3.06E-9 3 2.52E-10
5 SGTC_513 4245-1575 6.17 41.7 µM 4.04E-9 unfolded protein response 44 3.45E-10
6 SGTC_3145 9085516 5.65 49.5 µM 6.40E-8 fatty acid desaturase (OLE1) 8 7.81E-9
7 SGTC_2950 9072895 5.39 21.5 µM 2.39E-7 12 3.45E-8
8 SGTC_875 0709-0210 5.35 25.7 µM 2.91E-7 fatty acid desaturase (OLE1) 14 4.32E-8
9 SGTC_1748 st037455 5.03 53.6 µM 1.37E-6 8 2.48E-7
10 SGTC_1526 st051518 4.79 75.0 µM 4.01E-6 9 8.37E-7
11 SGTC_774 k060-0086 4.72 136.0 µM 5.39E-6 9 1.17E-6
12 SGTC_54 0159-0055 4.67 9.0 µM 6.71E-6 ERG2 32 1.50E-6
13 SGTC_1702 st027038 4.54 51.0 µM 1.19E-5 5 2.86E-6
14 SGTC_663 1486-0733 4.53 13.9 µM 1.22E-5 cell wall signaling 38 2.93E-6
15 SGTC_2399 5884133 4.43 200.0 µM 1.87E-5 cell wall signaling 55 4.76E-6
16 SGTC_676 falcarinol 4.29 140.0 µM 3.25E-5 46 8.89E-6
17 SGTC_1677 st019204 4.17 53.9 µM 5.28E-5 13 1.54E-5
18 SGTC_2613 3',4'-dimethoxydalbergione 4.01 24.6 µM 9.75E-5 23 3.08E-5
19 SGTC_2657 nystatin 4.00 74.4 µM 9.96E-5 8 3.16E-5
20 SGTC_976 1348-1067 3.95 47.6 µM 1.22E-4 60S ribosome export 31 3.96E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.174 4.21E-24 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.171 1.85E-23 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.164 1.27E-21 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.155 1.73E-19 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.123 8.40E-13 YJR075W HOC1 hom Alpha-1,6-mannosyltransferase involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
0.121 2.17E-12 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.118 7.61E-12 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.117 1.11E-11 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.113 4.81E-11 YOL146W PSF3 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.110 1.53E-10 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.109 3.12E-10 YJL183W MNN11 hom Subunit of a Golgi mannosyltransferase complex that also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
0.108 3.26E-10 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.107 5.75E-10 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response
0.105 1.10E-9 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.101 5.28E-9 YNL323W LEM3 hom Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane