YOL054W / PSH1

E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p

Zygosity: Homozygous strain
fixedexpanded
Profile for YOL054W / PSH1

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:3.23|P-value:9.68E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-8.71|P-value:3.61E-17 ID:SGTC_52|Compound:0329-0193|FD-Score:3.38|P-value:5.91E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.18|P-value:1.59E-9 ID:SGTC_149|Compound:3970-0822|FD-Score:3.74|P-value:1.69E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.76|P-value:1.55E-4 ID:SGTC_191|Compound:3914-0123|FD-Score:-3.25|P-value:9.28E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.84|P-value:1.17E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:4.92|P-value:1.19E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:4.90|P-value:1.29E-6 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.83|P-value:1.22E-4 ID:SGTC_462|Compound:ag-879|FD-Score:3.30|P-value:7.72E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:6.39|P-value:4.45E-10 ID:SGTC_547|Compound:1120-0019|FD-Score:4.52|P-value:7.18E-6 ID:SGTC_558|Compound:k064-0027|FD-Score:3.61|P-value:2.66E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.39|P-value:5.65E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.67|P-value:3.75E-6 ID:SGTC_1101|Compound:ebselen|FD-Score:5.15|P-value:3.87E-7 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.36|P-value:6.39E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-3.27|P-value:8.73E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.76|P-value:1.55E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.78|P-value:1.42E-4 ID:SGTC_1387|Compound:3342-1166|FD-Score:5.09|P-value:5.38E-7 ID:SGTC_1652|Compound:st011932|FD-Score:4.60|P-value:5.25E-6 ID:SGTC_1707|Compound:st032323|FD-Score:4.72|P-value:2.96E-6 ID:SGTC_1818|Compound:st052136|FD-Score:-3.52|P-value:3.77E-4 ID:SGTC_1825|Compound:st052143|FD-Score:3.85|P-value:1.12E-4 ID:SGTC_1942|Compound:st076330|FD-Score:3.68|P-value:2.05E-4 ID:SGTC_2027|Compound:5150920|FD-Score:3.51|P-value:3.84E-4 ID:SGTC_2102|Compound:5377816|FD-Score:3.82|P-value:1.25E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.34|P-value:6.87E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-3.35|P-value:6.65E-4 ID:SGTC_2366|Compound:9070441|FD-Score:-4.06|P-value:5.13E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-5.02|P-value:7.50E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.40|P-value:5.56E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.27|P-value:8.60E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:9.12|P-value:1.11E-18 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:4.38|P-value:1.34E-5 ID:SGTC_2624|Compound:nerol|FD-Score:-3.48|P-value:4.27E-4 ID:SGTC_2699|Compound:st077704|FD-Score:-3.32|P-value:7.42E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:3.66|P-value:2.20E-4 ID:SGTC_2783|Compound:7738165|FD-Score:3.60|P-value:2.74E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.56|P-value:3.17E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.88|P-value:9.98E-5 ID:SGTC_3002|Compound:9075603|FD-Score:4.17|P-value:3.24E-5 ID:SGTC_3003|Compound:9075612|FD-Score:3.25|P-value:9.27E-4 ID:SGTC_3133|Compound:9127736|FD-Score:3.65|P-value:2.29E-4 ID:SGTC_3164|Compound:9100705|FD-Score:5.52|P-value:6.12E-8 ID:SGTC_3186|Compound:9110464|FD-Score:5.02|P-value:7.49E-7 ID:SGTC_3218|Compound:9131285|FD-Score:3.85|P-value:1.11E-4 ID:SGTC_3311|Compound:9131146|FD-Score:4.39|P-value:1.25E-5 ID:SGTC_39|Compound:0986-0283|FD-Score:3.23|P-value:9.68E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-8.71|P-value:3.61E-17 ID:SGTC_52|Compound:0329-0193|FD-Score:3.38|P-value:5.91E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.18|P-value:1.59E-9 ID:SGTC_149|Compound:3970-0822|FD-Score:3.74|P-value:1.69E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.76|P-value:1.55E-4 ID:SGTC_191|Compound:3914-0123|FD-Score:-3.25|P-value:9.28E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.84|P-value:1.17E-4 ID:SGTC_214|Compound:4373-3496|FD-Score:4.92|P-value:1.19E-6 ID:SGTC_312|Compound:4092-0653|FD-Score:4.90|P-value:1.29E-6 ID:SGTC_385|Compound:1494-0393|FD-Score:-3.83|P-value:1.22E-4 ID:SGTC_462|Compound:ag-879|FD-Score:3.30|P-value:7.72E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:6.39|P-value:4.45E-10 ID:SGTC_547|Compound:1120-0019|FD-Score:4.52|P-value:7.18E-6 ID:SGTC_558|Compound:k064-0027|FD-Score:3.61|P-value:2.66E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.39|P-value:5.65E-4 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.67|P-value:3.75E-6 ID:SGTC_1101|Compound:ebselen|FD-Score:5.15|P-value:3.87E-7 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:3.36|P-value:6.39E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-3.27|P-value:8.73E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.76|P-value:1.55E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.78|P-value:1.42E-4 ID:SGTC_1387|Compound:3342-1166|FD-Score:5.09|P-value:5.38E-7 ID:SGTC_1652|Compound:st011932|FD-Score:4.60|P-value:5.25E-6 ID:SGTC_1707|Compound:st032323|FD-Score:4.72|P-value:2.96E-6 ID:SGTC_1818|Compound:st052136|FD-Score:-3.52|P-value:3.77E-4 ID:SGTC_1825|Compound:st052143|FD-Score:3.85|P-value:1.12E-4 ID:SGTC_1942|Compound:st076330|FD-Score:3.68|P-value:2.05E-4 ID:SGTC_2027|Compound:5150920|FD-Score:3.51|P-value:3.84E-4 ID:SGTC_2102|Compound:5377816|FD-Score:3.82|P-value:1.25E-4 ID:SGTC_2187|Compound:6125751|FD-Score:3.34|P-value:6.87E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-3.35|P-value:6.65E-4 ID:SGTC_2366|Compound:9070441|FD-Score:-4.06|P-value:5.13E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-5.02|P-value:7.50E-7 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.40|P-value:5.56E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.27|P-value:8.60E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:9.12|P-value:1.11E-18 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:4.38|P-value:1.34E-5 ID:SGTC_2624|Compound:nerol|FD-Score:-3.48|P-value:4.27E-4 ID:SGTC_2699|Compound:st077704|FD-Score:-3.32|P-value:7.42E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:3.66|P-value:2.20E-4 ID:SGTC_2783|Compound:7738165|FD-Score:3.60|P-value:2.74E-4 ID:SGTC_2801|Compound:7907800|FD-Score:3.56|P-value:3.17E-4 ID:SGTC_2808|Compound:7946468|FD-Score:3.88|P-value:9.98E-5 ID:SGTC_3002|Compound:9075603|FD-Score:4.17|P-value:3.24E-5 ID:SGTC_3003|Compound:9075612|FD-Score:3.25|P-value:9.27E-4 ID:SGTC_3133|Compound:9127736|FD-Score:3.65|P-value:2.29E-4 ID:SGTC_3164|Compound:9100705|FD-Score:5.52|P-value:6.12E-8 ID:SGTC_3186|Compound:9110464|FD-Score:5.02|P-value:7.49E-7 ID:SGTC_3218|Compound:9131285|FD-Score:3.85|P-value:1.11E-4 ID:SGTC_3311|Compound:9131146|FD-Score:4.39|P-value:1.25E-5

Top fitness defect scores for YOL054W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2619 obliquin 9.12 87.9 µM 1.11E-18 cell wall 31 3.70E-20
2 SGTC_514 4466-0038 6.39 2.6 nM 4.45E-10 cell wall 65 8.22E-11
3 SGTC_58 0957-0393 6.18 9.0 µM 1.59E-9 cell wall 205 3.27E-10
4 SGTC_3164 9100705 5.52 49.5 µM 6.12E-8 1 1.73E-8
5 SGTC_1101 ebselen 5.15 5.3 µM 3.87E-7 8 1.28E-7
6 SGTC_1387 3342-1166 5.09 1.8 µM 5.38E-7 mitochondrial processes 4 1.82E-7
7 SGTC_3186 9110464 5.02 49.5 µM 7.49E-7 RPP1 & pyrimidine depletion 3 2.62E-7
8 SGTC_214 4373-3496 4.92 70.0 µM 1.19E-6 32 4.31E-7
9 SGTC_312 4092-0653 4.90 6.8 µM 1.29E-6 ergosterol depletion effects on membrane 59 4.73E-7
10 SGTC_1707 st032323 4.72 6.3 µM 2.96E-6 DNA damage response 38 1.16E-6
11 SGTC_1000 4048-0884 4.67 8.7 µM 3.75E-6 copper-dependent oxidative stress 20 1.50E-6
12 SGTC_1652 st011932 4.60 78.0 µM 5.25E-6 RSC complex & mRNA processing 7 2.16E-6
13 SGTC_547 1120-0019 4.52 610.0 nM 7.18E-6 azole & statin 22 3.04E-6
14 SGTC_3311 9131146 4.39 2.9 µM 1.25E-5 cell wall 105 5.54E-6
15 SGTC_2621 lithochol-11-enic acid 4.38 100.0 µM 1.34E-5 15 5.95E-6
16 SGTC_3002 9075603 4.17 71.4 µM 3.24E-5 17 1.55E-5
17 SGTC_2808 7946468 3.88 45.5 µM 9.98E-5 24 5.26E-5
18 SGTC_3218 9131285 3.85 49.5 µM 1.11E-4 endomembrane recycling 49 5.92E-5
19 SGTC_1825 st052143 3.85 62.6 µM 1.12E-4 15 5.96E-5
20 SGTC_212 3807-4682 3.84 38.8 µM 1.17E-4 tubulin folding & SWR complex 74 6.23E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.349 4.80E-97 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.348 6.86E-96 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.300 1.60E-70 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.292 6.25E-67 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.273 3.02E-58 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.247 1.07E-47 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.241 1.66E-45 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.237 5.43E-44 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.234 3.60E-43 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.197 1.04E-30 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.196 1.97E-30 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.196 2.80E-30 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.193 1.61E-29 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.188 4.68E-28 YDR001C NTH1 hom Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication
0.180 9.41E-26 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication