YOR249C / APC5

Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YOR249C / APC5

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:3.59|P-value:4.21E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:-5.31|P-value:1.68E-7 ID:SGTC_166|Compound:k072-0058|FD-Score:3.58|P-value:4.39E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-5.28|P-value:1.99E-7 ID:SGTC_756|Compound:0508-1999|FD-Score:4.78|P-value:3.94E-6 ID:SGTC_761|Compound:k216-0224|FD-Score:3.78|P-value:2.17E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:-3.19|P-value:9.84E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.39|P-value:8.25E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:-3.32|P-value:6.48E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.86|P-value:9.62E-5 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.50|P-value:5.77E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:-4.64|P-value:4.00E-6 ID:SGTC_1571|Compound:st070080|FD-Score:-3.61|P-value:2.42E-4 ID:SGTC_1849|Compound:st056239|FD-Score:4.07|P-value:7.50E-5 ID:SGTC_1967|Compound:st070177|FD-Score:-3.40|P-value:4.94E-4 ID:SGTC_2016|Compound:5121758|FD-Score:7.74|P-value:1.81E-13 ID:SGTC_2182|Compound:6325192|FD-Score:-3.51|P-value:3.42E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.39|P-value:5.18E-4 ID:SGTC_2294|Compound:7971431|FD-Score:3.36|P-value:9.24E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.46|P-value:1.61E-5 ID:SGTC_2321|Compound:7390090|FD-Score:6.36|P-value:1.25E-9 ID:SGTC_2326|Compound:6164455|FD-Score:4.21|P-value:4.34E-5 ID:SGTC_2340|Compound:7987427|FD-Score:-4.15|P-value:3.17E-5 ID:SGTC_2342|Compound:7990637|FD-Score:-3.21|P-value:9.25E-4 ID:SGTC_2400|Compound:5203494|FD-Score:6.32|P-value:1.54E-9 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.46|P-value:4.06E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-4.92|P-value:1.14E-6 ID:SGTC_3107|Compound:9121808|FD-Score:-4.04|P-value:4.95E-5 ID:SGTC_3268|Compound:9137683|FD-Score:3.64|P-value:3.62E-4 ID:SGTC_3285|Compound:9112818|FD-Score:5.16|P-value:7.00E-7 ID:SGTC_3311|Compound:9131146|FD-Score:-3.48|P-value:3.84E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.59|P-value:4.21E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:-5.31|P-value:1.68E-7 ID:SGTC_166|Compound:k072-0058|FD-Score:3.58|P-value:4.39E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-5.28|P-value:1.99E-7 ID:SGTC_756|Compound:0508-1999|FD-Score:4.78|P-value:3.94E-6 ID:SGTC_761|Compound:k216-0224|FD-Score:3.78|P-value:2.17E-4 ID:SGTC_967|Compound:1306-0166|FD-Score:-3.19|P-value:9.84E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.39|P-value:8.25E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:-3.32|P-value:6.48E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.86|P-value:9.62E-5 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.50|P-value:5.77E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:-4.64|P-value:4.00E-6 ID:SGTC_1571|Compound:st070080|FD-Score:-3.61|P-value:2.42E-4 ID:SGTC_1849|Compound:st056239|FD-Score:4.07|P-value:7.50E-5 ID:SGTC_1967|Compound:st070177|FD-Score:-3.40|P-value:4.94E-4 ID:SGTC_2016|Compound:5121758|FD-Score:7.74|P-value:1.81E-13 ID:SGTC_2182|Compound:6325192|FD-Score:-3.51|P-value:3.42E-4 ID:SGTC_2288|Compound:7946289|FD-Score:-3.39|P-value:5.18E-4 ID:SGTC_2294|Compound:7971431|FD-Score:3.36|P-value:9.24E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.46|P-value:1.61E-5 ID:SGTC_2321|Compound:7390090|FD-Score:6.36|P-value:1.25E-9 ID:SGTC_2326|Compound:6164455|FD-Score:4.21|P-value:4.34E-5 ID:SGTC_2340|Compound:7987427|FD-Score:-4.15|P-value:3.17E-5 ID:SGTC_2342|Compound:7990637|FD-Score:-3.21|P-value:9.25E-4 ID:SGTC_2400|Compound:5203494|FD-Score:6.32|P-value:1.54E-9 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.46|P-value:4.06E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-4.92|P-value:1.14E-6 ID:SGTC_3107|Compound:9121808|FD-Score:-4.04|P-value:4.95E-5 ID:SGTC_3268|Compound:9137683|FD-Score:3.64|P-value:3.62E-4 ID:SGTC_3285|Compound:9112818|FD-Score:5.16|P-value:7.00E-7 ID:SGTC_3311|Compound:9131146|FD-Score:-3.48|P-value:3.84E-4

Top fitness defect scores for YOR249C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2016 5121758 7.74 140.0 µM 1.81E-13 redox potentiating 2 5.01E-15 1.03
2 SGTC_2321 7390090 6.36 138.8 µM 1.25E-9 1 1.01E-10 1.61
3 SGTC_2400 5203494 6.32 178.7 µM 1.54E-9 RSC complex & mRNA processing 7 1.28E-10 0.08
4 SGTC_3285 9112818 5.16 20.9 µM 7.00E-7 cell wall 7 1.23E-7 0.39
5 SGTC_756 0508-1999 4.78 28.3 µM 3.94E-6 RPP1 & pyrimidine depletion 5 8.57E-7 0.41
6 SGTC_2316 5844804 4.46 197.4 µM 1.61E-5 10 4.16E-6 0.64
7 SGTC_2326 6164455 4.21 177.6 µM 4.34E-5 mitochondrial processes 11 1.27E-5 0.13
8 SGTC_1849 st056239 4.07 19.2 µM 7.50E-5 RPP1 & pyrimidine depletion 2 2.35E-5 0.09
9 SGTC_761 k216-0224 3.78 480.0 µM 2.17E-4 5 7.77E-5 0.01
10 SGTC_3268 9137683 3.64 49.5 µM 3.62E-4 6 1.38E-4 0.23
11 SGTC_43 0570-0105 3.59 72.5 µM 4.21E-4 ERG2 11 1.64E-4 0.02
12 SGTC_166 k072-0058 3.58 166.4 µM 4.39E-4 DNA intercalators 8 1.72E-4 0.04
13 SGTC_1391 3442-0225 3.50 151.0 µM 5.77E-4 5 2.34E-4 0.05
14 SGTC_969 1313-0238 3.39 134.0 µM 8.25E-4 mitochondrial processes 28 3.49E-4 0.02
15 SGTC_2294 7971431 3.36 200.0 µM 9.24E-4 7 3.97E-4 0.15
16 SGTC_226 1,3-diallylurea 3.33 8.3 mM 0.00101 16 4.38E-4 0.08
17 SGTC_2881 9058953 3.31 52.0 µM 0.00108 14 4.70E-4 0.02
18 SGTC_3286 9117135 3.30 37.2 µM 0.00111 17 4.89E-4 0.11
19 SGTC_2114 5491910 3.22 81.2 µM 0.00142 11 6.42E-4 0.19
20 SGTC_782 0083-0095 3.21 30.5 µM 0.00145 5 6.57E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.112 6.91E-11 YBR084W MIS1 hom Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase
0.108 3.57E-10 YDR168W CDC37 het Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
0.101 4.80E-9 YKR068C BET3 het Hydrophilic protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles; component of the TRAPP (transport protein particle) complex
0.099 9.41E-9 YCR027C RHB1 hom Putative Rheb-related GTPase involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins
0.098 1.26E-8 YGL131C SNT2 hom DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.095 3.07E-8 YML032C RAD52 hom Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis
0.095 3.18E-8 YPL206C PGC1 hom Phosphatidyl Glycerol phospholipase C; regulates the phosphatidylglycerol (PG) content via a phospholipase C-type degradation mechanism; contains glycerophosphodiester phosphodiesterase motifs
0.093 5.86E-8 YCL042W_p YCL042W_p hom Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
0.091 1.10E-7 YMR222C FSH2 hom Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
0.089 2.73E-7 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.088 3.82E-7 YDR387C_p YDR387C_p hom Putative transporter, member of the sugar porter family; YDR387C is not an essential gene
0.087 4.52E-7 YDR184C ATC1 hom Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress
0.085 9.13E-7 YGR019W UGA1 hom Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress
0.084 1.07E-6 YOR008C-A_p YOR008C-A_p hom Putative protein of unknown function, includes a potential transmembrane domain; deletion results in slightly lengthened telomeres
0.082 1.78E-6 YDL055C PSA1 het GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure