YOR086C / TCB1

Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact

Zygosity: Homozygous strain
fixedexpanded
Profile for YOR086C / TCB1

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.05|P-value:4.95E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:3.32|P-value:1.75E-4 ID:SGTC_228|Compound:caffeine|FD-Score:3.49|P-value:8.39E-5 ID:SGTC_458|Compound:nppb|FD-Score:3.33|P-value:1.63E-4 ID:SGTC_468|Compound:gf-109203x|FD-Score:3.01|P-value:5.85E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.38|P-value:1.36E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:15.50|P-value:3.85E-62 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.25|P-value:2.30E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:-3.67|P-value:5.67E-5 ID:SGTC_798|Compound:1287-0182|FD-Score:3.44|P-value:1.05E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:4.32|P-value:1.74E-6 ID:SGTC_816|Compound:0325-0417|FD-Score:4.01|P-value:8.17E-6 ID:SGTC_823|Compound:0417-1665|FD-Score:3.14|P-value:3.52E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:-3.07|P-value:6.39E-4 ID:SGTC_901|Compound:1112-0062|FD-Score:-3.18|P-value:4.18E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.22|P-value:2.88E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:4.23|P-value:2.72E-6 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.01|P-value:5.75E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.02|P-value:7.59E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-4.16|P-value:5.84E-6 ID:SGTC_1010|Compound:4112-3296|FD-Score:2.94|P-value:7.55E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.55|P-value:1.34E-9 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.58|P-value:5.76E-5 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.99|P-value:8.79E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-2.96|P-value:9.50E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.50|P-value:6.55E-7 ID:SGTC_1627|Compound:st003713|FD-Score:3.96|P-value:1.04E-5 ID:SGTC_1636|Compound:st007707|FD-Score:2.88|P-value:9.31E-4 ID:SGTC_1654|Compound:st012436|FD-Score:3.21|P-value:2.65E-4 ID:SGTC_1693|Compound:xanthone|FD-Score:-3.13|P-value:5.02E-4 ID:SGTC_1712|Compound:st032288|FD-Score:10.00|P-value:4.77E-27 ID:SGTC_1723|Compound:st034307|FD-Score:-3.33|P-value:2.30E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.69|P-value:3.47E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-3.30|P-value:2.58E-4 ID:SGTC_2021|Compound:4019055|FD-Score:7.16|P-value:8.71E-15 ID:SGTC_2178|Compound:5812956|FD-Score:-3.36|P-value:2.01E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:2.91|P-value:8.47E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.36|P-value:1.49E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.43|P-value:1.10E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.52|P-value:7.35E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:2.90|P-value:8.83E-4 ID:SGTC_2708|Compound:st078559|FD-Score:2.92|P-value:8.09E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.51|P-value:7.74E-5 ID:SGTC_3227|Compound:9130768|FD-Score:-3.67|P-value:5.54E-5 ID:SGTC_3239|Compound:9133757|FD-Score:-4.47|P-value:1.24E-6 ID:SGTC_3241|Compound:9133421|FD-Score:-3.37|P-value:1.94E-4 ID:SGTC_3247|Compound:9135149|FD-Score:-3.10|P-value:5.64E-4 ID:SGTC_3256|Compound:9138902|FD-Score:-3.38|P-value:1.90E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-3.62|P-value:7.05E-5 ID:SGTC_3|Compound:0833-0587|FD-Score:3.05|P-value:4.95E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:3.32|P-value:1.75E-4 ID:SGTC_228|Compound:caffeine|FD-Score:3.49|P-value:8.39E-5 ID:SGTC_458|Compound:nppb|FD-Score:3.33|P-value:1.63E-4 ID:SGTC_468|Compound:gf-109203x|FD-Score:3.01|P-value:5.85E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.38|P-value:1.36E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:15.50|P-value:3.85E-62 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:3.25|P-value:2.30E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:-3.67|P-value:5.67E-5 ID:SGTC_798|Compound:1287-0182|FD-Score:3.44|P-value:1.05E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:4.32|P-value:1.74E-6 ID:SGTC_816|Compound:0325-0417|FD-Score:4.01|P-value:8.17E-6 ID:SGTC_823|Compound:0417-1665|FD-Score:3.14|P-value:3.52E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:-3.07|P-value:6.39E-4 ID:SGTC_901|Compound:1112-0062|FD-Score:-3.18|P-value:4.18E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.22|P-value:2.88E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:4.23|P-value:2.72E-6 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.01|P-value:5.75E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.02|P-value:7.59E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-4.16|P-value:5.84E-6 ID:SGTC_1010|Compound:4112-3296|FD-Score:2.94|P-value:7.55E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.55|P-value:1.34E-9 ID:SGTC_1246|Compound:0422-0085|FD-Score:3.58|P-value:5.76E-5 ID:SGTC_1251|Compound:0490-5699|FD-Score:3.99|P-value:8.79E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-2.96|P-value:9.50E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.50|P-value:6.55E-7 ID:SGTC_1627|Compound:st003713|FD-Score:3.96|P-value:1.04E-5 ID:SGTC_1636|Compound:st007707|FD-Score:2.88|P-value:9.31E-4 ID:SGTC_1654|Compound:st012436|FD-Score:3.21|P-value:2.65E-4 ID:SGTC_1693|Compound:xanthone|FD-Score:-3.13|P-value:5.02E-4 ID:SGTC_1712|Compound:st032288|FD-Score:10.00|P-value:4.77E-27 ID:SGTC_1723|Compound:st034307|FD-Score:-3.33|P-value:2.30E-4 ID:SGTC_1936|Compound:st074407|FD-Score:3.69|P-value:3.47E-5 ID:SGTC_2016|Compound:5121758|FD-Score:-3.30|P-value:2.58E-4 ID:SGTC_2021|Compound:4019055|FD-Score:7.16|P-value:8.71E-15 ID:SGTC_2178|Compound:5812956|FD-Score:-3.36|P-value:2.01E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:2.91|P-value:8.47E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.36|P-value:1.49E-4 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.43|P-value:1.10E-4 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.52|P-value:7.35E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:2.90|P-value:8.83E-4 ID:SGTC_2708|Compound:st078559|FD-Score:2.92|P-value:8.09E-4 ID:SGTC_3205|Compound:9113483|FD-Score:3.51|P-value:7.74E-5 ID:SGTC_3227|Compound:9130768|FD-Score:-3.67|P-value:5.54E-5 ID:SGTC_3239|Compound:9133757|FD-Score:-4.47|P-value:1.24E-6 ID:SGTC_3241|Compound:9133421|FD-Score:-3.37|P-value:1.94E-4 ID:SGTC_3247|Compound:9135149|FD-Score:-3.10|P-value:5.64E-4 ID:SGTC_3256|Compound:9138902|FD-Score:-3.38|P-value:1.90E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-3.62|P-value:7.05E-5

Top fitness defect scores for YOR086C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_528 3492-0019 15.50 172.0 µM 3.85E-62 calcium & mitochondrial duress 12 8.70E-55
2 SGTC_1712 st032288 10.00 53.4 µM 4.77E-27 calcium & mitochondrial duress 24 6.61E-24
3 SGTC_2021 4019055 7.16 173.0 µM 8.71E-15 endomembrane recycling 29 4.01E-13
4 SGTC_1083 didemethylchlorpromazine sulfoxide 5.55 707.4 nM 1.34E-9 redox potentiating 34 1.45E-8
5 SGTC_1597 7-benzyloxygramine 4.50 47.5 µM 6.55E-7 calcium & mitochondrial duress 88 3.35E-6
6 SGTC_813 k007-0158 4.32 173.0 µM 1.74E-6 fatty acid desaturase (OLE1) 34 7.93E-6
7 SGTC_996 3970-0799 4.23 1.8 µM 2.72E-6 74 1.17E-5
8 SGTC_994 3910-0338 4.22 138.0 µM 2.88E-6 calcium & mitochondrial duress 40 1.23E-5
9 SGTC_816 0325-0417 4.01 81.3 µM 8.17E-6 12 3.08E-5
10 SGTC_1251 0490-5699 3.99 265.0 µM 8.79E-6 31 3.29E-5
11 SGTC_1627 st003713 3.96 25.6 µM 1.04E-5 calcium & mitochondrial duress 77 3.81E-5
12 SGTC_1936 st074407 3.69 74.3 µM 3.47E-5 DNA damage response 44 1.10E-4
13 SGTC_1246 0422-0085 3.58 105.0 µM 5.76E-5 48 1.72E-4
14 SGTC_2589 lobaric acid 3.52 100.0 µM 7.35E-5 35 2.13E-4
15 SGTC_3205 9113483 3.51 49.5 µM 7.74E-5 47 2.22E-4
16 SGTC_228 caffeine 3.49 993.1 µM 8.39E-5 16 2.39E-4
17 SGTC_798 1287-0182 3.44 476.0 µM 1.05E-4 37 2.91E-4
18 SGTC_2588 indole-3-carbinol 3.43 100.0 µM 1.10E-4 70 3.04E-4
19 SGTC_526 4469-0568 3.38 170.0 µM 1.36E-4 azole & statin 54 3.64E-4
20 SGTC_2288 7946289 3.36 167.3 µM 1.49E-4 53 3.96E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.575 4.30E-294 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.399 1.16E-128 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.358 6.82E-102 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.338 1.23E-90 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.320 1.60E-80 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.293 1.19E-67 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.285 6.42E-64 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.268 3.34E-56 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.264 1.74E-54 YLR404W FLD1 hom Seipin protein involved in lipid droplet morphology, number, and size; proposed to be involved in lipid metabolism; related to the human BSCL2 which is associated with lipodystrophy
0.262 6.26E-54 YDR093W DNF2 hom Aminophospholipid translocase (flippase) that localizes primarily to the plasma membrane; contributes to endocytosis, protein transport and cell polarity; type 4 P-type ATPase
0.259 2.09E-52 YJL129C TRK1 hom Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
0.257 7.08E-52 YNL264C PDR17 hom Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition
0.256 1.69E-51 YGL035C MIG1 hom Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion
0.256 2.58E-51 YOL016C CMK2 hom Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
0.245 3.59E-47 YGR237C_p YGR237C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm